Evidence for a prokaryotic origin of intracellular corpuscles in the digestive gland of the queen conch Lobatus gigas (Linnaeus, 1758) (Gastropoda: Strombidae)

Autores
Dell Agnola, Federico Agustín; Vega, Israel Aníbal; Castro Vazquez, Alfredo Juan
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Two types of pigmented intracellular bodies have been reported in the digestive glands of several caenogastropods, particularly in the families Ampullariidae (Architaenioglossa: Ampullarioidea), Strombidae and Struthiolariidae (Littorinimorpha: Stromboidea). Rounded corpuscles, which are usually light brown, are identified here as C corpuscles, while corpuscles that are oval, darker and larger are identified as K corpuscles. We studied both corpuscular types in Lobatus gigas (Strombidae) using (1) differential-interference contrast microscopy, (2) transmission electron microscopy, (3) in situ hybridization with a generalized cyanobacterial 16S rRNA probe and (4) autofluorescence before and after lysozyme digestion. Results indicated that C corpuscles were located in the basal regions of columnar cells and the intensity of their pigmentation and alcianophily (indicative of glycosaminoglycans) was variable. They showed an electrondense wall and contained abundant electron-dense clumps and irregularly arranged membranes, but no thylakoids or nuclei. Hybridization with the 16S rRNA probe varied from none to intense in C corpuscles, indicative of variations in the rRNA content during their life cycles. Their walls were sensitive to lysozyme digestion, which strongly suggests that peptidoglycans are an integral part of this structure. K corpuscles were located within pyramidal cells and were uniformly dark brown but variably alcianophilic. They showed multiple lamellae of moderate electron density, organized around one to three cores, each one containing one or several small spherical bodies. All K corpuscles hybridized with the 16S rRNA probe and were partly digested by lysozyme. Both C and K corpuscles showed red autofluorescence, which suggests the presence of chlorophyll-like pigments. It is concluded that C and K corpuscles in the digestive gland of L. gigas may be forms of a prokaryotic symbiont related to the Cyanobacteria.
Fil: Dell Agnola, Federico Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Castro Vazquez, Alfredo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Materia
Endosymbiosis
Digestive Gland
Littorinimorpha
Strombidae
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/49392
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Evidence for a prokaryotic origin of intracellular corpuscles in the digestive gland of the queen conch Lobatus gigas (Linnaeus, 1758) (Gastropoda: Strombidae)Dell Agnola, Federico AgustínVega, Israel AníbalCastro Vazquez, Alfredo JuanEndosymbiosisDigestive GlandLittorinimorphaStrombidaehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Two types of pigmented intracellular bodies have been reported in the digestive glands of several caenogastropods, particularly in the families Ampullariidae (Architaenioglossa: Ampullarioidea), Strombidae and Struthiolariidae (Littorinimorpha: Stromboidea). Rounded corpuscles, which are usually light brown, are identified here as C corpuscles, while corpuscles that are oval, darker and larger are identified as K corpuscles. We studied both corpuscular types in Lobatus gigas (Strombidae) using (1) differential-interference contrast microscopy, (2) transmission electron microscopy, (3) in situ hybridization with a generalized cyanobacterial 16S rRNA probe and (4) autofluorescence before and after lysozyme digestion. Results indicated that C corpuscles were located in the basal regions of columnar cells and the intensity of their pigmentation and alcianophily (indicative of glycosaminoglycans) was variable. They showed an electrondense wall and contained abundant electron-dense clumps and irregularly arranged membranes, but no thylakoids or nuclei. Hybridization with the 16S rRNA probe varied from none to intense in C corpuscles, indicative of variations in the rRNA content during their life cycles. Their walls were sensitive to lysozyme digestion, which strongly suggests that peptidoglycans are an integral part of this structure. K corpuscles were located within pyramidal cells and were uniformly dark brown but variably alcianophilic. They showed multiple lamellae of moderate electron density, organized around one to three cores, each one containing one or several small spherical bodies. All K corpuscles hybridized with the 16S rRNA probe and were partly digested by lysozyme. Both C and K corpuscles showed red autofluorescence, which suggests the presence of chlorophyll-like pigments. It is concluded that C and K corpuscles in the digestive gland of L. gigas may be forms of a prokaryotic symbiont related to the Cyanobacteria.Fil: Dell Agnola, Federico Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Castro Vazquez, Alfredo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaOxford University Press2017-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49392Dell Agnola, Federico Agustín; Vega, Israel Aníbal; Castro Vazquez, Alfredo Juan; Evidence for a prokaryotic origin of intracellular corpuscles in the digestive gland of the queen conch Lobatus gigas (Linnaeus, 1758) (Gastropoda: Strombidae); Oxford University Press; Journal of Molluscan Studies; 83; 2; 5-2017; 186-1930260-1230CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/mollus/article/83/2/186/3053046info:eu-repo/semantics/altIdentifier/doi/10.1093/mollus/eyx003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:38:32Zoai:ri.conicet.gov.ar:11336/49392instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:38:32.732CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evidence for a prokaryotic origin of intracellular corpuscles in the digestive gland of the queen conch Lobatus gigas (Linnaeus, 1758) (Gastropoda: Strombidae)
title Evidence for a prokaryotic origin of intracellular corpuscles in the digestive gland of the queen conch Lobatus gigas (Linnaeus, 1758) (Gastropoda: Strombidae)
spellingShingle Evidence for a prokaryotic origin of intracellular corpuscles in the digestive gland of the queen conch Lobatus gigas (Linnaeus, 1758) (Gastropoda: Strombidae)
Dell Agnola, Federico Agustín
Endosymbiosis
Digestive Gland
Littorinimorpha
Strombidae
title_short Evidence for a prokaryotic origin of intracellular corpuscles in the digestive gland of the queen conch Lobatus gigas (Linnaeus, 1758) (Gastropoda: Strombidae)
title_full Evidence for a prokaryotic origin of intracellular corpuscles in the digestive gland of the queen conch Lobatus gigas (Linnaeus, 1758) (Gastropoda: Strombidae)
title_fullStr Evidence for a prokaryotic origin of intracellular corpuscles in the digestive gland of the queen conch Lobatus gigas (Linnaeus, 1758) (Gastropoda: Strombidae)
title_full_unstemmed Evidence for a prokaryotic origin of intracellular corpuscles in the digestive gland of the queen conch Lobatus gigas (Linnaeus, 1758) (Gastropoda: Strombidae)
title_sort Evidence for a prokaryotic origin of intracellular corpuscles in the digestive gland of the queen conch Lobatus gigas (Linnaeus, 1758) (Gastropoda: Strombidae)
dc.creator.none.fl_str_mv Dell Agnola, Federico Agustín
Vega, Israel Aníbal
Castro Vazquez, Alfredo Juan
author Dell Agnola, Federico Agustín
author_facet Dell Agnola, Federico Agustín
Vega, Israel Aníbal
Castro Vazquez, Alfredo Juan
author_role author
author2 Vega, Israel Aníbal
Castro Vazquez, Alfredo Juan
author2_role author
author
dc.subject.none.fl_str_mv Endosymbiosis
Digestive Gland
Littorinimorpha
Strombidae
topic Endosymbiosis
Digestive Gland
Littorinimorpha
Strombidae
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Two types of pigmented intracellular bodies have been reported in the digestive glands of several caenogastropods, particularly in the families Ampullariidae (Architaenioglossa: Ampullarioidea), Strombidae and Struthiolariidae (Littorinimorpha: Stromboidea). Rounded corpuscles, which are usually light brown, are identified here as C corpuscles, while corpuscles that are oval, darker and larger are identified as K corpuscles. We studied both corpuscular types in Lobatus gigas (Strombidae) using (1) differential-interference contrast microscopy, (2) transmission electron microscopy, (3) in situ hybridization with a generalized cyanobacterial 16S rRNA probe and (4) autofluorescence before and after lysozyme digestion. Results indicated that C corpuscles were located in the basal regions of columnar cells and the intensity of their pigmentation and alcianophily (indicative of glycosaminoglycans) was variable. They showed an electrondense wall and contained abundant electron-dense clumps and irregularly arranged membranes, but no thylakoids or nuclei. Hybridization with the 16S rRNA probe varied from none to intense in C corpuscles, indicative of variations in the rRNA content during their life cycles. Their walls were sensitive to lysozyme digestion, which strongly suggests that peptidoglycans are an integral part of this structure. K corpuscles were located within pyramidal cells and were uniformly dark brown but variably alcianophilic. They showed multiple lamellae of moderate electron density, organized around one to three cores, each one containing one or several small spherical bodies. All K corpuscles hybridized with the 16S rRNA probe and were partly digested by lysozyme. Both C and K corpuscles showed red autofluorescence, which suggests the presence of chlorophyll-like pigments. It is concluded that C and K corpuscles in the digestive gland of L. gigas may be forms of a prokaryotic symbiont related to the Cyanobacteria.
Fil: Dell Agnola, Federico Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Castro Vazquez, Alfredo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
description Two types of pigmented intracellular bodies have been reported in the digestive glands of several caenogastropods, particularly in the families Ampullariidae (Architaenioglossa: Ampullarioidea), Strombidae and Struthiolariidae (Littorinimorpha: Stromboidea). Rounded corpuscles, which are usually light brown, are identified here as C corpuscles, while corpuscles that are oval, darker and larger are identified as K corpuscles. We studied both corpuscular types in Lobatus gigas (Strombidae) using (1) differential-interference contrast microscopy, (2) transmission electron microscopy, (3) in situ hybridization with a generalized cyanobacterial 16S rRNA probe and (4) autofluorescence before and after lysozyme digestion. Results indicated that C corpuscles were located in the basal regions of columnar cells and the intensity of their pigmentation and alcianophily (indicative of glycosaminoglycans) was variable. They showed an electrondense wall and contained abundant electron-dense clumps and irregularly arranged membranes, but no thylakoids or nuclei. Hybridization with the 16S rRNA probe varied from none to intense in C corpuscles, indicative of variations in the rRNA content during their life cycles. Their walls were sensitive to lysozyme digestion, which strongly suggests that peptidoglycans are an integral part of this structure. K corpuscles were located within pyramidal cells and were uniformly dark brown but variably alcianophilic. They showed multiple lamellae of moderate electron density, organized around one to three cores, each one containing one or several small spherical bodies. All K corpuscles hybridized with the 16S rRNA probe and were partly digested by lysozyme. Both C and K corpuscles showed red autofluorescence, which suggests the presence of chlorophyll-like pigments. It is concluded that C and K corpuscles in the digestive gland of L. gigas may be forms of a prokaryotic symbiont related to the Cyanobacteria.
publishDate 2017
dc.date.none.fl_str_mv 2017-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49392
Dell Agnola, Federico Agustín; Vega, Israel Aníbal; Castro Vazquez, Alfredo Juan; Evidence for a prokaryotic origin of intracellular corpuscles in the digestive gland of the queen conch Lobatus gigas (Linnaeus, 1758) (Gastropoda: Strombidae); Oxford University Press; Journal of Molluscan Studies; 83; 2; 5-2017; 186-193
0260-1230
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49392
identifier_str_mv Dell Agnola, Federico Agustín; Vega, Israel Aníbal; Castro Vazquez, Alfredo Juan; Evidence for a prokaryotic origin of intracellular corpuscles in the digestive gland of the queen conch Lobatus gigas (Linnaeus, 1758) (Gastropoda: Strombidae); Oxford University Press; Journal of Molluscan Studies; 83; 2; 5-2017; 186-193
0260-1230
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/mollus/article/83/2/186/3053046
info:eu-repo/semantics/altIdentifier/doi/10.1093/mollus/eyx003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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