Biosynthesis of very long chain fatty acid-containing psphingolipids in germinal cells

Autores
Furland, Natalia Edith; Oresti, Gerardo Martin; Aveldaño, Marta Isabel
Año de publicación
2011
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In mammalian testis, sphingomyelin (SM) and ceramide (Cer) were previously shown to be rich in very long chain (VLC) polyunsaturated fatty acids (PUFA). Recent work in rat pachytene spermatocytes and round spermatids demonstrated that these sphingolipids exclusively belong to germ cells. We also recently showed that these two cell types, highly enriched in 22:5n-6- containing glycerophospholipids, express mRNA transcripts of ELOVL5 and ELOVL2, two elongases involved in the biosynthesis of C18-C22 PUFA. Because such type of PUFA need to be further elongated to produce the VLCPUFA present in rat testicular sphingolipids (C26-C32), in this work we studied the expression of ELOVL4, the enzyme expected to catalyze such elongation. During postnatal development, ELOVL4 protein expression was first detectable in the rat testis at P25, in concomitance with the timing of appearance of the first spermatocytes. In adult rat seminiferous tubules, whereas the expression of this elongase was found to be only marginal in Sertoli cells, it was significant in spermatocytes and spermatids. The use of radiolabeled precursors and the fluorescent marker NBD C6-Cer showed that isolated spermatogenic cells in culture do synthesize sphingolipids. Both results are consistent in demonstrating that spermatogenic cells are able to biosynthesize the molecular species of SM and Cer that contain VLCPUFA.
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XLVII Reunión Nacional de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Potrero de los Funes
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
Germ cells
Sphingolipid biosynthesis
Glycosphingolipid labeling
Radioactive precursors
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/225481
Acceder
id CONICETDig_04b0d06bd56719c06c52b2b7f9ac2f31
oai_identifier_str oai:ri.conicet.gov.ar:11336/225481
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biosynthesis of very long chain fatty acid-containing psphingolipids in germinal cellsFurland, Natalia EdithOresti, Gerardo MartinAveldaño, Marta IsabelGerm cellsSphingolipid biosynthesisGlycosphingolipid labelingRadioactive precursorshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In mammalian testis, sphingomyelin (SM) and ceramide (Cer) were previously shown to be rich in very long chain (VLC) polyunsaturated fatty acids (PUFA). Recent work in rat pachytene spermatocytes and round spermatids demonstrated that these sphingolipids exclusively belong to germ cells. We also recently showed that these two cell types, highly enriched in 22:5n-6- containing glycerophospholipids, express mRNA transcripts of ELOVL5 and ELOVL2, two elongases involved in the biosynthesis of C18-C22 PUFA. Because such type of PUFA need to be further elongated to produce the VLCPUFA present in rat testicular sphingolipids (C26-C32), in this work we studied the expression of ELOVL4, the enzyme expected to catalyze such elongation. During postnatal development, ELOVL4 protein expression was first detectable in the rat testis at P25, in concomitance with the timing of appearance of the first spermatocytes. In adult rat seminiferous tubules, whereas the expression of this elongase was found to be only marginal in Sertoli cells, it was significant in spermatocytes and spermatids. The use of radiolabeled precursors and the fluorescent marker NBD C6-Cer showed that isolated spermatogenic cells in culture do synthesize sphingolipids. Both results are consistent in demonstrating that spermatogenic cells are able to biosynthesize the molecular species of SM and Cer that contain VLCPUFA.Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaXLVII Reunión Nacional de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularPotrero de los FunesArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularSociedad Argentina de Investigación Bioquímica y Biología Molecular2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/225481Biosynthesis of very long chain fatty acid-containing psphingolipids in germinal cells; XLVII Reunión Nacional de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes; Argentina; 2011; 50-500327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-35.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:28Zoai:ri.conicet.gov.ar:11336/225481instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:28.692CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biosynthesis of very long chain fatty acid-containing psphingolipids in germinal cells
title Biosynthesis of very long chain fatty acid-containing psphingolipids in germinal cells
spellingShingle Biosynthesis of very long chain fatty acid-containing psphingolipids in germinal cells
Furland, Natalia Edith
Germ cells
Sphingolipid biosynthesis
Glycosphingolipid labeling
Radioactive precursors
title_short Biosynthesis of very long chain fatty acid-containing psphingolipids in germinal cells
title_full Biosynthesis of very long chain fatty acid-containing psphingolipids in germinal cells
title_fullStr Biosynthesis of very long chain fatty acid-containing psphingolipids in germinal cells
title_full_unstemmed Biosynthesis of very long chain fatty acid-containing psphingolipids in germinal cells
title_sort Biosynthesis of very long chain fatty acid-containing psphingolipids in germinal cells
dc.creator.none.fl_str_mv Furland, Natalia Edith
Oresti, Gerardo Martin
Aveldaño, Marta Isabel
author Furland, Natalia Edith
author_facet Furland, Natalia Edith
Oresti, Gerardo Martin
Aveldaño, Marta Isabel
author_role author
author2 Oresti, Gerardo Martin
Aveldaño, Marta Isabel
author2_role author
author
dc.subject.none.fl_str_mv Germ cells
Sphingolipid biosynthesis
Glycosphingolipid labeling
Radioactive precursors
topic Germ cells
Sphingolipid biosynthesis
Glycosphingolipid labeling
Radioactive precursors
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In mammalian testis, sphingomyelin (SM) and ceramide (Cer) were previously shown to be rich in very long chain (VLC) polyunsaturated fatty acids (PUFA). Recent work in rat pachytene spermatocytes and round spermatids demonstrated that these sphingolipids exclusively belong to germ cells. We also recently showed that these two cell types, highly enriched in 22:5n-6- containing glycerophospholipids, express mRNA transcripts of ELOVL5 and ELOVL2, two elongases involved in the biosynthesis of C18-C22 PUFA. Because such type of PUFA need to be further elongated to produce the VLCPUFA present in rat testicular sphingolipids (C26-C32), in this work we studied the expression of ELOVL4, the enzyme expected to catalyze such elongation. During postnatal development, ELOVL4 protein expression was first detectable in the rat testis at P25, in concomitance with the timing of appearance of the first spermatocytes. In adult rat seminiferous tubules, whereas the expression of this elongase was found to be only marginal in Sertoli cells, it was significant in spermatocytes and spermatids. The use of radiolabeled precursors and the fluorescent marker NBD C6-Cer showed that isolated spermatogenic cells in culture do synthesize sphingolipids. Both results are consistent in demonstrating that spermatogenic cells are able to biosynthesize the molecular species of SM and Cer that contain VLCPUFA.
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XLVII Reunión Nacional de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Potrero de los Funes
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description In mammalian testis, sphingomyelin (SM) and ceramide (Cer) were previously shown to be rich in very long chain (VLC) polyunsaturated fatty acids (PUFA). Recent work in rat pachytene spermatocytes and round spermatids demonstrated that these sphingolipids exclusively belong to germ cells. We also recently showed that these two cell types, highly enriched in 22:5n-6- containing glycerophospholipids, express mRNA transcripts of ELOVL5 and ELOVL2, two elongases involved in the biosynthesis of C18-C22 PUFA. Because such type of PUFA need to be further elongated to produce the VLCPUFA present in rat testicular sphingolipids (C26-C32), in this work we studied the expression of ELOVL4, the enzyme expected to catalyze such elongation. During postnatal development, ELOVL4 protein expression was first detectable in the rat testis at P25, in concomitance with the timing of appearance of the first spermatocytes. In adult rat seminiferous tubules, whereas the expression of this elongase was found to be only marginal in Sertoli cells, it was significant in spermatocytes and spermatids. The use of radiolabeled precursors and the fluorescent marker NBD C6-Cer showed that isolated spermatogenic cells in culture do synthesize sphingolipids. Both results are consistent in demonstrating that spermatogenic cells are able to biosynthesize the molecular species of SM and Cer that contain VLCPUFA.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/225481
Biosynthesis of very long chain fatty acid-containing psphingolipids in germinal cells; XLVII Reunión Nacional de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes; Argentina; 2011; 50-50
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/225481
identifier_str_mv Biosynthesis of very long chain fatty acid-containing psphingolipids in germinal cells; XLVII Reunión Nacional de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes; Argentina; 2011; 50-50
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-35.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación Bioquímica y Biología Molecular
publisher.none.fl_str_mv Sociedad Argentina de Investigación Bioquímica y Biología Molecular
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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