In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties

Autores
Luquita, A.; Urli, L.; Svetaz, M. J.; Gennaro, Ana Maria; Giorgietti, M.; Pistone, G.; Volpintesta, R.; Palatnik, S.; Rasia, M.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Hyaluronic acid (HA) is present in many tissues; its presence in serum may be related to certaininflammatory conditions, tissue damage, sepsis, liver malfunction and some malignancies. In the present work, ourgoal was to investigate the significance of hyaluronic acid effect on erythrocyte flow properties. Therefore weperformed in vitro experiments incubating red blood cells (RBCs) with several HA concentrations. Afterwards, in orderto corroborate the pathophysiological significance of the results obtained, we replicated the in vitro experiment with exvivo RBCs from diagnosed rheumatoid arthritis (RA) patients, a serum HA-increasing pathology.Methods: Erythrocyte deformability (by filtration through nucleopore membranes) and erythrocyte aggregability (EA)were tested on blood from healthy donors additioned with purified HA. EA was measured by transmitted light andanalyzed with a mathematical model yielding two parameters, the aggregation rate and the size of the aggregates.Conformational changes of cytoskeleton proteins were estimated by electron paramagnetic resonance spectroscopy(EPR).Results: In vitro, erythrocytes treated with HA showed increased rigidity index (RI) and reduced aggregability, situationstrongly related to the rigidization of the membrane cytoskeleton triggered by HA, as shown by EPR results. Also, asignificant correlation (r: 0.77, p Conclusions: Our results lead us to postulate the hypothesis that HA interacts with the erythrocyte surface leading tomodifications in erythrocyte rheological and flow properties, both ex vivo and in vitro.
Fil: Luquita, A.. Universidad Nacional de Rosario; Argentina
Fil: Urli, L.. Universidad Nacional de Rosario; Argentina
Fil: Svetaz, M. J.. Universidad Nacional de Rosario; Argentina
Fil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad Nacional del Litoral; Argentina
Fil: Giorgietti, M.. Universidad Nacional de Rosario; Argentina
Fil: Pistone, G.. Universidad Nacional de Rosario; Argentina
Fil: Volpintesta, R.. Universidad Nacional de Rosario; Argentina
Fil: Palatnik, S.. Universidad Nacional de Rosario; Argentina
Fil: Rasia, M.. Universidad Nacional de Rosario; Argentina
Materia
Hyaluronic acid
erythrocyte deformability
erythrocyte aggregation
rheumatoid arthritis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13757
Acceder
id CONICETDig_0770bd0ac1126b93c58d03b357dfab0a
oai_identifier_str oai:ri.conicet.gov.ar:11336/13757
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow propertiesLuquita, A.Urli, L.Svetaz, M. J.Gennaro, Ana MariaGiorgietti, M.Pistone, G.Volpintesta, R.Palatnik, S.Rasia, M.Hyaluronic aciderythrocyte deformabilityerythrocyte aggregationrheumatoid arthritishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Hyaluronic acid (HA) is present in many tissues; its presence in serum may be related to certaininflammatory conditions, tissue damage, sepsis, liver malfunction and some malignancies. In the present work, ourgoal was to investigate the significance of hyaluronic acid effect on erythrocyte flow properties. Therefore weperformed in vitro experiments incubating red blood cells (RBCs) with several HA concentrations. Afterwards, in orderto corroborate the pathophysiological significance of the results obtained, we replicated the in vitro experiment with exvivo RBCs from diagnosed rheumatoid arthritis (RA) patients, a serum HA-increasing pathology.Methods: Erythrocyte deformability (by filtration through nucleopore membranes) and erythrocyte aggregability (EA)were tested on blood from healthy donors additioned with purified HA. EA was measured by transmitted light andanalyzed with a mathematical model yielding two parameters, the aggregation rate and the size of the aggregates.Conformational changes of cytoskeleton proteins were estimated by electron paramagnetic resonance spectroscopy(EPR).Results: In vitro, erythrocytes treated with HA showed increased rigidity index (RI) and reduced aggregability, situationstrongly related to the rigidization of the membrane cytoskeleton triggered by HA, as shown by EPR results. Also, asignificant correlation (r: 0.77, p Conclusions: Our results lead us to postulate the hypothesis that HA interacts with the erythrocyte surface leading tomodifications in erythrocyte rheological and flow properties, both ex vivo and in vitro.Fil: Luquita, A.. Universidad Nacional de Rosario; ArgentinaFil: Urli, L.. Universidad Nacional de Rosario; ArgentinaFil: Svetaz, M. J.. Universidad Nacional de Rosario; ArgentinaFil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad Nacional del Litoral; ArgentinaFil: Giorgietti, M.. Universidad Nacional de Rosario; ArgentinaFil: Pistone, G.. Universidad Nacional de Rosario; ArgentinaFil: Volpintesta, R.. Universidad Nacional de Rosario; ArgentinaFil: Palatnik, S.. Universidad Nacional de Rosario; ArgentinaFil: Rasia, M.. Universidad Nacional de Rosario; ArgentinaBiomed Central2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13757Luquita, A.; Urli, L.; Svetaz, M. J.; Gennaro, Ana Maria; Giorgietti, M.; et al.; In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties; Biomed Central; Journal Of Biomedical Science; 17; 12-2010; 8-141021-7770enginfo:eu-repo/semantics/altIdentifier/url/http://jbiomedsci.biomedcentral.com/articles/10.1186/1423-0127-17-8info:eu-repo/semantics/altIdentifier/doi/10.1186/1423-0127-17-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:27Zoai:ri.conicet.gov.ar:11336/13757instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:27.412CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties
title In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties
spellingShingle In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties
Luquita, A.
Hyaluronic acid
erythrocyte deformability
erythrocyte aggregation
rheumatoid arthritis
title_short In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties
title_full In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties
title_fullStr In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties
title_full_unstemmed In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties
title_sort In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties
dc.creator.none.fl_str_mv Luquita, A.
Urli, L.
Svetaz, M. J.
Gennaro, Ana Maria
Giorgietti, M.
Pistone, G.
Volpintesta, R.
Palatnik, S.
Rasia, M.
author Luquita, A.
author_facet Luquita, A.
Urli, L.
Svetaz, M. J.
Gennaro, Ana Maria
Giorgietti, M.
Pistone, G.
Volpintesta, R.
Palatnik, S.
Rasia, M.
author_role author
author2 Urli, L.
Svetaz, M. J.
Gennaro, Ana Maria
Giorgietti, M.
Pistone, G.
Volpintesta, R.
Palatnik, S.
Rasia, M.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Hyaluronic acid
erythrocyte deformability
erythrocyte aggregation
rheumatoid arthritis
topic Hyaluronic acid
erythrocyte deformability
erythrocyte aggregation
rheumatoid arthritis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Hyaluronic acid (HA) is present in many tissues; its presence in serum may be related to certaininflammatory conditions, tissue damage, sepsis, liver malfunction and some malignancies. In the present work, ourgoal was to investigate the significance of hyaluronic acid effect on erythrocyte flow properties. Therefore weperformed in vitro experiments incubating red blood cells (RBCs) with several HA concentrations. Afterwards, in orderto corroborate the pathophysiological significance of the results obtained, we replicated the in vitro experiment with exvivo RBCs from diagnosed rheumatoid arthritis (RA) patients, a serum HA-increasing pathology.Methods: Erythrocyte deformability (by filtration through nucleopore membranes) and erythrocyte aggregability (EA)were tested on blood from healthy donors additioned with purified HA. EA was measured by transmitted light andanalyzed with a mathematical model yielding two parameters, the aggregation rate and the size of the aggregates.Conformational changes of cytoskeleton proteins were estimated by electron paramagnetic resonance spectroscopy(EPR).Results: In vitro, erythrocytes treated with HA showed increased rigidity index (RI) and reduced aggregability, situationstrongly related to the rigidization of the membrane cytoskeleton triggered by HA, as shown by EPR results. Also, asignificant correlation (r: 0.77, p Conclusions: Our results lead us to postulate the hypothesis that HA interacts with the erythrocyte surface leading tomodifications in erythrocyte rheological and flow properties, both ex vivo and in vitro.
Fil: Luquita, A.. Universidad Nacional de Rosario; Argentina
Fil: Urli, L.. Universidad Nacional de Rosario; Argentina
Fil: Svetaz, M. J.. Universidad Nacional de Rosario; Argentina
Fil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad Nacional del Litoral; Argentina
Fil: Giorgietti, M.. Universidad Nacional de Rosario; Argentina
Fil: Pistone, G.. Universidad Nacional de Rosario; Argentina
Fil: Volpintesta, R.. Universidad Nacional de Rosario; Argentina
Fil: Palatnik, S.. Universidad Nacional de Rosario; Argentina
Fil: Rasia, M.. Universidad Nacional de Rosario; Argentina
description Background: Hyaluronic acid (HA) is present in many tissues; its presence in serum may be related to certaininflammatory conditions, tissue damage, sepsis, liver malfunction and some malignancies. In the present work, ourgoal was to investigate the significance of hyaluronic acid effect on erythrocyte flow properties. Therefore weperformed in vitro experiments incubating red blood cells (RBCs) with several HA concentrations. Afterwards, in orderto corroborate the pathophysiological significance of the results obtained, we replicated the in vitro experiment with exvivo RBCs from diagnosed rheumatoid arthritis (RA) patients, a serum HA-increasing pathology.Methods: Erythrocyte deformability (by filtration through nucleopore membranes) and erythrocyte aggregability (EA)were tested on blood from healthy donors additioned with purified HA. EA was measured by transmitted light andanalyzed with a mathematical model yielding two parameters, the aggregation rate and the size of the aggregates.Conformational changes of cytoskeleton proteins were estimated by electron paramagnetic resonance spectroscopy(EPR).Results: In vitro, erythrocytes treated with HA showed increased rigidity index (RI) and reduced aggregability, situationstrongly related to the rigidization of the membrane cytoskeleton triggered by HA, as shown by EPR results. Also, asignificant correlation (r: 0.77, p Conclusions: Our results lead us to postulate the hypothesis that HA interacts with the erythrocyte surface leading tomodifications in erythrocyte rheological and flow properties, both ex vivo and in vitro.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13757
Luquita, A.; Urli, L.; Svetaz, M. J.; Gennaro, Ana Maria; Giorgietti, M.; et al.; In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties; Biomed Central; Journal Of Biomedical Science; 17; 12-2010; 8-14
1021-7770
url http://hdl.handle.net/11336/13757
identifier_str_mv Luquita, A.; Urli, L.; Svetaz, M. J.; Gennaro, Ana Maria; Giorgietti, M.; et al.; In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties; Biomed Central; Journal Of Biomedical Science; 17; 12-2010; 8-14
1021-7770
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jbiomedsci.biomedcentral.com/articles/10.1186/1423-0127-17-8
info:eu-repo/semantics/altIdentifier/doi/10.1186/1423-0127-17-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Biomed Central
publisher.none.fl_str_mv Biomed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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