Red blood cell alterations by in vitro action of Trichinella spiralis newborn larvae

Autores
Ponce de León, Patricia Carolina; Toderi Cicchini, Martín Alejandro; Castellini, Horacio Virginio; Riquelme, Bibiana Doris
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: T. spiralis establishes an intimate contact with the host erythrocytes during the newborn larvae migration through the bloodstream to their encystment in the muscle. Objective: In the present work we study the alterations in the mechanical and aggregation properties of red blood cells produced in Vitro by newborn larvae at low concentrations (100, 250, 500, and 1000 larvae/mL). Methods: The study was performed by incubating human erythrocytes with an equal volume of different concentrations of newborn larvae for 30 minutes, with controlled agitation. To evaluate the erythrocyte alterations by the action of the larvae, the Erythrocyte Rheometer, the Optical Chip Aggregometer, and the Digital Image Analysis were used. Results: In the treated erythrocyte samples, a decrease in isolated cell numbers and an increase in the aggregates were observed respect to the control at the higher larval concentration (1000 larvae/mL). The deformability index, the elastic modulus and surface viscosity showed no significant differences with the control; however, the storage modulus of the erythrocyte membrane decreased significantly with the increase in larval concentration. Erythrocyte aggregation parameters showed that the aggregation index increased with the larvae concentration present in the incubation, highlighting the presence of larger aggregates and clusters. The time to attain half of the maximum aggregation diminished, which implies faster aggregation kinetics. Conclusion: The results observed in Vitro describe the red blood cell alterations that could be produced during in vivo infection in the host.
Fil: Ponce de León, Patricia Carolina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Toderi Cicchini, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Castellini, Horacio Virginio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina
Fil: Riquelme, Bibiana Doris. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina
Materia
Trichinella Spirallis
Red Blood Cells
Erythrocyte viscoelasticity
Hemorheology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/144167
Acceder
id CONICETDig_d1afda27ef14989e2b58b7d9d1c51020
oai_identifier_str oai:ri.conicet.gov.ar:11336/144167
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Red blood cell alterations by in vitro action of Trichinella spiralis newborn larvaePonce de León, Patricia CarolinaToderi Cicchini, Martín AlejandroCastellini, Horacio VirginioRiquelme, Bibiana DorisTrichinella SpirallisRed Blood CellsErythrocyte viscoelasticityHemorheologyhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1Background: T. spiralis establishes an intimate contact with the host erythrocytes during the newborn larvae migration through the bloodstream to their encystment in the muscle. Objective: In the present work we study the alterations in the mechanical and aggregation properties of red blood cells produced in Vitro by newborn larvae at low concentrations (100, 250, 500, and 1000 larvae/mL). Methods: The study was performed by incubating human erythrocytes with an equal volume of different concentrations of newborn larvae for 30 minutes, with controlled agitation. To evaluate the erythrocyte alterations by the action of the larvae, the Erythrocyte Rheometer, the Optical Chip Aggregometer, and the Digital Image Analysis were used. Results: In the treated erythrocyte samples, a decrease in isolated cell numbers and an increase in the aggregates were observed respect to the control at the higher larval concentration (1000 larvae/mL). The deformability index, the elastic modulus and surface viscosity showed no significant differences with the control; however, the storage modulus of the erythrocyte membrane decreased significantly with the increase in larval concentration. Erythrocyte aggregation parameters showed that the aggregation index increased with the larvae concentration present in the incubation, highlighting the presence of larger aggregates and clusters. The time to attain half of the maximum aggregation diminished, which implies faster aggregation kinetics. Conclusion: The results observed in Vitro describe the red blood cell alterations that could be produced during in vivo infection in the host.Fil: Ponce de León, Patricia Carolina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Toderi Cicchini, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Castellini, Horacio Virginio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; ArgentinaFil: Riquelme, Bibiana Doris. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; ArgentinaPeertechz2020-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/144167Ponce de León, Patricia Carolina; Toderi Cicchini, Martín Alejandro; Castellini, Horacio Virginio; Riquelme, Bibiana Doris; Red blood cell alterations by in vitro action of Trichinella spiralis newborn larvae; Peertechz; Global Journal of Biotechnology and Biomaterial Science; 6; 1; 5-2020; 7-122640-7817CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.peertechz.com/articles/GJBBS-6-112.phpinfo:eu-repo/semantics/altIdentifier/doi/10.17352/gjbbs.000012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:33Zoai:ri.conicet.gov.ar:11336/144167instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:33.844CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Red blood cell alterations by in vitro action of Trichinella spiralis newborn larvae
title Red blood cell alterations by in vitro action of Trichinella spiralis newborn larvae
spellingShingle Red blood cell alterations by in vitro action of Trichinella spiralis newborn larvae
Ponce de León, Patricia Carolina
Trichinella Spirallis
Red Blood Cells
Erythrocyte viscoelasticity
Hemorheology
title_short Red blood cell alterations by in vitro action of Trichinella spiralis newborn larvae
title_full Red blood cell alterations by in vitro action of Trichinella spiralis newborn larvae
title_fullStr Red blood cell alterations by in vitro action of Trichinella spiralis newborn larvae
title_full_unstemmed Red blood cell alterations by in vitro action of Trichinella spiralis newborn larvae
title_sort Red blood cell alterations by in vitro action of Trichinella spiralis newborn larvae
dc.creator.none.fl_str_mv Ponce de León, Patricia Carolina
Toderi Cicchini, Martín Alejandro
Castellini, Horacio Virginio
Riquelme, Bibiana Doris
author Ponce de León, Patricia Carolina
author_facet Ponce de León, Patricia Carolina
Toderi Cicchini, Martín Alejandro
Castellini, Horacio Virginio
Riquelme, Bibiana Doris
author_role author
author2 Toderi Cicchini, Martín Alejandro
Castellini, Horacio Virginio
Riquelme, Bibiana Doris
author2_role author
author
author
dc.subject.none.fl_str_mv Trichinella Spirallis
Red Blood Cells
Erythrocyte viscoelasticity
Hemorheology
topic Trichinella Spirallis
Red Blood Cells
Erythrocyte viscoelasticity
Hemorheology
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: T. spiralis establishes an intimate contact with the host erythrocytes during the newborn larvae migration through the bloodstream to their encystment in the muscle. Objective: In the present work we study the alterations in the mechanical and aggregation properties of red blood cells produced in Vitro by newborn larvae at low concentrations (100, 250, 500, and 1000 larvae/mL). Methods: The study was performed by incubating human erythrocytes with an equal volume of different concentrations of newborn larvae for 30 minutes, with controlled agitation. To evaluate the erythrocyte alterations by the action of the larvae, the Erythrocyte Rheometer, the Optical Chip Aggregometer, and the Digital Image Analysis were used. Results: In the treated erythrocyte samples, a decrease in isolated cell numbers and an increase in the aggregates were observed respect to the control at the higher larval concentration (1000 larvae/mL). The deformability index, the elastic modulus and surface viscosity showed no significant differences with the control; however, the storage modulus of the erythrocyte membrane decreased significantly with the increase in larval concentration. Erythrocyte aggregation parameters showed that the aggregation index increased with the larvae concentration present in the incubation, highlighting the presence of larger aggregates and clusters. The time to attain half of the maximum aggregation diminished, which implies faster aggregation kinetics. Conclusion: The results observed in Vitro describe the red blood cell alterations that could be produced during in vivo infection in the host.
Fil: Ponce de León, Patricia Carolina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Toderi Cicchini, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Castellini, Horacio Virginio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Física de Rosario. Universidad Nacional de Rosario. Instituto de Física de Rosario; Argentina
Fil: Riquelme, Bibiana Doris. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina
description Background: T. spiralis establishes an intimate contact with the host erythrocytes during the newborn larvae migration through the bloodstream to their encystment in the muscle. Objective: In the present work we study the alterations in the mechanical and aggregation properties of red blood cells produced in Vitro by newborn larvae at low concentrations (100, 250, 500, and 1000 larvae/mL). Methods: The study was performed by incubating human erythrocytes with an equal volume of different concentrations of newborn larvae for 30 minutes, with controlled agitation. To evaluate the erythrocyte alterations by the action of the larvae, the Erythrocyte Rheometer, the Optical Chip Aggregometer, and the Digital Image Analysis were used. Results: In the treated erythrocyte samples, a decrease in isolated cell numbers and an increase in the aggregates were observed respect to the control at the higher larval concentration (1000 larvae/mL). The deformability index, the elastic modulus and surface viscosity showed no significant differences with the control; however, the storage modulus of the erythrocyte membrane decreased significantly with the increase in larval concentration. Erythrocyte aggregation parameters showed that the aggregation index increased with the larvae concentration present in the incubation, highlighting the presence of larger aggregates and clusters. The time to attain half of the maximum aggregation diminished, which implies faster aggregation kinetics. Conclusion: The results observed in Vitro describe the red blood cell alterations that could be produced during in vivo infection in the host.
publishDate 2020
dc.date.none.fl_str_mv 2020-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/144167
Ponce de León, Patricia Carolina; Toderi Cicchini, Martín Alejandro; Castellini, Horacio Virginio; Riquelme, Bibiana Doris; Red blood cell alterations by in vitro action of Trichinella spiralis newborn larvae; Peertechz; Global Journal of Biotechnology and Biomaterial Science; 6; 1; 5-2020; 7-12
2640-7817
CONICET Digital
CONICET
url http://hdl.handle.net/11336/144167
identifier_str_mv Ponce de León, Patricia Carolina; Toderi Cicchini, Martín Alejandro; Castellini, Horacio Virginio; Riquelme, Bibiana Doris; Red blood cell alterations by in vitro action of Trichinella spiralis newborn larvae; Peertechz; Global Journal of Biotechnology and Biomaterial Science; 6; 1; 5-2020; 7-12
2640-7817
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.peertechz.com/articles/GJBBS-6-112.php
info:eu-repo/semantics/altIdentifier/doi/10.17352/gjbbs.000012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Peertechz
publisher.none.fl_str_mv Peertechz
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269527087251456
score 13.13397

Publicaciones similares