Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum

Autores
Giannakopoulou, Artemis; Steele, John F. C.; Segretin, Maria Eugenia; Bozkurt, Tolga; Zhou, Ji; Robatzek, Silke; Banfield, Mark J; Pais, Marina; Kamoun, Sophien
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plants and animals rely on immune receptors, known as nucleotide-binding domain and leucine-rich repeat containing proteins (NB-LRR or NLR), to defend against invading pathogens and activate immune responses. How NLR receptors respond to pathogens is inadequately understood. We previously reported single-residue mutations that expand the response of the potato immune receptor R3a to AVR3aEM, a stealthy effector from the late blight oomycete pathogen Phytophthora infestans. I2, another NLR that mediates resistance to the wilt causing fungus Fusarium oxysporum f. sp. lycopersici, is the tomato ortholog of R3a. We transferred previously identified R3a mutations to I2 to assess the degree to which the resulting I2 mutants have an altered response. We discovered that wild-type I2 protein responds weakly to AVR3a. One mutant in the N-terminal coiled-coil domain, I2I141N, appeared sensitized and displayed markedly increased response to AVR3a. Remarkably, I2I141N conferred partial resistance to P. infestans. Further, I2I141N has an expanded response spectrum to F. oxysporum f. sp. lycopersici effectors compared to the wild-type I2 protein. Our results suggest that synthetic immune receptors can be engineered to confer resistance to phylogenetically divergent pathogens and indicate that knowledge gathered for one NLR could be exploited to improve NLRs from other plant species.
Fil: Giannakopoulou, Artemis. The Sainsbury Laboratory; Reino Unido
Fil: Steele, John F. C.. John Innes Institute; Reino Unido
Fil: Segretin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina
Fil: Bozkurt, Tolga. The Sainsbury Laboratory; Reino Unido. Imperial College London; Reino Unido
Fil: Zhou, Ji. The Sainsbury Laboratory; Reino Unido. The Genome Analysis Centre; Reino Unido
Fil: Robatzek, Silke. The Sainsbury Laboratory; Reino Unido
Fil: Banfield, Mark J. John Innes Institute; Reino Unido
Fil: Pais, Marina. The Sainsbury Laboratory; Reino Unido
Fil: Kamoun, Sophien. The Sainsbury Laboratory; Reino Unido
Materia
Nb-Lrr
R3a
Phytophthora Infestans
I2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4001
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4001
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporumGiannakopoulou, ArtemisSteele, John F. C.Segretin, Maria EugeniaBozkurt, TolgaZhou, JiRobatzek, SilkeBanfield, Mark JPais, MarinaKamoun, SophienNb-LrrR3aPhytophthora InfestansI2https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Plants and animals rely on immune receptors, known as nucleotide-binding domain and leucine-rich repeat containing proteins (NB-LRR or NLR), to defend against invading pathogens and activate immune responses. How NLR receptors respond to pathogens is inadequately understood. We previously reported single-residue mutations that expand the response of the potato immune receptor R3a to AVR3aEM, a stealthy effector from the late blight oomycete pathogen Phytophthora infestans. I2, another NLR that mediates resistance to the wilt causing fungus Fusarium oxysporum f. sp. lycopersici, is the tomato ortholog of R3a. We transferred previously identified R3a mutations to I2 to assess the degree to which the resulting I2 mutants have an altered response. We discovered that wild-type I2 protein responds weakly to AVR3a. One mutant in the N-terminal coiled-coil domain, I2I141N, appeared sensitized and displayed markedly increased response to AVR3a. Remarkably, I2I141N conferred partial resistance to P. infestans. Further, I2I141N has an expanded response spectrum to F. oxysporum f. sp. lycopersici effectors compared to the wild-type I2 protein. Our results suggest that synthetic immune receptors can be engineered to confer resistance to phylogenetically divergent pathogens and indicate that knowledge gathered for one NLR could be exploited to improve NLRs from other plant species.Fil: Giannakopoulou, Artemis. The Sainsbury Laboratory; Reino UnidoFil: Steele, John F. C.. John Innes Institute; Reino UnidoFil: Segretin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; ArgentinaFil: Bozkurt, Tolga. The Sainsbury Laboratory; Reino Unido. Imperial College London; Reino UnidoFil: Zhou, Ji. The Sainsbury Laboratory; Reino Unido. The Genome Analysis Centre; Reino UnidoFil: Robatzek, Silke. The Sainsbury Laboratory; Reino UnidoFil: Banfield, Mark J. John Innes Institute; Reino UnidoFil: Pais, Marina. The Sainsbury Laboratory; Reino UnidoFil: Kamoun, Sophien. The Sainsbury Laboratory; Reino UnidoAmerican Phytopathological Society2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4001Giannakopoulou, Artemis; Steele, John F. C.; Segretin, Maria Eugenia; Bozkurt, Tolga; Zhou, Ji; et al.; Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum; American Phytopathological Society; Molecular Plant-Microbe Interactions; 28; 12; 9-2015; 1316-13290894-0282enginfo:eu-repo/semantics/altIdentifier/url/http://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-07-15-0147-Rinfo:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-07-15-0147-Rinfo:eu-repo/semantics/altIdentifier/issn/0894-0282info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:42Zoai:ri.conicet.gov.ar:11336/4001instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:42.622CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum
title Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum
spellingShingle Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum
Giannakopoulou, Artemis
Nb-Lrr
R3a
Phytophthora Infestans
I2
title_short Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum
title_full Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum
title_fullStr Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum
title_full_unstemmed Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum
title_sort Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum
dc.creator.none.fl_str_mv Giannakopoulou, Artemis
Steele, John F. C.
Segretin, Maria Eugenia
Bozkurt, Tolga
Zhou, Ji
Robatzek, Silke
Banfield, Mark J
Pais, Marina
Kamoun, Sophien
author Giannakopoulou, Artemis
author_facet Giannakopoulou, Artemis
Steele, John F. C.
Segretin, Maria Eugenia
Bozkurt, Tolga
Zhou, Ji
Robatzek, Silke
Banfield, Mark J
Pais, Marina
Kamoun, Sophien
author_role author
author2 Steele, John F. C.
Segretin, Maria Eugenia
Bozkurt, Tolga
Zhou, Ji
Robatzek, Silke
Banfield, Mark J
Pais, Marina
Kamoun, Sophien
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Nb-Lrr
R3a
Phytophthora Infestans
I2
topic Nb-Lrr
R3a
Phytophthora Infestans
I2
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Plants and animals rely on immune receptors, known as nucleotide-binding domain and leucine-rich repeat containing proteins (NB-LRR or NLR), to defend against invading pathogens and activate immune responses. How NLR receptors respond to pathogens is inadequately understood. We previously reported single-residue mutations that expand the response of the potato immune receptor R3a to AVR3aEM, a stealthy effector from the late blight oomycete pathogen Phytophthora infestans. I2, another NLR that mediates resistance to the wilt causing fungus Fusarium oxysporum f. sp. lycopersici, is the tomato ortholog of R3a. We transferred previously identified R3a mutations to I2 to assess the degree to which the resulting I2 mutants have an altered response. We discovered that wild-type I2 protein responds weakly to AVR3a. One mutant in the N-terminal coiled-coil domain, I2I141N, appeared sensitized and displayed markedly increased response to AVR3a. Remarkably, I2I141N conferred partial resistance to P. infestans. Further, I2I141N has an expanded response spectrum to F. oxysporum f. sp. lycopersici effectors compared to the wild-type I2 protein. Our results suggest that synthetic immune receptors can be engineered to confer resistance to phylogenetically divergent pathogens and indicate that knowledge gathered for one NLR could be exploited to improve NLRs from other plant species.
Fil: Giannakopoulou, Artemis. The Sainsbury Laboratory; Reino Unido
Fil: Steele, John F. C.. John Innes Institute; Reino Unido
Fil: Segretin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina
Fil: Bozkurt, Tolga. The Sainsbury Laboratory; Reino Unido. Imperial College London; Reino Unido
Fil: Zhou, Ji. The Sainsbury Laboratory; Reino Unido. The Genome Analysis Centre; Reino Unido
Fil: Robatzek, Silke. The Sainsbury Laboratory; Reino Unido
Fil: Banfield, Mark J. John Innes Institute; Reino Unido
Fil: Pais, Marina. The Sainsbury Laboratory; Reino Unido
Fil: Kamoun, Sophien. The Sainsbury Laboratory; Reino Unido
description Plants and animals rely on immune receptors, known as nucleotide-binding domain and leucine-rich repeat containing proteins (NB-LRR or NLR), to defend against invading pathogens and activate immune responses. How NLR receptors respond to pathogens is inadequately understood. We previously reported single-residue mutations that expand the response of the potato immune receptor R3a to AVR3aEM, a stealthy effector from the late blight oomycete pathogen Phytophthora infestans. I2, another NLR that mediates resistance to the wilt causing fungus Fusarium oxysporum f. sp. lycopersici, is the tomato ortholog of R3a. We transferred previously identified R3a mutations to I2 to assess the degree to which the resulting I2 mutants have an altered response. We discovered that wild-type I2 protein responds weakly to AVR3a. One mutant in the N-terminal coiled-coil domain, I2I141N, appeared sensitized and displayed markedly increased response to AVR3a. Remarkably, I2I141N conferred partial resistance to P. infestans. Further, I2I141N has an expanded response spectrum to F. oxysporum f. sp. lycopersici effectors compared to the wild-type I2 protein. Our results suggest that synthetic immune receptors can be engineered to confer resistance to phylogenetically divergent pathogens and indicate that knowledge gathered for one NLR could be exploited to improve NLRs from other plant species.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4001
Giannakopoulou, Artemis; Steele, John F. C.; Segretin, Maria Eugenia; Bozkurt, Tolga; Zhou, Ji; et al.; Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum; American Phytopathological Society; Molecular Plant-Microbe Interactions; 28; 12; 9-2015; 1316-1329
0894-0282
url http://hdl.handle.net/11336/4001
identifier_str_mv Giannakopoulou, Artemis; Steele, John F. C.; Segretin, Maria Eugenia; Bozkurt, Tolga; Zhou, Ji; et al.; Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum; American Phytopathological Society; Molecular Plant-Microbe Interactions; 28; 12; 9-2015; 1316-1329
0894-0282
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-07-15-0147-R
info:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-07-15-0147-R
info:eu-repo/semantics/altIdentifier/issn/0894-0282
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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