Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the irish potato famine pathogen

Autores
Dagdas, Yasin F.; Pandey, Pooja; Tumtas, Yasin; Sanguankiattichai, Nattapong; Belhaj, Khaoula; Duggan, Cian; Leary, Alexandre Y.; Segretin, Maria Eugenia; Contreras, Mauricio P.; Savage, Zachary; Khandare, Virendrasinh S.; Kamoun, Sophien; Bozkurt, Osman Tolga
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
During plant cell invasion, the oomycete Phytophthora infestans remains enveloped by host-derived membranes whose functional properties are poorly understood. P. infestans secretes a myriad of effector proteins through these interfaces for plant colonization. Recently we showed that the effector protein PexRD54 reprograms host-selective autophagy by antagonising antimicrobial-autophagy receptor Joka2/NBR1 for ATG8CL binding (Dagdas et al., 2016). Here, we show that during infection, ATG8CL/Joka2 labelled defense-related autophagosomes are diverted toward the perimicrobial host membrane to restrict pathogen growth. PexRD54 also localizes to autophagosomes across the perimicrobial membrane, consistent with the view that the pathogen remodels host-microbe interface by co-opting the host autophagy machinery. Furthermore, we show that the host-pathogen interface is a hotspot for autophagosome biogenesis. Notably, overexpression of the early autophagosome biogenesis protein ATG9 enhances plant immunity. Our results implicate selective autophagy in polarized immune responses of plants and point to more complex functions for autophagy than the widely known degradative roles.
Fil: Dagdas, Yasin F.. Norwich Research Park; Reino Unido. Vienna Biocenter; Austria
Fil: Pandey, Pooja. Imperial College London; Reino Unido
Fil: Tumtas, Yasin. Imperial College London; Reino Unido
Fil: Sanguankiattichai, Nattapong. Imperial College London; Reino Unido
Fil: Belhaj, Khaoula. Norwich Research Park; Reino Unido
Fil: Duggan, Cian. Imperial College London; Reino Unido
Fil: Leary, Alexandre Y.. Imperial College London; Reino Unido
Fil: Segretin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Contreras, Mauricio P.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Imperial College London; Reino Unido
Fil: Savage, Zachary. Imperial College London; Reino Unido
Fil: Khandare, Virendrasinh S.. Imperial College London; Reino Unido
Fil: Kamoun, Sophien. Norwich Research Park; Reino Unido
Fil: Bozkurt, Osman Tolga. Imperial College London; Reino Unido
Materia
AUTOPHAGY
IMMUNITY
PHYTOPHTHORA INFESTANS
EFFECTOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79890
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79890
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the irish potato famine pathogenDagdas, Yasin F.Pandey, PoojaTumtas, YasinSanguankiattichai, NattapongBelhaj, KhaoulaDuggan, CianLeary, Alexandre Y.Segretin, Maria EugeniaContreras, Mauricio P.Savage, ZacharyKhandare, Virendrasinh S.Kamoun, SophienBozkurt, Osman TolgaAUTOPHAGYIMMUNITYPHYTOPHTHORA INFESTANSEFFECTORhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1During plant cell invasion, the oomycete Phytophthora infestans remains enveloped by host-derived membranes whose functional properties are poorly understood. P. infestans secretes a myriad of effector proteins through these interfaces for plant colonization. Recently we showed that the effector protein PexRD54 reprograms host-selective autophagy by antagonising antimicrobial-autophagy receptor Joka2/NBR1 for ATG8CL binding (Dagdas et al., 2016). Here, we show that during infection, ATG8CL/Joka2 labelled defense-related autophagosomes are diverted toward the perimicrobial host membrane to restrict pathogen growth. PexRD54 also localizes to autophagosomes across the perimicrobial membrane, consistent with the view that the pathogen remodels host-microbe interface by co-opting the host autophagy machinery. Furthermore, we show that the host-pathogen interface is a hotspot for autophagosome biogenesis. Notably, overexpression of the early autophagosome biogenesis protein ATG9 enhances plant immunity. Our results implicate selective autophagy in polarized immune responses of plants and point to more complex functions for autophagy than the widely known degradative roles.Fil: Dagdas, Yasin F.. Norwich Research Park; Reino Unido. Vienna Biocenter; AustriaFil: Pandey, Pooja. Imperial College London; Reino UnidoFil: Tumtas, Yasin. Imperial College London; Reino UnidoFil: Sanguankiattichai, Nattapong. Imperial College London; Reino UnidoFil: Belhaj, Khaoula. Norwich Research Park; Reino UnidoFil: Duggan, Cian. Imperial College London; Reino UnidoFil: Leary, Alexandre Y.. Imperial College London; Reino UnidoFil: Segretin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Contreras, Mauricio P.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Imperial College London; Reino UnidoFil: Savage, Zachary. Imperial College London; Reino UnidoFil: Khandare, Virendrasinh S.. Imperial College London; Reino UnidoFil: Kamoun, Sophien. Norwich Research Park; Reino UnidoFil: Bozkurt, Osman Tolga. Imperial College London; Reino UnidoeLife Sciences Publications Ltd2018-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79890Dagdas, Yasin F.; Pandey, Pooja; Tumtas, Yasin; Sanguankiattichai, Nattapong; Belhaj, Khaoula; et al.; Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the irish potato famine pathogen; eLife Sciences Publications Ltd; eLife; 7; 6-2018; 1-152050-084XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.37476info:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/37476info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:38Zoai:ri.conicet.gov.ar:11336/79890instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:38.378CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the irish potato famine pathogen
title Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the irish potato famine pathogen
spellingShingle Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the irish potato famine pathogen
Dagdas, Yasin F.
AUTOPHAGY
IMMUNITY
PHYTOPHTHORA INFESTANS
EFFECTOR
title_short Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the irish potato famine pathogen
title_full Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the irish potato famine pathogen
title_fullStr Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the irish potato famine pathogen
title_full_unstemmed Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the irish potato famine pathogen
title_sort Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the irish potato famine pathogen
dc.creator.none.fl_str_mv Dagdas, Yasin F.
Pandey, Pooja
Tumtas, Yasin
Sanguankiattichai, Nattapong
Belhaj, Khaoula
Duggan, Cian
Leary, Alexandre Y.
Segretin, Maria Eugenia
Contreras, Mauricio P.
Savage, Zachary
Khandare, Virendrasinh S.
Kamoun, Sophien
Bozkurt, Osman Tolga
author Dagdas, Yasin F.
author_facet Dagdas, Yasin F.
Pandey, Pooja
Tumtas, Yasin
Sanguankiattichai, Nattapong
Belhaj, Khaoula
Duggan, Cian
Leary, Alexandre Y.
Segretin, Maria Eugenia
Contreras, Mauricio P.
Savage, Zachary
Khandare, Virendrasinh S.
Kamoun, Sophien
Bozkurt, Osman Tolga
author_role author
author2 Pandey, Pooja
Tumtas, Yasin
Sanguankiattichai, Nattapong
Belhaj, Khaoula
Duggan, Cian
Leary, Alexandre Y.
Segretin, Maria Eugenia
Contreras, Mauricio P.
Savage, Zachary
Khandare, Virendrasinh S.
Kamoun, Sophien
Bozkurt, Osman Tolga
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AUTOPHAGY
IMMUNITY
PHYTOPHTHORA INFESTANS
EFFECTOR
topic AUTOPHAGY
IMMUNITY
PHYTOPHTHORA INFESTANS
EFFECTOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv During plant cell invasion, the oomycete Phytophthora infestans remains enveloped by host-derived membranes whose functional properties are poorly understood. P. infestans secretes a myriad of effector proteins through these interfaces for plant colonization. Recently we showed that the effector protein PexRD54 reprograms host-selective autophagy by antagonising antimicrobial-autophagy receptor Joka2/NBR1 for ATG8CL binding (Dagdas et al., 2016). Here, we show that during infection, ATG8CL/Joka2 labelled defense-related autophagosomes are diverted toward the perimicrobial host membrane to restrict pathogen growth. PexRD54 also localizes to autophagosomes across the perimicrobial membrane, consistent with the view that the pathogen remodels host-microbe interface by co-opting the host autophagy machinery. Furthermore, we show that the host-pathogen interface is a hotspot for autophagosome biogenesis. Notably, overexpression of the early autophagosome biogenesis protein ATG9 enhances plant immunity. Our results implicate selective autophagy in polarized immune responses of plants and point to more complex functions for autophagy than the widely known degradative roles.
Fil: Dagdas, Yasin F.. Norwich Research Park; Reino Unido. Vienna Biocenter; Austria
Fil: Pandey, Pooja. Imperial College London; Reino Unido
Fil: Tumtas, Yasin. Imperial College London; Reino Unido
Fil: Sanguankiattichai, Nattapong. Imperial College London; Reino Unido
Fil: Belhaj, Khaoula. Norwich Research Park; Reino Unido
Fil: Duggan, Cian. Imperial College London; Reino Unido
Fil: Leary, Alexandre Y.. Imperial College London; Reino Unido
Fil: Segretin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Contreras, Mauricio P.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Imperial College London; Reino Unido
Fil: Savage, Zachary. Imperial College London; Reino Unido
Fil: Khandare, Virendrasinh S.. Imperial College London; Reino Unido
Fil: Kamoun, Sophien. Norwich Research Park; Reino Unido
Fil: Bozkurt, Osman Tolga. Imperial College London; Reino Unido
description During plant cell invasion, the oomycete Phytophthora infestans remains enveloped by host-derived membranes whose functional properties are poorly understood. P. infestans secretes a myriad of effector proteins through these interfaces for plant colonization. Recently we showed that the effector protein PexRD54 reprograms host-selective autophagy by antagonising antimicrobial-autophagy receptor Joka2/NBR1 for ATG8CL binding (Dagdas et al., 2016). Here, we show that during infection, ATG8CL/Joka2 labelled defense-related autophagosomes are diverted toward the perimicrobial host membrane to restrict pathogen growth. PexRD54 also localizes to autophagosomes across the perimicrobial membrane, consistent with the view that the pathogen remodels host-microbe interface by co-opting the host autophagy machinery. Furthermore, we show that the host-pathogen interface is a hotspot for autophagosome biogenesis. Notably, overexpression of the early autophagosome biogenesis protein ATG9 enhances plant immunity. Our results implicate selective autophagy in polarized immune responses of plants and point to more complex functions for autophagy than the widely known degradative roles.
publishDate 2018
dc.date.none.fl_str_mv 2018-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79890
Dagdas, Yasin F.; Pandey, Pooja; Tumtas, Yasin; Sanguankiattichai, Nattapong; Belhaj, Khaoula; et al.; Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the irish potato famine pathogen; eLife Sciences Publications Ltd; eLife; 7; 6-2018; 1-15
2050-084X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79890
identifier_str_mv Dagdas, Yasin F.; Pandey, Pooja; Tumtas, Yasin; Sanguankiattichai, Nattapong; Belhaj, Khaoula; et al.; Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the irish potato famine pathogen; eLife Sciences Publications Ltd; eLife; 7; 6-2018; 1-15
2050-084X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.37476
info:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/37476
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv eLife Sciences Publications Ltd
publisher.none.fl_str_mv eLife Sciences Publications Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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