Mitochondrial Dysfunction and Tissue Alterations of Ultraviolet-Irradiated Skin in Five Different Mice Strains

Autores
Friedrich, Adrian; Paz, Mariela Laura; Cela, Eliana Maiten; Leoni, Juliana; Gonzalez Maglio, Daniel Horacio
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ultraviolet radiation (UVR) effects on skin have been extensively studied. Several mice strains have been used worldwide in photobiology and photoimmunology studies. Recently, we have developed a method based on flow cytometry in order to analyze mitochondrial dysfunction and superoxide (O2•–) production ex vivo in keratinocytes isolated from irradiated-mice. This method can be helpful to evaluate mitochondrial alterations in different mice models. In this work, we aimed to compare epidermal response to UVR in five mice strains, both pigmented and albino as well as hairy and hairless strains (SKH:1, Balb/c, C57/BL, DBA/2N and Swiss). Keratinocytes mitochondrial alterations, epidermal hyperplasia and inflammatory mediators’ production (epidermis and serum) were determined 72 hours after a 400 mJ/cm2 UV dose. All strains showed epidermal hyperplasia and loss of mitochondrial polarization after irradiation, differing in the magnitude of the response. However, there were significant differences in the basal mitochondrial polarization between strains, showing that the metabolic state of keratinocytes may vary between them. Moreover, mitochondrial O2•– production was induced in SKH:1 and Balb/c after irradiation, whereas in DBA/2N, Swiss and C57/BL it was at the same level or even lower than in the non-irradiated control. Finally, an increase in inflammatory mediators was only detected in the serum of C57/BL and Swiss mice and in the epidermis of DBA/2N and C57/BL. Results show that each mice strain has particular characteristics related to cellular metabolism, which may lead to particular responses to UVR exposure. Therefore, the use of a particular mice strain in photobiology models should be carefully considered
Fil: Friedrich, Adrian. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina
Fil: Paz, Mariela Laura. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina
Fil: Cela, Eliana Maiten. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Leoni, Juliana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina
Fil: Gonzalez Maglio, Daniel Horacio. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Materia
Mitochondrial Function
Inflammation
Uv Irradiation
Epidermis
Keratinocytes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8036
Acceder
id CONICETDig_b8a93e84066c0c7e101da0e7a3773bcd
oai_identifier_str oai:ri.conicet.gov.ar:11336/8036
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mitochondrial Dysfunction and Tissue Alterations of Ultraviolet-Irradiated Skin in Five Different Mice StrainsFriedrich, AdrianPaz, Mariela LauraCela, Eliana MaitenLeoni, JulianaGonzalez Maglio, Daniel HoracioMitochondrial FunctionInflammationUv IrradiationEpidermisKeratinocyteshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Ultraviolet radiation (UVR) effects on skin have been extensively studied. Several mice strains have been used worldwide in photobiology and photoimmunology studies. Recently, we have developed a method based on flow cytometry in order to analyze mitochondrial dysfunction and superoxide (O2•–) production ex vivo in keratinocytes isolated from irradiated-mice. This method can be helpful to evaluate mitochondrial alterations in different mice models. In this work, we aimed to compare epidermal response to UVR in five mice strains, both pigmented and albino as well as hairy and hairless strains (SKH:1, Balb/c, C57/BL, DBA/2N and Swiss). Keratinocytes mitochondrial alterations, epidermal hyperplasia and inflammatory mediators’ production (epidermis and serum) were determined 72 hours after a 400 mJ/cm2 UV dose. All strains showed epidermal hyperplasia and loss of mitochondrial polarization after irradiation, differing in the magnitude of the response. However, there were significant differences in the basal mitochondrial polarization between strains, showing that the metabolic state of keratinocytes may vary between them. Moreover, mitochondrial O2•– production was induced in SKH:1 and Balb/c after irradiation, whereas in DBA/2N, Swiss and C57/BL it was at the same level or even lower than in the non-irradiated control. Finally, an increase in inflammatory mediators was only detected in the serum of C57/BL and Swiss mice and in the epidermis of DBA/2N and C57/BL. Results show that each mice strain has particular characteristics related to cellular metabolism, which may lead to particular responses to UVR exposure. Therefore, the use of a particular mice strain in photobiology models should be carefully consideredFil: Friedrich, Adrian. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; ArgentinaFil: Paz, Mariela Laura. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; ArgentinaFil: Cela, Eliana Maiten. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; ArgentinaFil: Leoni, Juliana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; ArgentinaFil: Gonzalez Maglio, Daniel Horacio. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; ArgentinaSynergy Publishers2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8036Friedrich, Adrian; Paz, Mariela Laura; Cela, Eliana Maiten; Leoni, Juliana; Gonzalez Maglio, Daniel Horacio; Mitochondrial Dysfunction and Tissue Alterations of Ultraviolet-Irradiated Skin in Five Different Mice Strains; Synergy Publishers; Global Journal of Dermatology and Venereology; 2; 1; 2-2014; 4-122310-998Xenginfo:eu-repo/semantics/altIdentifier/url/http://www.synergypublishers.com/downloads/gjdvv2n1a2/info:eu-repo/semantics/altIdentifier/doi/10.12970/2310-998X.2014.02.01.2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:54Zoai:ri.conicet.gov.ar:11336/8036instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:54.647CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mitochondrial Dysfunction and Tissue Alterations of Ultraviolet-Irradiated Skin in Five Different Mice Strains
title Mitochondrial Dysfunction and Tissue Alterations of Ultraviolet-Irradiated Skin in Five Different Mice Strains
spellingShingle Mitochondrial Dysfunction and Tissue Alterations of Ultraviolet-Irradiated Skin in Five Different Mice Strains
Friedrich, Adrian
Mitochondrial Function
Inflammation
Uv Irradiation
Epidermis
Keratinocytes
title_short Mitochondrial Dysfunction and Tissue Alterations of Ultraviolet-Irradiated Skin in Five Different Mice Strains
title_full Mitochondrial Dysfunction and Tissue Alterations of Ultraviolet-Irradiated Skin in Five Different Mice Strains
title_fullStr Mitochondrial Dysfunction and Tissue Alterations of Ultraviolet-Irradiated Skin in Five Different Mice Strains
title_full_unstemmed Mitochondrial Dysfunction and Tissue Alterations of Ultraviolet-Irradiated Skin in Five Different Mice Strains
title_sort Mitochondrial Dysfunction and Tissue Alterations of Ultraviolet-Irradiated Skin in Five Different Mice Strains
dc.creator.none.fl_str_mv Friedrich, Adrian
Paz, Mariela Laura
Cela, Eliana Maiten
Leoni, Juliana
Gonzalez Maglio, Daniel Horacio
author Friedrich, Adrian
author_facet Friedrich, Adrian
Paz, Mariela Laura
Cela, Eliana Maiten
Leoni, Juliana
Gonzalez Maglio, Daniel Horacio
author_role author
author2 Paz, Mariela Laura
Cela, Eliana Maiten
Leoni, Juliana
Gonzalez Maglio, Daniel Horacio
author2_role author
author
author
author
dc.subject.none.fl_str_mv Mitochondrial Function
Inflammation
Uv Irradiation
Epidermis
Keratinocytes
topic Mitochondrial Function
Inflammation
Uv Irradiation
Epidermis
Keratinocytes
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Ultraviolet radiation (UVR) effects on skin have been extensively studied. Several mice strains have been used worldwide in photobiology and photoimmunology studies. Recently, we have developed a method based on flow cytometry in order to analyze mitochondrial dysfunction and superoxide (O2•–) production ex vivo in keratinocytes isolated from irradiated-mice. This method can be helpful to evaluate mitochondrial alterations in different mice models. In this work, we aimed to compare epidermal response to UVR in five mice strains, both pigmented and albino as well as hairy and hairless strains (SKH:1, Balb/c, C57/BL, DBA/2N and Swiss). Keratinocytes mitochondrial alterations, epidermal hyperplasia and inflammatory mediators’ production (epidermis and serum) were determined 72 hours after a 400 mJ/cm2 UV dose. All strains showed epidermal hyperplasia and loss of mitochondrial polarization after irradiation, differing in the magnitude of the response. However, there were significant differences in the basal mitochondrial polarization between strains, showing that the metabolic state of keratinocytes may vary between them. Moreover, mitochondrial O2•– production was induced in SKH:1 and Balb/c after irradiation, whereas in DBA/2N, Swiss and C57/BL it was at the same level or even lower than in the non-irradiated control. Finally, an increase in inflammatory mediators was only detected in the serum of C57/BL and Swiss mice and in the epidermis of DBA/2N and C57/BL. Results show that each mice strain has particular characteristics related to cellular metabolism, which may lead to particular responses to UVR exposure. Therefore, the use of a particular mice strain in photobiology models should be carefully considered
Fil: Friedrich, Adrian. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina
Fil: Paz, Mariela Laura. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina
Fil: Cela, Eliana Maiten. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Leoni, Juliana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina
Fil: Gonzalez Maglio, Daniel Horacio. Consejo Nacional de Investigaciones Cientiâ­ficas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
description Ultraviolet radiation (UVR) effects on skin have been extensively studied. Several mice strains have been used worldwide in photobiology and photoimmunology studies. Recently, we have developed a method based on flow cytometry in order to analyze mitochondrial dysfunction and superoxide (O2•–) production ex vivo in keratinocytes isolated from irradiated-mice. This method can be helpful to evaluate mitochondrial alterations in different mice models. In this work, we aimed to compare epidermal response to UVR in five mice strains, both pigmented and albino as well as hairy and hairless strains (SKH:1, Balb/c, C57/BL, DBA/2N and Swiss). Keratinocytes mitochondrial alterations, epidermal hyperplasia and inflammatory mediators’ production (epidermis and serum) were determined 72 hours after a 400 mJ/cm2 UV dose. All strains showed epidermal hyperplasia and loss of mitochondrial polarization after irradiation, differing in the magnitude of the response. However, there were significant differences in the basal mitochondrial polarization between strains, showing that the metabolic state of keratinocytes may vary between them. Moreover, mitochondrial O2•– production was induced in SKH:1 and Balb/c after irradiation, whereas in DBA/2N, Swiss and C57/BL it was at the same level or even lower than in the non-irradiated control. Finally, an increase in inflammatory mediators was only detected in the serum of C57/BL and Swiss mice and in the epidermis of DBA/2N and C57/BL. Results show that each mice strain has particular characteristics related to cellular metabolism, which may lead to particular responses to UVR exposure. Therefore, the use of a particular mice strain in photobiology models should be carefully considered
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8036
Friedrich, Adrian; Paz, Mariela Laura; Cela, Eliana Maiten; Leoni, Juliana; Gonzalez Maglio, Daniel Horacio; Mitochondrial Dysfunction and Tissue Alterations of Ultraviolet-Irradiated Skin in Five Different Mice Strains; Synergy Publishers; Global Journal of Dermatology and Venereology; 2; 1; 2-2014; 4-12
2310-998X
url http://hdl.handle.net/11336/8036
identifier_str_mv Friedrich, Adrian; Paz, Mariela Laura; Cela, Eliana Maiten; Leoni, Juliana; Gonzalez Maglio, Daniel Horacio; Mitochondrial Dysfunction and Tissue Alterations of Ultraviolet-Irradiated Skin in Five Different Mice Strains; Synergy Publishers; Global Journal of Dermatology and Venereology; 2; 1; 2-2014; 4-12
2310-998X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.synergypublishers.com/downloads/gjdvv2n1a2/
info:eu-repo/semantics/altIdentifier/doi/10.12970/2310-998X.2014.02.01.2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Synergy Publishers
publisher.none.fl_str_mv Synergy Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614354927878144
score 13.070432

Publicaciones similares