Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration

Autores
Biglione, Catalina; Bergueiro, Julian; Asadian Birjand, Mazdak; Weise, Christoph; Khobragade, Vrushali; Chate, Govind; Dongare, Manoj; Khandare, Jayant; Strumia, Miriam Cristina; Calderon, Marcelo
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Magnetic nanogels (MNGs) are designed to have all the required features for their use as highly efficient trapping materials in the challenging task of selectively capturing circulating tumor cells (CTCs) from the bloodstream. Advantageously, the discrimination of CTCs from hematological cells, which is a key factor in the capturing process, can be optimized by finely tuning the polymers used to link the targeting moiety to the MNG. We describe herein the relationship between the capturing efficiency of CTCs with overexpressed transferrin receptors and the different strategies on the polymer used as linker to decorate these MNGs with transferrin (Tf). Heterobifunctional polyethylene glycol (PEG) linkers with different molecular weights were coupled to Tf in different ratios. Optimal values over 80% CTC capture efficiency were obtained when 3 PEG linkers with a length of 8 ethylene glycol (EG) units were used, which reveals the important role of the linker in the design of a CTC-sorting system.
Fil: Biglione, Catalina. Universidad Nacional de Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; Argentina
Fil: Bergueiro, Julian. Freie Universität Berlin; Alemania
Fil: Asadian Birjand, Mazdak. Freie Universität Berlin; Alemania
Fil: Weise, Christoph. Freie Universität Berlin; Alemania
Fil: Khobragade, Vrushali. Manik Hospital and Research Center; India. Actorius Innovations and Research; India
Fil: Chate, Govind. Maeer's Maharashtra Institute of Pharmacy; India
Fil: Dongare, Manoj. Maeer's Maharashtra Institute of Pharmacy; India. Actorius Innovations and Research; India. Manik Hospital and Research Center; India
Fil: Khandare, Jayant. Maeer's Maharashtra Institute of Pharmacy; India. Actorius Innovations and Research; India. Manik Hospital and Research Center; India
Fil: Strumia, Miriam Cristina. Universidad Nacional de Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; Argentina
Fil: Calderon, Marcelo. Freie Universität Berlin; Alemania
Materia
CELL SORTING
CIRCULATING TUMOR CELLS
MAGNETIC NANOGEL
TRANSFERRIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/91443
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/91443
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin DecorationBiglione, CatalinaBergueiro, JulianAsadian Birjand, MazdakWeise, ChristophKhobragade, VrushaliChate, GovindDongare, ManojKhandare, JayantStrumia, Miriam CristinaCalderon, MarceloCELL SORTINGCIRCULATING TUMOR CELLSMAGNETIC NANOGELTRANSFERRINhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Magnetic nanogels (MNGs) are designed to have all the required features for their use as highly efficient trapping materials in the challenging task of selectively capturing circulating tumor cells (CTCs) from the bloodstream. Advantageously, the discrimination of CTCs from hematological cells, which is a key factor in the capturing process, can be optimized by finely tuning the polymers used to link the targeting moiety to the MNG. We describe herein the relationship between the capturing efficiency of CTCs with overexpressed transferrin receptors and the different strategies on the polymer used as linker to decorate these MNGs with transferrin (Tf). Heterobifunctional polyethylene glycol (PEG) linkers with different molecular weights were coupled to Tf in different ratios. Optimal values over 80% CTC capture efficiency were obtained when 3 PEG linkers with a length of 8 ethylene glycol (EG) units were used, which reveals the important role of the linker in the design of a CTC-sorting system.Fil: Biglione, Catalina. Universidad Nacional de Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; ArgentinaFil: Bergueiro, Julian. Freie Universität Berlin; AlemaniaFil: Asadian Birjand, Mazdak. Freie Universität Berlin; AlemaniaFil: Weise, Christoph. Freie Universität Berlin; AlemaniaFil: Khobragade, Vrushali. Manik Hospital and Research Center; India. Actorius Innovations and Research; IndiaFil: Chate, Govind. Maeer's Maharashtra Institute of Pharmacy; IndiaFil: Dongare, Manoj. Maeer's Maharashtra Institute of Pharmacy; India. Actorius Innovations and Research; India. Manik Hospital and Research Center; IndiaFil: Khandare, Jayant. Maeer's Maharashtra Institute of Pharmacy; India. Actorius Innovations and Research; India. Manik Hospital and Research Center; IndiaFil: Strumia, Miriam Cristina. Universidad Nacional de Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; ArgentinaFil: Calderon, Marcelo. Freie Universität Berlin; AlemaniaMDPI AG2018-02-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/91443Biglione, Catalina; Bergueiro, Julian; Asadian Birjand, Mazdak; Weise, Christoph; Khobragade, Vrushali; et al.; Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration; MDPI AG; Polymers; 10; 2; 11-2-2018; 1-142073-4360CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/polym10020174info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4360/10/2/174info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:30:02Zoai:ri.conicet.gov.ar:11336/91443instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:30:02.24CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration
title Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration
spellingShingle Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration
Biglione, Catalina
CELL SORTING
CIRCULATING TUMOR CELLS
MAGNETIC NANOGEL
TRANSFERRIN
title_short Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration
title_full Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration
title_fullStr Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration
title_full_unstemmed Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration
title_sort Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration
dc.creator.none.fl_str_mv Biglione, Catalina
Bergueiro, Julian
Asadian Birjand, Mazdak
Weise, Christoph
Khobragade, Vrushali
Chate, Govind
Dongare, Manoj
Khandare, Jayant
Strumia, Miriam Cristina
Calderon, Marcelo
author Biglione, Catalina
author_facet Biglione, Catalina
Bergueiro, Julian
Asadian Birjand, Mazdak
Weise, Christoph
Khobragade, Vrushali
Chate, Govind
Dongare, Manoj
Khandare, Jayant
Strumia, Miriam Cristina
Calderon, Marcelo
author_role author
author2 Bergueiro, Julian
Asadian Birjand, Mazdak
Weise, Christoph
Khobragade, Vrushali
Chate, Govind
Dongare, Manoj
Khandare, Jayant
Strumia, Miriam Cristina
Calderon, Marcelo
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CELL SORTING
CIRCULATING TUMOR CELLS
MAGNETIC NANOGEL
TRANSFERRIN
topic CELL SORTING
CIRCULATING TUMOR CELLS
MAGNETIC NANOGEL
TRANSFERRIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Magnetic nanogels (MNGs) are designed to have all the required features for their use as highly efficient trapping materials in the challenging task of selectively capturing circulating tumor cells (CTCs) from the bloodstream. Advantageously, the discrimination of CTCs from hematological cells, which is a key factor in the capturing process, can be optimized by finely tuning the polymers used to link the targeting moiety to the MNG. We describe herein the relationship between the capturing efficiency of CTCs with overexpressed transferrin receptors and the different strategies on the polymer used as linker to decorate these MNGs with transferrin (Tf). Heterobifunctional polyethylene glycol (PEG) linkers with different molecular weights were coupled to Tf in different ratios. Optimal values over 80% CTC capture efficiency were obtained when 3 PEG linkers with a length of 8 ethylene glycol (EG) units were used, which reveals the important role of the linker in the design of a CTC-sorting system.
Fil: Biglione, Catalina. Universidad Nacional de Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; Argentina
Fil: Bergueiro, Julian. Freie Universität Berlin; Alemania
Fil: Asadian Birjand, Mazdak. Freie Universität Berlin; Alemania
Fil: Weise, Christoph. Freie Universität Berlin; Alemania
Fil: Khobragade, Vrushali. Manik Hospital and Research Center; India. Actorius Innovations and Research; India
Fil: Chate, Govind. Maeer's Maharashtra Institute of Pharmacy; India
Fil: Dongare, Manoj. Maeer's Maharashtra Institute of Pharmacy; India. Actorius Innovations and Research; India. Manik Hospital and Research Center; India
Fil: Khandare, Jayant. Maeer's Maharashtra Institute of Pharmacy; India. Actorius Innovations and Research; India. Manik Hospital and Research Center; India
Fil: Strumia, Miriam Cristina. Universidad Nacional de Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; Argentina
Fil: Calderon, Marcelo. Freie Universität Berlin; Alemania
description Magnetic nanogels (MNGs) are designed to have all the required features for their use as highly efficient trapping materials in the challenging task of selectively capturing circulating tumor cells (CTCs) from the bloodstream. Advantageously, the discrimination of CTCs from hematological cells, which is a key factor in the capturing process, can be optimized by finely tuning the polymers used to link the targeting moiety to the MNG. We describe herein the relationship between the capturing efficiency of CTCs with overexpressed transferrin receptors and the different strategies on the polymer used as linker to decorate these MNGs with transferrin (Tf). Heterobifunctional polyethylene glycol (PEG) linkers with different molecular weights were coupled to Tf in different ratios. Optimal values over 80% CTC capture efficiency were obtained when 3 PEG linkers with a length of 8 ethylene glycol (EG) units were used, which reveals the important role of the linker in the design of a CTC-sorting system.
publishDate 2018
dc.date.none.fl_str_mv 2018-02-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/91443
Biglione, Catalina; Bergueiro, Julian; Asadian Birjand, Mazdak; Weise, Christoph; Khobragade, Vrushali; et al.; Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration; MDPI AG; Polymers; 10; 2; 11-2-2018; 1-14
2073-4360
CONICET Digital
CONICET
url http://hdl.handle.net/11336/91443
identifier_str_mv Biglione, Catalina; Bergueiro, Julian; Asadian Birjand, Mazdak; Weise, Christoph; Khobragade, Vrushali; et al.; Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration; MDPI AG; Polymers; 10; 2; 11-2-2018; 1-14
2073-4360
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/polym10020174
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4360/10/2/174
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614308575576064
score 13.070432

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