Altered mRNA expression of telomere-associated genes in monoclonal gammopathy of undetermined significance and multiple myeloma

Autores
Panero, Julieta; Arbelbide, Jorge; Fantl, Dorotea Beatriz; Garcia Rivello, Hernan Jorge; Kohan, Dana; Slavutsky, Irma Rosa
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, we explored changes in the expression of the telomere maintenance genes, TRF1, TRF2 and TANK1 in patients with monoclonal gammopathy of undetermined significance (MGUS) and multiple myeloma (MM). Results were correlated with human telomerase reverse transcriptase (hTERT) expression, telomere length (TL) and clinicopathological characteristics. Bone marrow (BM) samples from 132 patients, 64 with MGUS and 68 with MM, were studied. Real-time quantitative reverse transcription-polymerase chain reaction was used to quantify gene expression. TL was evaluated by terminal restriction fragment length analysis. MGUS patients showed increased TRF1 levels (P = 0.006) and lower expression of TRF2 (P = 0.005) and TANK1 (P = 0.003) compared with MM patients. For hTERT analysis, patients were divided into three groups by use of receiver operating characteristics: low (group I [GI]), intermediate (group II [GII]) and high (group III [GIII]) expression. We observed increasing expression of TRF2 and TANK1 from GI to GIII in MGUS and MM, with differences for both genes in MM (P < 0.01) and for TRF2 in MGUS (P < 0.01). GIII patients with the highest telomerase expression had the shortest TL. In both entities, a positive association between TRF2-TANK1, TRF2-hTERT and TANK1-hTERT (P ≤ 0.01) was observed. In MM, the percentage of BM infiltration and Ki-67 index were positively associated with TRF2, TANK1 and hTERT expression (P ≤ 0.03) and negatively with TL (P = 0.02), whereas lactate dehydrogenase was significantly correlated with TRF2 mRNA (P = 0.008). Our findings provide the first evidence of a modification in the expression of telomeric proteins in plasma cell disorders, and suggest that mechanisms other than telomerase activation are involved in TL maintenance in these pathologies. © 2010 The Feinstein Institute for Medical Research.
Fil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Arbelbide, Jorge. Hospital Italiano; Argentina
Fil: Fantl, Dorotea Beatriz. Hospital Italiano; Argentina
Fil: Garcia Rivello, Hernan Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; Argentina
Fil: Kohan, Dana. Hospital Italiano; Argentina
Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Materia
Myeloma
MGUS
Telomere
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53289
Acceder
id CONICETDig_1723f9943327a09562cfb94580f87164
oai_identifier_str oai:ri.conicet.gov.ar:11336/53289
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Altered mRNA expression of telomere-associated genes in monoclonal gammopathy of undetermined significance and multiple myelomaPanero, JulietaArbelbide, JorgeFantl, Dorotea BeatrizGarcia Rivello, Hernan JorgeKohan, DanaSlavutsky, Irma RosaMyelomaMGUSTelomerehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In this study, we explored changes in the expression of the telomere maintenance genes, TRF1, TRF2 and TANK1 in patients with monoclonal gammopathy of undetermined significance (MGUS) and multiple myeloma (MM). Results were correlated with human telomerase reverse transcriptase (hTERT) expression, telomere length (TL) and clinicopathological characteristics. Bone marrow (BM) samples from 132 patients, 64 with MGUS and 68 with MM, were studied. Real-time quantitative reverse transcription-polymerase chain reaction was used to quantify gene expression. TL was evaluated by terminal restriction fragment length analysis. MGUS patients showed increased TRF1 levels (P = 0.006) and lower expression of TRF2 (P = 0.005) and TANK1 (P = 0.003) compared with MM patients. For hTERT analysis, patients were divided into three groups by use of receiver operating characteristics: low (group I [GI]), intermediate (group II [GII]) and high (group III [GIII]) expression. We observed increasing expression of TRF2 and TANK1 from GI to GIII in MGUS and MM, with differences for both genes in MM (P < 0.01) and for TRF2 in MGUS (P < 0.01). GIII patients with the highest telomerase expression had the shortest TL. In both entities, a positive association between TRF2-TANK1, TRF2-hTERT and TANK1-hTERT (P ≤ 0.01) was observed. In MM, the percentage of BM infiltration and Ki-67 index were positively associated with TRF2, TANK1 and hTERT expression (P ≤ 0.03) and negatively with TL (P = 0.02), whereas lactate dehydrogenase was significantly correlated with TRF2 mRNA (P = 0.008). Our findings provide the first evidence of a modification in the expression of telomeric proteins in plasma cell disorders, and suggest that mechanisms other than telomerase activation are involved in TL maintenance in these pathologies. © 2010 The Feinstein Institute for Medical Research.Fil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; ArgentinaFil: Arbelbide, Jorge. Hospital Italiano; ArgentinaFil: Fantl, Dorotea Beatriz. Hospital Italiano; ArgentinaFil: Garcia Rivello, Hernan Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; ArgentinaFil: Kohan, Dana. Hospital Italiano; ArgentinaFil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; ArgentinaFeinstein Inst Med Res2010-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53289Panero, Julieta; Arbelbide, Jorge; Fantl, Dorotea Beatriz; Garcia Rivello, Hernan Jorge; Kohan, Dana; et al.; Altered mRNA expression of telomere-associated genes in monoclonal gammopathy of undetermined significance and multiple myeloma; Feinstein Inst Med Res; Molecular Medicine; 16; 11-12; 11-2010; 471-4781528-3658CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2119/molmed.2010.00057info:eu-repo/semantics/altIdentifier/url/http://static.smallworldlabs.com/molmedcommunity/content/pdfstore/10_57_Panero.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:45Zoai:ri.conicet.gov.ar:11336/53289instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:45.852CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Altered mRNA expression of telomere-associated genes in monoclonal gammopathy of undetermined significance and multiple myeloma
title Altered mRNA expression of telomere-associated genes in monoclonal gammopathy of undetermined significance and multiple myeloma
spellingShingle Altered mRNA expression of telomere-associated genes in monoclonal gammopathy of undetermined significance and multiple myeloma
Panero, Julieta
Myeloma
MGUS
Telomere
title_short Altered mRNA expression of telomere-associated genes in monoclonal gammopathy of undetermined significance and multiple myeloma
title_full Altered mRNA expression of telomere-associated genes in monoclonal gammopathy of undetermined significance and multiple myeloma
title_fullStr Altered mRNA expression of telomere-associated genes in monoclonal gammopathy of undetermined significance and multiple myeloma
title_full_unstemmed Altered mRNA expression of telomere-associated genes in monoclonal gammopathy of undetermined significance and multiple myeloma
title_sort Altered mRNA expression of telomere-associated genes in monoclonal gammopathy of undetermined significance and multiple myeloma
dc.creator.none.fl_str_mv Panero, Julieta
Arbelbide, Jorge
Fantl, Dorotea Beatriz
Garcia Rivello, Hernan Jorge
Kohan, Dana
Slavutsky, Irma Rosa
author Panero, Julieta
author_facet Panero, Julieta
Arbelbide, Jorge
Fantl, Dorotea Beatriz
Garcia Rivello, Hernan Jorge
Kohan, Dana
Slavutsky, Irma Rosa
author_role author
author2 Arbelbide, Jorge
Fantl, Dorotea Beatriz
Garcia Rivello, Hernan Jorge
Kohan, Dana
Slavutsky, Irma Rosa
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Myeloma
MGUS
Telomere
topic Myeloma
MGUS
Telomere
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In this study, we explored changes in the expression of the telomere maintenance genes, TRF1, TRF2 and TANK1 in patients with monoclonal gammopathy of undetermined significance (MGUS) and multiple myeloma (MM). Results were correlated with human telomerase reverse transcriptase (hTERT) expression, telomere length (TL) and clinicopathological characteristics. Bone marrow (BM) samples from 132 patients, 64 with MGUS and 68 with MM, were studied. Real-time quantitative reverse transcription-polymerase chain reaction was used to quantify gene expression. TL was evaluated by terminal restriction fragment length analysis. MGUS patients showed increased TRF1 levels (P = 0.006) and lower expression of TRF2 (P = 0.005) and TANK1 (P = 0.003) compared with MM patients. For hTERT analysis, patients were divided into three groups by use of receiver operating characteristics: low (group I [GI]), intermediate (group II [GII]) and high (group III [GIII]) expression. We observed increasing expression of TRF2 and TANK1 from GI to GIII in MGUS and MM, with differences for both genes in MM (P < 0.01) and for TRF2 in MGUS (P < 0.01). GIII patients with the highest telomerase expression had the shortest TL. In both entities, a positive association between TRF2-TANK1, TRF2-hTERT and TANK1-hTERT (P ≤ 0.01) was observed. In MM, the percentage of BM infiltration and Ki-67 index were positively associated with TRF2, TANK1 and hTERT expression (P ≤ 0.03) and negatively with TL (P = 0.02), whereas lactate dehydrogenase was significantly correlated with TRF2 mRNA (P = 0.008). Our findings provide the first evidence of a modification in the expression of telomeric proteins in plasma cell disorders, and suggest that mechanisms other than telomerase activation are involved in TL maintenance in these pathologies. © 2010 The Feinstein Institute for Medical Research.
Fil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
Fil: Arbelbide, Jorge. Hospital Italiano; Argentina
Fil: Fantl, Dorotea Beatriz. Hospital Italiano; Argentina
Fil: Garcia Rivello, Hernan Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; Argentina
Fil: Kohan, Dana. Hospital Italiano; Argentina
Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
description In this study, we explored changes in the expression of the telomere maintenance genes, TRF1, TRF2 and TANK1 in patients with monoclonal gammopathy of undetermined significance (MGUS) and multiple myeloma (MM). Results were correlated with human telomerase reverse transcriptase (hTERT) expression, telomere length (TL) and clinicopathological characteristics. Bone marrow (BM) samples from 132 patients, 64 with MGUS and 68 with MM, were studied. Real-time quantitative reverse transcription-polymerase chain reaction was used to quantify gene expression. TL was evaluated by terminal restriction fragment length analysis. MGUS patients showed increased TRF1 levels (P = 0.006) and lower expression of TRF2 (P = 0.005) and TANK1 (P = 0.003) compared with MM patients. For hTERT analysis, patients were divided into three groups by use of receiver operating characteristics: low (group I [GI]), intermediate (group II [GII]) and high (group III [GIII]) expression. We observed increasing expression of TRF2 and TANK1 from GI to GIII in MGUS and MM, with differences for both genes in MM (P < 0.01) and for TRF2 in MGUS (P < 0.01). GIII patients with the highest telomerase expression had the shortest TL. In both entities, a positive association between TRF2-TANK1, TRF2-hTERT and TANK1-hTERT (P ≤ 0.01) was observed. In MM, the percentage of BM infiltration and Ki-67 index were positively associated with TRF2, TANK1 and hTERT expression (P ≤ 0.03) and negatively with TL (P = 0.02), whereas lactate dehydrogenase was significantly correlated with TRF2 mRNA (P = 0.008). Our findings provide the first evidence of a modification in the expression of telomeric proteins in plasma cell disorders, and suggest that mechanisms other than telomerase activation are involved in TL maintenance in these pathologies. © 2010 The Feinstein Institute for Medical Research.
publishDate 2010
dc.date.none.fl_str_mv 2010-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53289
Panero, Julieta; Arbelbide, Jorge; Fantl, Dorotea Beatriz; Garcia Rivello, Hernan Jorge; Kohan, Dana; et al.; Altered mRNA expression of telomere-associated genes in monoclonal gammopathy of undetermined significance and multiple myeloma; Feinstein Inst Med Res; Molecular Medicine; 16; 11-12; 11-2010; 471-478
1528-3658
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53289
identifier_str_mv Panero, Julieta; Arbelbide, Jorge; Fantl, Dorotea Beatriz; Garcia Rivello, Hernan Jorge; Kohan, Dana; et al.; Altered mRNA expression of telomere-associated genes in monoclonal gammopathy of undetermined significance and multiple myeloma; Feinstein Inst Med Res; Molecular Medicine; 16; 11-12; 11-2010; 471-478
1528-3658
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.2119/molmed.2010.00057
info:eu-repo/semantics/altIdentifier/url/http://static.smallworldlabs.com/molmedcommunity/content/pdfstore/10_57_Panero.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Feinstein Inst Med Res
publisher.none.fl_str_mv Feinstein Inst Med Res
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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