Quantitative analysis of CKS1B mRNA expression and copy number gain in patients with plasma cell disorders

Autores
Stella, Flavia; Pedrazzini, Estela Marta; Baialardo, Edgardo; Fantl, Dorotea; Schutz, Natalia; Slavutsky, Irma Rosa
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, we have examined CKS1B gene expression and copy number in a total of 114- patients at diagnosis: 83 with multiple myeloma (MM) and 31 with monoclonal gammopathy of undetermined significance (MGUS). Results were correlated with cytogenetics, FISH and clinical characteristic. Significant CKS1B mRNA levels in MM compared to MGUS cases (p < 0.048) were detected. In MM, the frequency of 1q21 (CKS1B) copy gain was significantly higher in cases with abnormal karyotype compared to patients with normal karyotype (p = 0.021). Global analysis showed a positive correlation between CKS1B expression and 1q21 copy number (p < 0.0001). No association between CKS1B mRNA expression and clinical parameters was found. However, a significantly higher level of β2 microglobulin in cases with 1q21 gains than those without (p = 0.0094) was observed. Overall survival was shorter in cases with 1q21 gain compared to those with normal 1q21 region (p = 0.0082). Our results suggest a role for CKS1B in the multiple step process of progression of MGUS to MM and show that CKS1B copy gain has a more significant prognostic value than its overexpression. This adverse impact on survival probably reflects the genetic instability associated to chromosome 1q alterations resulting in a more aggressive behavior of the disease.
Fil: Stella, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Pedrazzini, Estela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Baialardo, Edgardo. Centro de Estudios Genéticos. Buenos Aires; Argentina
Fil: Fantl, Dorotea. Hospital Italiano; Argentina
Fil: Schutz, Natalia. Hospital Italiano; Argentina
Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Materia
Multiple Myeloma
Mgus
Cks1b Gene Expression
Fish
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/35930
Acceder
id CONICETDig_0853a7f590d6b3a114c1886917ccac56
oai_identifier_str oai:ri.conicet.gov.ar:11336/35930
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Quantitative analysis of CKS1B mRNA expression and copy number gain in patients with plasma cell disordersStella, FlaviaPedrazzini, Estela MartaBaialardo, EdgardoFantl, DoroteaSchutz, NataliaSlavutsky, Irma RosaMultiple MyelomaMgusCks1b Gene ExpressionFishhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3In this study, we have examined CKS1B gene expression and copy number in a total of 114- patients at diagnosis: 83 with multiple myeloma (MM) and 31 with monoclonal gammopathy of undetermined significance (MGUS). Results were correlated with cytogenetics, FISH and clinical characteristic. Significant CKS1B mRNA levels in MM compared to MGUS cases (p < 0.048) were detected. In MM, the frequency of 1q21 (CKS1B) copy gain was significantly higher in cases with abnormal karyotype compared to patients with normal karyotype (p = 0.021). Global analysis showed a positive correlation between CKS1B expression and 1q21 copy number (p < 0.0001). No association between CKS1B mRNA expression and clinical parameters was found. However, a significantly higher level of β2 microglobulin in cases with 1q21 gains than those without (p = 0.0094) was observed. Overall survival was shorter in cases with 1q21 gain compared to those with normal 1q21 region (p = 0.0082). Our results suggest a role for CKS1B in the multiple step process of progression of MGUS to MM and show that CKS1B copy gain has a more significant prognostic value than its overexpression. This adverse impact on survival probably reflects the genetic instability associated to chromosome 1q alterations resulting in a more aggressive behavior of the disease.Fil: Stella, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Pedrazzini, Estela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; ArgentinaFil: Baialardo, Edgardo. Centro de Estudios Genéticos. Buenos Aires; ArgentinaFil: Fantl, Dorotea. Hospital Italiano; ArgentinaFil: Schutz, Natalia. Hospital Italiano; ArgentinaFil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaAcademic Press Inc Elsevier Science2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/35930Stella, Flavia; Pedrazzini, Estela Marta; Baialardo, Edgardo; Fantl, Dorotea; Schutz, Natalia; et al.; Quantitative analysis of CKS1B mRNA expression and copy number gain in patients with plasma cell disorders; Academic Press Inc Elsevier Science; Blood Cells Molecules And Diseases; 53; 3; 9-2014; 110-1171079-9796CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bcmd.2014.05.006info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1079979614000576info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:37:58Zoai:ri.conicet.gov.ar:11336/35930instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:37:58.612CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Quantitative analysis of CKS1B mRNA expression and copy number gain in patients with plasma cell disorders
title Quantitative analysis of CKS1B mRNA expression and copy number gain in patients with plasma cell disorders
spellingShingle Quantitative analysis of CKS1B mRNA expression and copy number gain in patients with plasma cell disorders
Stella, Flavia
Multiple Myeloma
Mgus
Cks1b Gene Expression
Fish
title_short Quantitative analysis of CKS1B mRNA expression and copy number gain in patients with plasma cell disorders
title_full Quantitative analysis of CKS1B mRNA expression and copy number gain in patients with plasma cell disorders
title_fullStr Quantitative analysis of CKS1B mRNA expression and copy number gain in patients with plasma cell disorders
title_full_unstemmed Quantitative analysis of CKS1B mRNA expression and copy number gain in patients with plasma cell disorders
title_sort Quantitative analysis of CKS1B mRNA expression and copy number gain in patients with plasma cell disorders
dc.creator.none.fl_str_mv Stella, Flavia
Pedrazzini, Estela Marta
Baialardo, Edgardo
Fantl, Dorotea
Schutz, Natalia
Slavutsky, Irma Rosa
author Stella, Flavia
author_facet Stella, Flavia
Pedrazzini, Estela Marta
Baialardo, Edgardo
Fantl, Dorotea
Schutz, Natalia
Slavutsky, Irma Rosa
author_role author
author2 Pedrazzini, Estela Marta
Baialardo, Edgardo
Fantl, Dorotea
Schutz, Natalia
Slavutsky, Irma Rosa
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Multiple Myeloma
Mgus
Cks1b Gene Expression
Fish
topic Multiple Myeloma
Mgus
Cks1b Gene Expression
Fish
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In this study, we have examined CKS1B gene expression and copy number in a total of 114- patients at diagnosis: 83 with multiple myeloma (MM) and 31 with monoclonal gammopathy of undetermined significance (MGUS). Results were correlated with cytogenetics, FISH and clinical characteristic. Significant CKS1B mRNA levels in MM compared to MGUS cases (p < 0.048) were detected. In MM, the frequency of 1q21 (CKS1B) copy gain was significantly higher in cases with abnormal karyotype compared to patients with normal karyotype (p = 0.021). Global analysis showed a positive correlation between CKS1B expression and 1q21 copy number (p < 0.0001). No association between CKS1B mRNA expression and clinical parameters was found. However, a significantly higher level of β2 microglobulin in cases with 1q21 gains than those without (p = 0.0094) was observed. Overall survival was shorter in cases with 1q21 gain compared to those with normal 1q21 region (p = 0.0082). Our results suggest a role for CKS1B in the multiple step process of progression of MGUS to MM and show that CKS1B copy gain has a more significant prognostic value than its overexpression. This adverse impact on survival probably reflects the genetic instability associated to chromosome 1q alterations resulting in a more aggressive behavior of the disease.
Fil: Stella, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Pedrazzini, Estela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Baialardo, Edgardo. Centro de Estudios Genéticos. Buenos Aires; Argentina
Fil: Fantl, Dorotea. Hospital Italiano; Argentina
Fil: Schutz, Natalia. Hospital Italiano; Argentina
Fil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
description In this study, we have examined CKS1B gene expression and copy number in a total of 114- patients at diagnosis: 83 with multiple myeloma (MM) and 31 with monoclonal gammopathy of undetermined significance (MGUS). Results were correlated with cytogenetics, FISH and clinical characteristic. Significant CKS1B mRNA levels in MM compared to MGUS cases (p < 0.048) were detected. In MM, the frequency of 1q21 (CKS1B) copy gain was significantly higher in cases with abnormal karyotype compared to patients with normal karyotype (p = 0.021). Global analysis showed a positive correlation between CKS1B expression and 1q21 copy number (p < 0.0001). No association between CKS1B mRNA expression and clinical parameters was found. However, a significantly higher level of β2 microglobulin in cases with 1q21 gains than those without (p = 0.0094) was observed. Overall survival was shorter in cases with 1q21 gain compared to those with normal 1q21 region (p = 0.0082). Our results suggest a role for CKS1B in the multiple step process of progression of MGUS to MM and show that CKS1B copy gain has a more significant prognostic value than its overexpression. This adverse impact on survival probably reflects the genetic instability associated to chromosome 1q alterations resulting in a more aggressive behavior of the disease.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/35930
Stella, Flavia; Pedrazzini, Estela Marta; Baialardo, Edgardo; Fantl, Dorotea; Schutz, Natalia; et al.; Quantitative analysis of CKS1B mRNA expression and copy number gain in patients with plasma cell disorders; Academic Press Inc Elsevier Science; Blood Cells Molecules And Diseases; 53; 3; 9-2014; 110-117
1079-9796
CONICET Digital
CONICET
url http://hdl.handle.net/11336/35930
identifier_str_mv Stella, Flavia; Pedrazzini, Estela Marta; Baialardo, Edgardo; Fantl, Dorotea; Schutz, Natalia; et al.; Quantitative analysis of CKS1B mRNA expression and copy number gain in patients with plasma cell disorders; Academic Press Inc Elsevier Science; Blood Cells Molecules And Diseases; 53; 3; 9-2014; 110-117
1079-9796
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bcmd.2014.05.006
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1079979614000576
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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