On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in rice

Autores
Wada, Hiroshi; Hatakeyama, Yuto; Nakashima, Taiken; Nonami, Hiroshi; Erra Balsells, Rosa; Hakata, Makoto; Nakata, Keisuke; Hiraoka, Kenzo; Onda, Yayoi; Nakano, Hiroshi
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although a loss of healthy pollen grains induced by metabolic heat responses has been indicated to be a major cause of heat-induced spikelet sterility under global climate change, to date detailed information at pollen level has been lacking due to the technical limitations. In this study, we used picolitre pressure-probe-electrospray-ionization mass spectrometry (picoPPESI-MS) to directly determine the metabolites in heat-treated single mature pollen grains in two cultivars, heat-tolerant cultivar, N22 and heat-sensitive cultivar, Koshihikari. Heat-induced spikelet fertility in N22 and Koshihikari was 90.0% and 46.8%, respectively. While no treatment difference in in vitro pollen viability was observed in each cultivar, contrasting varietal differences in phosphatidylinositol (PI)(34:3) have been detected in mature pollen, together with other 106 metabolites. Greater PI content was detected in N22 pollen regardless of the treatment, but not for Koshihikari pollen. In contrast, there was little detection for phosphoinositide in the single mature pollen grains in both cultivars. Our findings indicate that picoPPESI-MS analysis can efficiently identify the metabolites in intact single pollen. Since PI is a precursor of phosphoinositide that induces multiple signaling for pollen germination and tube growth, the active synthesis of PI(34:3) prior to germination may be closely associated with sustaining spikelet fertility even at high temperatures.
Fil: Wada, Hiroshi. Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization; Japón
Fil: Hatakeyama, Yuto. Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization; Japón
Fil: Nakashima, Taiken. Hokkaido University; Japón
Fil: Nonami, Hiroshi. Ehime University; Japón
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Hakata, Makoto. Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization; Japón
Fil: Nakata, Keisuke. Ehime University; Japón
Fil: Hiraoka, Kenzo. University Of Yamanashi; Japón
Fil: Onda, Yayoi. Ehime University; Japón
Fil: Nakano, Hiroshi. Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization; Japón
Materia
Polen
Rice
PicoPPESI-MS
sterile cause
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/170726
Acceder
id CONICETDig_2fc68165d63483e93b2b98454abd6006
oai_identifier_str oai:ri.conicet.gov.ar:11336/170726
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in riceWada, HiroshiHatakeyama, YutoNakashima, TaikenNonami, HiroshiErra Balsells, RosaHakata, MakotoNakata, KeisukeHiraoka, KenzoOnda, YayoiNakano, HiroshiPolenRicePicoPPESI-MSsterile causehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Although a loss of healthy pollen grains induced by metabolic heat responses has been indicated to be a major cause of heat-induced spikelet sterility under global climate change, to date detailed information at pollen level has been lacking due to the technical limitations. In this study, we used picolitre pressure-probe-electrospray-ionization mass spectrometry (picoPPESI-MS) to directly determine the metabolites in heat-treated single mature pollen grains in two cultivars, heat-tolerant cultivar, N22 and heat-sensitive cultivar, Koshihikari. Heat-induced spikelet fertility in N22 and Koshihikari was 90.0% and 46.8%, respectively. While no treatment difference in in vitro pollen viability was observed in each cultivar, contrasting varietal differences in phosphatidylinositol (PI)(34:3) have been detected in mature pollen, together with other 106 metabolites. Greater PI content was detected in N22 pollen regardless of the treatment, but not for Koshihikari pollen. In contrast, there was little detection for phosphoinositide in the single mature pollen grains in both cultivars. Our findings indicate that picoPPESI-MS analysis can efficiently identify the metabolites in intact single pollen. Since PI is a precursor of phosphoinositide that induces multiple signaling for pollen germination and tube growth, the active synthesis of PI(34:3) prior to germination may be closely associated with sustaining spikelet fertility even at high temperatures.Fil: Wada, Hiroshi. Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization; JapónFil: Hatakeyama, Yuto. Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization; JapónFil: Nakashima, Taiken. Hokkaido University; JapónFil: Nonami, Hiroshi. Ehime University; JapónFil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Hakata, Makoto. Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization; JapónFil: Nakata, Keisuke. Ehime University; JapónFil: Hiraoka, Kenzo. University Of Yamanashi; JapónFil: Onda, Yayoi. Ehime University; JapónFil: Nakano, Hiroshi. Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization; JapónNature2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/170726Wada, Hiroshi; Hatakeyama, Yuto; Nakashima, Taiken; Nonami, Hiroshi; Erra Balsells, Rosa; et al.; On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in rice; Nature; Scientific Reports; 10; 1; 12-2020; 213-2242045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-58869-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:48:56Zoai:ri.conicet.gov.ar:11336/170726instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:48:57.194CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in rice
title On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in rice
spellingShingle On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in rice
Wada, Hiroshi
Polen
Rice
PicoPPESI-MS
sterile cause
title_short On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in rice
title_full On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in rice
title_fullStr On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in rice
title_full_unstemmed On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in rice
title_sort On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in rice
dc.creator.none.fl_str_mv Wada, Hiroshi
Hatakeyama, Yuto
Nakashima, Taiken
Nonami, Hiroshi
Erra Balsells, Rosa
Hakata, Makoto
Nakata, Keisuke
Hiraoka, Kenzo
Onda, Yayoi
Nakano, Hiroshi
author Wada, Hiroshi
author_facet Wada, Hiroshi
Hatakeyama, Yuto
Nakashima, Taiken
Nonami, Hiroshi
Erra Balsells, Rosa
Hakata, Makoto
Nakata, Keisuke
Hiraoka, Kenzo
Onda, Yayoi
Nakano, Hiroshi
author_role author
author2 Hatakeyama, Yuto
Nakashima, Taiken
Nonami, Hiroshi
Erra Balsells, Rosa
Hakata, Makoto
Nakata, Keisuke
Hiraoka, Kenzo
Onda, Yayoi
Nakano, Hiroshi
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Polen
Rice
PicoPPESI-MS
sterile cause
topic Polen
Rice
PicoPPESI-MS
sterile cause
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Although a loss of healthy pollen grains induced by metabolic heat responses has been indicated to be a major cause of heat-induced spikelet sterility under global climate change, to date detailed information at pollen level has been lacking due to the technical limitations. In this study, we used picolitre pressure-probe-electrospray-ionization mass spectrometry (picoPPESI-MS) to directly determine the metabolites in heat-treated single mature pollen grains in two cultivars, heat-tolerant cultivar, N22 and heat-sensitive cultivar, Koshihikari. Heat-induced spikelet fertility in N22 and Koshihikari was 90.0% and 46.8%, respectively. While no treatment difference in in vitro pollen viability was observed in each cultivar, contrasting varietal differences in phosphatidylinositol (PI)(34:3) have been detected in mature pollen, together with other 106 metabolites. Greater PI content was detected in N22 pollen regardless of the treatment, but not for Koshihikari pollen. In contrast, there was little detection for phosphoinositide in the single mature pollen grains in both cultivars. Our findings indicate that picoPPESI-MS analysis can efficiently identify the metabolites in intact single pollen. Since PI is a precursor of phosphoinositide that induces multiple signaling for pollen germination and tube growth, the active synthesis of PI(34:3) prior to germination may be closely associated with sustaining spikelet fertility even at high temperatures.
Fil: Wada, Hiroshi. Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization; Japón
Fil: Hatakeyama, Yuto. Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization; Japón
Fil: Nakashima, Taiken. Hokkaido University; Japón
Fil: Nonami, Hiroshi. Ehime University; Japón
Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Hakata, Makoto. Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization; Japón
Fil: Nakata, Keisuke. Ehime University; Japón
Fil: Hiraoka, Kenzo. University Of Yamanashi; Japón
Fil: Onda, Yayoi. Ehime University; Japón
Fil: Nakano, Hiroshi. Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization; Japón
description Although a loss of healthy pollen grains induced by metabolic heat responses has been indicated to be a major cause of heat-induced spikelet sterility under global climate change, to date detailed information at pollen level has been lacking due to the technical limitations. In this study, we used picolitre pressure-probe-electrospray-ionization mass spectrometry (picoPPESI-MS) to directly determine the metabolites in heat-treated single mature pollen grains in two cultivars, heat-tolerant cultivar, N22 and heat-sensitive cultivar, Koshihikari. Heat-induced spikelet fertility in N22 and Koshihikari was 90.0% and 46.8%, respectively. While no treatment difference in in vitro pollen viability was observed in each cultivar, contrasting varietal differences in phosphatidylinositol (PI)(34:3) have been detected in mature pollen, together with other 106 metabolites. Greater PI content was detected in N22 pollen regardless of the treatment, but not for Koshihikari pollen. In contrast, there was little detection for phosphoinositide in the single mature pollen grains in both cultivars. Our findings indicate that picoPPESI-MS analysis can efficiently identify the metabolites in intact single pollen. Since PI is a precursor of phosphoinositide that induces multiple signaling for pollen germination and tube growth, the active synthesis of PI(34:3) prior to germination may be closely associated with sustaining spikelet fertility even at high temperatures.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/170726
Wada, Hiroshi; Hatakeyama, Yuto; Nakashima, Taiken; Nonami, Hiroshi; Erra Balsells, Rosa; et al.; On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in rice; Nature; Scientific Reports; 10; 1; 12-2020; 213-224
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/170726
identifier_str_mv Wada, Hiroshi; Hatakeyama, Yuto; Nakashima, Taiken; Nonami, Hiroshi; Erra Balsells, Rosa; et al.; On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in rice; Nature; Scientific Reports; 10; 1; 12-2020; 213-224
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-58869-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature
publisher.none.fl_str_mv Nature
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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