An oomycete effector subverts host vesicle trafficking to channel starvation-induced autophagy to the pathogen interface

Autores
Pandey, Pooja; Leary, Alexandre Y.; Tumtas, Yasin; Savage, Zachary; Dagvadorj, Bayantes; Duggan, Cian; Yuen, Enoch L.H.; Sanguankiattichai, Nattapong; Tan, Emily; Khandare, Virendrasinh; Connerton, Amber J.; Yunusov, Temur; Madalinski, Mathias; Mirkin, Federico Gabriel; Schornack, Sebastian; Dagdas, Yasin; Kamoun, Sophien; Bozkurt, Osman Tolga
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Eukaryotic cells deploy autophagy to eliminate invading microbes. In turn, pathogens have evolved effector proteins to counteract antimicrobial autophagy. How adapted pathogens co-opt autophagy for their own benefit is poorly understood. The Irish famine pathogen Phytophthora infestans secretes the effector protein PexRD54 that selectively activates an unknown plant autophagy pathway that antagonizes antimicrobial autophagy at the pathogen interface. Here, we show that PexRD54 induces autophagosome formation by bridging vesicles decorated by the small GTPase Rab8a with autophagic compartments labeled by the core autophagy protein ATG8CL. Rab8a is required for pathogen-triggered and starvation-induced but not antimicrobial autophagy, revealing specific trafficking pathways underpin selective autophagy. By subverting Rab8a-mediated vesicle trafficking, PexRD54 utilizes lipid droplets to facilitate biogenesis of autophagosomes diverted to pathogen feeding sites. Altogether, we show that PexRD54 mimics starvation-induced autophagy to subvert endomembrane trafficking at the host-pathogen interface, revealing how effectors bridge distinct host compartments to expedite colonization.
Fil: Pandey, Pooja. Imperial College London; Reino Unido
Fil: Leary, Alexandre Y.. Imperial College London; Reino Unido
Fil: Tumtas, Yasin. Imperial College London; Reino Unido
Fil: Savage, Zachary. Imperial College London; Reino Unido
Fil: Dagvadorj, Bayantes. Imperial College London; Reino Unido
Fil: Duggan, Cian. Imperial College London; Reino Unido
Fil: Yuen, Enoch L.H.. Imperial College London; Reino Unido
Fil: Sanguankiattichai, Nattapong. Imperial College London; Reino Unido
Fil: Tan, Emily. Imperial College London; Reino Unido
Fil: Khandare, Virendrasinh. Imperial College London; Reino Unido
Fil: Connerton, Amber J.. Imperial College London; Reino Unido
Fil: Yunusov, Temur. University of Cambridge; Reino Unido
Fil: Madalinski, Mathias. Gregor Mendel Institute; Austria
Fil: Mirkin, Federico Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Schornack, Sebastian. University of Cambridge; Reino Unido
Fil: Dagdas, Yasin. Gregor Mendel Institute; Austria
Fil: Kamoun, Sophien. University of East Anglia; Reino Unido
Fil: Bozkurt, Osman Tolga. Imperial College London; Reino Unido
Materia
PHYTOPHTHORA INFESTANS
AUTOPHAGY
EFFECTOR
RAB8
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/158819
Acceder
id CONICETDig_86123acd9d052b8b7f6b40059f369c56
oai_identifier_str oai:ri.conicet.gov.ar:11336/158819
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An oomycete effector subverts host vesicle trafficking to channel starvation-induced autophagy to the pathogen interfacePandey, PoojaLeary, Alexandre Y.Tumtas, YasinSavage, ZacharyDagvadorj, BayantesDuggan, CianYuen, Enoch L.H.Sanguankiattichai, NattapongTan, EmilyKhandare, VirendrasinhConnerton, Amber J.Yunusov, TemurMadalinski, MathiasMirkin, Federico GabrielSchornack, SebastianDagdas, YasinKamoun, SophienBozkurt, Osman TolgaPHYTOPHTHORA INFESTANSAUTOPHAGYEFFECTORRAB8https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Eukaryotic cells deploy autophagy to eliminate invading microbes. In turn, pathogens have evolved effector proteins to counteract antimicrobial autophagy. How adapted pathogens co-opt autophagy for their own benefit is poorly understood. The Irish famine pathogen Phytophthora infestans secretes the effector protein PexRD54 that selectively activates an unknown plant autophagy pathway that antagonizes antimicrobial autophagy at the pathogen interface. Here, we show that PexRD54 induces autophagosome formation by bridging vesicles decorated by the small GTPase Rab8a with autophagic compartments labeled by the core autophagy protein ATG8CL. Rab8a is required for pathogen-triggered and starvation-induced but not antimicrobial autophagy, revealing specific trafficking pathways underpin selective autophagy. By subverting Rab8a-mediated vesicle trafficking, PexRD54 utilizes lipid droplets to facilitate biogenesis of autophagosomes diverted to pathogen feeding sites. Altogether, we show that PexRD54 mimics starvation-induced autophagy to subvert endomembrane trafficking at the host-pathogen interface, revealing how effectors bridge distinct host compartments to expedite colonization.Fil: Pandey, Pooja. Imperial College London; Reino UnidoFil: Leary, Alexandre Y.. Imperial College London; Reino UnidoFil: Tumtas, Yasin. Imperial College London; Reino UnidoFil: Savage, Zachary. Imperial College London; Reino UnidoFil: Dagvadorj, Bayantes. Imperial College London; Reino UnidoFil: Duggan, Cian. Imperial College London; Reino UnidoFil: Yuen, Enoch L.H.. Imperial College London; Reino UnidoFil: Sanguankiattichai, Nattapong. Imperial College London; Reino UnidoFil: Tan, Emily. Imperial College London; Reino UnidoFil: Khandare, Virendrasinh. Imperial College London; Reino UnidoFil: Connerton, Amber J.. Imperial College London; Reino UnidoFil: Yunusov, Temur. University of Cambridge; Reino UnidoFil: Madalinski, Mathias. Gregor Mendel Institute; AustriaFil: Mirkin, Federico Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Schornack, Sebastian. University of Cambridge; Reino UnidoFil: Dagdas, Yasin. Gregor Mendel Institute; AustriaFil: Kamoun, Sophien. University of East Anglia; Reino UnidoFil: Bozkurt, Osman Tolga. Imperial College London; Reino UnidoeLife Sciences Publications Ltd2021-08-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/158819Pandey, Pooja; Leary, Alexandre Y.; Tumtas, Yasin; Savage, Zachary; Dagvadorj, Bayantes; et al.; An oomycete effector subverts host vesicle trafficking to channel starvation-induced autophagy to the pathogen interface; eLife Sciences Publications Ltd; eLife; 10; 23-8-2021; 1-352050-084XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/65285info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.65285info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:33Zoai:ri.conicet.gov.ar:11336/158819instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:33.834CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An oomycete effector subverts host vesicle trafficking to channel starvation-induced autophagy to the pathogen interface
title An oomycete effector subverts host vesicle trafficking to channel starvation-induced autophagy to the pathogen interface
spellingShingle An oomycete effector subverts host vesicle trafficking to channel starvation-induced autophagy to the pathogen interface
Pandey, Pooja
PHYTOPHTHORA INFESTANS
AUTOPHAGY
EFFECTOR
RAB8
title_short An oomycete effector subverts host vesicle trafficking to channel starvation-induced autophagy to the pathogen interface
title_full An oomycete effector subverts host vesicle trafficking to channel starvation-induced autophagy to the pathogen interface
title_fullStr An oomycete effector subverts host vesicle trafficking to channel starvation-induced autophagy to the pathogen interface
title_full_unstemmed An oomycete effector subverts host vesicle trafficking to channel starvation-induced autophagy to the pathogen interface
title_sort An oomycete effector subverts host vesicle trafficking to channel starvation-induced autophagy to the pathogen interface
dc.creator.none.fl_str_mv Pandey, Pooja
Leary, Alexandre Y.
Tumtas, Yasin
Savage, Zachary
Dagvadorj, Bayantes
Duggan, Cian
Yuen, Enoch L.H.
Sanguankiattichai, Nattapong
Tan, Emily
Khandare, Virendrasinh
Connerton, Amber J.
Yunusov, Temur
Madalinski, Mathias
Mirkin, Federico Gabriel
Schornack, Sebastian
Dagdas, Yasin
Kamoun, Sophien
Bozkurt, Osman Tolga
author Pandey, Pooja
author_facet Pandey, Pooja
Leary, Alexandre Y.
Tumtas, Yasin
Savage, Zachary
Dagvadorj, Bayantes
Duggan, Cian
Yuen, Enoch L.H.
Sanguankiattichai, Nattapong
Tan, Emily
Khandare, Virendrasinh
Connerton, Amber J.
Yunusov, Temur
Madalinski, Mathias
Mirkin, Federico Gabriel
Schornack, Sebastian
Dagdas, Yasin
Kamoun, Sophien
Bozkurt, Osman Tolga
author_role author
author2 Leary, Alexandre Y.
Tumtas, Yasin
Savage, Zachary
Dagvadorj, Bayantes
Duggan, Cian
Yuen, Enoch L.H.
Sanguankiattichai, Nattapong
Tan, Emily
Khandare, Virendrasinh
Connerton, Amber J.
Yunusov, Temur
Madalinski, Mathias
Mirkin, Federico Gabriel
Schornack, Sebastian
Dagdas, Yasin
Kamoun, Sophien
Bozkurt, Osman Tolga
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PHYTOPHTHORA INFESTANS
AUTOPHAGY
EFFECTOR
RAB8
topic PHYTOPHTHORA INFESTANS
AUTOPHAGY
EFFECTOR
RAB8
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Eukaryotic cells deploy autophagy to eliminate invading microbes. In turn, pathogens have evolved effector proteins to counteract antimicrobial autophagy. How adapted pathogens co-opt autophagy for their own benefit is poorly understood. The Irish famine pathogen Phytophthora infestans secretes the effector protein PexRD54 that selectively activates an unknown plant autophagy pathway that antagonizes antimicrobial autophagy at the pathogen interface. Here, we show that PexRD54 induces autophagosome formation by bridging vesicles decorated by the small GTPase Rab8a with autophagic compartments labeled by the core autophagy protein ATG8CL. Rab8a is required for pathogen-triggered and starvation-induced but not antimicrobial autophagy, revealing specific trafficking pathways underpin selective autophagy. By subverting Rab8a-mediated vesicle trafficking, PexRD54 utilizes lipid droplets to facilitate biogenesis of autophagosomes diverted to pathogen feeding sites. Altogether, we show that PexRD54 mimics starvation-induced autophagy to subvert endomembrane trafficking at the host-pathogen interface, revealing how effectors bridge distinct host compartments to expedite colonization.
Fil: Pandey, Pooja. Imperial College London; Reino Unido
Fil: Leary, Alexandre Y.. Imperial College London; Reino Unido
Fil: Tumtas, Yasin. Imperial College London; Reino Unido
Fil: Savage, Zachary. Imperial College London; Reino Unido
Fil: Dagvadorj, Bayantes. Imperial College London; Reino Unido
Fil: Duggan, Cian. Imperial College London; Reino Unido
Fil: Yuen, Enoch L.H.. Imperial College London; Reino Unido
Fil: Sanguankiattichai, Nattapong. Imperial College London; Reino Unido
Fil: Tan, Emily. Imperial College London; Reino Unido
Fil: Khandare, Virendrasinh. Imperial College London; Reino Unido
Fil: Connerton, Amber J.. Imperial College London; Reino Unido
Fil: Yunusov, Temur. University of Cambridge; Reino Unido
Fil: Madalinski, Mathias. Gregor Mendel Institute; Austria
Fil: Mirkin, Federico Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Schornack, Sebastian. University of Cambridge; Reino Unido
Fil: Dagdas, Yasin. Gregor Mendel Institute; Austria
Fil: Kamoun, Sophien. University of East Anglia; Reino Unido
Fil: Bozkurt, Osman Tolga. Imperial College London; Reino Unido
description Eukaryotic cells deploy autophagy to eliminate invading microbes. In turn, pathogens have evolved effector proteins to counteract antimicrobial autophagy. How adapted pathogens co-opt autophagy for their own benefit is poorly understood. The Irish famine pathogen Phytophthora infestans secretes the effector protein PexRD54 that selectively activates an unknown plant autophagy pathway that antagonizes antimicrobial autophagy at the pathogen interface. Here, we show that PexRD54 induces autophagosome formation by bridging vesicles decorated by the small GTPase Rab8a with autophagic compartments labeled by the core autophagy protein ATG8CL. Rab8a is required for pathogen-triggered and starvation-induced but not antimicrobial autophagy, revealing specific trafficking pathways underpin selective autophagy. By subverting Rab8a-mediated vesicle trafficking, PexRD54 utilizes lipid droplets to facilitate biogenesis of autophagosomes diverted to pathogen feeding sites. Altogether, we show that PexRD54 mimics starvation-induced autophagy to subvert endomembrane trafficking at the host-pathogen interface, revealing how effectors bridge distinct host compartments to expedite colonization.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-23
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/158819
Pandey, Pooja; Leary, Alexandre Y.; Tumtas, Yasin; Savage, Zachary; Dagvadorj, Bayantes; et al.; An oomycete effector subverts host vesicle trafficking to channel starvation-induced autophagy to the pathogen interface; eLife Sciences Publications Ltd; eLife; 10; 23-8-2021; 1-35
2050-084X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/158819
identifier_str_mv Pandey, Pooja; Leary, Alexandre Y.; Tumtas, Yasin; Savage, Zachary; Dagvadorj, Bayantes; et al.; An oomycete effector subverts host vesicle trafficking to channel starvation-induced autophagy to the pathogen interface; eLife Sciences Publications Ltd; eLife; 10; 23-8-2021; 1-35
2050-084X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/65285
info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.65285
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv eLife Sciences Publications Ltd
publisher.none.fl_str_mv eLife Sciences Publications Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270049700675584
score 13.13397

Publicaciones similares