Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells

Autores
Sepúlveda, Diana Elizabeth; Cabeza Meckert, Patricia; Locatelli, Paola; Olea, Fernanda Daniela; Perez, Nestor Gustavo; Pinilla, Oscar Andres; Diaz, Romina Gisel; Crottogini, Alberto José; Laguens, Rubén
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The adult heart contains a population of cardiac progenitor cells (CPCs). Growing and collecting an adequate number of CPCs demands complex culture media containing growth factors. Since activated macrophages secrete many growth factors, we investigated if activated isolated heart cells seeded on a feeder layer of activated peritoneal macrophages (PM) could result in CPCs and if these, in turn, could exert cardioprotection in rats with myocardial infarction (MI). Heart cells of inbred Wistar rats were isolated by collagenase digestion and cultured on PM obtained 72 h after intraperitoneal injection of 12 ml thioglycollate. Cells (1 × 106) exhibiting CPC phenotype (immunohistochemistry) were injected in the periphery of rat MI 10 min after coronary artery occlusion. Control rats received vehicle. Three weeks later, left ventricular (LV) function (echocardiogram) was assessed, animals were euthanized and the hearts removed for histological studies. Five to six days after seeding heart cells on PM, spherical clusters composed of small bright and spherical cells expressing mostly c-Kit and Sca-1 antigens were apparent. After explant, those clusters developed cobblestone-like monolayers that expressed smooth muscle actin and sarcomeric actin and were successfully transferred for more than ten passages. When injected in the MI periphery, many of them survived at 21 days after coronary ligature, improved LV ejection fraction and decreased scar size as compared with control rats. CPC-derived cells with cardiocyte and smooth muscle phenotypes can be successfully grown on a feeder layer of activated syngeneic PM. These cells decreased scar size and improved heart function in rats with MI.
Fil: Sepúlveda, Diana Elizabeth. Universidad Favaloro; Argentina
Fil: Cabeza Meckert, Patricia. Universidad Favaloro; Argentina
Fil: Locatelli, Paola. Universidad Favaloro; Argentina
Fil: Olea, Fernanda Daniela. Universidad Favaloro; Argentina
Fil: Perez, Nestor Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Fil: Pinilla, Oscar Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Fil: Diaz, Romina Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Fil: Crottogini, Alberto José. Universidad Favaloro; Argentina
Fil: Laguens, Rubén. Universidad Favaloro; Argentina
Materia
Cardiac Progenitor Cells
Macrophage
Myocardial Infarction
Rat
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36443
Acceder
id CONICETDig_81c7e32a38b6acf09614af46eb2fb140
oai_identifier_str oai:ri.conicet.gov.ar:11336/36443
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Activated macrophages as a feeder layer for growth of resident cardiac progenitor cellsSepúlveda, Diana ElizabethCabeza Meckert, PatriciaLocatelli, PaolaOlea, Fernanda DanielaPerez, Nestor GustavoPinilla, Oscar AndresDiaz, Romina GiselCrottogini, Alberto JoséLaguens, RubénCardiac Progenitor CellsMacrophageMyocardial InfarctionRathttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The adult heart contains a population of cardiac progenitor cells (CPCs). Growing and collecting an adequate number of CPCs demands complex culture media containing growth factors. Since activated macrophages secrete many growth factors, we investigated if activated isolated heart cells seeded on a feeder layer of activated peritoneal macrophages (PM) could result in CPCs and if these, in turn, could exert cardioprotection in rats with myocardial infarction (MI). Heart cells of inbred Wistar rats were isolated by collagenase digestion and cultured on PM obtained 72 h after intraperitoneal injection of 12 ml thioglycollate. Cells (1 × 106) exhibiting CPC phenotype (immunohistochemistry) were injected in the periphery of rat MI 10 min after coronary artery occlusion. Control rats received vehicle. Three weeks later, left ventricular (LV) function (echocardiogram) was assessed, animals were euthanized and the hearts removed for histological studies. Five to six days after seeding heart cells on PM, spherical clusters composed of small bright and spherical cells expressing mostly c-Kit and Sca-1 antigens were apparent. After explant, those clusters developed cobblestone-like monolayers that expressed smooth muscle actin and sarcomeric actin and were successfully transferred for more than ten passages. When injected in the MI periphery, many of them survived at 21 days after coronary ligature, improved LV ejection fraction and decreased scar size as compared with control rats. CPC-derived cells with cardiocyte and smooth muscle phenotypes can be successfully grown on a feeder layer of activated syngeneic PM. These cells decreased scar size and improved heart function in rats with MI.Fil: Sepúlveda, Diana Elizabeth. Universidad Favaloro; ArgentinaFil: Cabeza Meckert, Patricia. Universidad Favaloro; ArgentinaFil: Locatelli, Paola. Universidad Favaloro; ArgentinaFil: Olea, Fernanda Daniela. Universidad Favaloro; ArgentinaFil: Perez, Nestor Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; ArgentinaFil: Pinilla, Oscar Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; ArgentinaFil: Diaz, Romina Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; ArgentinaFil: Crottogini, Alberto José. Universidad Favaloro; ArgentinaFil: Laguens, Rubén. Universidad Favaloro; ArgentinaSpringer2014-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36443Sepúlveda, Diana Elizabeth; Cabeza Meckert, Patricia; Locatelli, Paola; Olea, Fernanda Daniela; Perez, Nestor Gustavo; et al.; Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells; Springer; Cytotechnology (dordrecht); 68; 4; 11-2014; 665-6740920-9069CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10616-014-9814-0info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10616-014-9814-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-04-08T11:22:17Zoai:ri.conicet.gov.ar:11336/36443instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-04-08 11:22:17.739CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells
title Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells
spellingShingle Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells
Sepúlveda, Diana Elizabeth
Cardiac Progenitor Cells
Macrophage
Myocardial Infarction
Rat
title_short Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells
title_full Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells
title_fullStr Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells
title_full_unstemmed Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells
title_sort Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells
dc.creator.none.fl_str_mv Sepúlveda, Diana Elizabeth
Cabeza Meckert, Patricia
Locatelli, Paola
Olea, Fernanda Daniela
Perez, Nestor Gustavo
Pinilla, Oscar Andres
Diaz, Romina Gisel
Crottogini, Alberto José
Laguens, Rubén
author Sepúlveda, Diana Elizabeth
author_facet Sepúlveda, Diana Elizabeth
Cabeza Meckert, Patricia
Locatelli, Paola
Olea, Fernanda Daniela
Perez, Nestor Gustavo
Pinilla, Oscar Andres
Diaz, Romina Gisel
Crottogini, Alberto José
Laguens, Rubén
author_role author
author2 Cabeza Meckert, Patricia
Locatelli, Paola
Olea, Fernanda Daniela
Perez, Nestor Gustavo
Pinilla, Oscar Andres
Diaz, Romina Gisel
Crottogini, Alberto José
Laguens, Rubén
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cardiac Progenitor Cells
Macrophage
Myocardial Infarction
Rat
topic Cardiac Progenitor Cells
Macrophage
Myocardial Infarction
Rat
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The adult heart contains a population of cardiac progenitor cells (CPCs). Growing and collecting an adequate number of CPCs demands complex culture media containing growth factors. Since activated macrophages secrete many growth factors, we investigated if activated isolated heart cells seeded on a feeder layer of activated peritoneal macrophages (PM) could result in CPCs and if these, in turn, could exert cardioprotection in rats with myocardial infarction (MI). Heart cells of inbred Wistar rats were isolated by collagenase digestion and cultured on PM obtained 72 h after intraperitoneal injection of 12 ml thioglycollate. Cells (1 × 106) exhibiting CPC phenotype (immunohistochemistry) were injected in the periphery of rat MI 10 min after coronary artery occlusion. Control rats received vehicle. Three weeks later, left ventricular (LV) function (echocardiogram) was assessed, animals were euthanized and the hearts removed for histological studies. Five to six days after seeding heart cells on PM, spherical clusters composed of small bright and spherical cells expressing mostly c-Kit and Sca-1 antigens were apparent. After explant, those clusters developed cobblestone-like monolayers that expressed smooth muscle actin and sarcomeric actin and were successfully transferred for more than ten passages. When injected in the MI periphery, many of them survived at 21 days after coronary ligature, improved LV ejection fraction and decreased scar size as compared with control rats. CPC-derived cells with cardiocyte and smooth muscle phenotypes can be successfully grown on a feeder layer of activated syngeneic PM. These cells decreased scar size and improved heart function in rats with MI.
Fil: Sepúlveda, Diana Elizabeth. Universidad Favaloro; Argentina
Fil: Cabeza Meckert, Patricia. Universidad Favaloro; Argentina
Fil: Locatelli, Paola. Universidad Favaloro; Argentina
Fil: Olea, Fernanda Daniela. Universidad Favaloro; Argentina
Fil: Perez, Nestor Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Fil: Pinilla, Oscar Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Fil: Diaz, Romina Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Cardiovasculares "Dr. Horacio Eugenio Cingolani"; Argentina
Fil: Crottogini, Alberto José. Universidad Favaloro; Argentina
Fil: Laguens, Rubén. Universidad Favaloro; Argentina
description The adult heart contains a population of cardiac progenitor cells (CPCs). Growing and collecting an adequate number of CPCs demands complex culture media containing growth factors. Since activated macrophages secrete many growth factors, we investigated if activated isolated heart cells seeded on a feeder layer of activated peritoneal macrophages (PM) could result in CPCs and if these, in turn, could exert cardioprotection in rats with myocardial infarction (MI). Heart cells of inbred Wistar rats were isolated by collagenase digestion and cultured on PM obtained 72 h after intraperitoneal injection of 12 ml thioglycollate. Cells (1 × 106) exhibiting CPC phenotype (immunohistochemistry) were injected in the periphery of rat MI 10 min after coronary artery occlusion. Control rats received vehicle. Three weeks later, left ventricular (LV) function (echocardiogram) was assessed, animals were euthanized and the hearts removed for histological studies. Five to six days after seeding heart cells on PM, spherical clusters composed of small bright and spherical cells expressing mostly c-Kit and Sca-1 antigens were apparent. After explant, those clusters developed cobblestone-like monolayers that expressed smooth muscle actin and sarcomeric actin and were successfully transferred for more than ten passages. When injected in the MI periphery, many of them survived at 21 days after coronary ligature, improved LV ejection fraction and decreased scar size as compared with control rats. CPC-derived cells with cardiocyte and smooth muscle phenotypes can be successfully grown on a feeder layer of activated syngeneic PM. These cells decreased scar size and improved heart function in rats with MI.
publishDate 2014
dc.date.none.fl_str_mv 2014-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36443
Sepúlveda, Diana Elizabeth; Cabeza Meckert, Patricia; Locatelli, Paola; Olea, Fernanda Daniela; Perez, Nestor Gustavo; et al.; Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells; Springer; Cytotechnology (dordrecht); 68; 4; 11-2014; 665-674
0920-9069
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36443
identifier_str_mv Sepúlveda, Diana Elizabeth; Cabeza Meckert, Patricia; Locatelli, Paola; Olea, Fernanda Daniela; Perez, Nestor Gustavo; et al.; Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells; Springer; Cytotechnology (dordrecht); 68; 4; 11-2014; 665-674
0920-9069
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10616-014-9814-0
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10616-014-9814-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.018236

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