Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice

Autores
Wada, Hiroshi; Chang, Fang Yu; Hatakeyama, Yuto; Erra Balsells, Rosa; Araki, Takuya; Nakano, Hiroshi; Nonami, Hiroshi
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
High night temperature (HNT) often reduces yield in field crops. In rice, HNT during the ripening stage diminishes endosperm cell size, resulting in a considerable reduction in final kernel weight; however, little is known about the underlying mechanisms at cell level. In this study, we performed picolitre pressure-probe-electrospray-ionization mass spectrometry to directly determine metabolites in growing inner endosperm cells of intact seeds produced under HNT conditions, combining with 13C feeding and water status measurements including in situ turgor assay. Microscopic observation in the inner zone suggested that approximately 24.2% of decrease in cell expansion rate occurred under HNT at early ripening stage, leading to a reduction in cell volume. It has been shown that HNT-treated plants were subjected to mild shoot water deficit at night and endosperm cell turgor was sustained by a decline in osmotic potential. Cell metabolomics also suggests that active solute accumulation was caused by a partial inhibition of wall and starch biosynthesis under HNT conditions. Because metabolites were detected in the single cells, it is concluded that a partial arrest of cell expansion observed in the inner endosperms was caused by osmotic adjustment at mild water deficit during HNT conditions.
Fil: Wada, Hiroshi. Ehime University; Japón
Fil: Chang, Fang Yu. Ehime University; Japón
Fil: Hatakeyama, Yuto. Ehime University; Japón
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Araki, Takuya. Ehime University; Japón
Fil: Nakano, Hiroshi. Ehime University; Japón
Fil: Nonami, Hiroshi. Ehime University; Japón
Materia
Heat stress on rice
picoPPESI-MS
ESI-MS
Metabolomics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/173005
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in riceWada, HiroshiChang, Fang YuHatakeyama, YutoErra Balsells, RosaAraki, TakuyaNakano, HiroshiNonami, HiroshiHeat stress on ricepicoPPESI-MSESI-MSMetabolomicshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1High night temperature (HNT) often reduces yield in field crops. In rice, HNT during the ripening stage diminishes endosperm cell size, resulting in a considerable reduction in final kernel weight; however, little is known about the underlying mechanisms at cell level. In this study, we performed picolitre pressure-probe-electrospray-ionization mass spectrometry to directly determine metabolites in growing inner endosperm cells of intact seeds produced under HNT conditions, combining with 13C feeding and water status measurements including in situ turgor assay. Microscopic observation in the inner zone suggested that approximately 24.2% of decrease in cell expansion rate occurred under HNT at early ripening stage, leading to a reduction in cell volume. It has been shown that HNT-treated plants were subjected to mild shoot water deficit at night and endosperm cell turgor was sustained by a decline in osmotic potential. Cell metabolomics also suggests that active solute accumulation was caused by a partial inhibition of wall and starch biosynthesis under HNT conditions. Because metabolites were detected in the single cells, it is concluded that a partial arrest of cell expansion observed in the inner endosperms was caused by osmotic adjustment at mild water deficit during HNT conditions.Fil: Wada, Hiroshi. Ehime University; JapónFil: Chang, Fang Yu. Ehime University; JapónFil: Hatakeyama, Yuto. Ehime University; JapónFil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Araki, Takuya. Ehime University; JapónFil: Nakano, Hiroshi. Ehime University; JapónFil: Nonami, Hiroshi. Ehime University; JapónNature Publishing Group2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/173005Wada, Hiroshi; Chang, Fang Yu; Hatakeyama, Yuto; Erra Balsells, Rosa; Araki, Takuya; et al.; Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice; Nature Publishing Group; Scientific Reports; 11; 1; 2-2021; 1-132045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-021-83870-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:36:06Zoai:ri.conicet.gov.ar:11336/173005instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:36:07.28CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice
title Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice
spellingShingle Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice
Wada, Hiroshi
Heat stress on rice
picoPPESI-MS
ESI-MS
Metabolomics
title_short Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice
title_full Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice
title_fullStr Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice
title_full_unstemmed Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice
title_sort Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice
dc.creator.none.fl_str_mv Wada, Hiroshi
Chang, Fang Yu
Hatakeyama, Yuto
Erra Balsells, Rosa
Araki, Takuya
Nakano, Hiroshi
Nonami, Hiroshi
author Wada, Hiroshi
author_facet Wada, Hiroshi
Chang, Fang Yu
Hatakeyama, Yuto
Erra Balsells, Rosa
Araki, Takuya
Nakano, Hiroshi
Nonami, Hiroshi
author_role author
author2 Chang, Fang Yu
Hatakeyama, Yuto
Erra Balsells, Rosa
Araki, Takuya
Nakano, Hiroshi
Nonami, Hiroshi
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Heat stress on rice
picoPPESI-MS
ESI-MS
Metabolomics
topic Heat stress on rice
picoPPESI-MS
ESI-MS
Metabolomics
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv High night temperature (HNT) often reduces yield in field crops. In rice, HNT during the ripening stage diminishes endosperm cell size, resulting in a considerable reduction in final kernel weight; however, little is known about the underlying mechanisms at cell level. In this study, we performed picolitre pressure-probe-electrospray-ionization mass spectrometry to directly determine metabolites in growing inner endosperm cells of intact seeds produced under HNT conditions, combining with 13C feeding and water status measurements including in situ turgor assay. Microscopic observation in the inner zone suggested that approximately 24.2% of decrease in cell expansion rate occurred under HNT at early ripening stage, leading to a reduction in cell volume. It has been shown that HNT-treated plants were subjected to mild shoot water deficit at night and endosperm cell turgor was sustained by a decline in osmotic potential. Cell metabolomics also suggests that active solute accumulation was caused by a partial inhibition of wall and starch biosynthesis under HNT conditions. Because metabolites were detected in the single cells, it is concluded that a partial arrest of cell expansion observed in the inner endosperms was caused by osmotic adjustment at mild water deficit during HNT conditions.
Fil: Wada, Hiroshi. Ehime University; Japón
Fil: Chang, Fang Yu. Ehime University; Japón
Fil: Hatakeyama, Yuto. Ehime University; Japón
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Araki, Takuya. Ehime University; Japón
Fil: Nakano, Hiroshi. Ehime University; Japón
Fil: Nonami, Hiroshi. Ehime University; Japón
description High night temperature (HNT) often reduces yield in field crops. In rice, HNT during the ripening stage diminishes endosperm cell size, resulting in a considerable reduction in final kernel weight; however, little is known about the underlying mechanisms at cell level. In this study, we performed picolitre pressure-probe-electrospray-ionization mass spectrometry to directly determine metabolites in growing inner endosperm cells of intact seeds produced under HNT conditions, combining with 13C feeding and water status measurements including in situ turgor assay. Microscopic observation in the inner zone suggested that approximately 24.2% of decrease in cell expansion rate occurred under HNT at early ripening stage, leading to a reduction in cell volume. It has been shown that HNT-treated plants were subjected to mild shoot water deficit at night and endosperm cell turgor was sustained by a decline in osmotic potential. Cell metabolomics also suggests that active solute accumulation was caused by a partial inhibition of wall and starch biosynthesis under HNT conditions. Because metabolites were detected in the single cells, it is concluded that a partial arrest of cell expansion observed in the inner endosperms was caused by osmotic adjustment at mild water deficit during HNT conditions.
publishDate 2021
dc.date.none.fl_str_mv 2021-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/173005
Wada, Hiroshi; Chang, Fang Yu; Hatakeyama, Yuto; Erra Balsells, Rosa; Araki, Takuya; et al.; Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice; Nature Publishing Group; Scientific Reports; 11; 1; 2-2021; 1-13
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/173005
identifier_str_mv Wada, Hiroshi; Chang, Fang Yu; Hatakeyama, Yuto; Erra Balsells, Rosa; Araki, Takuya; et al.; Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice; Nature Publishing Group; Scientific Reports; 11; 1; 2-2021; 1-13
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-021-83870-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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