Alkaline phosphatase in shrimp Artemesia Longinaris: Response to feed

Autores
Pereira, Nair de Los Angeles; Fernandez Gimenez, Analia Veronica; Haran, Nora Selma; Fenucci, Jorge Lino
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Abstract: The purpose of this study was to evaluate the alkaline phosphatase activity in haemocytes and midgut gland of the argentine shrimp Artemesia longinaris in relationship with different doses of vitamin D3 in feed and to estimate its potential use as biomarker for nutritional stress. A nine-week trial was carried out with juvenile shrimps in aquaria. Animals were fed on semipurified feeds with different levels of vitamin D3 (0; 0.2; 0.375; 0.75 and 1 mg vitamin D3 kg-1 feed). Treatments without vitamin D in haemocytes revealed the significantly highest protein content (P<0.05), as the lowest values were recorded on 0.2 and 1 mg vitamin D kg-1 treatments. The analysis of protein content of midgut glands revealed a maximum content for diets containing 0.2 and 0.75 mg Vitamin D kg-1. The optimum pH value for alkaline phosphatase in midgut gland of A. longinaris was 9.5. The results show the occurrence of alkaline phosphatase activity in the tissues of shrimp A. longinaris being studied, describing higher enzymatic activity values in haemocytes than midgut gland. The highest enzyme activity in haemocytes was observed on shrimps fed without vitamin D (1.235 abs min-1 mg protein-1), however, in midgut gland, the activity varied from 0.141 to 0.297 abs min-1 mg protein-1, with the highest values on 0 and 1 mg vitamin D kg-1. On the other hand, histological analysis of the midgut gland confirmed a good health of the shrimp fed 0.375 and 0.750 mg vitamin D kg-1 that is why they were used as optimal values for determining enzymatic activity, shrimp fed diets without vitamin D or 1 mg kg-1 showed signs of malnourishment. The results indicate that alkaline phosphatase activity in A. longinaris was influenced by vitamin D in feed and may be used as a biomonitor of nutritional stress.
Fil: Pereira, Nair de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Fernandez Gimenez, Analia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Haran, Nora Selma. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas; Argentina
Fil: Fenucci, Jorge Lino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Materia
Alkaline Phosphatase
Midgut Gland
Shrimp
Vitamin D
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/71736
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/71736
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Alkaline phosphatase in shrimp Artemesia Longinaris: Response to feedPereira, Nair de Los AngelesFernandez Gimenez, Analia VeronicaHaran, Nora SelmaFenucci, Jorge LinoAlkaline PhosphataseMidgut GlandShrimpVitamin Dhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Abstract: The purpose of this study was to evaluate the alkaline phosphatase activity in haemocytes and midgut gland of the argentine shrimp Artemesia longinaris in relationship with different doses of vitamin D3 in feed and to estimate its potential use as biomarker for nutritional stress. A nine-week trial was carried out with juvenile shrimps in aquaria. Animals were fed on semipurified feeds with different levels of vitamin D3 (0; 0.2; 0.375; 0.75 and 1 mg vitamin D3 kg-1 feed). Treatments without vitamin D in haemocytes revealed the significantly highest protein content (P<0.05), as the lowest values were recorded on 0.2 and 1 mg vitamin D kg-1 treatments. The analysis of protein content of midgut glands revealed a maximum content for diets containing 0.2 and 0.75 mg Vitamin D kg-1. The optimum pH value for alkaline phosphatase in midgut gland of A. longinaris was 9.5. The results show the occurrence of alkaline phosphatase activity in the tissues of shrimp A. longinaris being studied, describing higher enzymatic activity values in haemocytes than midgut gland. The highest enzyme activity in haemocytes was observed on shrimps fed without vitamin D (1.235 abs min-1 mg protein-1), however, in midgut gland, the activity varied from 0.141 to 0.297 abs min-1 mg protein-1, with the highest values on 0 and 1 mg vitamin D kg-1. On the other hand, histological analysis of the midgut gland confirmed a good health of the shrimp fed 0.375 and 0.750 mg vitamin D kg-1 that is why they were used as optimal values for determining enzymatic activity, shrimp fed diets without vitamin D or 1 mg kg-1 showed signs of malnourishment. The results indicate that alkaline phosphatase activity in A. longinaris was influenced by vitamin D in feed and may be used as a biomonitor of nutritional stress.Fil: Pereira, Nair de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Fernandez Gimenez, Analia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Haran, Nora Selma. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas; ArgentinaFil: Fenucci, Jorge Lino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaAlireza Asem2013-06-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71736Pereira, Nair de Los Angeles; Fernandez Gimenez, Analia Veronica; Haran, Nora Selma; Fenucci, Jorge Lino; Alkaline phosphatase in shrimp Artemesia Longinaris: Response to feed; Alireza Asem; International Journal of Aquatic Science; 4; 2; 27-6-2013; 3-132008-8019CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.journal-aquaticscience.com/article_73501.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:59Zoai:ri.conicet.gov.ar:11336/71736instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:59.746CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Alkaline phosphatase in shrimp Artemesia Longinaris: Response to feed
title Alkaline phosphatase in shrimp Artemesia Longinaris: Response to feed
spellingShingle Alkaline phosphatase in shrimp Artemesia Longinaris: Response to feed
Pereira, Nair de Los Angeles
Alkaline Phosphatase
Midgut Gland
Shrimp
Vitamin D
title_short Alkaline phosphatase in shrimp Artemesia Longinaris: Response to feed
title_full Alkaline phosphatase in shrimp Artemesia Longinaris: Response to feed
title_fullStr Alkaline phosphatase in shrimp Artemesia Longinaris: Response to feed
title_full_unstemmed Alkaline phosphatase in shrimp Artemesia Longinaris: Response to feed
title_sort Alkaline phosphatase in shrimp Artemesia Longinaris: Response to feed
dc.creator.none.fl_str_mv Pereira, Nair de Los Angeles
Fernandez Gimenez, Analia Veronica
Haran, Nora Selma
Fenucci, Jorge Lino
author Pereira, Nair de Los Angeles
author_facet Pereira, Nair de Los Angeles
Fernandez Gimenez, Analia Veronica
Haran, Nora Selma
Fenucci, Jorge Lino
author_role author
author2 Fernandez Gimenez, Analia Veronica
Haran, Nora Selma
Fenucci, Jorge Lino
author2_role author
author
author
dc.subject.none.fl_str_mv Alkaline Phosphatase
Midgut Gland
Shrimp
Vitamin D
topic Alkaline Phosphatase
Midgut Gland
Shrimp
Vitamin D
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Abstract: The purpose of this study was to evaluate the alkaline phosphatase activity in haemocytes and midgut gland of the argentine shrimp Artemesia longinaris in relationship with different doses of vitamin D3 in feed and to estimate its potential use as biomarker for nutritional stress. A nine-week trial was carried out with juvenile shrimps in aquaria. Animals were fed on semipurified feeds with different levels of vitamin D3 (0; 0.2; 0.375; 0.75 and 1 mg vitamin D3 kg-1 feed). Treatments without vitamin D in haemocytes revealed the significantly highest protein content (P<0.05), as the lowest values were recorded on 0.2 and 1 mg vitamin D kg-1 treatments. The analysis of protein content of midgut glands revealed a maximum content for diets containing 0.2 and 0.75 mg Vitamin D kg-1. The optimum pH value for alkaline phosphatase in midgut gland of A. longinaris was 9.5. The results show the occurrence of alkaline phosphatase activity in the tissues of shrimp A. longinaris being studied, describing higher enzymatic activity values in haemocytes than midgut gland. The highest enzyme activity in haemocytes was observed on shrimps fed without vitamin D (1.235 abs min-1 mg protein-1), however, in midgut gland, the activity varied from 0.141 to 0.297 abs min-1 mg protein-1, with the highest values on 0 and 1 mg vitamin D kg-1. On the other hand, histological analysis of the midgut gland confirmed a good health of the shrimp fed 0.375 and 0.750 mg vitamin D kg-1 that is why they were used as optimal values for determining enzymatic activity, shrimp fed diets without vitamin D or 1 mg kg-1 showed signs of malnourishment. The results indicate that alkaline phosphatase activity in A. longinaris was influenced by vitamin D in feed and may be used as a biomonitor of nutritional stress.
Fil: Pereira, Nair de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Fernandez Gimenez, Analia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Haran, Nora Selma. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas; Argentina
Fil: Fenucci, Jorge Lino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
description Abstract: The purpose of this study was to evaluate the alkaline phosphatase activity in haemocytes and midgut gland of the argentine shrimp Artemesia longinaris in relationship with different doses of vitamin D3 in feed and to estimate its potential use as biomarker for nutritional stress. A nine-week trial was carried out with juvenile shrimps in aquaria. Animals were fed on semipurified feeds with different levels of vitamin D3 (0; 0.2; 0.375; 0.75 and 1 mg vitamin D3 kg-1 feed). Treatments without vitamin D in haemocytes revealed the significantly highest protein content (P<0.05), as the lowest values were recorded on 0.2 and 1 mg vitamin D kg-1 treatments. The analysis of protein content of midgut glands revealed a maximum content for diets containing 0.2 and 0.75 mg Vitamin D kg-1. The optimum pH value for alkaline phosphatase in midgut gland of A. longinaris was 9.5. The results show the occurrence of alkaline phosphatase activity in the tissues of shrimp A. longinaris being studied, describing higher enzymatic activity values in haemocytes than midgut gland. The highest enzyme activity in haemocytes was observed on shrimps fed without vitamin D (1.235 abs min-1 mg protein-1), however, in midgut gland, the activity varied from 0.141 to 0.297 abs min-1 mg protein-1, with the highest values on 0 and 1 mg vitamin D kg-1. On the other hand, histological analysis of the midgut gland confirmed a good health of the shrimp fed 0.375 and 0.750 mg vitamin D kg-1 that is why they were used as optimal values for determining enzymatic activity, shrimp fed diets without vitamin D or 1 mg kg-1 showed signs of malnourishment. The results indicate that alkaline phosphatase activity in A. longinaris was influenced by vitamin D in feed and may be used as a biomonitor of nutritional stress.
publishDate 2013
dc.date.none.fl_str_mv 2013-06-27
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71736
Pereira, Nair de Los Angeles; Fernandez Gimenez, Analia Veronica; Haran, Nora Selma; Fenucci, Jorge Lino; Alkaline phosphatase in shrimp Artemesia Longinaris: Response to feed; Alireza Asem; International Journal of Aquatic Science; 4; 2; 27-6-2013; 3-13
2008-8019
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71736
identifier_str_mv Pereira, Nair de Los Angeles; Fernandez Gimenez, Analia Veronica; Haran, Nora Selma; Fenucci, Jorge Lino; Alkaline phosphatase in shrimp Artemesia Longinaris: Response to feed; Alireza Asem; International Journal of Aquatic Science; 4; 2; 27-6-2013; 3-13
2008-8019
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.journal-aquaticscience.com/article_73501.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Alireza Asem
publisher.none.fl_str_mv Alireza Asem
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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