Changes in cell wall polysaccharides and cell wall degrading enzymes during ripening of Fragaria chiloensis and Fragaria ×ananassa fruits

Autores
Figueroa, Carlos R.; Rosli, Hernan Guillermo; Civello, Pedro Marcos; Martinez, Gustavo Adolfo; Herrera, Raúl; Moya León, María A.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chilean strawberry (Fragaria chiloensis) is arising as a new fruit crop that can diversify the world supply of berries. Fruit softening is thought to be linked to an extensive modification of pectin and hemicellulosic cell wall fractions which in turn is determined to a great extent by the action of some cell wall-modifying enzymes. The objective of this work was to compare cell wall changes and the enzyme activities of pectin methylesterase (PME), polygalacturonase (PG), endoglucanase (EGase), α-arabinofuranosidase (AFase), β-galactosidase (βGal), and β-xylosidase (βXyl) between F. chiloensis and Fragaria ×ananassa (cv. Chandler) at three fruit developmental stages: large green (LG), turning (T) and ripe (R). A rapid decrease in fruit firmness between LG and T stages was observed in both species; nevertheless firmness reduction in F. chiloensis was steeper than in F. ×ananassa. This could be related to a faster loss of HCl-soluble polymers (HSP fraction) presented in F. chiloensis fruit. However, commercial F. ×ananassa showed a greater depolymerization of HSP polymers than F. chiloensis. With the exception of PME, the activity of all assayed enzymes was higher in F. chiloensis than in F. ×ananassa fruits. The role of PG and EGase seems to be central in both species: these enzymes are correlated to the decrease in the content of HSP and NaOH-soluble polymers (NSP fraction), respectively. In addition, activity levels of βGal and βXyl were significantly correlated with fruit firmness reduction in F. chiloensis and F. ×ananassa, respectively. © 2010 Elsevier B.V. All rights reserved.
Fil: Figueroa, Carlos R.. Universidad de Talca; Chile
Fil: Rosli, Hernan Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Herrera, Raúl. Universidad de Talca; Chile
Fil: Moya León, María A.. Universidad de Talca; Chile
Materia
CELL WALL POLYMERS
CELL WALL-MODIFYING ENZYMES
FRUIT SOFTENING
STRAWBERRY SPECIES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/152164
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/152164
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Changes in cell wall polysaccharides and cell wall degrading enzymes during ripening of Fragaria chiloensis and Fragaria ×ananassa fruitsFigueroa, Carlos R.Rosli, Hernan GuillermoCivello, Pedro MarcosMartinez, Gustavo AdolfoHerrera, RaúlMoya León, María A.CELL WALL POLYMERSCELL WALL-MODIFYING ENZYMESFRUIT SOFTENINGSTRAWBERRY SPECIEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Chilean strawberry (Fragaria chiloensis) is arising as a new fruit crop that can diversify the world supply of berries. Fruit softening is thought to be linked to an extensive modification of pectin and hemicellulosic cell wall fractions which in turn is determined to a great extent by the action of some cell wall-modifying enzymes. The objective of this work was to compare cell wall changes and the enzyme activities of pectin methylesterase (PME), polygalacturonase (PG), endoglucanase (EGase), α-arabinofuranosidase (AFase), β-galactosidase (βGal), and β-xylosidase (βXyl) between F. chiloensis and Fragaria ×ananassa (cv. Chandler) at three fruit developmental stages: large green (LG), turning (T) and ripe (R). A rapid decrease in fruit firmness between LG and T stages was observed in both species; nevertheless firmness reduction in F. chiloensis was steeper than in F. ×ananassa. This could be related to a faster loss of HCl-soluble polymers (HSP fraction) presented in F. chiloensis fruit. However, commercial F. ×ananassa showed a greater depolymerization of HSP polymers than F. chiloensis. With the exception of PME, the activity of all assayed enzymes was higher in F. chiloensis than in F. ×ananassa fruits. The role of PG and EGase seems to be central in both species: these enzymes are correlated to the decrease in the content of HSP and NaOH-soluble polymers (NSP fraction), respectively. In addition, activity levels of βGal and βXyl were significantly correlated with fruit firmness reduction in F. chiloensis and F. ×ananassa, respectively. © 2010 Elsevier B.V. All rights reserved.Fil: Figueroa, Carlos R.. Universidad de Talca; ChileFil: Rosli, Hernan Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Herrera, Raúl. Universidad de Talca; ChileFil: Moya León, María A.. Universidad de Talca; ChileElsevier Science2010-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/152164Figueroa, Carlos R.; Rosli, Hernan Guillermo; Civello, Pedro Marcos; Martinez, Gustavo Adolfo; Herrera, Raúl; et al.; Changes in cell wall polysaccharides and cell wall degrading enzymes during ripening of Fragaria chiloensis and Fragaria ×ananassa fruits; Elsevier Science; Scientia Horticulturae; 124; 4; 01-5-2010; 454-4620304-4238CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2010.02.003info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304423810000476info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:16Zoai:ri.conicet.gov.ar:11336/152164instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:16.493CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Changes in cell wall polysaccharides and cell wall degrading enzymes during ripening of Fragaria chiloensis and Fragaria ×ananassa fruits
title Changes in cell wall polysaccharides and cell wall degrading enzymes during ripening of Fragaria chiloensis and Fragaria ×ananassa fruits
spellingShingle Changes in cell wall polysaccharides and cell wall degrading enzymes during ripening of Fragaria chiloensis and Fragaria ×ananassa fruits
Figueroa, Carlos R.
CELL WALL POLYMERS
CELL WALL-MODIFYING ENZYMES
FRUIT SOFTENING
STRAWBERRY SPECIES
title_short Changes in cell wall polysaccharides and cell wall degrading enzymes during ripening of Fragaria chiloensis and Fragaria ×ananassa fruits
title_full Changes in cell wall polysaccharides and cell wall degrading enzymes during ripening of Fragaria chiloensis and Fragaria ×ananassa fruits
title_fullStr Changes in cell wall polysaccharides and cell wall degrading enzymes during ripening of Fragaria chiloensis and Fragaria ×ananassa fruits
title_full_unstemmed Changes in cell wall polysaccharides and cell wall degrading enzymes during ripening of Fragaria chiloensis and Fragaria ×ananassa fruits
title_sort Changes in cell wall polysaccharides and cell wall degrading enzymes during ripening of Fragaria chiloensis and Fragaria ×ananassa fruits
dc.creator.none.fl_str_mv Figueroa, Carlos R.
Rosli, Hernan Guillermo
Civello, Pedro Marcos
Martinez, Gustavo Adolfo
Herrera, Raúl
Moya León, María A.
author Figueroa, Carlos R.
author_facet Figueroa, Carlos R.
Rosli, Hernan Guillermo
Civello, Pedro Marcos
Martinez, Gustavo Adolfo
Herrera, Raúl
Moya León, María A.
author_role author
author2 Rosli, Hernan Guillermo
Civello, Pedro Marcos
Martinez, Gustavo Adolfo
Herrera, Raúl
Moya León, María A.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CELL WALL POLYMERS
CELL WALL-MODIFYING ENZYMES
FRUIT SOFTENING
STRAWBERRY SPECIES
topic CELL WALL POLYMERS
CELL WALL-MODIFYING ENZYMES
FRUIT SOFTENING
STRAWBERRY SPECIES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Chilean strawberry (Fragaria chiloensis) is arising as a new fruit crop that can diversify the world supply of berries. Fruit softening is thought to be linked to an extensive modification of pectin and hemicellulosic cell wall fractions which in turn is determined to a great extent by the action of some cell wall-modifying enzymes. The objective of this work was to compare cell wall changes and the enzyme activities of pectin methylesterase (PME), polygalacturonase (PG), endoglucanase (EGase), α-arabinofuranosidase (AFase), β-galactosidase (βGal), and β-xylosidase (βXyl) between F. chiloensis and Fragaria ×ananassa (cv. Chandler) at three fruit developmental stages: large green (LG), turning (T) and ripe (R). A rapid decrease in fruit firmness between LG and T stages was observed in both species; nevertheless firmness reduction in F. chiloensis was steeper than in F. ×ananassa. This could be related to a faster loss of HCl-soluble polymers (HSP fraction) presented in F. chiloensis fruit. However, commercial F. ×ananassa showed a greater depolymerization of HSP polymers than F. chiloensis. With the exception of PME, the activity of all assayed enzymes was higher in F. chiloensis than in F. ×ananassa fruits. The role of PG and EGase seems to be central in both species: these enzymes are correlated to the decrease in the content of HSP and NaOH-soluble polymers (NSP fraction), respectively. In addition, activity levels of βGal and βXyl were significantly correlated with fruit firmness reduction in F. chiloensis and F. ×ananassa, respectively. © 2010 Elsevier B.V. All rights reserved.
Fil: Figueroa, Carlos R.. Universidad de Talca; Chile
Fil: Rosli, Hernan Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Civello, Pedro Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Martinez, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Herrera, Raúl. Universidad de Talca; Chile
Fil: Moya León, María A.. Universidad de Talca; Chile
description Chilean strawberry (Fragaria chiloensis) is arising as a new fruit crop that can diversify the world supply of berries. Fruit softening is thought to be linked to an extensive modification of pectin and hemicellulosic cell wall fractions which in turn is determined to a great extent by the action of some cell wall-modifying enzymes. The objective of this work was to compare cell wall changes and the enzyme activities of pectin methylesterase (PME), polygalacturonase (PG), endoglucanase (EGase), α-arabinofuranosidase (AFase), β-galactosidase (βGal), and β-xylosidase (βXyl) between F. chiloensis and Fragaria ×ananassa (cv. Chandler) at three fruit developmental stages: large green (LG), turning (T) and ripe (R). A rapid decrease in fruit firmness between LG and T stages was observed in both species; nevertheless firmness reduction in F. chiloensis was steeper than in F. ×ananassa. This could be related to a faster loss of HCl-soluble polymers (HSP fraction) presented in F. chiloensis fruit. However, commercial F. ×ananassa showed a greater depolymerization of HSP polymers than F. chiloensis. With the exception of PME, the activity of all assayed enzymes was higher in F. chiloensis than in F. ×ananassa fruits. The role of PG and EGase seems to be central in both species: these enzymes are correlated to the decrease in the content of HSP and NaOH-soluble polymers (NSP fraction), respectively. In addition, activity levels of βGal and βXyl were significantly correlated with fruit firmness reduction in F. chiloensis and F. ×ananassa, respectively. © 2010 Elsevier B.V. All rights reserved.
publishDate 2010
dc.date.none.fl_str_mv 2010-05-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/152164
Figueroa, Carlos R.; Rosli, Hernan Guillermo; Civello, Pedro Marcos; Martinez, Gustavo Adolfo; Herrera, Raúl; et al.; Changes in cell wall polysaccharides and cell wall degrading enzymes during ripening of Fragaria chiloensis and Fragaria ×ananassa fruits; Elsevier Science; Scientia Horticulturae; 124; 4; 01-5-2010; 454-462
0304-4238
CONICET Digital
CONICET
url http://hdl.handle.net/11336/152164
identifier_str_mv Figueroa, Carlos R.; Rosli, Hernan Guillermo; Civello, Pedro Marcos; Martinez, Gustavo Adolfo; Herrera, Raúl; et al.; Changes in cell wall polysaccharides and cell wall degrading enzymes during ripening of Fragaria chiloensis and Fragaria ×ananassa fruits; Elsevier Science; Scientia Horticulturae; 124; 4; 01-5-2010; 454-462
0304-4238
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2010.02.003
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304423810000476
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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