Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression

Autores
Panero, Julieta; Stanganelli, Carmen; Arbelbide, Jorge; Fantl, Dorotea Beatriz; Kohan, Dana; Garcia Rivello, Hernan; Rabinovich, Gabriel Adrián; Slavutsky, Irma Rosa
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study we analyzed the expression profile and the interactions of all shelterin components in patients with MM and MGUS, and correlated the results with hTERT expression, telomere length (TL) and clinical characteristics of the diseases. Bone marrow samples of 154 patients: 70 with MGUS and 84 with MM were studied. Gene expression was determined by real-time quantitative RT-PCR and TL was evaluated by terminal restriction fragments. A significant overexpression of TRF2, POT1 and hTERT in MM compared to MGUS was observed (p<0.03). Due to the great heterogeneity in hTERT levels, patients were divided into three groups of expression: GI (low), GII (intermediate) and GIII (high). This classification showed an upregulation of TRF2 and RAP1 in MGUS (GIII vs. GI: p<0.01), and an increased expression of TRF2 and TPP1 in MM (GIII vs. GI and GII: p<0.04). In addition, a significant over-expression of LGALS1 (encoding galectin-1, a multifunctional β-galactoside binding protein) was observed in GII/III respect to GI (p=0.013). TL was inversely correlated with telomerase expression (GII vs. GI: p=0.02). In MM, POT1 were positively correlated with β2microglobulin (β2M), advanced DS stages, high calcium levels and bone lesions (p=0.009), whereas TL was correlated positively with hemoglobin levels and negatively with β2M (p=0.02). Our findings provide the first evidence of a global modification in the expression of the six shelterin subunits, their interactions and the correlation between hTERT and LSGAL1 in plasma cell disorders. The strong association of POT1 with different parameters of adverse clinical outcome suggests its importance as a target for novel therapeutic strategies.
Fil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental; Argentina
Fil: Stanganelli, Carmen. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "mariano R. Castex"; Argentina
Fil: Arbelbide, Jorge. Hospital Italiano de Buenos Aires; Argentina
Fil: Fantl, Dorotea Beatriz. Hospital Italiano de Buenos Aires; Argentina
Fil: Kohan, Dana. Hospital Italiano de Buenos Aires; Argentina
Fil: Garcia Rivello, Hernan. Hospital Italiano de Buenos Aires; Argentina
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental; Argentina
Materia
Multiple Myeloma
Mgus
Telomere-Associated Genes
Pot1 Expression
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7635
Acceder
id CONICETDig_88c1386fce572c22fbeafeda85c396fb
oai_identifier_str oai:ri.conicet.gov.ar:11336/7635
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpressionPanero, JulietaStanganelli, CarmenArbelbide, JorgeFantl, Dorotea BeatrizKohan, DanaGarcia Rivello, HernanRabinovich, Gabriel AdriánSlavutsky, Irma RosaMultiple MyelomaMgusTelomere-Associated GenesPot1 Expressionhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In this study we analyzed the expression profile and the interactions of all shelterin components in patients with MM and MGUS, and correlated the results with hTERT expression, telomere length (TL) and clinical characteristics of the diseases. Bone marrow samples of 154 patients: 70 with MGUS and 84 with MM were studied. Gene expression was determined by real-time quantitative RT-PCR and TL was evaluated by terminal restriction fragments. A significant overexpression of TRF2, POT1 and hTERT in MM compared to MGUS was observed (p<0.03). Due to the great heterogeneity in hTERT levels, patients were divided into three groups of expression: GI (low), GII (intermediate) and GIII (high). This classification showed an upregulation of TRF2 and RAP1 in MGUS (GIII vs. GI: p<0.01), and an increased expression of TRF2 and TPP1 in MM (GIII vs. GI and GII: p<0.04). In addition, a significant over-expression of LGALS1 (encoding galectin-1, a multifunctional β-galactoside binding protein) was observed in GII/III respect to GI (p=0.013). TL was inversely correlated with telomerase expression (GII vs. GI: p=0.02). In MM, POT1 were positively correlated with β2microglobulin (β2M), advanced DS stages, high calcium levels and bone lesions (p=0.009), whereas TL was correlated positively with hemoglobin levels and negatively with β2M (p=0.02). Our findings provide the first evidence of a global modification in the expression of the six shelterin subunits, their interactions and the correlation between hTERT and LSGAL1 in plasma cell disorders. The strong association of POT1 with different parameters of adverse clinical outcome suggests its importance as a target for novel therapeutic strategies.Fil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental; ArgentinaFil: Stanganelli, Carmen. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "mariano R. Castex"; ArgentinaFil: Arbelbide, Jorge. Hospital Italiano de Buenos Aires; ArgentinaFil: Fantl, Dorotea Beatriz. Hospital Italiano de Buenos Aires; ArgentinaFil: Kohan, Dana. Hospital Italiano de Buenos Aires; ArgentinaFil: Garcia Rivello, Hernan. Hospital Italiano de Buenos Aires; ArgentinaFil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental; ArgentinaElsevier2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7635Panero, Julieta; Stanganelli, Carmen; Arbelbide, Jorge; Fantl, Dorotea Beatriz; Kohan, Dana; et al.; Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression; Elsevier; Blood Cells Molecules And Diseases; 52; 2-3; 2-2014; 134-1391079-9796enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1079979613002313info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bcmd.2013.10.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:26Zoai:ri.conicet.gov.ar:11336/7635instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:26.276CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression
title Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression
spellingShingle Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression
Panero, Julieta
Multiple Myeloma
Mgus
Telomere-Associated Genes
Pot1 Expression
title_short Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression
title_full Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression
title_fullStr Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression
title_full_unstemmed Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression
title_sort Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression
dc.creator.none.fl_str_mv Panero, Julieta
Stanganelli, Carmen
Arbelbide, Jorge
Fantl, Dorotea Beatriz
Kohan, Dana
Garcia Rivello, Hernan
Rabinovich, Gabriel Adrián
Slavutsky, Irma Rosa
author Panero, Julieta
author_facet Panero, Julieta
Stanganelli, Carmen
Arbelbide, Jorge
Fantl, Dorotea Beatriz
Kohan, Dana
Garcia Rivello, Hernan
Rabinovich, Gabriel Adrián
Slavutsky, Irma Rosa
author_role author
author2 Stanganelli, Carmen
Arbelbide, Jorge
Fantl, Dorotea Beatriz
Kohan, Dana
Garcia Rivello, Hernan
Rabinovich, Gabriel Adrián
Slavutsky, Irma Rosa
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Multiple Myeloma
Mgus
Telomere-Associated Genes
Pot1 Expression
topic Multiple Myeloma
Mgus
Telomere-Associated Genes
Pot1 Expression
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In this study we analyzed the expression profile and the interactions of all shelterin components in patients with MM and MGUS, and correlated the results with hTERT expression, telomere length (TL) and clinical characteristics of the diseases. Bone marrow samples of 154 patients: 70 with MGUS and 84 with MM were studied. Gene expression was determined by real-time quantitative RT-PCR and TL was evaluated by terminal restriction fragments. A significant overexpression of TRF2, POT1 and hTERT in MM compared to MGUS was observed (p<0.03). Due to the great heterogeneity in hTERT levels, patients were divided into three groups of expression: GI (low), GII (intermediate) and GIII (high). This classification showed an upregulation of TRF2 and RAP1 in MGUS (GIII vs. GI: p<0.01), and an increased expression of TRF2 and TPP1 in MM (GIII vs. GI and GII: p<0.04). In addition, a significant over-expression of LGALS1 (encoding galectin-1, a multifunctional β-galactoside binding protein) was observed in GII/III respect to GI (p=0.013). TL was inversely correlated with telomerase expression (GII vs. GI: p=0.02). In MM, POT1 were positively correlated with β2microglobulin (β2M), advanced DS stages, high calcium levels and bone lesions (p=0.009), whereas TL was correlated positively with hemoglobin levels and negatively with β2M (p=0.02). Our findings provide the first evidence of a global modification in the expression of the six shelterin subunits, their interactions and the correlation between hTERT and LSGAL1 in plasma cell disorders. The strong association of POT1 with different parameters of adverse clinical outcome suggests its importance as a target for novel therapeutic strategies.
Fil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental; Argentina
Fil: Stanganelli, Carmen. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "mariano R. Castex"; Argentina
Fil: Arbelbide, Jorge. Hospital Italiano de Buenos Aires; Argentina
Fil: Fantl, Dorotea Beatriz. Hospital Italiano de Buenos Aires; Argentina
Fil: Kohan, Dana. Hospital Italiano de Buenos Aires; Argentina
Fil: Garcia Rivello, Hernan. Hospital Italiano de Buenos Aires; Argentina
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental; Argentina
description In this study we analyzed the expression profile and the interactions of all shelterin components in patients with MM and MGUS, and correlated the results with hTERT expression, telomere length (TL) and clinical characteristics of the diseases. Bone marrow samples of 154 patients: 70 with MGUS and 84 with MM were studied. Gene expression was determined by real-time quantitative RT-PCR and TL was evaluated by terminal restriction fragments. A significant overexpression of TRF2, POT1 and hTERT in MM compared to MGUS was observed (p<0.03). Due to the great heterogeneity in hTERT levels, patients were divided into three groups of expression: GI (low), GII (intermediate) and GIII (high). This classification showed an upregulation of TRF2 and RAP1 in MGUS (GIII vs. GI: p<0.01), and an increased expression of TRF2 and TPP1 in MM (GIII vs. GI and GII: p<0.04). In addition, a significant over-expression of LGALS1 (encoding galectin-1, a multifunctional β-galactoside binding protein) was observed in GII/III respect to GI (p=0.013). TL was inversely correlated with telomerase expression (GII vs. GI: p=0.02). In MM, POT1 were positively correlated with β2microglobulin (β2M), advanced DS stages, high calcium levels and bone lesions (p=0.009), whereas TL was correlated positively with hemoglobin levels and negatively with β2M (p=0.02). Our findings provide the first evidence of a global modification in the expression of the six shelterin subunits, their interactions and the correlation between hTERT and LSGAL1 in plasma cell disorders. The strong association of POT1 with different parameters of adverse clinical outcome suggests its importance as a target for novel therapeutic strategies.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7635
Panero, Julieta; Stanganelli, Carmen; Arbelbide, Jorge; Fantl, Dorotea Beatriz; Kohan, Dana; et al.; Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression; Elsevier; Blood Cells Molecules And Diseases; 52; 2-3; 2-2014; 134-139
1079-9796
url http://hdl.handle.net/11336/7635
identifier_str_mv Panero, Julieta; Stanganelli, Carmen; Arbelbide, Jorge; Fantl, Dorotea Beatriz; Kohan, Dana; et al.; Expression profile of shelterin components in plasma cell disorders. Clinical significance of POT1 overexpression; Elsevier; Blood Cells Molecules And Diseases; 52; 2-3; 2-2014; 134-139
1079-9796
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1079979613002313
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bcmd.2013.10.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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