Low-density membrane fractions from male germ cells lack sphingolipids with very long chain PUFA

Autores
Santiago Valtierra, Florencia Ximena; Mateos, Melina Valeria; Aveldaño, Marta Isabel; Oresti, Gerardo Martin
Año de publicación
2016
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Sphingomyelins (SM) and ceramides (Cer) with very long-chain PUFA (VLCPUFA), in nonhydroxy (n-V) and 2-hydroxy (h-V) forms, are specific components of rat spermatogenic cells. Here we evaluated how differentiation affects their distribution among membrane fractions from such cells. Using a detergent-free procedure, a small light, raft-like low-density (L) fraction and a large heavier (H) fraction, both showing markers typical of cell plasma membranes, were separated from pachytene spermatocytes, round, and late spermatids. MALDI-TOF spectra showed that the L fraction had mostly SM species with saturated fatty acids regardless of the cell stage, while the H fraction was rich in stage-varying SM and Cer species with VLCPUFA. In this fraction spermatocytes accumulated mostly n-V SM and spermatids h-V SM and h-V Cer species. A third fraction made of intracellular membranes had less SM and more Cer than the H fraction, differentiation also increasing the h-V/n-V ratio in both lipids. The buildup of 2-hydroxy fatty acids correlated with the expression (mRNA) of fatty acid 2-hydroxylase (Fa2h), higher in spermatids than in spermatocytes. The differentiation-dependent rise in h-V Cer in the germ cell H fraction during spermatogenesis is consistent with the eventually uneven distribution that n-V and h-V species of SM and Cer display between the head and the tail of mature spermatozoa.
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Mateos, Melina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Córdoba
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
MEMBRANE FRACTIONS
SPHINGOMYELINS
CERAMIDES
DIFFERENTIATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/237627
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/237627
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Low-density membrane fractions from male germ cells lack sphingolipids with very long chain PUFASantiago Valtierra, Florencia XimenaMateos, Melina ValeriaAveldaño, Marta IsabelOresti, Gerardo MartinMEMBRANE FRACTIONSSPHINGOMYELINSCERAMIDESDIFFERENTIATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sphingomyelins (SM) and ceramides (Cer) with very long-chain PUFA (VLCPUFA), in nonhydroxy (n-V) and 2-hydroxy (h-V) forms, are specific components of rat spermatogenic cells. Here we evaluated how differentiation affects their distribution among membrane fractions from such cells. Using a detergent-free procedure, a small light, raft-like low-density (L) fraction and a large heavier (H) fraction, both showing markers typical of cell plasma membranes, were separated from pachytene spermatocytes, round, and late spermatids. MALDI-TOF spectra showed that the L fraction had mostly SM species with saturated fatty acids regardless of the cell stage, while the H fraction was rich in stage-varying SM and Cer species with VLCPUFA. In this fraction spermatocytes accumulated mostly n-V SM and spermatids h-V SM and h-V Cer species. A third fraction made of intracellular membranes had less SM and more Cer than the H fraction, differentiation also increasing the h-V/n-V ratio in both lipids. The buildup of 2-hydroxy fatty acids correlated with the expression (mRNA) of fatty acid 2-hydroxylase (Fa2h), higher in spermatids than in spermatocytes. The differentiation-dependent rise in h-V Cer in the germ cell H fraction during spermatogenesis is consistent with the eventually uneven distribution that n-V and h-V species of SM and Cer display between the head and the tail of mature spermatozoa.Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Mateos, Melina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaLII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología MolecularCórdobaArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/237627Low-density membrane fractions from male germ cells lack sphingolipids with very long chain PUFA; LII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Córdoba; Argentina; 2016; 37-380327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v40nSuppl.S/34023/pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:27Zoai:ri.conicet.gov.ar:11336/237627instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:27.884CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Low-density membrane fractions from male germ cells lack sphingolipids with very long chain PUFA
title Low-density membrane fractions from male germ cells lack sphingolipids with very long chain PUFA
spellingShingle Low-density membrane fractions from male germ cells lack sphingolipids with very long chain PUFA
Santiago Valtierra, Florencia Ximena
MEMBRANE FRACTIONS
SPHINGOMYELINS
CERAMIDES
DIFFERENTIATION
title_short Low-density membrane fractions from male germ cells lack sphingolipids with very long chain PUFA
title_full Low-density membrane fractions from male germ cells lack sphingolipids with very long chain PUFA
title_fullStr Low-density membrane fractions from male germ cells lack sphingolipids with very long chain PUFA
title_full_unstemmed Low-density membrane fractions from male germ cells lack sphingolipids with very long chain PUFA
title_sort Low-density membrane fractions from male germ cells lack sphingolipids with very long chain PUFA
dc.creator.none.fl_str_mv Santiago Valtierra, Florencia Ximena
Mateos, Melina Valeria
Aveldaño, Marta Isabel
Oresti, Gerardo Martin
author Santiago Valtierra, Florencia Ximena
author_facet Santiago Valtierra, Florencia Ximena
Mateos, Melina Valeria
Aveldaño, Marta Isabel
Oresti, Gerardo Martin
author_role author
author2 Mateos, Melina Valeria
Aveldaño, Marta Isabel
Oresti, Gerardo Martin
author2_role author
author
author
dc.subject.none.fl_str_mv MEMBRANE FRACTIONS
SPHINGOMYELINS
CERAMIDES
DIFFERENTIATION
topic MEMBRANE FRACTIONS
SPHINGOMYELINS
CERAMIDES
DIFFERENTIATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sphingomyelins (SM) and ceramides (Cer) with very long-chain PUFA (VLCPUFA), in nonhydroxy (n-V) and 2-hydroxy (h-V) forms, are specific components of rat spermatogenic cells. Here we evaluated how differentiation affects their distribution among membrane fractions from such cells. Using a detergent-free procedure, a small light, raft-like low-density (L) fraction and a large heavier (H) fraction, both showing markers typical of cell plasma membranes, were separated from pachytene spermatocytes, round, and late spermatids. MALDI-TOF spectra showed that the L fraction had mostly SM species with saturated fatty acids regardless of the cell stage, while the H fraction was rich in stage-varying SM and Cer species with VLCPUFA. In this fraction spermatocytes accumulated mostly n-V SM and spermatids h-V SM and h-V Cer species. A third fraction made of intracellular membranes had less SM and more Cer than the H fraction, differentiation also increasing the h-V/n-V ratio in both lipids. The buildup of 2-hydroxy fatty acids correlated with the expression (mRNA) of fatty acid 2-hydroxylase (Fa2h), higher in spermatids than in spermatocytes. The differentiation-dependent rise in h-V Cer in the germ cell H fraction during spermatogenesis is consistent with the eventually uneven distribution that n-V and h-V species of SM and Cer display between the head and the tail of mature spermatozoa.
Fil: Santiago Valtierra, Florencia Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Mateos, Melina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Córdoba
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Sphingomyelins (SM) and ceramides (Cer) with very long-chain PUFA (VLCPUFA), in nonhydroxy (n-V) and 2-hydroxy (h-V) forms, are specific components of rat spermatogenic cells. Here we evaluated how differentiation affects their distribution among membrane fractions from such cells. Using a detergent-free procedure, a small light, raft-like low-density (L) fraction and a large heavier (H) fraction, both showing markers typical of cell plasma membranes, were separated from pachytene spermatocytes, round, and late spermatids. MALDI-TOF spectra showed that the L fraction had mostly SM species with saturated fatty acids regardless of the cell stage, while the H fraction was rich in stage-varying SM and Cer species with VLCPUFA. In this fraction spermatocytes accumulated mostly n-V SM and spermatids h-V SM and h-V Cer species. A third fraction made of intracellular membranes had less SM and more Cer than the H fraction, differentiation also increasing the h-V/n-V ratio in both lipids. The buildup of 2-hydroxy fatty acids correlated with the expression (mRNA) of fatty acid 2-hydroxylase (Fa2h), higher in spermatids than in spermatocytes. The differentiation-dependent rise in h-V Cer in the germ cell H fraction during spermatogenesis is consistent with the eventually uneven distribution that n-V and h-V species of SM and Cer display between the head and the tail of mature spermatozoa.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/237627
Low-density membrane fractions from male germ cells lack sphingolipids with very long chain PUFA; LII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Córdoba; Argentina; 2016; 37-38
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/237627
identifier_str_mv Low-density membrane fractions from male germ cells lack sphingolipids with very long chain PUFA; LII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Córdoba; Argentina; 2016; 37-38
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v40nSuppl.S/34023/pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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