The carbohydrate-binding module of <i>Fragaria</i> × <i>ananassa</i> expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro"...
- Autores
- Nardi, Cristina Fernanda; Escudero, Cristian; Villarreal, Natalia Marina; Martínez, Gustavo Adolfo; Civello, Pedro Marcos
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A putative carbohydrate binding module (CBM) from strawberry (Fragaria × ananassa Duch.) expansin 2 (CBM-FaExp2) was cloned and the encoding protein was over-expressed in Escherichia coli and purified in order to evaluate its capacity to bind different cell wall polysaccharides “in vitro”. The protein CBM-FaExp2 bound to microcrystalline cellulose, xylan and pectin with different affinities (Kad = 33.6 ± 0.44 mL g⁻¹, Kad = 11.37 ± 0.87 mL g⁻¹, Kad = 10.4 ± 0.19 mL g⁻¹, respectively). According to “in vitro” enzyme assays, this CBM is able to decrease the activity of cell wall degrading enzymes such as polygalacturonase, endo-glucanase, pectinase and xylanase, probably because the binding of CBM-FaExp2 to the different substrates interferes with enzyme activity. The results suggest that expansins would bind not only cellulose but also a wide range of cell wall polymers.
Facultad de Ciencias Exactas
Instituto de Fisiología Vegetal - Materia
-
Ciencias Exactas
Ciencias Agrarias
CBM
Cell wall
Expansin
Hydrolases
Strawberry - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/131997
Ver los metadatos del registro completo
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The carbohydrate-binding module of <i>Fragaria</i> × <i>ananassa</i> expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro"Nardi, Cristina FernandaEscudero, CristianVillarreal, Natalia MarinaMartínez, Gustavo AdolfoCivello, Pedro MarcosCiencias ExactasCiencias AgrariasCBMCell wallExpansinHydrolasesStrawberryA putative carbohydrate binding module (CBM) from strawberry (<i>Fragaria</i> × ananassa</i> Duch.) expansin 2 (CBM-FaExp2) was cloned and the encoding protein was over-expressed in <i>Escherichia coli</i> and purified in order to evaluate its capacity to bind different cell wall polysaccharides “in vitro”. The protein CBM-FaExp2 bound to microcrystalline cellulose, xylan and pectin with different affinities (K<sub>ad</sub> = 33.6 ± 0.44 mL g⁻¹, K<sub>ad</sub> = 11.37 ± 0.87 mL g⁻¹, K<sub>ad</sub> = 10.4 ± 0.19 mL g⁻¹, respectively). According to “in vitro” enzyme assays, this CBM is able to decrease the activity of cell wall degrading enzymes such as polygalacturonase, endo-glucanase, pectinase and xylanase, probably because the binding of CBM-FaExp2 to the different substrates interferes with enzyme activity. The results suggest that expansins would bind not only cellulose but also a wide range of cell wall polymers.Facultad de Ciencias ExactasInstituto de Fisiología Vegetal2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf151-159http://sedici.unlp.edu.ar/handle/10915/131997enginfo:eu-repo/semantics/altIdentifier/issn/1618-0860info:eu-repo/semantics/altIdentifier/issn/0918-9440info:eu-repo/semantics/altIdentifier/issn/0006-808Xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10265-012-0504-8info:eu-repo/semantics/altIdentifier/pmid/22752710info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-10T12:35:04Zoai:sedici.unlp.edu.ar:10915/131997Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-10 12:35:04.868SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
The carbohydrate-binding module of <i>Fragaria</i> × <i>ananassa</i> expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro" |
| title |
The carbohydrate-binding module of <i>Fragaria</i> × <i>ananassa</i> expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro" |
| spellingShingle |
The carbohydrate-binding module of <i>Fragaria</i> × <i>ananassa</i> expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro" Nardi, Cristina Fernanda Ciencias Exactas Ciencias Agrarias CBM Cell wall Expansin Hydrolases Strawberry |
| title_short |
The carbohydrate-binding module of <i>Fragaria</i> × <i>ananassa</i> expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro" |
| title_full |
The carbohydrate-binding module of <i>Fragaria</i> × <i>ananassa</i> expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro" |
| title_fullStr |
The carbohydrate-binding module of <i>Fragaria</i> × <i>ananassa</i> expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro" |
| title_full_unstemmed |
The carbohydrate-binding module of <i>Fragaria</i> × <i>ananassa</i> expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro" |
| title_sort |
The carbohydrate-binding module of <i>Fragaria</i> × <i>ananassa</i> expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro" |
| dc.creator.none.fl_str_mv |
Nardi, Cristina Fernanda Escudero, Cristian Villarreal, Natalia Marina Martínez, Gustavo Adolfo Civello, Pedro Marcos |
| author |
Nardi, Cristina Fernanda |
| author_facet |
Nardi, Cristina Fernanda Escudero, Cristian Villarreal, Natalia Marina Martínez, Gustavo Adolfo Civello, Pedro Marcos |
| author_role |
author |
| author2 |
Escudero, Cristian Villarreal, Natalia Marina Martínez, Gustavo Adolfo Civello, Pedro Marcos |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
Ciencias Exactas Ciencias Agrarias CBM Cell wall Expansin Hydrolases Strawberry |
| topic |
Ciencias Exactas Ciencias Agrarias CBM Cell wall Expansin Hydrolases Strawberry |
| dc.description.none.fl_txt_mv |
A putative carbohydrate binding module (CBM) from strawberry (<i>Fragaria</i> × ananassa</i> Duch.) expansin 2 (CBM-FaExp2) was cloned and the encoding protein was over-expressed in <i>Escherichia coli</i> and purified in order to evaluate its capacity to bind different cell wall polysaccharides “in vitro”. The protein CBM-FaExp2 bound to microcrystalline cellulose, xylan and pectin with different affinities (K<sub>ad</sub> = 33.6 ± 0.44 mL g⁻¹, K<sub>ad</sub> = 11.37 ± 0.87 mL g⁻¹, K<sub>ad</sub> = 10.4 ± 0.19 mL g⁻¹, respectively). According to “in vitro” enzyme assays, this CBM is able to decrease the activity of cell wall degrading enzymes such as polygalacturonase, endo-glucanase, pectinase and xylanase, probably because the binding of CBM-FaExp2 to the different substrates interferes with enzyme activity. The results suggest that expansins would bind not only cellulose but also a wide range of cell wall polymers. Facultad de Ciencias Exactas Instituto de Fisiología Vegetal |
| description |
A putative carbohydrate binding module (CBM) from strawberry (<i>Fragaria</i> × ananassa</i> Duch.) expansin 2 (CBM-FaExp2) was cloned and the encoding protein was over-expressed in <i>Escherichia coli</i> and purified in order to evaluate its capacity to bind different cell wall polysaccharides “in vitro”. The protein CBM-FaExp2 bound to microcrystalline cellulose, xylan and pectin with different affinities (K<sub>ad</sub> = 33.6 ± 0.44 mL g⁻¹, K<sub>ad</sub> = 11.37 ± 0.87 mL g⁻¹, K<sub>ad</sub> = 10.4 ± 0.19 mL g⁻¹, respectively). According to “in vitro” enzyme assays, this CBM is able to decrease the activity of cell wall degrading enzymes such as polygalacturonase, endo-glucanase, pectinase and xylanase, probably because the binding of CBM-FaExp2 to the different substrates interferes with enzyme activity. The results suggest that expansins would bind not only cellulose but also a wide range of cell wall polymers. |
| publishDate |
2013 |
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2013-01 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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http://sedici.unlp.edu.ar/handle/10915/131997 |
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eng |
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eng |
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