A multiple comparative study of putative endosymbionts in three coexisting apple snail species

Autores
Dell Agnola, Federico Agustín; Rodríguez, Cristian; Castro Vazquez, Alfredo Juan; Vega, Israel Aníbal
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We here compare morphological and molecular characters of some putative endosymbioticelements of the digestive gland of three ampullariid species (Pomacea canaliculata,Pomacea scalaris and Asolene platae) which coexist in Lake Regatas (Palermo, BuenosAires). The putative endosymbionts were reported in these species and were identifiedas C and K corpuscles. The three species show tubuloacinar glands, each adenomerewas constituted mainly by two distinct cell types (columnar and pyramidal). C and Kcorpuscles together occupied from one-fourth to one-fifth of the tissue area in the threehost species, where C corpuscles were round and greenish-brown, were delimited bya distinct wall, stained positively with Alcian Blue and were associated with columnarcells. K corpuscles were oval, dark-brown multilamellar bodies and were associatedwith pyramidal cells. Under TEM, C corpuscles occurred within vacuoles of columnarcells and contained many electron-dense clumps and irregular membrane stacks andvesicles spread in an electron-lucent matrix. Sometimes a membrane appeared detachedfrom the inner surface of the wall, suggesting the existence of a plasma membrane. Inturn, K corpuscles were contained within vacuoles of pyramidal cells and were madeof concentric lamellae, which were in turn made of an electron-dense fibrogranularmaterial. No membranes were seen in them. Interspecifically, C corpuscles varysignificantly in width and inner contents. K corpuscles were also variable in lengthand width. However, both C and K corpuscles in the three studied species hybridisedwith generalised cyanobacterial/chloroplast probes for 16S rRNA. Also, both corpuscletypes (isolated from gland homogenates) were sensitive to lysozyme digestion, whichindicates that bacterial peptidoglycans are an integral part of their covers. The reporteddata confirm and extend previous studies on P. canaliculata in which the endosymbioticnature of C and K corpuscles were first proposed. We further propose that theendosymbiotic corpuscles are related to the Cyanobacteria/chloroplasts clade. Basedon the known distribution of these corpuscles in the major clades of Ampullariidae,we hypothesise they may be universally distributed in this family, and that mayconstitute an interesting model for studying the co-evolution of endosymbionts andtheir gastropod hosts.
Fil: Dell Agnola, Federico Agustín. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: Rodríguez, Cristian. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Castro Vazquez, Alfredo Juan. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina
Materia
AMPULLARIIDAE
POMACEA
ASOLENE
INTRACELLULAR BACTERIA
ENDOSYMBIOSIS
COEVOLUTION
DIGESTIVE GLAND
MORPHOMETRY
16S rRNA
FISH
LYSOZYME SENSITIVITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/109362
Acceder
id CONICETDig_df096804021b982453d8347625239eac
oai_identifier_str oai:ri.conicet.gov.ar:11336/109362
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A multiple comparative study of putative endosymbionts in three coexisting apple snail speciesDell Agnola, Federico AgustínRodríguez, CristianCastro Vazquez, Alfredo JuanVega, Israel AníbalAMPULLARIIDAEPOMACEAASOLENEINTRACELLULAR BACTERIAENDOSYMBIOSISCOEVOLUTIONDIGESTIVE GLANDMORPHOMETRY16S rRNAFISHLYSOZYME SENSITIVITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We here compare morphological and molecular characters of some putative endosymbioticelements of the digestive gland of three ampullariid species (Pomacea canaliculata,Pomacea scalaris and Asolene platae) which coexist in Lake Regatas (Palermo, BuenosAires). The putative endosymbionts were reported in these species and were identifiedas C and K corpuscles. The three species show tubuloacinar glands, each adenomerewas constituted mainly by two distinct cell types (columnar and pyramidal). C and Kcorpuscles together occupied from one-fourth to one-fifth of the tissue area in the threehost species, where C corpuscles were round and greenish-brown, were delimited bya distinct wall, stained positively with Alcian Blue and were associated with columnarcells. K corpuscles were oval, dark-brown multilamellar bodies and were associatedwith pyramidal cells. Under TEM, C corpuscles occurred within vacuoles of columnarcells and contained many electron-dense clumps and irregular membrane stacks andvesicles spread in an electron-lucent matrix. Sometimes a membrane appeared detachedfrom the inner surface of the wall, suggesting the existence of a plasma membrane. Inturn, K corpuscles were contained within vacuoles of pyramidal cells and were madeof concentric lamellae, which were in turn made of an electron-dense fibrogranularmaterial. No membranes were seen in them. Interspecifically, C corpuscles varysignificantly in width and inner contents. K corpuscles were also variable in lengthand width. However, both C and K corpuscles in the three studied species hybridisedwith generalised cyanobacterial/chloroplast probes for 16S rRNA. Also, both corpuscletypes (isolated from gland homogenates) were sensitive to lysozyme digestion, whichindicates that bacterial peptidoglycans are an integral part of their covers. The reporteddata confirm and extend previous studies on P. canaliculata in which the endosymbioticnature of C and K corpuscles were first proposed. We further propose that theendosymbiotic corpuscles are related to the Cyanobacteria/chloroplasts clade. Basedon the known distribution of these corpuscles in the major clades of Ampullariidae,we hypothesise they may be universally distributed in this family, and that mayconstitute an interesting model for studying the co-evolution of endosymbionts andtheir gastropod hosts.Fil: Dell Agnola, Federico Agustín. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; ArgentinaFil: Rodríguez, Cristian. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Castro Vazquez, Alfredo Juan. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; ArgentinaFil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; ArgentinaPeerJ, Inc2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/109362Dell Agnola, Federico Agustín; Rodríguez, Cristian; Castro Vazquez, Alfredo Juan; Vega, Israel Aníbal; A multiple comparative study of putative endosymbionts in three coexisting apple snail species; PeerJ, Inc; Peerj; 7; 8125; 10-2019; 1-192167-8359CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://peerj.com/articles/8125/info:eu-repo/semantics/altIdentifier/doi/10.7717/peerj.8125info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:15:38Zoai:ri.conicet.gov.ar:11336/109362instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:15:38.367CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A multiple comparative study of putative endosymbionts in three coexisting apple snail species
title A multiple comparative study of putative endosymbionts in three coexisting apple snail species
spellingShingle A multiple comparative study of putative endosymbionts in three coexisting apple snail species
Dell Agnola, Federico Agustín
AMPULLARIIDAE
POMACEA
ASOLENE
INTRACELLULAR BACTERIA
ENDOSYMBIOSIS
COEVOLUTION
DIGESTIVE GLAND
MORPHOMETRY
16S rRNA
FISH
LYSOZYME SENSITIVITY
title_short A multiple comparative study of putative endosymbionts in three coexisting apple snail species
title_full A multiple comparative study of putative endosymbionts in three coexisting apple snail species
title_fullStr A multiple comparative study of putative endosymbionts in three coexisting apple snail species
title_full_unstemmed A multiple comparative study of putative endosymbionts in three coexisting apple snail species
title_sort A multiple comparative study of putative endosymbionts in three coexisting apple snail species
dc.creator.none.fl_str_mv Dell Agnola, Federico Agustín
Rodríguez, Cristian
Castro Vazquez, Alfredo Juan
Vega, Israel Aníbal
author Dell Agnola, Federico Agustín
author_facet Dell Agnola, Federico Agustín
Rodríguez, Cristian
Castro Vazquez, Alfredo Juan
Vega, Israel Aníbal
author_role author
author2 Rodríguez, Cristian
Castro Vazquez, Alfredo Juan
Vega, Israel Aníbal
author2_role author
author
author
dc.subject.none.fl_str_mv AMPULLARIIDAE
POMACEA
ASOLENE
INTRACELLULAR BACTERIA
ENDOSYMBIOSIS
COEVOLUTION
DIGESTIVE GLAND
MORPHOMETRY
16S rRNA
FISH
LYSOZYME SENSITIVITY
topic AMPULLARIIDAE
POMACEA
ASOLENE
INTRACELLULAR BACTERIA
ENDOSYMBIOSIS
COEVOLUTION
DIGESTIVE GLAND
MORPHOMETRY
16S rRNA
FISH
LYSOZYME SENSITIVITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We here compare morphological and molecular characters of some putative endosymbioticelements of the digestive gland of three ampullariid species (Pomacea canaliculata,Pomacea scalaris and Asolene platae) which coexist in Lake Regatas (Palermo, BuenosAires). The putative endosymbionts were reported in these species and were identifiedas C and K corpuscles. The three species show tubuloacinar glands, each adenomerewas constituted mainly by two distinct cell types (columnar and pyramidal). C and Kcorpuscles together occupied from one-fourth to one-fifth of the tissue area in the threehost species, where C corpuscles were round and greenish-brown, were delimited bya distinct wall, stained positively with Alcian Blue and were associated with columnarcells. K corpuscles were oval, dark-brown multilamellar bodies and were associatedwith pyramidal cells. Under TEM, C corpuscles occurred within vacuoles of columnarcells and contained many electron-dense clumps and irregular membrane stacks andvesicles spread in an electron-lucent matrix. Sometimes a membrane appeared detachedfrom the inner surface of the wall, suggesting the existence of a plasma membrane. Inturn, K corpuscles were contained within vacuoles of pyramidal cells and were madeof concentric lamellae, which were in turn made of an electron-dense fibrogranularmaterial. No membranes were seen in them. Interspecifically, C corpuscles varysignificantly in width and inner contents. K corpuscles were also variable in lengthand width. However, both C and K corpuscles in the three studied species hybridisedwith generalised cyanobacterial/chloroplast probes for 16S rRNA. Also, both corpuscletypes (isolated from gland homogenates) were sensitive to lysozyme digestion, whichindicates that bacterial peptidoglycans are an integral part of their covers. The reporteddata confirm and extend previous studies on P. canaliculata in which the endosymbioticnature of C and K corpuscles were first proposed. We further propose that theendosymbiotic corpuscles are related to the Cyanobacteria/chloroplasts clade. Basedon the known distribution of these corpuscles in the major clades of Ampullariidae,we hypothesise they may be universally distributed in this family, and that mayconstitute an interesting model for studying the co-evolution of endosymbionts andtheir gastropod hosts.
Fil: Dell Agnola, Federico Agustín. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: Rodríguez, Cristian. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Castro Vazquez, Alfredo Juan. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Fisiologia; Argentina
description We here compare morphological and molecular characters of some putative endosymbioticelements of the digestive gland of three ampullariid species (Pomacea canaliculata,Pomacea scalaris and Asolene platae) which coexist in Lake Regatas (Palermo, BuenosAires). The putative endosymbionts were reported in these species and were identifiedas C and K corpuscles. The three species show tubuloacinar glands, each adenomerewas constituted mainly by two distinct cell types (columnar and pyramidal). C and Kcorpuscles together occupied from one-fourth to one-fifth of the tissue area in the threehost species, where C corpuscles were round and greenish-brown, were delimited bya distinct wall, stained positively with Alcian Blue and were associated with columnarcells. K corpuscles were oval, dark-brown multilamellar bodies and were associatedwith pyramidal cells. Under TEM, C corpuscles occurred within vacuoles of columnarcells and contained many electron-dense clumps and irregular membrane stacks andvesicles spread in an electron-lucent matrix. Sometimes a membrane appeared detachedfrom the inner surface of the wall, suggesting the existence of a plasma membrane. Inturn, K corpuscles were contained within vacuoles of pyramidal cells and were madeof concentric lamellae, which were in turn made of an electron-dense fibrogranularmaterial. No membranes were seen in them. Interspecifically, C corpuscles varysignificantly in width and inner contents. K corpuscles were also variable in lengthand width. However, both C and K corpuscles in the three studied species hybridisedwith generalised cyanobacterial/chloroplast probes for 16S rRNA. Also, both corpuscletypes (isolated from gland homogenates) were sensitive to lysozyme digestion, whichindicates that bacterial peptidoglycans are an integral part of their covers. The reporteddata confirm and extend previous studies on P. canaliculata in which the endosymbioticnature of C and K corpuscles were first proposed. We further propose that theendosymbiotic corpuscles are related to the Cyanobacteria/chloroplasts clade. Basedon the known distribution of these corpuscles in the major clades of Ampullariidae,we hypothesise they may be universally distributed in this family, and that mayconstitute an interesting model for studying the co-evolution of endosymbionts andtheir gastropod hosts.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/109362
Dell Agnola, Federico Agustín; Rodríguez, Cristian; Castro Vazquez, Alfredo Juan; Vega, Israel Aníbal; A multiple comparative study of putative endosymbionts in three coexisting apple snail species; PeerJ, Inc; Peerj; 7; 8125; 10-2019; 1-19
2167-8359
CONICET Digital
CONICET
url http://hdl.handle.net/11336/109362
identifier_str_mv Dell Agnola, Federico Agustín; Rodríguez, Cristian; Castro Vazquez, Alfredo Juan; Vega, Israel Aníbal; A multiple comparative study of putative endosymbionts in three coexisting apple snail species; PeerJ, Inc; Peerj; 7; 8125; 10-2019; 1-19
2167-8359
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://peerj.com/articles/8125/
info:eu-repo/semantics/altIdentifier/doi/10.7717/peerj.8125
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv PeerJ, Inc
publisher.none.fl_str_mv PeerJ, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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