Apple snails as vectors of trematodes: molecular identification of the larval stages that parasites the digestive gland of some argentinian ampullariids

Autores
Dellagnola, F. A.; Gentile, L.; Vega, Israel Aníbal
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In the context of a broader program dealing with the symbiotic associations of apple snails, wesampled some species of Neotropical ampullariids that occurs mainly in the Paraná-Uruguayriver basins (Argentina). Different larval stages (sporocysts, rediae, and cercariae) of flukes arefound in the digestive gland of these freshwater snails. Asolene platae showed trematode larvae(echinocercariae and xiphidiocercariae) in haemocoelic spaces and connective tissue of thedigestive gland. The echinocercaria resembled those of the genus Echinochasmus, but lackedsensory hairs on the body and tail whereas the xiphidiocercaria was similar to theXiphidiocercaria armatae belonging to the Opisthoglyphe type. Also, we described for the firsttime the cercarial stage of Stomylotrema vicarium in the host Pomacea americanista fromMisiones rain forest, which showed a big stylet in the oral sucker and three pairs of penetrationglands. Moreover, a molecular multilocus approach (using the 28S rRNA, ITS1 and mtCOXIsequences) was used to infer the phylogenetic position and understand their life cycle. The 28SrRNA gene linked the echinocercarial sequences with the polyphyletic genus Echinochasmus,while the xiphidiocercarial sequences were linked with the genus Phaneropsolus. Sequences ofthe trematode larval stages from P. americanista were grouped (genetic distance ranged 0.02%and 0.05%) with two sequences from adult Stomylotrema vicarium. Both ITS1 y mtCOXIsequences confirmed the phylogenetic position of the larvae inside of Echinochasmidae,Phaneropsolidae, and Stomylotrematidae. Finally, the three molecular markers were useful todistinguish cryptic molecular entities from a single echinocercarial morphotype, morphologicallyundistinguished, in the host A. platae.
Fil: Dellagnola, F. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina
Fil: Gentile, L.. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
XI Congreso Latinoamericano de Malacología
Perú
Asociación Argentina de Malacología
Materia
AMPULLARIIDAE
TREMATODA
RRNA 28S
ITS1
MTCOXI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/248892
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/248892
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Apple snails as vectors of trematodes: molecular identification of the larval stages that parasites the digestive gland of some argentinian ampullariidsDellagnola, F. A.Gentile, L.Vega, Israel AníbalAMPULLARIIDAETREMATODARRNA 28SITS1MTCOXIhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the context of a broader program dealing with the symbiotic associations of apple snails, wesampled some species of Neotropical ampullariids that occurs mainly in the Paraná-Uruguayriver basins (Argentina). Different larval stages (sporocysts, rediae, and cercariae) of flukes arefound in the digestive gland of these freshwater snails. Asolene platae showed trematode larvae(echinocercariae and xiphidiocercariae) in haemocoelic spaces and connective tissue of thedigestive gland. The echinocercaria resembled those of the genus Echinochasmus, but lackedsensory hairs on the body and tail whereas the xiphidiocercaria was similar to theXiphidiocercaria armatae belonging to the Opisthoglyphe type. Also, we described for the firsttime the cercarial stage of Stomylotrema vicarium in the host Pomacea americanista fromMisiones rain forest, which showed a big stylet in the oral sucker and three pairs of penetrationglands. Moreover, a molecular multilocus approach (using the 28S rRNA, ITS1 and mtCOXIsequences) was used to infer the phylogenetic position and understand their life cycle. The 28SrRNA gene linked the echinocercarial sequences with the polyphyletic genus Echinochasmus,while the xiphidiocercarial sequences were linked with the genus Phaneropsolus. Sequences ofthe trematode larval stages from P. americanista were grouped (genetic distance ranged 0.02%and 0.05%) with two sequences from adult Stomylotrema vicarium. Both ITS1 y mtCOXIsequences confirmed the phylogenetic position of the larvae inside of Echinochasmidae,Phaneropsolidae, and Stomylotrematidae. Finally, the three molecular markers were useful todistinguish cryptic molecular entities from a single echinocercarial morphotype, morphologicallyundistinguished, in the host A. platae.Fil: Dellagnola, F. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; ArgentinaFil: Gentile, L.. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; ArgentinaFil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaXI Congreso Latinoamericano de MalacologíaPerúAsociación Argentina de MalacologíaAsociación Argentina de Malacología2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/248892Apple snails as vectors of trematodes: molecular identification of the larval stages that parasites the digestive gland of some argentinian ampullariids; XI Congreso Latinoamericano de Malacología; Perú; 2020; 1-1CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://malacoargentina.com.ar/blog/wp-content/uploads/2021/06/Libro-de-Resumenes-XI-CLAMA-2020-2.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:31Zoai:ri.conicet.gov.ar:11336/248892instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:31.729CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Apple snails as vectors of trematodes: molecular identification of the larval stages that parasites the digestive gland of some argentinian ampullariids
title Apple snails as vectors of trematodes: molecular identification of the larval stages that parasites the digestive gland of some argentinian ampullariids
spellingShingle Apple snails as vectors of trematodes: molecular identification of the larval stages that parasites the digestive gland of some argentinian ampullariids
Dellagnola, F. A.
AMPULLARIIDAE
TREMATODA
RRNA 28S
ITS1
MTCOXI
title_short Apple snails as vectors of trematodes: molecular identification of the larval stages that parasites the digestive gland of some argentinian ampullariids
title_full Apple snails as vectors of trematodes: molecular identification of the larval stages that parasites the digestive gland of some argentinian ampullariids
title_fullStr Apple snails as vectors of trematodes: molecular identification of the larval stages that parasites the digestive gland of some argentinian ampullariids
title_full_unstemmed Apple snails as vectors of trematodes: molecular identification of the larval stages that parasites the digestive gland of some argentinian ampullariids
title_sort Apple snails as vectors of trematodes: molecular identification of the larval stages that parasites the digestive gland of some argentinian ampullariids
dc.creator.none.fl_str_mv Dellagnola, F. A.
Gentile, L.
Vega, Israel Aníbal
author Dellagnola, F. A.
author_facet Dellagnola, F. A.
Gentile, L.
Vega, Israel Aníbal
author_role author
author2 Gentile, L.
Vega, Israel Aníbal
author2_role author
author
dc.subject.none.fl_str_mv AMPULLARIIDAE
TREMATODA
RRNA 28S
ITS1
MTCOXI
topic AMPULLARIIDAE
TREMATODA
RRNA 28S
ITS1
MTCOXI
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the context of a broader program dealing with the symbiotic associations of apple snails, wesampled some species of Neotropical ampullariids that occurs mainly in the Paraná-Uruguayriver basins (Argentina). Different larval stages (sporocysts, rediae, and cercariae) of flukes arefound in the digestive gland of these freshwater snails. Asolene platae showed trematode larvae(echinocercariae and xiphidiocercariae) in haemocoelic spaces and connective tissue of thedigestive gland. The echinocercaria resembled those of the genus Echinochasmus, but lackedsensory hairs on the body and tail whereas the xiphidiocercaria was similar to theXiphidiocercaria armatae belonging to the Opisthoglyphe type. Also, we described for the firsttime the cercarial stage of Stomylotrema vicarium in the host Pomacea americanista fromMisiones rain forest, which showed a big stylet in the oral sucker and three pairs of penetrationglands. Moreover, a molecular multilocus approach (using the 28S rRNA, ITS1 and mtCOXIsequences) was used to infer the phylogenetic position and understand their life cycle. The 28SrRNA gene linked the echinocercarial sequences with the polyphyletic genus Echinochasmus,while the xiphidiocercarial sequences were linked with the genus Phaneropsolus. Sequences ofthe trematode larval stages from P. americanista were grouped (genetic distance ranged 0.02%and 0.05%) with two sequences from adult Stomylotrema vicarium. Both ITS1 y mtCOXIsequences confirmed the phylogenetic position of the larvae inside of Echinochasmidae,Phaneropsolidae, and Stomylotrematidae. Finally, the three molecular markers were useful todistinguish cryptic molecular entities from a single echinocercarial morphotype, morphologicallyundistinguished, in the host A. platae.
Fil: Dellagnola, F. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina
Fil: Gentile, L.. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
XI Congreso Latinoamericano de Malacología
Perú
Asociación Argentina de Malacología
description In the context of a broader program dealing with the symbiotic associations of apple snails, wesampled some species of Neotropical ampullariids that occurs mainly in the Paraná-Uruguayriver basins (Argentina). Different larval stages (sporocysts, rediae, and cercariae) of flukes arefound in the digestive gland of these freshwater snails. Asolene platae showed trematode larvae(echinocercariae and xiphidiocercariae) in haemocoelic spaces and connective tissue of thedigestive gland. The echinocercaria resembled those of the genus Echinochasmus, but lackedsensory hairs on the body and tail whereas the xiphidiocercaria was similar to theXiphidiocercaria armatae belonging to the Opisthoglyphe type. Also, we described for the firsttime the cercarial stage of Stomylotrema vicarium in the host Pomacea americanista fromMisiones rain forest, which showed a big stylet in the oral sucker and three pairs of penetrationglands. Moreover, a molecular multilocus approach (using the 28S rRNA, ITS1 and mtCOXIsequences) was used to infer the phylogenetic position and understand their life cycle. The 28SrRNA gene linked the echinocercarial sequences with the polyphyletic genus Echinochasmus,while the xiphidiocercarial sequences were linked with the genus Phaneropsolus. Sequences ofthe trematode larval stages from P. americanista were grouped (genetic distance ranged 0.02%and 0.05%) with two sequences from adult Stomylotrema vicarium. Both ITS1 y mtCOXIsequences confirmed the phylogenetic position of the larvae inside of Echinochasmidae,Phaneropsolidae, and Stomylotrematidae. Finally, the three molecular markers were useful todistinguish cryptic molecular entities from a single echinocercarial morphotype, morphologicallyundistinguished, in the host A. platae.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/248892
Apple snails as vectors of trematodes: molecular identification of the larval stages that parasites the digestive gland of some argentinian ampullariids; XI Congreso Latinoamericano de Malacología; Perú; 2020; 1-1
CONICET Digital
CONICET
url http://hdl.handle.net/11336/248892
identifier_str_mv Apple snails as vectors of trematodes: molecular identification of the larval stages that parasites the digestive gland of some argentinian ampullariids; XI Congreso Latinoamericano de Malacología; Perú; 2020; 1-1
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://malacoargentina.com.ar/blog/wp-content/uploads/2021/06/Libro-de-Resumenes-XI-CLAMA-2020-2.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Asociación Argentina de Malacología
publisher.none.fl_str_mv Asociación Argentina de Malacología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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