Synthesis and characterization of carboxyl and acetal latexes by emulsion polymerization. Application to the production of latex-protein complexes for detecting chagas disease

Autores
Garcia, Valeria Soledad; Gonzalez, Veronica Doris Guadalupe; Vega, Jorge Ruben; Marcipar, Ivan Sergio; Gugliotta, Luis Marcelino
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Monodisperse polymer particles with carboxyl and acetal functionalities were synthesized through a two-step emulsion polymerization process. In the first step, latex particles were synthesized by batch emulsion polymerization of styrene (St); and in the second step, the functional monomers (methacrylic acid or acrolein diethyl acetal) were copolymerized with St onto the previously formed polystyrene particles. The synthesized “core-shell” latexes were used as support for their sensitization (by covalent coupling) with two antigenic recombinant proteins of Trypanosoma cruzi (RP1 and CP1). Polymer latexes and latex-protein complexes were characterized by measuring their colloidal stability, average particle size, shell thickness and protein thickness through conductimetric titration, dynamic light scattering, turbidimetry and scanning electron microscopy.
Fil: Garcia, Valeria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico Para la Industria Química; Argentina
Fil: Gonzalez, Veronica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico Para la Industria Química; Argentina
Fil: Vega, Jorge Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico Para la Industria Química; Argentina
Fil: Marcipar, Ivan Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Tecnología Biologica; Argentina
Fil: Gugliotta, Luis Marcelino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico Para la Industria Química; Argentina
Materia
Emulsion Polymerization
Functionalized Latex
Recombinant Protein
Chagas Disease
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/18645
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Synthesis and characterization of carboxyl and acetal latexes by emulsion polymerization. Application to the production of latex-protein complexes for detecting chagas diseaseGarcia, Valeria SoledadGonzalez, Veronica Doris GuadalupeVega, Jorge RubenMarcipar, Ivan SergioGugliotta, Luis MarcelinoEmulsion PolymerizationFunctionalized LatexRecombinant ProteinChagas Diseasehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Monodisperse polymer particles with carboxyl and acetal functionalities were synthesized through a two-step emulsion polymerization process. In the first step, latex particles were synthesized by batch emulsion polymerization of styrene (St); and in the second step, the functional monomers (methacrylic acid or acrolein diethyl acetal) were copolymerized with St onto the previously formed polystyrene particles. The synthesized “core-shell” latexes were used as support for their sensitization (by covalent coupling) with two antigenic recombinant proteins of Trypanosoma cruzi (RP1 and CP1). Polymer latexes and latex-protein complexes were characterized by measuring their colloidal stability, average particle size, shell thickness and protein thickness through conductimetric titration, dynamic light scattering, turbidimetry and scanning electron microscopy.Fil: Garcia, Valeria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico Para la Industria Química; ArgentinaFil: Gonzalez, Veronica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico Para la Industria Química; ArgentinaFil: Vega, Jorge Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico Para la Industria Química; ArgentinaFil: Marcipar, Ivan Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Tecnología Biologica; ArgentinaFil: Gugliotta, Luis Marcelino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico Para la Industria Química; ArgentinaPlanta Piloto de Ingeniería Química2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18645Garcia, Valeria Soledad; Gonzalez, Veronica Doris Guadalupe; Vega, Jorge Ruben; Marcipar, Ivan Sergio; Gugliotta, Luis Marcelino; Synthesis and characterization of carboxyl and acetal latexes by emulsion polymerization. Application to the production of latex-protein complexes for detecting chagas disease; Planta Piloto de Ingeniería Química; Latin American Applied Research; 42; 4; 5-2012; 405-4120327-07931851-8796CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/46gc24info:eu-repo/semantics/altIdentifier/url/http://www.laar.uns.edu.ar/indexes/artic_v4204/Vol42_04_405.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:33Zoai:ri.conicet.gov.ar:11336/18645instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:33.448CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Synthesis and characterization of carboxyl and acetal latexes by emulsion polymerization. Application to the production of latex-protein complexes for detecting chagas disease
title Synthesis and characterization of carboxyl and acetal latexes by emulsion polymerization. Application to the production of latex-protein complexes for detecting chagas disease
spellingShingle Synthesis and characterization of carboxyl and acetal latexes by emulsion polymerization. Application to the production of latex-protein complexes for detecting chagas disease
Garcia, Valeria Soledad
Emulsion Polymerization
Functionalized Latex
Recombinant Protein
Chagas Disease
title_short Synthesis and characterization of carboxyl and acetal latexes by emulsion polymerization. Application to the production of latex-protein complexes for detecting chagas disease
title_full Synthesis and characterization of carboxyl and acetal latexes by emulsion polymerization. Application to the production of latex-protein complexes for detecting chagas disease
title_fullStr Synthesis and characterization of carboxyl and acetal latexes by emulsion polymerization. Application to the production of latex-protein complexes for detecting chagas disease
title_full_unstemmed Synthesis and characterization of carboxyl and acetal latexes by emulsion polymerization. Application to the production of latex-protein complexes for detecting chagas disease
title_sort Synthesis and characterization of carboxyl and acetal latexes by emulsion polymerization. Application to the production of latex-protein complexes for detecting chagas disease
dc.creator.none.fl_str_mv Garcia, Valeria Soledad
Gonzalez, Veronica Doris Guadalupe
Vega, Jorge Ruben
Marcipar, Ivan Sergio
Gugliotta, Luis Marcelino
author Garcia, Valeria Soledad
author_facet Garcia, Valeria Soledad
Gonzalez, Veronica Doris Guadalupe
Vega, Jorge Ruben
Marcipar, Ivan Sergio
Gugliotta, Luis Marcelino
author_role author
author2 Gonzalez, Veronica Doris Guadalupe
Vega, Jorge Ruben
Marcipar, Ivan Sergio
Gugliotta, Luis Marcelino
author2_role author
author
author
author
dc.subject.none.fl_str_mv Emulsion Polymerization
Functionalized Latex
Recombinant Protein
Chagas Disease
topic Emulsion Polymerization
Functionalized Latex
Recombinant Protein
Chagas Disease
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Monodisperse polymer particles with carboxyl and acetal functionalities were synthesized through a two-step emulsion polymerization process. In the first step, latex particles were synthesized by batch emulsion polymerization of styrene (St); and in the second step, the functional monomers (methacrylic acid or acrolein diethyl acetal) were copolymerized with St onto the previously formed polystyrene particles. The synthesized “core-shell” latexes were used as support for their sensitization (by covalent coupling) with two antigenic recombinant proteins of Trypanosoma cruzi (RP1 and CP1). Polymer latexes and latex-protein complexes were characterized by measuring their colloidal stability, average particle size, shell thickness and protein thickness through conductimetric titration, dynamic light scattering, turbidimetry and scanning electron microscopy.
Fil: Garcia, Valeria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico Para la Industria Química; Argentina
Fil: Gonzalez, Veronica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico Para la Industria Química; Argentina
Fil: Vega, Jorge Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico Para la Industria Química; Argentina
Fil: Marcipar, Ivan Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Tecnología Biologica; Argentina
Fil: Gugliotta, Luis Marcelino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico Para la Industria Química; Argentina
description Monodisperse polymer particles with carboxyl and acetal functionalities were synthesized through a two-step emulsion polymerization process. In the first step, latex particles were synthesized by batch emulsion polymerization of styrene (St); and in the second step, the functional monomers (methacrylic acid or acrolein diethyl acetal) were copolymerized with St onto the previously formed polystyrene particles. The synthesized “core-shell” latexes were used as support for their sensitization (by covalent coupling) with two antigenic recombinant proteins of Trypanosoma cruzi (RP1 and CP1). Polymer latexes and latex-protein complexes were characterized by measuring their colloidal stability, average particle size, shell thickness and protein thickness through conductimetric titration, dynamic light scattering, turbidimetry and scanning electron microscopy.
publishDate 2012
dc.date.none.fl_str_mv 2012-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18645
Garcia, Valeria Soledad; Gonzalez, Veronica Doris Guadalupe; Vega, Jorge Ruben; Marcipar, Ivan Sergio; Gugliotta, Luis Marcelino; Synthesis and characterization of carboxyl and acetal latexes by emulsion polymerization. Application to the production of latex-protein complexes for detecting chagas disease; Planta Piloto de Ingeniería Química; Latin American Applied Research; 42; 4; 5-2012; 405-412
0327-0793
1851-8796
CONICET Digital
CONICET
url http://hdl.handle.net/11336/18645
identifier_str_mv Garcia, Valeria Soledad; Gonzalez, Veronica Doris Guadalupe; Vega, Jorge Ruben; Marcipar, Ivan Sergio; Gugliotta, Luis Marcelino; Synthesis and characterization of carboxyl and acetal latexes by emulsion polymerization. Application to the production of latex-protein complexes for detecting chagas disease; Planta Piloto de Ingeniería Química; Latin American Applied Research; 42; 4; 5-2012; 405-412
0327-0793
1851-8796
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/46gc24
info:eu-repo/semantics/altIdentifier/url/http://www.laar.uns.edu.ar/indexes/artic_v4204/Vol42_04_405.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Planta Piloto de Ingeniería Química
publisher.none.fl_str_mv Planta Piloto de Ingeniería Química
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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