Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders

Autores
Panero, Julieta; O'Callaghan, Nathan J.; Fenech, Michael; Slavutsky, Irma Rosa
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Telomere length (TL) is currently used as an emerging biomarker in understanding the development/progression of hematological malignancies. The absolute quantitative PCR (qPCR) methodology has allowed the study of TL from a variety of mammalian tissues, but it has not been tested for bone marrow (BM) samples. In this study, we have examined the relationship between TL data generated by absolute qPCR versus those obtained by terminal restriction fragments (TRF) in 102 BM samples from patients with plasma cell disorders. A significant linear correlation between both methodologies was observed (p < 0.0001; r2 = 0.70). Results were also analyzed in relation to clinical characteristics and significant associations between telomere shortening and parameters of adverse prognosis were observed. Furthermore, another set of 47 BM samples from patients with low quantity of DNA for TRF assay were suitably analyzed by qPCR, indicating the usefulness of the absolute qPCR methodology for the inclusion of patients with scarce material to the study. Taken together, these findings are of interest considering the importance of telomere dysfunction in the pathogenesis of cancer and give a new alternative to measure TL in hematologic disorders with substantial time and cost savings.
Fil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: O'Callaghan, Nathan J.. Commonwealth Scientific and Industrial Research Organization; Australia
Fil: Fenech, Michael. Commonwealth Scientific and Industrial Research Organization; Australia
Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Materia
Absolute Quantification
Bone Marrow Samples
Mgus
Multiple Myeloma
Telomere Length
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/38264
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/38264
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell DisordersPanero, JulietaO'Callaghan, Nathan J.Fenech, MichaelSlavutsky, Irma RosaAbsolute QuantificationBone Marrow SamplesMgusMultiple MyelomaTelomere Lengthhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Telomere length (TL) is currently used as an emerging biomarker in understanding the development/progression of hematological malignancies. The absolute quantitative PCR (qPCR) methodology has allowed the study of TL from a variety of mammalian tissues, but it has not been tested for bone marrow (BM) samples. In this study, we have examined the relationship between TL data generated by absolute qPCR versus those obtained by terminal restriction fragments (TRF) in 102 BM samples from patients with plasma cell disorders. A significant linear correlation between both methodologies was observed (p < 0.0001; r2 = 0.70). Results were also analyzed in relation to clinical characteristics and significant associations between telomere shortening and parameters of adverse prognosis were observed. Furthermore, another set of 47 BM samples from patients with low quantity of DNA for TRF assay were suitably analyzed by qPCR, indicating the usefulness of the absolute qPCR methodology for the inclusion of patients with scarce material to the study. Taken together, these findings are of interest considering the importance of telomere dysfunction in the pathogenesis of cancer and give a new alternative to measure TL in hematologic disorders with substantial time and cost savings.Fil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: O'Callaghan, Nathan J.. Commonwealth Scientific and Industrial Research Organization; AustraliaFil: Fenech, Michael. Commonwealth Scientific and Industrial Research Organization; AustraliaFil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaHumana Press2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38264Panero, Julieta; O'Callaghan, Nathan J.; Fenech, Michael; Slavutsky, Irma Rosa; Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders; Humana Press; Molecular Biotechnology; 57; 2; 2-2015; 155-1591073-6085CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s12033-014-9811-8info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12033-014-9811-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:02Zoai:ri.conicet.gov.ar:11336/38264instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:02.884CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders
title Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders
spellingShingle Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders
Panero, Julieta
Absolute Quantification
Bone Marrow Samples
Mgus
Multiple Myeloma
Telomere Length
title_short Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders
title_full Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders
title_fullStr Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders
title_full_unstemmed Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders
title_sort Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders
dc.creator.none.fl_str_mv Panero, Julieta
O'Callaghan, Nathan J.
Fenech, Michael
Slavutsky, Irma Rosa
author Panero, Julieta
author_facet Panero, Julieta
O'Callaghan, Nathan J.
Fenech, Michael
Slavutsky, Irma Rosa
author_role author
author2 O'Callaghan, Nathan J.
Fenech, Michael
Slavutsky, Irma Rosa
author2_role author
author
author
dc.subject.none.fl_str_mv Absolute Quantification
Bone Marrow Samples
Mgus
Multiple Myeloma
Telomere Length
topic Absolute Quantification
Bone Marrow Samples
Mgus
Multiple Myeloma
Telomere Length
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Telomere length (TL) is currently used as an emerging biomarker in understanding the development/progression of hematological malignancies. The absolute quantitative PCR (qPCR) methodology has allowed the study of TL from a variety of mammalian tissues, but it has not been tested for bone marrow (BM) samples. In this study, we have examined the relationship between TL data generated by absolute qPCR versus those obtained by terminal restriction fragments (TRF) in 102 BM samples from patients with plasma cell disorders. A significant linear correlation between both methodologies was observed (p < 0.0001; r2 = 0.70). Results were also analyzed in relation to clinical characteristics and significant associations between telomere shortening and parameters of adverse prognosis were observed. Furthermore, another set of 47 BM samples from patients with low quantity of DNA for TRF assay were suitably analyzed by qPCR, indicating the usefulness of the absolute qPCR methodology for the inclusion of patients with scarce material to the study. Taken together, these findings are of interest considering the importance of telomere dysfunction in the pathogenesis of cancer and give a new alternative to measure TL in hematologic disorders with substantial time and cost savings.
Fil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: O'Callaghan, Nathan J.. Commonwealth Scientific and Industrial Research Organization; Australia
Fil: Fenech, Michael. Commonwealth Scientific and Industrial Research Organization; Australia
Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
description Telomere length (TL) is currently used as an emerging biomarker in understanding the development/progression of hematological malignancies. The absolute quantitative PCR (qPCR) methodology has allowed the study of TL from a variety of mammalian tissues, but it has not been tested for bone marrow (BM) samples. In this study, we have examined the relationship between TL data generated by absolute qPCR versus those obtained by terminal restriction fragments (TRF) in 102 BM samples from patients with plasma cell disorders. A significant linear correlation between both methodologies was observed (p < 0.0001; r2 = 0.70). Results were also analyzed in relation to clinical characteristics and significant associations between telomere shortening and parameters of adverse prognosis were observed. Furthermore, another set of 47 BM samples from patients with low quantity of DNA for TRF assay were suitably analyzed by qPCR, indicating the usefulness of the absolute qPCR methodology for the inclusion of patients with scarce material to the study. Taken together, these findings are of interest considering the importance of telomere dysfunction in the pathogenesis of cancer and give a new alternative to measure TL in hematologic disorders with substantial time and cost savings.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38264
Panero, Julieta; O'Callaghan, Nathan J.; Fenech, Michael; Slavutsky, Irma Rosa; Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders; Humana Press; Molecular Biotechnology; 57; 2; 2-2015; 155-159
1073-6085
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38264
identifier_str_mv Panero, Julieta; O'Callaghan, Nathan J.; Fenech, Michael; Slavutsky, Irma Rosa; Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders; Humana Press; Molecular Biotechnology; 57; 2; 2-2015; 155-159
1073-6085
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s12033-014-9811-8
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12033-014-9811-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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