Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts
- Autores
- Pellarin, Maria Gabriela; Albrecht, Claudia; Rojas, M. J.; Aguilar, Juan Javier; Konigheim, Brenda Salome; Paraje, María Gabriela; Albesa, Inés; Eraso, Alberto Jorge
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The capacity of Prosopis alba Griseb. and Ziziphus mistol Griseb. fruit extracts to inhibit the toxic action of Shiga toxin (Stx) was investigated. Purification of Stx from Escherichia coli O157:H7 was performed by saline precipitation and affinity chromatography using a column with globotriaosylceramide, while the fruits were subjected to ethanolic or aqueous extractions. The protective action of both fruits was determined by pre-, co-, and postincubation of one 50% cytotoxic dose per ml of Stx with different concentrations of ethanolic and aqueous extracts in confluent monolayers of Vero cells for 72 h at 37°C (5% CO2). The inhibition of the cytotoxic effect of Stx by fruit extracts was determined by the neutral red vital staining technique. The extraction of the polyphenols and flavonoids was effective, and more polyphenols per milligram of dissolved solids were obtained from P. alba than from Z. mistol. However, there were more flavonoids in Z. mistol than in P. alba. Components of both fruits increased the viability of cells treated with Stx when the extracts were preincubated with Stx for 1 h before being applied to the cell cultures, with the ethanolic extract of P. alba showing 95% cell viability at a concentration of 2.45 μg/ml. The extracts were less effective in protecting cells when Stx, extracts, and cells were coincubated together without a previous incubation of Stx; only the concentrations of 19.46 μg/ml for the P. alba aqueous extract and 3.75 μg/ml for the Z. mistol ethanolic extract resulted in the inhibition of cytotoxicity, with 52 and 56% cell viability occurring, respectively. Investigation into this difference in the protection of cells indicated that the protein molecule of Stx suffered degradation to advanced oxidative protein products during preincubation with extracts, principally with P. alba, which exhibited a greater amount of nonflavonoid polyphenols than Z. mistol. The prooxidant action on Stx favored the cells and enhanced the protective action of both fruits.
Fil: Pellarin, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Albrecht, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Rojas, M. J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Aguilar, Juan Javier. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina
Fil: Konigheim, Brenda Salome. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Paraje, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Eraso, Alberto Jorge. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina - Materia
-
Shiga Toxin
Cytotoxicity
Prosopis Alba Griseb
Ziziphus Mistol
Antioxidant Effects - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/84515
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Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extractsPellarin, Maria GabrielaAlbrecht, ClaudiaRojas, M. J.Aguilar, Juan JavierKonigheim, Brenda SalomeParaje, María GabrielaAlbesa, InésEraso, Alberto JorgeShiga ToxinCytotoxicityProsopis Alba GrisebZiziphus MistolAntioxidant Effectshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The capacity of Prosopis alba Griseb. and Ziziphus mistol Griseb. fruit extracts to inhibit the toxic action of Shiga toxin (Stx) was investigated. Purification of Stx from Escherichia coli O157:H7 was performed by saline precipitation and affinity chromatography using a column with globotriaosylceramide, while the fruits were subjected to ethanolic or aqueous extractions. The protective action of both fruits was determined by pre-, co-, and postincubation of one 50% cytotoxic dose per ml of Stx with different concentrations of ethanolic and aqueous extracts in confluent monolayers of Vero cells for 72 h at 37°C (5% CO2). The inhibition of the cytotoxic effect of Stx by fruit extracts was determined by the neutral red vital staining technique. The extraction of the polyphenols and flavonoids was effective, and more polyphenols per milligram of dissolved solids were obtained from P. alba than from Z. mistol. However, there were more flavonoids in Z. mistol than in P. alba. Components of both fruits increased the viability of cells treated with Stx when the extracts were preincubated with Stx for 1 h before being applied to the cell cultures, with the ethanolic extract of P. alba showing 95% cell viability at a concentration of 2.45 μg/ml. The extracts were less effective in protecting cells when Stx, extracts, and cells were coincubated together without a previous incubation of Stx; only the concentrations of 19.46 μg/ml for the P. alba aqueous extract and 3.75 μg/ml for the Z. mistol ethanolic extract resulted in the inhibition of cytotoxicity, with 52 and 56% cell viability occurring, respectively. Investigation into this difference in the protection of cells indicated that the protein molecule of Stx suffered degradation to advanced oxidative protein products during preincubation with extracts, principally with P. alba, which exhibited a greater amount of nonflavonoid polyphenols than Z. mistol. The prooxidant action on Stx favored the cells and enhanced the protective action of both fruits.Fil: Pellarin, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina; ArgentinaFil: Albrecht, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina; ArgentinaFil: Rojas, M. J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina; ArgentinaFil: Aguilar, Juan Javier. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; ArgentinaFil: Konigheim, Brenda Salome. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Paraje, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaFil: Eraso, Alberto Jorge. Universidad Nacional de Córdoba. Facultad de Medicina; ArgentinaInternational Association for Food Protection2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84515Pellarin, Maria Gabriela; Albrecht, Claudia; Rojas, M. J.; Aguilar, Juan Javier; Konigheim, Brenda Salome; et al.; Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts; International Association for Food Protection; Journal of Food Protection; 76; 10; 10-2013; 1733-17390362-028XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/24112573info:eu-repo/semantics/altIdentifier/doi/10.4315/0362-028X.JFP-13-087info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:21Zoai:ri.conicet.gov.ar:11336/84515instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:22.066CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts |
title |
Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts |
spellingShingle |
Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts Pellarin, Maria Gabriela Shiga Toxin Cytotoxicity Prosopis Alba Griseb Ziziphus Mistol Antioxidant Effects |
title_short |
Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts |
title_full |
Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts |
title_fullStr |
Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts |
title_full_unstemmed |
Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts |
title_sort |
Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts |
dc.creator.none.fl_str_mv |
Pellarin, Maria Gabriela Albrecht, Claudia Rojas, M. J. Aguilar, Juan Javier Konigheim, Brenda Salome Paraje, María Gabriela Albesa, Inés Eraso, Alberto Jorge |
author |
Pellarin, Maria Gabriela |
author_facet |
Pellarin, Maria Gabriela Albrecht, Claudia Rojas, M. J. Aguilar, Juan Javier Konigheim, Brenda Salome Paraje, María Gabriela Albesa, Inés Eraso, Alberto Jorge |
author_role |
author |
author2 |
Albrecht, Claudia Rojas, M. J. Aguilar, Juan Javier Konigheim, Brenda Salome Paraje, María Gabriela Albesa, Inés Eraso, Alberto Jorge |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Shiga Toxin Cytotoxicity Prosopis Alba Griseb Ziziphus Mistol Antioxidant Effects |
topic |
Shiga Toxin Cytotoxicity Prosopis Alba Griseb Ziziphus Mistol Antioxidant Effects |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
The capacity of Prosopis alba Griseb. and Ziziphus mistol Griseb. fruit extracts to inhibit the toxic action of Shiga toxin (Stx) was investigated. Purification of Stx from Escherichia coli O157:H7 was performed by saline precipitation and affinity chromatography using a column with globotriaosylceramide, while the fruits were subjected to ethanolic or aqueous extractions. The protective action of both fruits was determined by pre-, co-, and postincubation of one 50% cytotoxic dose per ml of Stx with different concentrations of ethanolic and aqueous extracts in confluent monolayers of Vero cells for 72 h at 37°C (5% CO2). The inhibition of the cytotoxic effect of Stx by fruit extracts was determined by the neutral red vital staining technique. The extraction of the polyphenols and flavonoids was effective, and more polyphenols per milligram of dissolved solids were obtained from P. alba than from Z. mistol. However, there were more flavonoids in Z. mistol than in P. alba. Components of both fruits increased the viability of cells treated with Stx when the extracts were preincubated with Stx for 1 h before being applied to the cell cultures, with the ethanolic extract of P. alba showing 95% cell viability at a concentration of 2.45 μg/ml. The extracts were less effective in protecting cells when Stx, extracts, and cells were coincubated together without a previous incubation of Stx; only the concentrations of 19.46 μg/ml for the P. alba aqueous extract and 3.75 μg/ml for the Z. mistol ethanolic extract resulted in the inhibition of cytotoxicity, with 52 and 56% cell viability occurring, respectively. Investigation into this difference in the protection of cells indicated that the protein molecule of Stx suffered degradation to advanced oxidative protein products during preincubation with extracts, principally with P. alba, which exhibited a greater amount of nonflavonoid polyphenols than Z. mistol. The prooxidant action on Stx favored the cells and enhanced the protective action of both fruits. Fil: Pellarin, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina Fil: Albrecht, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina Fil: Rojas, M. J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina Fil: Aguilar, Juan Javier. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina Fil: Konigheim, Brenda Salome. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Paraje, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina Fil: Eraso, Alberto Jorge. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina |
description |
The capacity of Prosopis alba Griseb. and Ziziphus mistol Griseb. fruit extracts to inhibit the toxic action of Shiga toxin (Stx) was investigated. Purification of Stx from Escherichia coli O157:H7 was performed by saline precipitation and affinity chromatography using a column with globotriaosylceramide, while the fruits were subjected to ethanolic or aqueous extractions. The protective action of both fruits was determined by pre-, co-, and postincubation of one 50% cytotoxic dose per ml of Stx with different concentrations of ethanolic and aqueous extracts in confluent monolayers of Vero cells for 72 h at 37°C (5% CO2). The inhibition of the cytotoxic effect of Stx by fruit extracts was determined by the neutral red vital staining technique. The extraction of the polyphenols and flavonoids was effective, and more polyphenols per milligram of dissolved solids were obtained from P. alba than from Z. mistol. However, there were more flavonoids in Z. mistol than in P. alba. Components of both fruits increased the viability of cells treated with Stx when the extracts were preincubated with Stx for 1 h before being applied to the cell cultures, with the ethanolic extract of P. alba showing 95% cell viability at a concentration of 2.45 μg/ml. The extracts were less effective in protecting cells when Stx, extracts, and cells were coincubated together without a previous incubation of Stx; only the concentrations of 19.46 μg/ml for the P. alba aqueous extract and 3.75 μg/ml for the Z. mistol ethanolic extract resulted in the inhibition of cytotoxicity, with 52 and 56% cell viability occurring, respectively. Investigation into this difference in the protection of cells indicated that the protein molecule of Stx suffered degradation to advanced oxidative protein products during preincubation with extracts, principally with P. alba, which exhibited a greater amount of nonflavonoid polyphenols than Z. mistol. The prooxidant action on Stx favored the cells and enhanced the protective action of both fruits. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/84515 Pellarin, Maria Gabriela; Albrecht, Claudia; Rojas, M. J.; Aguilar, Juan Javier; Konigheim, Brenda Salome; et al.; Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts; International Association for Food Protection; Journal of Food Protection; 76; 10; 10-2013; 1733-1739 0362-028X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/84515 |
identifier_str_mv |
Pellarin, Maria Gabriela; Albrecht, Claudia; Rojas, M. J.; Aguilar, Juan Javier; Konigheim, Brenda Salome; et al.; Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts; International Association for Food Protection; Journal of Food Protection; 76; 10; 10-2013; 1733-1739 0362-028X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/24112573 info:eu-repo/semantics/altIdentifier/doi/10.4315/0362-028X.JFP-13-087 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
International Association for Food Protection |
publisher.none.fl_str_mv |
International Association for Food Protection |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269340373614592 |
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13.13397 |