Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chip

Autores
Toderi Cicchini, Martín Alejandro; Castellini, Horacio Virginio; Riquelme, Bibiana Doris
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The study of red blood cell (RBC) aggregation is of great interest because of its implications for human health. Altered RBC aggregation can lead to microcirculatory problems as in vascular pathologies, such as hypertension and diabetes, due to a decrease in the erythrocyte surface electric charge and an increase in the ligands present in plasma. The process of erythrocyte aggregation was studied in stasis situation (free shear stresses), using an optical chip based on the laser transmission technique. Kinetic curves of erythrocyte aggregation under different conditions were obtained, allowing evaluation and characterization of this process. Two main characteristics of blood that influence erythrocyte aggregation were analyzed: the erythrocyte surface anionic charge (EAC) after digestion with the enzyme trypsin and plasmatic protein concentration in suspension medium using plasma dissolutions in physiological saline with human albumin. A theoretical approach was evaluated to obtain aggregation and disaggregation ratios by syllectograms data fitting. Sensible parameters ( Amp 100 , t 1 \ 2 ) regarding a reduced erythrocyte EAC were determined, and other parameters (AI, M-Index) resulted that are representative of a variation in the plasmatic protein content of the suspension medium. These results are very useful for further applications in biomedicine.
Fil: Toderi Cicchini, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina
Fil: Castellini, Horacio Virginio. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina
Fil: Riquelme, Bibiana Doris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Materia
LASER TRANSMISSION
ERYTHROCYTE AGGREGATION
HEMORHEOLOGY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53234
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/53234
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chipToderi Cicchini, Martín AlejandroCastellini, Horacio VirginioRiquelme, Bibiana DorisLASER TRANSMISSIONERYTHROCYTE AGGREGATIONHEMORHEOLOGYhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The study of red blood cell (RBC) aggregation is of great interest because of its implications for human health. Altered RBC aggregation can lead to microcirculatory problems as in vascular pathologies, such as hypertension and diabetes, due to a decrease in the erythrocyte surface electric charge and an increase in the ligands present in plasma. The process of erythrocyte aggregation was studied in stasis situation (free shear stresses), using an optical chip based on the laser transmission technique. Kinetic curves of erythrocyte aggregation under different conditions were obtained, allowing evaluation and characterization of this process. Two main characteristics of blood that influence erythrocyte aggregation were analyzed: the erythrocyte surface anionic charge (EAC) after digestion with the enzyme trypsin and plasmatic protein concentration in suspension medium using plasma dissolutions in physiological saline with human albumin. A theoretical approach was evaluated to obtain aggregation and disaggregation ratios by syllectograms data fitting. Sensible parameters ( Amp 100 , t 1 \ 2 ) regarding a reduced erythrocyte EAC were determined, and other parameters (AI, M-Index) resulted that are representative of a variation in the plasmatic protein content of the suspension medium. These results are very useful for further applications in biomedicine.Fil: Toderi Cicchini, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; ArgentinaFil: Castellini, Horacio Virginio. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; ArgentinaFil: Riquelme, Bibiana Doris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaSociety of Photo-Optical Instrumentation Engineers2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53234Toderi Cicchini, Martín Alejandro; Castellini, Horacio Virginio; Riquelme, Bibiana Doris; Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chip; Society of Photo-Optical Instrumentation Engineers; Journal Of Biomedical Optics; 22; 1; 1-2017; 017003-1-017003-81083-3668CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1117/1.JBO.22.1.017003info:eu-repo/semantics/altIdentifier/url/https://www.spiedigitallibrary.org/journals/Journal-of-Biomedical-Optics/volume-22/issue-1/017003/Descriptive-parameters-of-the-erythrocyte-aggregation-phenomenon-using-a-laser/10.1117/1.JBO.22.1.017003.short?SSO=1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:12:00Zoai:ri.conicet.gov.ar:11336/53234instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:12:00.844CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chip
title Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chip
spellingShingle Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chip
Toderi Cicchini, Martín Alejandro
LASER TRANSMISSION
ERYTHROCYTE AGGREGATION
HEMORHEOLOGY
title_short Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chip
title_full Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chip
title_fullStr Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chip
title_full_unstemmed Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chip
title_sort Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chip
dc.creator.none.fl_str_mv Toderi Cicchini, Martín Alejandro
Castellini, Horacio Virginio
Riquelme, Bibiana Doris
author Toderi Cicchini, Martín Alejandro
author_facet Toderi Cicchini, Martín Alejandro
Castellini, Horacio Virginio
Riquelme, Bibiana Doris
author_role author
author2 Castellini, Horacio Virginio
Riquelme, Bibiana Doris
author2_role author
author
dc.subject.none.fl_str_mv LASER TRANSMISSION
ERYTHROCYTE AGGREGATION
HEMORHEOLOGY
topic LASER TRANSMISSION
ERYTHROCYTE AGGREGATION
HEMORHEOLOGY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The study of red blood cell (RBC) aggregation is of great interest because of its implications for human health. Altered RBC aggregation can lead to microcirculatory problems as in vascular pathologies, such as hypertension and diabetes, due to a decrease in the erythrocyte surface electric charge and an increase in the ligands present in plasma. The process of erythrocyte aggregation was studied in stasis situation (free shear stresses), using an optical chip based on the laser transmission technique. Kinetic curves of erythrocyte aggregation under different conditions were obtained, allowing evaluation and characterization of this process. Two main characteristics of blood that influence erythrocyte aggregation were analyzed: the erythrocyte surface anionic charge (EAC) after digestion with the enzyme trypsin and plasmatic protein concentration in suspension medium using plasma dissolutions in physiological saline with human albumin. A theoretical approach was evaluated to obtain aggregation and disaggregation ratios by syllectograms data fitting. Sensible parameters ( Amp 100 , t 1 \ 2 ) regarding a reduced erythrocyte EAC were determined, and other parameters (AI, M-Index) resulted that are representative of a variation in the plasmatic protein content of the suspension medium. These results are very useful for further applications in biomedicine.
Fil: Toderi Cicchini, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina
Fil: Castellini, Horacio Virginio. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina
Fil: Riquelme, Bibiana Doris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
description The study of red blood cell (RBC) aggregation is of great interest because of its implications for human health. Altered RBC aggregation can lead to microcirculatory problems as in vascular pathologies, such as hypertension and diabetes, due to a decrease in the erythrocyte surface electric charge and an increase in the ligands present in plasma. The process of erythrocyte aggregation was studied in stasis situation (free shear stresses), using an optical chip based on the laser transmission technique. Kinetic curves of erythrocyte aggregation under different conditions were obtained, allowing evaluation and characterization of this process. Two main characteristics of blood that influence erythrocyte aggregation were analyzed: the erythrocyte surface anionic charge (EAC) after digestion with the enzyme trypsin and plasmatic protein concentration in suspension medium using plasma dissolutions in physiological saline with human albumin. A theoretical approach was evaluated to obtain aggregation and disaggregation ratios by syllectograms data fitting. Sensible parameters ( Amp 100 , t 1 \ 2 ) regarding a reduced erythrocyte EAC were determined, and other parameters (AI, M-Index) resulted that are representative of a variation in the plasmatic protein content of the suspension medium. These results are very useful for further applications in biomedicine.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53234
Toderi Cicchini, Martín Alejandro; Castellini, Horacio Virginio; Riquelme, Bibiana Doris; Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chip; Society of Photo-Optical Instrumentation Engineers; Journal Of Biomedical Optics; 22; 1; 1-2017; 017003-1-017003-8
1083-3668
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53234
identifier_str_mv Toderi Cicchini, Martín Alejandro; Castellini, Horacio Virginio; Riquelme, Bibiana Doris; Descriptive parameters of the erythrocyte aggregation phenomenon using a laser transmission optical chip; Society of Photo-Optical Instrumentation Engineers; Journal Of Biomedical Optics; 22; 1; 1-2017; 017003-1-017003-8
1083-3668
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1117/1.JBO.22.1.017003
info:eu-repo/semantics/altIdentifier/url/https://www.spiedigitallibrary.org/journals/Journal-of-Biomedical-Optics/volume-22/issue-1/017003/Descriptive-parameters-of-the-erythrocyte-aggregation-phenomenon-using-a-laser/10.1117/1.JBO.22.1.017003.short?SSO=1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society of Photo-Optical Instrumentation Engineers
publisher.none.fl_str_mv Society of Photo-Optical Instrumentation Engineers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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