Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts

Autores
Pellarin, Maria Gabriela; Albrecht, Claudia; Rojas, M. J.; Aguilar, Juan Javier; Konigheim, Brenda Salome; Paraje, María Gabriela; Albesa, Inés; Eraso, Alberto Jorge
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The capacity of Prosopis alba Griseb. and Ziziphus mistol Griseb. fruit extracts to inhibit the toxic action of Shiga toxin (Stx) was investigated. Purification of Stx from Escherichia coli O157:H7 was performed by saline precipitation and affinity chromatography using a column with globotriaosylceramide, while the fruits were subjected to ethanolic or aqueous extractions. The protective action of both fruits was determined by pre-, co-, and postincubation of one 50% cytotoxic dose per ml of Stx with different concentrations of ethanolic and aqueous extracts in confluent monolayers of Vero cells for 72 h at 37°C (5% CO2). The inhibition of the cytotoxic effect of Stx by fruit extracts was determined by the neutral red vital staining technique. The extraction of the polyphenols and flavonoids was effective, and more polyphenols per milligram of dissolved solids were obtained from P. alba than from Z. mistol. However, there were more flavonoids in Z. mistol than in P. alba. Components of both fruits increased the viability of cells treated with Stx when the extracts were preincubated with Stx for 1 h before being applied to the cell cultures, with the ethanolic extract of P. alba showing 95% cell viability at a concentration of 2.45 μg/ml. The extracts were less effective in protecting cells when Stx, extracts, and cells were coincubated together without a previous incubation of Stx; only the concentrations of 19.46 μg/ml for the P. alba aqueous extract and 3.75 μg/ml for the Z. mistol ethanolic extract resulted in the inhibition of cytotoxicity, with 52 and 56% cell viability occurring, respectively. Investigation into this difference in the protection of cells indicated that the protein molecule of Stx suffered degradation to advanced oxidative protein products during preincubation with extracts, principally with P. alba, which exhibited a greater amount of nonflavonoid polyphenols than Z. mistol. The prooxidant action on Stx favored the cells and enhanced the protective action of both fruits.
Fil: Pellarin, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Albrecht, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Rojas, M. J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Aguilar, Juan Javier. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina
Fil: Konigheim, Brenda Salome. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Paraje, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Eraso, Alberto Jorge. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Materia
Shiga Toxin
Cytotoxicity
Prosopis Alba Griseb
Ziziphus Mistol
Antioxidant Effects
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84515
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/84515
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extractsPellarin, Maria GabrielaAlbrecht, ClaudiaRojas, M. J.Aguilar, Juan JavierKonigheim, Brenda SalomeParaje, María GabrielaAlbesa, InésEraso, Alberto JorgeShiga ToxinCytotoxicityProsopis Alba GrisebZiziphus MistolAntioxidant Effectshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The capacity of Prosopis alba Griseb. and Ziziphus mistol Griseb. fruit extracts to inhibit the toxic action of Shiga toxin (Stx) was investigated. Purification of Stx from Escherichia coli O157:H7 was performed by saline precipitation and affinity chromatography using a column with globotriaosylceramide, while the fruits were subjected to ethanolic or aqueous extractions. The protective action of both fruits was determined by pre-, co-, and postincubation of one 50% cytotoxic dose per ml of Stx with different concentrations of ethanolic and aqueous extracts in confluent monolayers of Vero cells for 72 h at 37°C (5% CO2). The inhibition of the cytotoxic effect of Stx by fruit extracts was determined by the neutral red vital staining technique. The extraction of the polyphenols and flavonoids was effective, and more polyphenols per milligram of dissolved solids were obtained from P. alba than from Z. mistol. However, there were more flavonoids in Z. mistol than in P. alba. Components of both fruits increased the viability of cells treated with Stx when the extracts were preincubated with Stx for 1 h before being applied to the cell cultures, with the ethanolic extract of P. alba showing 95% cell viability at a concentration of 2.45 μg/ml. The extracts were less effective in protecting cells when Stx, extracts, and cells were coincubated together without a previous incubation of Stx; only the concentrations of 19.46 μg/ml for the P. alba aqueous extract and 3.75 μg/ml for the Z. mistol ethanolic extract resulted in the inhibition of cytotoxicity, with 52 and 56% cell viability occurring, respectively. Investigation into this difference in the protection of cells indicated that the protein molecule of Stx suffered degradation to advanced oxidative protein products during preincubation with extracts, principally with P. alba, which exhibited a greater amount of nonflavonoid polyphenols than Z. mistol. The prooxidant action on Stx favored the cells and enhanced the protective action of both fruits.Fil: Pellarin, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina; ArgentinaFil: Albrecht, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina; ArgentinaFil: Rojas, M. J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina; ArgentinaFil: Aguilar, Juan Javier. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; ArgentinaFil: Konigheim, Brenda Salome. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Paraje, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaFil: Eraso, Alberto Jorge. Universidad Nacional de Córdoba. Facultad de Medicina; ArgentinaInternational Association for Food Protection2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84515Pellarin, Maria Gabriela; Albrecht, Claudia; Rojas, M. J.; Aguilar, Juan Javier; Konigheim, Brenda Salome; et al.; Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts; International Association for Food Protection; Journal of Food Protection; 76; 10; 10-2013; 1733-17390362-028XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/24112573info:eu-repo/semantics/altIdentifier/doi/10.4315/0362-028X.JFP-13-087info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:57:52Zoai:ri.conicet.gov.ar:11336/84515instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:57:52.392CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts
title Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts
spellingShingle Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts
Pellarin, Maria Gabriela
Shiga Toxin
Cytotoxicity
Prosopis Alba Griseb
Ziziphus Mistol
Antioxidant Effects
title_short Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts
title_full Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts
title_fullStr Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts
title_full_unstemmed Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts
title_sort Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts
dc.creator.none.fl_str_mv Pellarin, Maria Gabriela
Albrecht, Claudia
Rojas, M. J.
Aguilar, Juan Javier
Konigheim, Brenda Salome
Paraje, María Gabriela
Albesa, Inés
Eraso, Alberto Jorge
author Pellarin, Maria Gabriela
author_facet Pellarin, Maria Gabriela
Albrecht, Claudia
Rojas, M. J.
Aguilar, Juan Javier
Konigheim, Brenda Salome
Paraje, María Gabriela
Albesa, Inés
Eraso, Alberto Jorge
author_role author
author2 Albrecht, Claudia
Rojas, M. J.
Aguilar, Juan Javier
Konigheim, Brenda Salome
Paraje, María Gabriela
Albesa, Inés
Eraso, Alberto Jorge
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Shiga Toxin
Cytotoxicity
Prosopis Alba Griseb
Ziziphus Mistol
Antioxidant Effects
topic Shiga Toxin
Cytotoxicity
Prosopis Alba Griseb
Ziziphus Mistol
Antioxidant Effects
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The capacity of Prosopis alba Griseb. and Ziziphus mistol Griseb. fruit extracts to inhibit the toxic action of Shiga toxin (Stx) was investigated. Purification of Stx from Escherichia coli O157:H7 was performed by saline precipitation and affinity chromatography using a column with globotriaosylceramide, while the fruits were subjected to ethanolic or aqueous extractions. The protective action of both fruits was determined by pre-, co-, and postincubation of one 50% cytotoxic dose per ml of Stx with different concentrations of ethanolic and aqueous extracts in confluent monolayers of Vero cells for 72 h at 37°C (5% CO2). The inhibition of the cytotoxic effect of Stx by fruit extracts was determined by the neutral red vital staining technique. The extraction of the polyphenols and flavonoids was effective, and more polyphenols per milligram of dissolved solids were obtained from P. alba than from Z. mistol. However, there were more flavonoids in Z. mistol than in P. alba. Components of both fruits increased the viability of cells treated with Stx when the extracts were preincubated with Stx for 1 h before being applied to the cell cultures, with the ethanolic extract of P. alba showing 95% cell viability at a concentration of 2.45 μg/ml. The extracts were less effective in protecting cells when Stx, extracts, and cells were coincubated together without a previous incubation of Stx; only the concentrations of 19.46 μg/ml for the P. alba aqueous extract and 3.75 μg/ml for the Z. mistol ethanolic extract resulted in the inhibition of cytotoxicity, with 52 and 56% cell viability occurring, respectively. Investigation into this difference in the protection of cells indicated that the protein molecule of Stx suffered degradation to advanced oxidative protein products during preincubation with extracts, principally with P. alba, which exhibited a greater amount of nonflavonoid polyphenols than Z. mistol. The prooxidant action on Stx favored the cells and enhanced the protective action of both fruits.
Fil: Pellarin, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Albrecht, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Rojas, M. J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Aguilar, Juan Javier. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina
Fil: Konigheim, Brenda Salome. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Paraje, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Eraso, Alberto Jorge. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
description The capacity of Prosopis alba Griseb. and Ziziphus mistol Griseb. fruit extracts to inhibit the toxic action of Shiga toxin (Stx) was investigated. Purification of Stx from Escherichia coli O157:H7 was performed by saline precipitation and affinity chromatography using a column with globotriaosylceramide, while the fruits were subjected to ethanolic or aqueous extractions. The protective action of both fruits was determined by pre-, co-, and postincubation of one 50% cytotoxic dose per ml of Stx with different concentrations of ethanolic and aqueous extracts in confluent monolayers of Vero cells for 72 h at 37°C (5% CO2). The inhibition of the cytotoxic effect of Stx by fruit extracts was determined by the neutral red vital staining technique. The extraction of the polyphenols and flavonoids was effective, and more polyphenols per milligram of dissolved solids were obtained from P. alba than from Z. mistol. However, there were more flavonoids in Z. mistol than in P. alba. Components of both fruits increased the viability of cells treated with Stx when the extracts were preincubated with Stx for 1 h before being applied to the cell cultures, with the ethanolic extract of P. alba showing 95% cell viability at a concentration of 2.45 μg/ml. The extracts were less effective in protecting cells when Stx, extracts, and cells were coincubated together without a previous incubation of Stx; only the concentrations of 19.46 μg/ml for the P. alba aqueous extract and 3.75 μg/ml for the Z. mistol ethanolic extract resulted in the inhibition of cytotoxicity, with 52 and 56% cell viability occurring, respectively. Investigation into this difference in the protection of cells indicated that the protein molecule of Stx suffered degradation to advanced oxidative protein products during preincubation with extracts, principally with P. alba, which exhibited a greater amount of nonflavonoid polyphenols than Z. mistol. The prooxidant action on Stx favored the cells and enhanced the protective action of both fruits.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84515
Pellarin, Maria Gabriela; Albrecht, Claudia; Rojas, M. J.; Aguilar, Juan Javier; Konigheim, Brenda Salome; et al.; Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts; International Association for Food Protection; Journal of Food Protection; 76; 10; 10-2013; 1733-1739
0362-028X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84515
identifier_str_mv Pellarin, Maria Gabriela; Albrecht, Claudia; Rojas, M. J.; Aguilar, Juan Javier; Konigheim, Brenda Salome; et al.; Inhibition of cytotoxicity of shiga toxin of Escherichia coli O157:H7 on vero cells by Prosopis alba griseb (Fabaceae) and Ziziphus mistol griseb (Rhamnaceae) extracts; International Association for Food Protection; Journal of Food Protection; 76; 10; 10-2013; 1733-1739
0362-028X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/24112573
info:eu-repo/semantics/altIdentifier/doi/10.4315/0362-028X.JFP-13-087
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Association for Food Protection
publisher.none.fl_str_mv International Association for Food Protection
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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