Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role
- Autores
- Luquita, A.; Urli, L.; Svetaz, M. J.; Gennaro, Ana Maria; Volpintesta, R.; Palatnik, S.; Rasia, M.
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- ncrease in erythrocyte aggregation (EA) is pathognomonic for rheumatoid arthritis (RA), and its estimation through erythrocyte sedimentation rate (ESR) is part of DAS 28-4 activity diagnosis, with low correlation with EA and that does not discriminate the contribution of cell factors that increase aggregation. Objective: To analyse cell and plasma factors that might be involved in EA increase, to understand how RA affects blood components, thus modifying blood fluid behavior. Methodology: One hundred women presenting active RA were compared with age-matched controls (C). EA was measured by transmitted light, obtaining two parameters: 2k2n0, characterizing the aggregation process kinetics and s0/n0, estimating aggregates size. Cell factors assays: erythrocyte deformability, by filtration through nucleopore membranes, cell shape, by microscopy, and membrane fluidity by EPR. Plasma: total proteins and CRP, albumin, fibrinogen (Fb), by gravimetry, and IgG and IgM by single radial immuno-diffusion. Results: AR and C (x ± SE). 2k2n0: 31.83 ± 2.84, 23.75 ± 1.91; s0/n0: 0.92 ± 0.05, 0.87 ± 0.04. Rigidity index (RI): 14.79 ± 4.71, 6.92 ± 1.31. Morphological index: 0.28 ± 0.03, 0.30 ± 0.05, n.s. Fb (mg/dl): 382 ± 80, 299 ± 70. IgG (mg/dl): 1580 ± 219, 1296 ± 158; IgM (mg/dl) 233 ± 28, 183 ± 23; albumin (g/dl) 3.84 ± 0.44, 3.77 ± 0.51 n.s. p < 0.05 accepted. Correlations: 2k2n0 vs. Fb r = 0.66; s0/n0 vs. Fb r = 0.51; 2k2n0 vs. Igs r = 0.65; s0/n0 vs. Igs r = 0.56. 2k2n0 vs. RI r = -0.59; s0/n0 vs. RI = -0.52, p < 0.05. Conclusions: Plasma factors, Igs and Fb increased aggregation, since RI is altered, this reduces the process efficiency regarding aggregation. Patients with active RA present an increased EA, with values modifications associated with the activity index DAS 28-4, thus becoming an RA activity indicator.
Fil: Luquita, A.. Universidad Nacional de Rosario; Argentina
Fil: Urli, L.. Universidad Nacional de Rosario; Argentina
Fil: Svetaz, M. J.. Universidad Nacional de Rosario; Argentina
Fil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Volpintesta, R.. Universidad Nacional de Rosario; Argentina
Fil: Palatnik, S.. Universidad Nacional de Rosario; Argentina
Fil: Rasia, M.. Universidad Nacional de Rosario; Argentina - Materia
-
Rheumatoid Arthritis Activity
Erythrocyte Aggregation
Membrane Fluidity
Erythrocyte Deformability - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/17122
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Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s roleLuquita, A.Urli, L.Svetaz, M. J.Gennaro, Ana MariaVolpintesta, R.Palatnik, S.Rasia, M.Rheumatoid Arthritis ActivityErythrocyte AggregationMembrane FluidityErythrocyte Deformabilityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1ncrease in erythrocyte aggregation (EA) is pathognomonic for rheumatoid arthritis (RA), and its estimation through erythrocyte sedimentation rate (ESR) is part of DAS 28-4 activity diagnosis, with low correlation with EA and that does not discriminate the contribution of cell factors that increase aggregation. Objective: To analyse cell and plasma factors that might be involved in EA increase, to understand how RA affects blood components, thus modifying blood fluid behavior. Methodology: One hundred women presenting active RA were compared with age-matched controls (C). EA was measured by transmitted light, obtaining two parameters: 2k2n0, characterizing the aggregation process kinetics and s0/n0, estimating aggregates size. Cell factors assays: erythrocyte deformability, by filtration through nucleopore membranes, cell shape, by microscopy, and membrane fluidity by EPR. Plasma: total proteins and CRP, albumin, fibrinogen (Fb), by gravimetry, and IgG and IgM by single radial immuno-diffusion. Results: AR and C (x ± SE). 2k2n0: 31.83 ± 2.84, 23.75 ± 1.91; s0/n0: 0.92 ± 0.05, 0.87 ± 0.04. Rigidity index (RI): 14.79 ± 4.71, 6.92 ± 1.31. Morphological index: 0.28 ± 0.03, 0.30 ± 0.05, n.s. Fb (mg/dl): 382 ± 80, 299 ± 70. IgG (mg/dl): 1580 ± 219, 1296 ± 158; IgM (mg/dl) 233 ± 28, 183 ± 23; albumin (g/dl) 3.84 ± 0.44, 3.77 ± 0.51 n.s. p < 0.05 accepted. Correlations: 2k2n0 vs. Fb r = 0.66; s0/n0 vs. Fb r = 0.51; 2k2n0 vs. Igs r = 0.65; s0/n0 vs. Igs r = 0.56. 2k2n0 vs. RI r = -0.59; s0/n0 vs. RI = -0.52, p < 0.05. Conclusions: Plasma factors, Igs and Fb increased aggregation, since RI is altered, this reduces the process efficiency regarding aggregation. Patients with active RA present an increased EA, with values modifications associated with the activity index DAS 28-4, thus becoming an RA activity indicator.Fil: Luquita, A.. Universidad Nacional de Rosario; ArgentinaFil: Urli, L.. Universidad Nacional de Rosario; ArgentinaFil: Svetaz, M. J.. Universidad Nacional de Rosario; ArgentinaFil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Volpintesta, R.. Universidad Nacional de Rosario; ArgentinaFil: Palatnik, S.. Universidad Nacional de Rosario; ArgentinaFil: Rasia, M.. Universidad Nacional de Rosario; ArgentinaIos Press2009-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17122Luquita, A.; Urli, L.; Svetaz, M. J.; Gennaro, Ana Maria; Volpintesta, R.; et al.; Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role; Ios Press; Clinical Hemorheology And Microcirculation; 41; 1; 10-2009; 49-561386-0291enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:26Zoai:ri.conicet.gov.ar:11336/17122instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:27.053CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role |
title |
Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role |
spellingShingle |
Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role Luquita, A. Rheumatoid Arthritis Activity Erythrocyte Aggregation Membrane Fluidity Erythrocyte Deformability |
title_short |
Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role |
title_full |
Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role |
title_fullStr |
Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role |
title_full_unstemmed |
Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role |
title_sort |
Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role |
dc.creator.none.fl_str_mv |
Luquita, A. Urli, L. Svetaz, M. J. Gennaro, Ana Maria Volpintesta, R. Palatnik, S. Rasia, M. |
author |
Luquita, A. |
author_facet |
Luquita, A. Urli, L. Svetaz, M. J. Gennaro, Ana Maria Volpintesta, R. Palatnik, S. Rasia, M. |
author_role |
author |
author2 |
Urli, L. Svetaz, M. J. Gennaro, Ana Maria Volpintesta, R. Palatnik, S. Rasia, M. |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Rheumatoid Arthritis Activity Erythrocyte Aggregation Membrane Fluidity Erythrocyte Deformability |
topic |
Rheumatoid Arthritis Activity Erythrocyte Aggregation Membrane Fluidity Erythrocyte Deformability |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
ncrease in erythrocyte aggregation (EA) is pathognomonic for rheumatoid arthritis (RA), and its estimation through erythrocyte sedimentation rate (ESR) is part of DAS 28-4 activity diagnosis, with low correlation with EA and that does not discriminate the contribution of cell factors that increase aggregation. Objective: To analyse cell and plasma factors that might be involved in EA increase, to understand how RA affects blood components, thus modifying blood fluid behavior. Methodology: One hundred women presenting active RA were compared with age-matched controls (C). EA was measured by transmitted light, obtaining two parameters: 2k2n0, characterizing the aggregation process kinetics and s0/n0, estimating aggregates size. Cell factors assays: erythrocyte deformability, by filtration through nucleopore membranes, cell shape, by microscopy, and membrane fluidity by EPR. Plasma: total proteins and CRP, albumin, fibrinogen (Fb), by gravimetry, and IgG and IgM by single radial immuno-diffusion. Results: AR and C (x ± SE). 2k2n0: 31.83 ± 2.84, 23.75 ± 1.91; s0/n0: 0.92 ± 0.05, 0.87 ± 0.04. Rigidity index (RI): 14.79 ± 4.71, 6.92 ± 1.31. Morphological index: 0.28 ± 0.03, 0.30 ± 0.05, n.s. Fb (mg/dl): 382 ± 80, 299 ± 70. IgG (mg/dl): 1580 ± 219, 1296 ± 158; IgM (mg/dl) 233 ± 28, 183 ± 23; albumin (g/dl) 3.84 ± 0.44, 3.77 ± 0.51 n.s. p < 0.05 accepted. Correlations: 2k2n0 vs. Fb r = 0.66; s0/n0 vs. Fb r = 0.51; 2k2n0 vs. Igs r = 0.65; s0/n0 vs. Igs r = 0.56. 2k2n0 vs. RI r = -0.59; s0/n0 vs. RI = -0.52, p < 0.05. Conclusions: Plasma factors, Igs and Fb increased aggregation, since RI is altered, this reduces the process efficiency regarding aggregation. Patients with active RA present an increased EA, with values modifications associated with the activity index DAS 28-4, thus becoming an RA activity indicator. Fil: Luquita, A.. Universidad Nacional de Rosario; Argentina Fil: Urli, L.. Universidad Nacional de Rosario; Argentina Fil: Svetaz, M. J.. Universidad Nacional de Rosario; Argentina Fil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina Fil: Volpintesta, R.. Universidad Nacional de Rosario; Argentina Fil: Palatnik, S.. Universidad Nacional de Rosario; Argentina Fil: Rasia, M.. Universidad Nacional de Rosario; Argentina |
description |
ncrease in erythrocyte aggregation (EA) is pathognomonic for rheumatoid arthritis (RA), and its estimation through erythrocyte sedimentation rate (ESR) is part of DAS 28-4 activity diagnosis, with low correlation with EA and that does not discriminate the contribution of cell factors that increase aggregation. Objective: To analyse cell and plasma factors that might be involved in EA increase, to understand how RA affects blood components, thus modifying blood fluid behavior. Methodology: One hundred women presenting active RA were compared with age-matched controls (C). EA was measured by transmitted light, obtaining two parameters: 2k2n0, characterizing the aggregation process kinetics and s0/n0, estimating aggregates size. Cell factors assays: erythrocyte deformability, by filtration through nucleopore membranes, cell shape, by microscopy, and membrane fluidity by EPR. Plasma: total proteins and CRP, albumin, fibrinogen (Fb), by gravimetry, and IgG and IgM by single radial immuno-diffusion. Results: AR and C (x ± SE). 2k2n0: 31.83 ± 2.84, 23.75 ± 1.91; s0/n0: 0.92 ± 0.05, 0.87 ± 0.04. Rigidity index (RI): 14.79 ± 4.71, 6.92 ± 1.31. Morphological index: 0.28 ± 0.03, 0.30 ± 0.05, n.s. Fb (mg/dl): 382 ± 80, 299 ± 70. IgG (mg/dl): 1580 ± 219, 1296 ± 158; IgM (mg/dl) 233 ± 28, 183 ± 23; albumin (g/dl) 3.84 ± 0.44, 3.77 ± 0.51 n.s. p < 0.05 accepted. Correlations: 2k2n0 vs. Fb r = 0.66; s0/n0 vs. Fb r = 0.51; 2k2n0 vs. Igs r = 0.65; s0/n0 vs. Igs r = 0.56. 2k2n0 vs. RI r = -0.59; s0/n0 vs. RI = -0.52, p < 0.05. Conclusions: Plasma factors, Igs and Fb increased aggregation, since RI is altered, this reduces the process efficiency regarding aggregation. Patients with active RA present an increased EA, with values modifications associated with the activity index DAS 28-4, thus becoming an RA activity indicator. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/17122 Luquita, A.; Urli, L.; Svetaz, M. J.; Gennaro, Ana Maria; Volpintesta, R.; et al.; Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role; Ios Press; Clinical Hemorheology And Microcirculation; 41; 1; 10-2009; 49-56 1386-0291 |
url |
http://hdl.handle.net/11336/17122 |
identifier_str_mv |
Luquita, A.; Urli, L.; Svetaz, M. J.; Gennaro, Ana Maria; Volpintesta, R.; et al.; Erythrocyte aggregation in rheumatoid arthritis: cell and plasma factor’s role; Ios Press; Clinical Hemorheology And Microcirculation; 41; 1; 10-2009; 49-56 1386-0291 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Ios Press |
publisher.none.fl_str_mv |
Ios Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613582209155072 |
score |
13.070432 |