Nitosylation: an adverse factor in Hemolytic Uremic Syndrome. Antitoxin effect of Ziziphus mistol Griseb

Autores
Aiassa, Virginia; Albrecht, Claudia; Bustos, Pamela Soledad; Ortega, Gabriela Alicia; Eraso, Alberto Jorge; Albesa, Inés
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Toxins of Escherichia coli (STEC) causing Uremic Hemolytic Syndrome (UHS) generate oxidative stress in human blood with more production of nitric oxide (NO) than reactive oxygen species (ROS). Shiga toxin (Stx) together with the hemolysin (Hly) increased lipid oxidation, as evaluated by malondialdehyde MDA and oxidation of proteins. The addition of Ziziphus mistol Griseb extracts decreased NO, ROS, MDA and simultaneously caused an increase in the degradation of oxidized proteins to advanced oxidation protein products (AOPPs) in controls and samples with toxins. Furthermore, the nitrosylated proteins/AOPP ratio was reduced, due to the increase of AOPP. Z. mistol Griseb extracts exhibited a high proportion of polyphenols and flavonoids, with evident correlation with ferrous reduction antioxidant potential (FRAP). The plasma of eight children with UHS showed oxidative stress and NO stimulus, comparable to the effect of toxins during the assays in vitro. UHS children presented high levels of nitrosylated proteins respect to control children of similar age. Although the degradation of oxidized proteins to AOPP rose in UHS children, the nitrosylated proteins/AOPP rate increased as a consequence of the elevated nitrosative stress observed in these patients.
Fil: Aiassa, Virginia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Albrecht, Claudia. Universidad Nacional de Cordoba. Facultad de Medicina. Escuela de Nutrición; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bustos, Pamela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Ortega, Gabriela Alicia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Eraso, Alberto Jorge. Universidad Nacional de Cordoba. Facultad de Medicina. Escuela de Nutrición; Argentina
Fil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Materia
Uremic Hemolytic Syndrome
Escherichia Coli
Ziziphus Mistol Griseb
Nitrosylation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22774
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/22774
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nitosylation: an adverse factor in Hemolytic Uremic Syndrome. Antitoxin effect of Ziziphus mistol GrisebAiassa, VirginiaAlbrecht, ClaudiaBustos, Pamela SoledadOrtega, Gabriela AliciaEraso, Alberto JorgeAlbesa, InésUremic Hemolytic SyndromeEscherichia ColiZiziphus Mistol GrisebNitrosylationhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Toxins of Escherichia coli (STEC) causing Uremic Hemolytic Syndrome (UHS) generate oxidative stress in human blood with more production of nitric oxide (NO) than reactive oxygen species (ROS). Shiga toxin (Stx) together with the hemolysin (Hly) increased lipid oxidation, as evaluated by malondialdehyde MDA and oxidation of proteins. The addition of Ziziphus mistol Griseb extracts decreased NO, ROS, MDA and simultaneously caused an increase in the degradation of oxidized proteins to advanced oxidation protein products (AOPPs) in controls and samples with toxins. Furthermore, the nitrosylated proteins/AOPP ratio was reduced, due to the increase of AOPP. Z. mistol Griseb extracts exhibited a high proportion of polyphenols and flavonoids, with evident correlation with ferrous reduction antioxidant potential (FRAP). The plasma of eight children with UHS showed oxidative stress and NO stimulus, comparable to the effect of toxins during the assays in vitro. UHS children presented high levels of nitrosylated proteins respect to control children of similar age. Although the degradation of oxidized proteins to AOPP rose in UHS children, the nitrosylated proteins/AOPP rate increased as a consequence of the elevated nitrosative stress observed in these patients.Fil: Aiassa, Virginia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Albrecht, Claudia. Universidad Nacional de Cordoba. Facultad de Medicina. Escuela de Nutrición; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bustos, Pamela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Ortega, Gabriela Alicia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaFil: Eraso, Alberto Jorge. Universidad Nacional de Cordoba. Facultad de Medicina. Escuela de Nutrición; ArgentinaFil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaElsevier2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22774Aiassa, Virginia; Albrecht, Claudia; Bustos, Pamela Soledad; Ortega, Gabriela Alicia; Eraso, Alberto Jorge; et al.; Nitosylation: an adverse factor in Hemolytic Uremic Syndrome. Antitoxin effect of Ziziphus mistol Griseb; Elsevier; Food And Chemical Toxicology; 56; 2-2013; 381-3860278-6915CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0278691513001452info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fct.2013.02.036info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:57Zoai:ri.conicet.gov.ar:11336/22774instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:57.447CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nitosylation: an adverse factor in Hemolytic Uremic Syndrome. Antitoxin effect of Ziziphus mistol Griseb
title Nitosylation: an adverse factor in Hemolytic Uremic Syndrome. Antitoxin effect of Ziziphus mistol Griseb
spellingShingle Nitosylation: an adverse factor in Hemolytic Uremic Syndrome. Antitoxin effect of Ziziphus mistol Griseb
Aiassa, Virginia
Uremic Hemolytic Syndrome
Escherichia Coli
Ziziphus Mistol Griseb
Nitrosylation
title_short Nitosylation: an adverse factor in Hemolytic Uremic Syndrome. Antitoxin effect of Ziziphus mistol Griseb
title_full Nitosylation: an adverse factor in Hemolytic Uremic Syndrome. Antitoxin effect of Ziziphus mistol Griseb
title_fullStr Nitosylation: an adverse factor in Hemolytic Uremic Syndrome. Antitoxin effect of Ziziphus mistol Griseb
title_full_unstemmed Nitosylation: an adverse factor in Hemolytic Uremic Syndrome. Antitoxin effect of Ziziphus mistol Griseb
title_sort Nitosylation: an adverse factor in Hemolytic Uremic Syndrome. Antitoxin effect of Ziziphus mistol Griseb
dc.creator.none.fl_str_mv Aiassa, Virginia
Albrecht, Claudia
Bustos, Pamela Soledad
Ortega, Gabriela Alicia
Eraso, Alberto Jorge
Albesa, Inés
author Aiassa, Virginia
author_facet Aiassa, Virginia
Albrecht, Claudia
Bustos, Pamela Soledad
Ortega, Gabriela Alicia
Eraso, Alberto Jorge
Albesa, Inés
author_role author
author2 Albrecht, Claudia
Bustos, Pamela Soledad
Ortega, Gabriela Alicia
Eraso, Alberto Jorge
Albesa, Inés
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Uremic Hemolytic Syndrome
Escherichia Coli
Ziziphus Mistol Griseb
Nitrosylation
topic Uremic Hemolytic Syndrome
Escherichia Coli
Ziziphus Mistol Griseb
Nitrosylation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Toxins of Escherichia coli (STEC) causing Uremic Hemolytic Syndrome (UHS) generate oxidative stress in human blood with more production of nitric oxide (NO) than reactive oxygen species (ROS). Shiga toxin (Stx) together with the hemolysin (Hly) increased lipid oxidation, as evaluated by malondialdehyde MDA and oxidation of proteins. The addition of Ziziphus mistol Griseb extracts decreased NO, ROS, MDA and simultaneously caused an increase in the degradation of oxidized proteins to advanced oxidation protein products (AOPPs) in controls and samples with toxins. Furthermore, the nitrosylated proteins/AOPP ratio was reduced, due to the increase of AOPP. Z. mistol Griseb extracts exhibited a high proportion of polyphenols and flavonoids, with evident correlation with ferrous reduction antioxidant potential (FRAP). The plasma of eight children with UHS showed oxidative stress and NO stimulus, comparable to the effect of toxins during the assays in vitro. UHS children presented high levels of nitrosylated proteins respect to control children of similar age. Although the degradation of oxidized proteins to AOPP rose in UHS children, the nitrosylated proteins/AOPP rate increased as a consequence of the elevated nitrosative stress observed in these patients.
Fil: Aiassa, Virginia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Albrecht, Claudia. Universidad Nacional de Cordoba. Facultad de Medicina. Escuela de Nutrición; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bustos, Pamela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Ortega, Gabriela Alicia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Eraso, Alberto Jorge. Universidad Nacional de Cordoba. Facultad de Medicina. Escuela de Nutrición; Argentina
Fil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
description Toxins of Escherichia coli (STEC) causing Uremic Hemolytic Syndrome (UHS) generate oxidative stress in human blood with more production of nitric oxide (NO) than reactive oxygen species (ROS). Shiga toxin (Stx) together with the hemolysin (Hly) increased lipid oxidation, as evaluated by malondialdehyde MDA and oxidation of proteins. The addition of Ziziphus mistol Griseb extracts decreased NO, ROS, MDA and simultaneously caused an increase in the degradation of oxidized proteins to advanced oxidation protein products (AOPPs) in controls and samples with toxins. Furthermore, the nitrosylated proteins/AOPP ratio was reduced, due to the increase of AOPP. Z. mistol Griseb extracts exhibited a high proportion of polyphenols and flavonoids, with evident correlation with ferrous reduction antioxidant potential (FRAP). The plasma of eight children with UHS showed oxidative stress and NO stimulus, comparable to the effect of toxins during the assays in vitro. UHS children presented high levels of nitrosylated proteins respect to control children of similar age. Although the degradation of oxidized proteins to AOPP rose in UHS children, the nitrosylated proteins/AOPP rate increased as a consequence of the elevated nitrosative stress observed in these patients.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22774
Aiassa, Virginia; Albrecht, Claudia; Bustos, Pamela Soledad; Ortega, Gabriela Alicia; Eraso, Alberto Jorge; et al.; Nitosylation: an adverse factor in Hemolytic Uremic Syndrome. Antitoxin effect of Ziziphus mistol Griseb; Elsevier; Food And Chemical Toxicology; 56; 2-2013; 381-386
0278-6915
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22774
identifier_str_mv Aiassa, Virginia; Albrecht, Claudia; Bustos, Pamela Soledad; Ortega, Gabriela Alicia; Eraso, Alberto Jorge; et al.; Nitosylation: an adverse factor in Hemolytic Uremic Syndrome. Antitoxin effect of Ziziphus mistol Griseb; Elsevier; Food And Chemical Toxicology; 56; 2-2013; 381-386
0278-6915
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0278691513001452
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fct.2013.02.036
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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