Latex particles by emulsion and dispersion polymerizations: sensitization with specific antigens of leptospirosis and application to immunoagglutination

Autores
Peretti, Leandro Ezequiel; González, Verónica Doris Guadalupe; Clementi, Luis Alberto; Gugliotta, Luis Marcelino
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The controlled synthesis of polymer nanoparticles is of great interest in the production of latexes with well defined characteristics. These products can be applied in biomedicine as carriers of biomolecules (e.g. proteins and enzymes), and in particular as latexes for immunoassays used for example in immunoagglutination test, which allow amplify the antigen–antibody reaction, being simple, quick and inexpensive diagnostic tools. The synthesis of polystyrene (PS) latex particles and of core–shell particles, with controlled size distribution, functional groups and surface charge densities is considered here. PS latexes were synthesized in the 100–1100 nm diameter range, by either emulsion or dispersion polymerization. Such latexes were then used as seeds in emulsion copolymerizations of styrene and a functional monomer (methacrylic acid or glycidyl methacrylate), thus producing particles with carboxyl or epoxy functionalities, respectively. Changes in the polymerization recipes employed under batch operation were analyzed. Latex characterization involved measurements of mean particle diameters, the polydispersity index, functional group densities and zeta potential. Finally, latexes were sensitized (by either physical adsorption or covalent coupling) with specific antigens to obtain latex–protein complexes, and one of them was tested as agglutination assay for detecting leptospirosis disease in bovine samples, as an example of the potential application of the latexes produced.
Fil: Peretti, Leandro Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Clementi, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Gugliotta, Luis Marcelino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Materia
DISPERSION POLYMERIZATION
EMULSION POLYMERIZATION
IMMUNOAGGLUTINATION
LEPTOSPIROSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/127907
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/127907
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Latex particles by emulsion and dispersion polymerizations: sensitization with specific antigens of leptospirosis and application to immunoagglutinationPeretti, Leandro EzequielGonzález, Verónica Doris GuadalupeClementi, Luis AlbertoGugliotta, Luis MarcelinoDISPERSION POLYMERIZATIONEMULSION POLYMERIZATIONIMMUNOAGGLUTINATIONLEPTOSPIROSIShttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2The controlled synthesis of polymer nanoparticles is of great interest in the production of latexes with well defined characteristics. These products can be applied in biomedicine as carriers of biomolecules (e.g. proteins and enzymes), and in particular as latexes for immunoassays used for example in immunoagglutination test, which allow amplify the antigen–antibody reaction, being simple, quick and inexpensive diagnostic tools. The synthesis of polystyrene (PS) latex particles and of core–shell particles, with controlled size distribution, functional groups and surface charge densities is considered here. PS latexes were synthesized in the 100–1100 nm diameter range, by either emulsion or dispersion polymerization. Such latexes were then used as seeds in emulsion copolymerizations of styrene and a functional monomer (methacrylic acid or glycidyl methacrylate), thus producing particles with carboxyl or epoxy functionalities, respectively. Changes in the polymerization recipes employed under batch operation were analyzed. Latex characterization involved measurements of mean particle diameters, the polydispersity index, functional group densities and zeta potential. Finally, latexes were sensitized (by either physical adsorption or covalent coupling) with specific antigens to obtain latex–protein complexes, and one of them was tested as agglutination assay for detecting leptospirosis disease in bovine samples, as an example of the potential application of the latexes produced.Fil: Peretti, Leandro Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Clementi, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Gugliotta, Luis Marcelino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaBrazilian Society of Chemical Engineering2021-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/127907Peretti, Leandro Ezequiel; González, Verónica Doris Guadalupe; Clementi, Luis Alberto; Gugliotta, Luis Marcelino; Latex particles by emulsion and dispersion polymerizations: sensitization with specific antigens of leptospirosis and application to immunoagglutination; Brazilian Society of Chemical Engineering; Brazilian Journal of Chemical Engineering; 38; 1; 3-2021; 145-1540104-66321678-4383CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s43153-020-00080-4info:eu-repo/semantics/altIdentifier/doi/10.1007/s43153-020-00080-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:18:07Zoai:ri.conicet.gov.ar:11336/127907instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:18:08.007CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Latex particles by emulsion and dispersion polymerizations: sensitization with specific antigens of leptospirosis and application to immunoagglutination
title Latex particles by emulsion and dispersion polymerizations: sensitization with specific antigens of leptospirosis and application to immunoagglutination
spellingShingle Latex particles by emulsion and dispersion polymerizations: sensitization with specific antigens of leptospirosis and application to immunoagglutination
Peretti, Leandro Ezequiel
DISPERSION POLYMERIZATION
EMULSION POLYMERIZATION
IMMUNOAGGLUTINATION
LEPTOSPIROSIS
title_short Latex particles by emulsion and dispersion polymerizations: sensitization with specific antigens of leptospirosis and application to immunoagglutination
title_full Latex particles by emulsion and dispersion polymerizations: sensitization with specific antigens of leptospirosis and application to immunoagglutination
title_fullStr Latex particles by emulsion and dispersion polymerizations: sensitization with specific antigens of leptospirosis and application to immunoagglutination
title_full_unstemmed Latex particles by emulsion and dispersion polymerizations: sensitization with specific antigens of leptospirosis and application to immunoagglutination
title_sort Latex particles by emulsion and dispersion polymerizations: sensitization with specific antigens of leptospirosis and application to immunoagglutination
dc.creator.none.fl_str_mv Peretti, Leandro Ezequiel
González, Verónica Doris Guadalupe
Clementi, Luis Alberto
Gugliotta, Luis Marcelino
author Peretti, Leandro Ezequiel
author_facet Peretti, Leandro Ezequiel
González, Verónica Doris Guadalupe
Clementi, Luis Alberto
Gugliotta, Luis Marcelino
author_role author
author2 González, Verónica Doris Guadalupe
Clementi, Luis Alberto
Gugliotta, Luis Marcelino
author2_role author
author
author
dc.subject.none.fl_str_mv DISPERSION POLYMERIZATION
EMULSION POLYMERIZATION
IMMUNOAGGLUTINATION
LEPTOSPIROSIS
topic DISPERSION POLYMERIZATION
EMULSION POLYMERIZATION
IMMUNOAGGLUTINATION
LEPTOSPIROSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The controlled synthesis of polymer nanoparticles is of great interest in the production of latexes with well defined characteristics. These products can be applied in biomedicine as carriers of biomolecules (e.g. proteins and enzymes), and in particular as latexes for immunoassays used for example in immunoagglutination test, which allow amplify the antigen–antibody reaction, being simple, quick and inexpensive diagnostic tools. The synthesis of polystyrene (PS) latex particles and of core–shell particles, with controlled size distribution, functional groups and surface charge densities is considered here. PS latexes were synthesized in the 100–1100 nm diameter range, by either emulsion or dispersion polymerization. Such latexes were then used as seeds in emulsion copolymerizations of styrene and a functional monomer (methacrylic acid or glycidyl methacrylate), thus producing particles with carboxyl or epoxy functionalities, respectively. Changes in the polymerization recipes employed under batch operation were analyzed. Latex characterization involved measurements of mean particle diameters, the polydispersity index, functional group densities and zeta potential. Finally, latexes were sensitized (by either physical adsorption or covalent coupling) with specific antigens to obtain latex–protein complexes, and one of them was tested as agglutination assay for detecting leptospirosis disease in bovine samples, as an example of the potential application of the latexes produced.
Fil: Peretti, Leandro Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Clementi, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Gugliotta, Luis Marcelino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
description The controlled synthesis of polymer nanoparticles is of great interest in the production of latexes with well defined characteristics. These products can be applied in biomedicine as carriers of biomolecules (e.g. proteins and enzymes), and in particular as latexes for immunoassays used for example in immunoagglutination test, which allow amplify the antigen–antibody reaction, being simple, quick and inexpensive diagnostic tools. The synthesis of polystyrene (PS) latex particles and of core–shell particles, with controlled size distribution, functional groups and surface charge densities is considered here. PS latexes were synthesized in the 100–1100 nm diameter range, by either emulsion or dispersion polymerization. Such latexes were then used as seeds in emulsion copolymerizations of styrene and a functional monomer (methacrylic acid or glycidyl methacrylate), thus producing particles with carboxyl or epoxy functionalities, respectively. Changes in the polymerization recipes employed under batch operation were analyzed. Latex characterization involved measurements of mean particle diameters, the polydispersity index, functional group densities and zeta potential. Finally, latexes were sensitized (by either physical adsorption or covalent coupling) with specific antigens to obtain latex–protein complexes, and one of them was tested as agglutination assay for detecting leptospirosis disease in bovine samples, as an example of the potential application of the latexes produced.
publishDate 2021
dc.date.none.fl_str_mv 2021-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/127907
Peretti, Leandro Ezequiel; González, Verónica Doris Guadalupe; Clementi, Luis Alberto; Gugliotta, Luis Marcelino; Latex particles by emulsion and dispersion polymerizations: sensitization with specific antigens of leptospirosis and application to immunoagglutination; Brazilian Society of Chemical Engineering; Brazilian Journal of Chemical Engineering; 38; 1; 3-2021; 145-154
0104-6632
1678-4383
CONICET Digital
CONICET
url http://hdl.handle.net/11336/127907
identifier_str_mv Peretti, Leandro Ezequiel; González, Verónica Doris Guadalupe; Clementi, Luis Alberto; Gugliotta, Luis Marcelino; Latex particles by emulsion and dispersion polymerizations: sensitization with specific antigens of leptospirosis and application to immunoagglutination; Brazilian Society of Chemical Engineering; Brazilian Journal of Chemical Engineering; 38; 1; 3-2021; 145-154
0104-6632
1678-4383
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s43153-020-00080-4
info:eu-repo/semantics/altIdentifier/doi/10.1007/s43153-020-00080-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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