Immunoagglutination test to diagnose Chagas disease: comparison of different latex-antigen complexes

Autores
Garcia, Valeria Soledad; Gonzalez, Veronica Doris Guadalupe; Marcipar, Ivan Sergio; Gugliotta, Luis Marcelino
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
OBJETIVE To evaluate the diagnostic performance of novel latex–protein complexes obtained from different antigens of Trypanosoma cruzi through immunoagglutination test using a panel of T. cruzipositive sera, leishmaniasis-positive sera and negative sera for both parasites. METHODS Complexes’ behaviour using total parasite homogenate (TPH), two simple recombinant proteins (RP1 and RP5) and two chimeric recombinant proteins (CP1 and CP2) was comparatively evaluated. The area under ROC curves was used as an index of accuracy. Sensitivity, specificity and discrimination efficiency were assessed. RESULTS All recombinant antigens showed higher specificity than TPH. The lower specificity of TPH was mainly due to cross-reacting peptides between T. cruzi and Leishmania spp. In turn, all performance indicators were higher for CP1 and CP2 than for RP1 and RP5. The carboxylated latex– CP2 (C2–CP2) complex was able to detect antibodies against T. cruzi. The values of area under ROC curve (0.96), sensitivity (92.3%, 95% CI: 79.4–100.0%) and specificity (84.0%, 95% CI: 67.6– 100.0%) indicate that the assay could be used as a screening test. conclusion The C2–CP2 complex could be an important tool to carry out sero-epidemiological studies.
Fil: Garcia, Valeria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina
Fil: Gonzalez, Veronica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina
Fil: Marcipar, Ivan Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentina
Fil: Gugliotta, Luis Marcelino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina
Materia
Immunoagglutination
Chagas Disease
Latex-Protein Complexes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/9267
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunoagglutination test to diagnose Chagas disease: comparison of different latex-antigen complexesGarcia, Valeria SoledadGonzalez, Veronica Doris GuadalupeMarcipar, Ivan SergioGugliotta, Luis MarcelinoImmunoagglutinationChagas DiseaseLatex-Protein Complexeshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1OBJETIVE To evaluate the diagnostic performance of novel latex–protein complexes obtained from different antigens of Trypanosoma cruzi through immunoagglutination test using a panel of T. cruzipositive sera, leishmaniasis-positive sera and negative sera for both parasites. METHODS Complexes’ behaviour using total parasite homogenate (TPH), two simple recombinant proteins (RP1 and RP5) and two chimeric recombinant proteins (CP1 and CP2) was comparatively evaluated. The area under ROC curves was used as an index of accuracy. Sensitivity, specificity and discrimination efficiency were assessed. RESULTS All recombinant antigens showed higher specificity than TPH. The lower specificity of TPH was mainly due to cross-reacting peptides between T. cruzi and Leishmania spp. In turn, all performance indicators were higher for CP1 and CP2 than for RP1 and RP5. The carboxylated latex– CP2 (C2–CP2) complex was able to detect antibodies against T. cruzi. The values of area under ROC curve (0.96), sensitivity (92.3%, 95% CI: 79.4–100.0%) and specificity (84.0%, 95% CI: 67.6– 100.0%) indicate that the assay could be used as a screening test. conclusion The C2–CP2 complex could be an important tool to carry out sero-epidemiological studies.Fil: Garcia, Valeria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); ArgentinaFil: Gonzalez, Veronica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); ArgentinaFil: Marcipar, Ivan Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; ArgentinaFil: Gugliotta, Luis Marcelino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); ArgentinaWiley2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/9267Garcia, Valeria Soledad; Gonzalez, Veronica Doris Guadalupe; Marcipar, Ivan Sergio; Gugliotta, Luis Marcelino; Immunoagglutination test to diagnose Chagas disease: comparison of different latex-antigen complexes; Wiley; Tropical Medicine And International Health; 19; 8-2014; 1346-13541360-2276enginfo:eu-repo/semantics/altIdentifier/doi/10.1111/tmi.12379info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/tmi.12379/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:05Zoai:ri.conicet.gov.ar:11336/9267instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:05.96CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunoagglutination test to diagnose Chagas disease: comparison of different latex-antigen complexes
title Immunoagglutination test to diagnose Chagas disease: comparison of different latex-antigen complexes
spellingShingle Immunoagglutination test to diagnose Chagas disease: comparison of different latex-antigen complexes
Garcia, Valeria Soledad
Immunoagglutination
Chagas Disease
Latex-Protein Complexes
title_short Immunoagglutination test to diagnose Chagas disease: comparison of different latex-antigen complexes
title_full Immunoagglutination test to diagnose Chagas disease: comparison of different latex-antigen complexes
title_fullStr Immunoagglutination test to diagnose Chagas disease: comparison of different latex-antigen complexes
title_full_unstemmed Immunoagglutination test to diagnose Chagas disease: comparison of different latex-antigen complexes
title_sort Immunoagglutination test to diagnose Chagas disease: comparison of different latex-antigen complexes
dc.creator.none.fl_str_mv Garcia, Valeria Soledad
Gonzalez, Veronica Doris Guadalupe
Marcipar, Ivan Sergio
Gugliotta, Luis Marcelino
author Garcia, Valeria Soledad
author_facet Garcia, Valeria Soledad
Gonzalez, Veronica Doris Guadalupe
Marcipar, Ivan Sergio
Gugliotta, Luis Marcelino
author_role author
author2 Gonzalez, Veronica Doris Guadalupe
Marcipar, Ivan Sergio
Gugliotta, Luis Marcelino
author2_role author
author
author
dc.subject.none.fl_str_mv Immunoagglutination
Chagas Disease
Latex-Protein Complexes
topic Immunoagglutination
Chagas Disease
Latex-Protein Complexes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv OBJETIVE To evaluate the diagnostic performance of novel latex–protein complexes obtained from different antigens of Trypanosoma cruzi through immunoagglutination test using a panel of T. cruzipositive sera, leishmaniasis-positive sera and negative sera for both parasites. METHODS Complexes’ behaviour using total parasite homogenate (TPH), two simple recombinant proteins (RP1 and RP5) and two chimeric recombinant proteins (CP1 and CP2) was comparatively evaluated. The area under ROC curves was used as an index of accuracy. Sensitivity, specificity and discrimination efficiency were assessed. RESULTS All recombinant antigens showed higher specificity than TPH. The lower specificity of TPH was mainly due to cross-reacting peptides between T. cruzi and Leishmania spp. In turn, all performance indicators were higher for CP1 and CP2 than for RP1 and RP5. The carboxylated latex– CP2 (C2–CP2) complex was able to detect antibodies against T. cruzi. The values of area under ROC curve (0.96), sensitivity (92.3%, 95% CI: 79.4–100.0%) and specificity (84.0%, 95% CI: 67.6– 100.0%) indicate that the assay could be used as a screening test. conclusion The C2–CP2 complex could be an important tool to carry out sero-epidemiological studies.
Fil: Garcia, Valeria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina
Fil: Gonzalez, Veronica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina
Fil: Marcipar, Ivan Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentina
Fil: Gugliotta, Luis Marcelino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina
description OBJETIVE To evaluate the diagnostic performance of novel latex–protein complexes obtained from different antigens of Trypanosoma cruzi through immunoagglutination test using a panel of T. cruzipositive sera, leishmaniasis-positive sera and negative sera for both parasites. METHODS Complexes’ behaviour using total parasite homogenate (TPH), two simple recombinant proteins (RP1 and RP5) and two chimeric recombinant proteins (CP1 and CP2) was comparatively evaluated. The area under ROC curves was used as an index of accuracy. Sensitivity, specificity and discrimination efficiency were assessed. RESULTS All recombinant antigens showed higher specificity than TPH. The lower specificity of TPH was mainly due to cross-reacting peptides between T. cruzi and Leishmania spp. In turn, all performance indicators were higher for CP1 and CP2 than for RP1 and RP5. The carboxylated latex– CP2 (C2–CP2) complex was able to detect antibodies against T. cruzi. The values of area under ROC curve (0.96), sensitivity (92.3%, 95% CI: 79.4–100.0%) and specificity (84.0%, 95% CI: 67.6– 100.0%) indicate that the assay could be used as a screening test. conclusion The C2–CP2 complex could be an important tool to carry out sero-epidemiological studies.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/9267
Garcia, Valeria Soledad; Gonzalez, Veronica Doris Guadalupe; Marcipar, Ivan Sergio; Gugliotta, Luis Marcelino; Immunoagglutination test to diagnose Chagas disease: comparison of different latex-antigen complexes; Wiley; Tropical Medicine And International Health; 19; 8-2014; 1346-1354
1360-2276
url http://hdl.handle.net/11336/9267
identifier_str_mv Garcia, Valeria Soledad; Gonzalez, Veronica Doris Guadalupe; Marcipar, Ivan Sergio; Gugliotta, Luis Marcelino; Immunoagglutination test to diagnose Chagas disease: comparison of different latex-antigen complexes; Wiley; Tropical Medicine And International Health; 19; 8-2014; 1346-1354
1360-2276
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/tmi.12379
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/tmi.12379/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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