Cellular Intrinsic Factors Involved in the Resistance of Squamous Cell Carcinoma to Photodynamic Therapy

Autores
Gilaberte, Yolanda; Milla Sanabria, Laura Natalia; Salazar, Nerea; Vera Alvarez, Jesus; Kourani, Omar; Damian, Alejandra; Rivarola, Viviana; Roca, Maria Jose; Espada, Jesus; Gonzalez, Salvador; Juarranz, Angeles
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Photodynamic therapy (PDT) is widely used to treat non-melanoma skin cancer. However, some patients affected with squamous cell carcinoma (SCC) do not respond adequately to PDT with methyl-δ-aminolevulinic acid (MAL-PDT) and the tumors acquire an infiltrative phenotype and became histologically more aggressive, less differentiated, and more fibroblastic. To search for potential factors implicated in SCC resistance to PDT, we have used the SCC-13 cell line (parental) and resistant SCC-13 cells obtained by repeated MAL-PDT treatments (5th and 10th PDT-resistant generations). Xenografts assays in immunodeficient mice showed that the tumors generated by resistant cells were bigger than those induced by parental cells. Comparative genomic hybridization array (aCGH) showed that the three cell types presented amplicons in 3p12.1 CADM2, 7p11.2 EFGR, and 11q13.3 CCND1 genes. The 5th and 10th PDT-resistant cells showed an amplicon in 5q11.2 MAP3K1, which was not present in parental cells. The changes detected by aCGH on CCND1, EFGR, and MAP3K1 were confirmed in extracts of SCC-13 cells by reverse-transcriptase PCR and by western blot, and by immunohistochemistry in human biopsies from persistent tumors after MAL-PDT. Our data suggest that genomic imbalances related to CCND1, EFGR, and particularly MAP3K1 seem to be involved in the development of the resistance of SCC to PDT.
Fil: Gilaberte, Yolanda. Hospital de San Jorge de Huesca. Zaragoza; España
Fil: Milla Sanabria, Laura Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Salazar, Nerea. Universidad Autonoma de Madrid. Facultad de Ciencias. Departamento de Biología.; España
Fil: Vera Alvarez, Jesus. Hospital de San Jorge de Huesca. Zaragoza; España
Fil: Kourani, Omar. Universidad Autonoma de Madrid. Facultad de Ciencias. Departamento de Biología.; España
Fil: Damian, Alejandra. Universidad Autonoma de Madrid. Facultad de Ciencias. Departamento de Biología.; España
Fil: Rivarola, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Roca, Maria Jose. Hospital de San Jorge de Huesca. Zaragoza; España
Fil: Espada, Jesus. Universidad Autonoma de Madrid. Facultad de Ciencias. Departamento de Biología.; España
Fil: Gonzalez, Salvador. Memorial Sloan-Kettering Cancer Center. New York; Estados Unidos
Fil: Juarranz, Angeles. Universidad Autonoma de Madrid. Facultad de Ciencias. Departamento de Biología.; España
Materia
Photodynamic Therapy
Non Melanoma Skin Cancer
Squamous Cell Carcinoma
Resistance to Pdt
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33782
Acceder
id CONICETDig_5ed26491b714669a8338ced414cf66ed
oai_identifier_str oai:ri.conicet.gov.ar:11336/33782
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cellular Intrinsic Factors Involved in the Resistance of Squamous Cell Carcinoma to Photodynamic TherapyGilaberte, YolandaMilla Sanabria, Laura NataliaSalazar, NereaVera Alvarez, JesusKourani, OmarDamian, AlejandraRivarola, VivianaRoca, Maria JoseEspada, JesusGonzalez, SalvadorJuarranz, AngelesPhotodynamic TherapyNon Melanoma Skin CancerSquamous Cell CarcinomaResistance to Pdthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Photodynamic therapy (PDT) is widely used to treat non-melanoma skin cancer. However, some patients affected with squamous cell carcinoma (SCC) do not respond adequately to PDT with methyl-δ-aminolevulinic acid (MAL-PDT) and the tumors acquire an infiltrative phenotype and became histologically more aggressive, less differentiated, and more fibroblastic. To search for potential factors implicated in SCC resistance to PDT, we have used the SCC-13 cell line (parental) and resistant SCC-13 cells obtained by repeated MAL-PDT treatments (5th and 10th PDT-resistant generations). Xenografts assays in immunodeficient mice showed that the tumors generated by resistant cells were bigger than those induced by parental cells. Comparative genomic hybridization array (aCGH) showed that the three cell types presented amplicons in 3p12.1 CADM2, 7p11.2 EFGR, and 11q13.3 CCND1 genes. The 5th and 10th PDT-resistant cells showed an amplicon in 5q11.2 MAP3K1, which was not present in parental cells. The changes detected by aCGH on CCND1, EFGR, and MAP3K1 were confirmed in extracts of SCC-13 cells by reverse-transcriptase PCR and by western blot, and by immunohistochemistry in human biopsies from persistent tumors after MAL-PDT. Our data suggest that genomic imbalances related to CCND1, EFGR, and particularly MAP3K1 seem to be involved in the development of the resistance of SCC to PDT.Fil: Gilaberte, Yolanda. Hospital de San Jorge de Huesca. Zaragoza; EspañaFil: Milla Sanabria, Laura Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Salazar, Nerea. Universidad Autonoma de Madrid. Facultad de Ciencias. Departamento de Biología.; EspañaFil: Vera Alvarez, Jesus. Hospital de San Jorge de Huesca. Zaragoza; EspañaFil: Kourani, Omar. Universidad Autonoma de Madrid. Facultad de Ciencias. Departamento de Biología.; EspañaFil: Damian, Alejandra. Universidad Autonoma de Madrid. Facultad de Ciencias. Departamento de Biología.; EspañaFil: Rivarola, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Roca, Maria Jose. Hospital de San Jorge de Huesca. Zaragoza; EspañaFil: Espada, Jesus. Universidad Autonoma de Madrid. Facultad de Ciencias. Departamento de Biología.; EspañaFil: Gonzalez, Salvador. Memorial Sloan-Kettering Cancer Center. New York; Estados UnidosFil: Juarranz, Angeles. Universidad Autonoma de Madrid. Facultad de Ciencias. Departamento de Biología.; EspañaNature Publishing Group2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33782Gilaberte, Yolanda; Milla Sanabria, Laura Natalia; Salazar, Nerea; Vera Alvarez, Jesus; Kourani, Omar; et al.; Cellular Intrinsic Factors Involved in the Resistance of Squamous Cell Carcinoma to Photodynamic Therapy; Nature Publishing Group; Journal Of Investigative Dermatology; 134; 9; 3-2014; 2428-24370022-202XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/jid.2014.178info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022202X15369748info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:40:45Zoai:ri.conicet.gov.ar:11336/33782instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:40:45.358CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cellular Intrinsic Factors Involved in the Resistance of Squamous Cell Carcinoma to Photodynamic Therapy
title Cellular Intrinsic Factors Involved in the Resistance of Squamous Cell Carcinoma to Photodynamic Therapy
spellingShingle Cellular Intrinsic Factors Involved in the Resistance of Squamous Cell Carcinoma to Photodynamic Therapy
Gilaberte, Yolanda
Photodynamic Therapy
Non Melanoma Skin Cancer
Squamous Cell Carcinoma
Resistance to Pdt
title_short Cellular Intrinsic Factors Involved in the Resistance of Squamous Cell Carcinoma to Photodynamic Therapy
title_full Cellular Intrinsic Factors Involved in the Resistance of Squamous Cell Carcinoma to Photodynamic Therapy
title_fullStr Cellular Intrinsic Factors Involved in the Resistance of Squamous Cell Carcinoma to Photodynamic Therapy
title_full_unstemmed Cellular Intrinsic Factors Involved in the Resistance of Squamous Cell Carcinoma to Photodynamic Therapy
title_sort Cellular Intrinsic Factors Involved in the Resistance of Squamous Cell Carcinoma to Photodynamic Therapy
dc.creator.none.fl_str_mv Gilaberte, Yolanda
Milla Sanabria, Laura Natalia
Salazar, Nerea
Vera Alvarez, Jesus
Kourani, Omar
Damian, Alejandra
Rivarola, Viviana
Roca, Maria Jose
Espada, Jesus
Gonzalez, Salvador
Juarranz, Angeles
author Gilaberte, Yolanda
author_facet Gilaberte, Yolanda
Milla Sanabria, Laura Natalia
Salazar, Nerea
Vera Alvarez, Jesus
Kourani, Omar
Damian, Alejandra
Rivarola, Viviana
Roca, Maria Jose
Espada, Jesus
Gonzalez, Salvador
Juarranz, Angeles
author_role author
author2 Milla Sanabria, Laura Natalia
Salazar, Nerea
Vera Alvarez, Jesus
Kourani, Omar
Damian, Alejandra
Rivarola, Viviana
Roca, Maria Jose
Espada, Jesus
Gonzalez, Salvador
Juarranz, Angeles
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Photodynamic Therapy
Non Melanoma Skin Cancer
Squamous Cell Carcinoma
Resistance to Pdt
topic Photodynamic Therapy
Non Melanoma Skin Cancer
Squamous Cell Carcinoma
Resistance to Pdt
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Photodynamic therapy (PDT) is widely used to treat non-melanoma skin cancer. However, some patients affected with squamous cell carcinoma (SCC) do not respond adequately to PDT with methyl-δ-aminolevulinic acid (MAL-PDT) and the tumors acquire an infiltrative phenotype and became histologically more aggressive, less differentiated, and more fibroblastic. To search for potential factors implicated in SCC resistance to PDT, we have used the SCC-13 cell line (parental) and resistant SCC-13 cells obtained by repeated MAL-PDT treatments (5th and 10th PDT-resistant generations). Xenografts assays in immunodeficient mice showed that the tumors generated by resistant cells were bigger than those induced by parental cells. Comparative genomic hybridization array (aCGH) showed that the three cell types presented amplicons in 3p12.1 CADM2, 7p11.2 EFGR, and 11q13.3 CCND1 genes. The 5th and 10th PDT-resistant cells showed an amplicon in 5q11.2 MAP3K1, which was not present in parental cells. The changes detected by aCGH on CCND1, EFGR, and MAP3K1 were confirmed in extracts of SCC-13 cells by reverse-transcriptase PCR and by western blot, and by immunohistochemistry in human biopsies from persistent tumors after MAL-PDT. Our data suggest that genomic imbalances related to CCND1, EFGR, and particularly MAP3K1 seem to be involved in the development of the resistance of SCC to PDT.
Fil: Gilaberte, Yolanda. Hospital de San Jorge de Huesca. Zaragoza; España
Fil: Milla Sanabria, Laura Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Salazar, Nerea. Universidad Autonoma de Madrid. Facultad de Ciencias. Departamento de Biología.; España
Fil: Vera Alvarez, Jesus. Hospital de San Jorge de Huesca. Zaragoza; España
Fil: Kourani, Omar. Universidad Autonoma de Madrid. Facultad de Ciencias. Departamento de Biología.; España
Fil: Damian, Alejandra. Universidad Autonoma de Madrid. Facultad de Ciencias. Departamento de Biología.; España
Fil: Rivarola, Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Roca, Maria Jose. Hospital de San Jorge de Huesca. Zaragoza; España
Fil: Espada, Jesus. Universidad Autonoma de Madrid. Facultad de Ciencias. Departamento de Biología.; España
Fil: Gonzalez, Salvador. Memorial Sloan-Kettering Cancer Center. New York; Estados Unidos
Fil: Juarranz, Angeles. Universidad Autonoma de Madrid. Facultad de Ciencias. Departamento de Biología.; España
description Photodynamic therapy (PDT) is widely used to treat non-melanoma skin cancer. However, some patients affected with squamous cell carcinoma (SCC) do not respond adequately to PDT with methyl-δ-aminolevulinic acid (MAL-PDT) and the tumors acquire an infiltrative phenotype and became histologically more aggressive, less differentiated, and more fibroblastic. To search for potential factors implicated in SCC resistance to PDT, we have used the SCC-13 cell line (parental) and resistant SCC-13 cells obtained by repeated MAL-PDT treatments (5th and 10th PDT-resistant generations). Xenografts assays in immunodeficient mice showed that the tumors generated by resistant cells were bigger than those induced by parental cells. Comparative genomic hybridization array (aCGH) showed that the three cell types presented amplicons in 3p12.1 CADM2, 7p11.2 EFGR, and 11q13.3 CCND1 genes. The 5th and 10th PDT-resistant cells showed an amplicon in 5q11.2 MAP3K1, which was not present in parental cells. The changes detected by aCGH on CCND1, EFGR, and MAP3K1 were confirmed in extracts of SCC-13 cells by reverse-transcriptase PCR and by western blot, and by immunohistochemistry in human biopsies from persistent tumors after MAL-PDT. Our data suggest that genomic imbalances related to CCND1, EFGR, and particularly MAP3K1 seem to be involved in the development of the resistance of SCC to PDT.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33782
Gilaberte, Yolanda; Milla Sanabria, Laura Natalia; Salazar, Nerea; Vera Alvarez, Jesus; Kourani, Omar; et al.; Cellular Intrinsic Factors Involved in the Resistance of Squamous Cell Carcinoma to Photodynamic Therapy; Nature Publishing Group; Journal Of Investigative Dermatology; 134; 9; 3-2014; 2428-2437
0022-202X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33782
identifier_str_mv Gilaberte, Yolanda; Milla Sanabria, Laura Natalia; Salazar, Nerea; Vera Alvarez, Jesus; Kourani, Omar; et al.; Cellular Intrinsic Factors Involved in the Resistance of Squamous Cell Carcinoma to Photodynamic Therapy; Nature Publishing Group; Journal Of Investigative Dermatology; 134; 9; 3-2014; 2428-2437
0022-202X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/jid.2014.178
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022202X15369748
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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