Eubacterial populations in the apple snail’s digestive gland

Autores
Dell Agnola, Federico Agustín; Sánchez Puerta, María Virginia; Castro Vazquez, Alfredo Juan; Vega, Israel Aníbal
Año de publicación
2024
Idioma
inglés
Tipo de recurso
conjunto de datos
Estado
Descripción
BACKGROUND: the invasive gastropod Pomacea canaliculata hosts bacterial endosymbionts in the gut. Here, we described the taxonomy and phylogeny of the bacterial biodiversity residing in the digestive gland (DG) and then highlight the metabolic characteristics of these microorganisms and their potential involvement in the host´s digestive physiology. RESULTS: a library of symbiotic 16S rDNA genes was built and analysed in silico. The sequences were classified into seven bacterial phyla: Bacillota, Pseudomonadota, Thermodesulfobacteriota, Bacteroidota, Chlorobiota, Chloroflexota, and Verrucomicrobiota. It was defined 26 Operational Taxonomic Units at 97% of genetic distance. Rarefaction curves and ecological indices showed that the DG bacterial community was diverse but non-evenness, with Lachnospiraceae and Azonexaceae as dominant OTUs. Most eubacterial OTUs were strict or facultative anaerobes, capable of digesting dietary β-polysaccharides, such as cellulose and chitin, and proteins (65%). Moreover, some of these bacteria would reduce metals (over 50%) and fix nitrogen (37%). Other OTUs were lactic acid-producing bacteria (40%) and/or short-chain fatty acids-producing bacteria (SCFAs Clostridium-like; 35%), sulphate-reducing bacteria (38%) and perchlorate-reducing bacteria (17%). CONCLUSION: this bacterial community may be beneficial for the host as it potentially would enhance macronutrient extracellular digestion, inorganic nitrogen assimilation, and aquatic xenobiotics detoxification.
Fil: Dell Agnola, Federico Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina
Fil: Sánchez Puerta, María Virginia. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina
Fil: Castro Vazquez, Alfredo Juan. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina
Fil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/248214
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Eubacterial populations in the apple snail’s digestive glandDell Agnola, Federico AgustínSánchez Puerta, María VirginiaCastro Vazquez, Alfredo JuanVega, Israel Aníbalhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1BACKGROUND: the invasive gastropod Pomacea canaliculata hosts bacterial endosymbionts in the gut. Here, we described the taxonomy and phylogeny of the bacterial biodiversity residing in the digestive gland (DG) and then highlight the metabolic characteristics of these microorganisms and their potential involvement in the host´s digestive physiology. RESULTS: a library of symbiotic 16S rDNA genes was built and analysed in silico. The sequences were classified into seven bacterial phyla: Bacillota, Pseudomonadota, Thermodesulfobacteriota, Bacteroidota, Chlorobiota, Chloroflexota, and Verrucomicrobiota. It was defined 26 Operational Taxonomic Units at 97% of genetic distance. Rarefaction curves and ecological indices showed that the DG bacterial community was diverse but non-evenness, with Lachnospiraceae and Azonexaceae as dominant OTUs. Most eubacterial OTUs were strict or facultative anaerobes, capable of digesting dietary β-polysaccharides, such as cellulose and chitin, and proteins (65%). Moreover, some of these bacteria would reduce metals (over 50%) and fix nitrogen (37%). Other OTUs were lactic acid-producing bacteria (40%) and/or short-chain fatty acids-producing bacteria (SCFAs Clostridium-like; 35%), sulphate-reducing bacteria (38%) and perchlorate-reducing bacteria (17%). CONCLUSION: this bacterial community may be beneficial for the host as it potentially would enhance macronutrient extracellular digestion, inorganic nitrogen assimilation, and aquatic xenobiotics detoxification.Fil: Dell Agnola, Federico Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; ArgentinaFil: Sánchez Puerta, María Virginia. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; ArgentinaFil: Castro Vazquez, Alfredo Juan. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; ArgentinaFil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina2024info:ar-repo/semantics/conjuntoDeDatosv1.0info:eu-repo/semantics/dataSetapplication/ziphttp://hdl.handle.net/11336/248214Dell Agnola, Federico Agustín; Sánchez Puerta, María Virginia; Castro Vazquez, Alfredo Juan; Vega, Israel Aníbal; (2024): Eubacterial populations in the apple snail’s digestive gland. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/248214CONICET DigitalCONICETenginfo:eu-repo/grantAgreement/Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica/ANPCyT-FoNCyT PICT-2020-SERIEA-03638info:eu-repo/grantAgreement/Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica/ANPCyT-FoNCyT PICT-2020-SERIEA-03638info:eu-repo/grantAgreement/Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología/ANPCyT-FoNCyT PICT-2020-SERIEA-03638info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:07:57Zoai:ri.conicet.gov.ar:11336/248214instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:07:57.794CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Eubacterial populations in the apple snail’s digestive gland
title Eubacterial populations in the apple snail’s digestive gland
spellingShingle Eubacterial populations in the apple snail’s digestive gland
Dell Agnola, Federico Agustín
title_short Eubacterial populations in the apple snail’s digestive gland
title_full Eubacterial populations in the apple snail’s digestive gland
title_fullStr Eubacterial populations in the apple snail’s digestive gland
title_full_unstemmed Eubacterial populations in the apple snail’s digestive gland
title_sort Eubacterial populations in the apple snail’s digestive gland
dc.creator.none.fl_str_mv Dell Agnola, Federico Agustín
Sánchez Puerta, María Virginia
Castro Vazquez, Alfredo Juan
Vega, Israel Aníbal
author Dell Agnola, Federico Agustín
author_facet Dell Agnola, Federico Agustín
Sánchez Puerta, María Virginia
Castro Vazquez, Alfredo Juan
Vega, Israel Aníbal
author_role author
author2 Sánchez Puerta, María Virginia
Castro Vazquez, Alfredo Juan
Vega, Israel Aníbal
author2_role author
author
author
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv BACKGROUND: the invasive gastropod Pomacea canaliculata hosts bacterial endosymbionts in the gut. Here, we described the taxonomy and phylogeny of the bacterial biodiversity residing in the digestive gland (DG) and then highlight the metabolic characteristics of these microorganisms and their potential involvement in the host´s digestive physiology. RESULTS: a library of symbiotic 16S rDNA genes was built and analysed in silico. The sequences were classified into seven bacterial phyla: Bacillota, Pseudomonadota, Thermodesulfobacteriota, Bacteroidota, Chlorobiota, Chloroflexota, and Verrucomicrobiota. It was defined 26 Operational Taxonomic Units at 97% of genetic distance. Rarefaction curves and ecological indices showed that the DG bacterial community was diverse but non-evenness, with Lachnospiraceae and Azonexaceae as dominant OTUs. Most eubacterial OTUs were strict or facultative anaerobes, capable of digesting dietary β-polysaccharides, such as cellulose and chitin, and proteins (65%). Moreover, some of these bacteria would reduce metals (over 50%) and fix nitrogen (37%). Other OTUs were lactic acid-producing bacteria (40%) and/or short-chain fatty acids-producing bacteria (SCFAs Clostridium-like; 35%), sulphate-reducing bacteria (38%) and perchlorate-reducing bacteria (17%). CONCLUSION: this bacterial community may be beneficial for the host as it potentially would enhance macronutrient extracellular digestion, inorganic nitrogen assimilation, and aquatic xenobiotics detoxification.
Fil: Dell Agnola, Federico Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina
Fil: Sánchez Puerta, María Virginia. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina
Fil: Castro Vazquez, Alfredo Juan. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina
Fil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
description BACKGROUND: the invasive gastropod Pomacea canaliculata hosts bacterial endosymbionts in the gut. Here, we described the taxonomy and phylogeny of the bacterial biodiversity residing in the digestive gland (DG) and then highlight the metabolic characteristics of these microorganisms and their potential involvement in the host´s digestive physiology. RESULTS: a library of symbiotic 16S rDNA genes was built and analysed in silico. The sequences were classified into seven bacterial phyla: Bacillota, Pseudomonadota, Thermodesulfobacteriota, Bacteroidota, Chlorobiota, Chloroflexota, and Verrucomicrobiota. It was defined 26 Operational Taxonomic Units at 97% of genetic distance. Rarefaction curves and ecological indices showed that the DG bacterial community was diverse but non-evenness, with Lachnospiraceae and Azonexaceae as dominant OTUs. Most eubacterial OTUs were strict or facultative anaerobes, capable of digesting dietary β-polysaccharides, such as cellulose and chitin, and proteins (65%). Moreover, some of these bacteria would reduce metals (over 50%) and fix nitrogen (37%). Other OTUs were lactic acid-producing bacteria (40%) and/or short-chain fatty acids-producing bacteria (SCFAs Clostridium-like; 35%), sulphate-reducing bacteria (38%) and perchlorate-reducing bacteria (17%). CONCLUSION: this bacterial community may be beneficial for the host as it potentially would enhance macronutrient extracellular digestion, inorganic nitrogen assimilation, and aquatic xenobiotics detoxification.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:ar-repo/semantics/conjuntoDeDatos
v1.0
info:eu-repo/semantics/dataSet
format dataSet
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/248214
Dell Agnola, Federico Agustín; Sánchez Puerta, María Virginia; Castro Vazquez, Alfredo Juan; Vega, Israel Aníbal; (2024): Eubacterial populations in the apple snail’s digestive gland. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/248214
CONICET Digital
CONICET
url http://hdl.handle.net/11336/248214
identifier_str_mv Dell Agnola, Federico Agustín; Sánchez Puerta, María Virginia; Castro Vazquez, Alfredo Juan; Vega, Israel Aníbal; (2024): Eubacterial populations in the apple snail’s digestive gland. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/248214
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica/ANPCyT-FoNCyT PICT-2020-SERIEA-03638
info:eu-repo/grantAgreement/Ministerio de Ciencia, Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica. Fondo para la Investigación Científica y Tecnológica/ANPCyT-FoNCyT PICT-2020-SERIEA-03638
info:eu-repo/grantAgreement/Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología/ANPCyT-FoNCyT PICT-2020-SERIEA-03638
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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