Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells

Autores
Sepúlveda, Diana Elizabeth; Cabeza Meckert, Patricia Mildred; Locatelli, Paola; Olea, Fernanda Daniela; Pérez, Néstor Gustavo; Pinilla, Oscar Andrés; Díaz, Romina Gisel; Crottogini, Alberto José; Laguens, Rubén P.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The adult heart contains a population of cardiac progenitor cells (CPCs). Growing and collecting an adequate number of CPCs demands complex culture media containing growth factors. Since activated macrophages secrete many growth factors, we investigated if activated isolated heart cells seeded on a feeder layer of activated peritoneal macrophages (PM) could result in CPCs and if these, in turn, could exert cardioprotection in rats with myocardial infarction (MI). Heart cells of inbred Wistar rats were isolated by collagenase digestion and cultured on PM obtained 72 h after intraperitoneal injection of 12 ml thioglycollate. Cells (1 × 106) exhibiting CPC phenotype (immunohistochemistry) were injected in the periphery of rat MI 10 min after coronary artery occlusion. Control rats received vehicle. Three weeks later, left ventricular (LV) function (echocardiogram) was assessed, animals were euthanized and the hearts removed for histological studies. Five to six days after seeding heart cells on PM, spherical clusters composed of small bright and spherical cells expressing mostly c-Kit and Sca-1 antigens were apparent. After explant, those clusters developed cobblestone-like monolayers that expressed smooth muscle actin and sarcomeric actin and were successfully transferred for more than ten passages. When injected in the MI periphery, many of them survived at 21 days after coronary ligature, improved LV ejection fraction and decreased scar size as compared with control rats. CPC-derived cells with cardiocyte and smooth muscle phenotypes can be successfully grown on a feeder layer of activated syngeneic PM. These cells decreased scar size and improved heart function in rats with MI.
Facultad de Ciencias Médicas
Centro de Investigaciones Cardiovasculares
Materia
Ciencias Médicas
Cardiac progenitor cells
Macrophage
Myocardial infarction
Rat
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/101548
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/101548
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Activated macrophages as a feeder layer for growth of resident cardiac progenitor cellsSepúlveda, Diana ElizabethCabeza Meckert, Patricia MildredLocatelli, PaolaOlea, Fernanda DanielaPérez, Néstor GustavoPinilla, Oscar AndrésDíaz, Romina GiselCrottogini, Alberto JoséLaguens, Rubén P.Ciencias MédicasCardiac progenitor cellsMacrophageMyocardial infarctionRatThe adult heart contains a population of cardiac progenitor cells (CPCs). Growing and collecting an adequate number of CPCs demands complex culture media containing growth factors. Since activated macrophages secrete many growth factors, we investigated if activated isolated heart cells seeded on a feeder layer of activated peritoneal macrophages (PM) could result in CPCs and if these, in turn, could exert cardioprotection in rats with myocardial infarction (MI). Heart cells of inbred Wistar rats were isolated by collagenase digestion and cultured on PM obtained 72 h after intraperitoneal injection of 12 ml thioglycollate. Cells (1 × 106) exhibiting CPC phenotype (immunohistochemistry) were injected in the periphery of rat MI 10 min after coronary artery occlusion. Control rats received vehicle. Three weeks later, left ventricular (LV) function (echocardiogram) was assessed, animals were euthanized and the hearts removed for histological studies. Five to six days after seeding heart cells on PM, spherical clusters composed of small bright and spherical cells expressing mostly c-Kit and Sca-1 antigens were apparent. After explant, those clusters developed cobblestone-like monolayers that expressed smooth muscle actin and sarcomeric actin and were successfully transferred for more than ten passages. When injected in the MI periphery, many of them survived at 21 days after coronary ligature, improved LV ejection fraction and decreased scar size as compared with control rats. CPC-derived cells with cardiocyte and smooth muscle phenotypes can be successfully grown on a feeder layer of activated syngeneic PM. These cells decreased scar size and improved heart function in rats with MI.Facultad de Ciencias MédicasCentro de Investigaciones Cardiovasculares2014-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf665-674http://sedici.unlp.edu.ar/handle/10915/101548enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/36443info:eu-repo/semantics/altIdentifier/issn/0920-9069info:eu-repo/semantics/altIdentifier/doi/10.1007/s10616-014-9814-0info:eu-repo/semantics/altIdentifier/hdl/11336/36443info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2026-04-08T10:10:21Zoai:sedici.unlp.edu.ar:10915/101548Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292026-04-08 10:10:22.226SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells
title Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells
spellingShingle Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells
Sepúlveda, Diana Elizabeth
Ciencias Médicas
Cardiac progenitor cells
Macrophage
Myocardial infarction
Rat
title_short Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells
title_full Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells
title_fullStr Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells
title_full_unstemmed Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells
title_sort Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells
dc.creator.none.fl_str_mv Sepúlveda, Diana Elizabeth
Cabeza Meckert, Patricia Mildred
Locatelli, Paola
Olea, Fernanda Daniela
Pérez, Néstor Gustavo
Pinilla, Oscar Andrés
Díaz, Romina Gisel
Crottogini, Alberto José
Laguens, Rubén P.
author Sepúlveda, Diana Elizabeth
author_facet Sepúlveda, Diana Elizabeth
Cabeza Meckert, Patricia Mildred
Locatelli, Paola
Olea, Fernanda Daniela
Pérez, Néstor Gustavo
Pinilla, Oscar Andrés
Díaz, Romina Gisel
Crottogini, Alberto José
Laguens, Rubén P.
author_role author
author2 Cabeza Meckert, Patricia Mildred
Locatelli, Paola
Olea, Fernanda Daniela
Pérez, Néstor Gustavo
Pinilla, Oscar Andrés
Díaz, Romina Gisel
Crottogini, Alberto José
Laguens, Rubén P.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
Cardiac progenitor cells
Macrophage
Myocardial infarction
Rat
topic Ciencias Médicas
Cardiac progenitor cells
Macrophage
Myocardial infarction
Rat
dc.description.none.fl_txt_mv The adult heart contains a population of cardiac progenitor cells (CPCs). Growing and collecting an adequate number of CPCs demands complex culture media containing growth factors. Since activated macrophages secrete many growth factors, we investigated if activated isolated heart cells seeded on a feeder layer of activated peritoneal macrophages (PM) could result in CPCs and if these, in turn, could exert cardioprotection in rats with myocardial infarction (MI). Heart cells of inbred Wistar rats were isolated by collagenase digestion and cultured on PM obtained 72 h after intraperitoneal injection of 12 ml thioglycollate. Cells (1 × 106) exhibiting CPC phenotype (immunohistochemistry) were injected in the periphery of rat MI 10 min after coronary artery occlusion. Control rats received vehicle. Three weeks later, left ventricular (LV) function (echocardiogram) was assessed, animals were euthanized and the hearts removed for histological studies. Five to six days after seeding heart cells on PM, spherical clusters composed of small bright and spherical cells expressing mostly c-Kit and Sca-1 antigens were apparent. After explant, those clusters developed cobblestone-like monolayers that expressed smooth muscle actin and sarcomeric actin and were successfully transferred for more than ten passages. When injected in the MI periphery, many of them survived at 21 days after coronary ligature, improved LV ejection fraction and decreased scar size as compared with control rats. CPC-derived cells with cardiocyte and smooth muscle phenotypes can be successfully grown on a feeder layer of activated syngeneic PM. These cells decreased scar size and improved heart function in rats with MI.
Facultad de Ciencias Médicas
Centro de Investigaciones Cardiovasculares
description The adult heart contains a population of cardiac progenitor cells (CPCs). Growing and collecting an adequate number of CPCs demands complex culture media containing growth factors. Since activated macrophages secrete many growth factors, we investigated if activated isolated heart cells seeded on a feeder layer of activated peritoneal macrophages (PM) could result in CPCs and if these, in turn, could exert cardioprotection in rats with myocardial infarction (MI). Heart cells of inbred Wistar rats were isolated by collagenase digestion and cultured on PM obtained 72 h after intraperitoneal injection of 12 ml thioglycollate. Cells (1 × 106) exhibiting CPC phenotype (immunohistochemistry) were injected in the periphery of rat MI 10 min after coronary artery occlusion. Control rats received vehicle. Three weeks later, left ventricular (LV) function (echocardiogram) was assessed, animals were euthanized and the hearts removed for histological studies. Five to six days after seeding heart cells on PM, spherical clusters composed of small bright and spherical cells expressing mostly c-Kit and Sca-1 antigens were apparent. After explant, those clusters developed cobblestone-like monolayers that expressed smooth muscle actin and sarcomeric actin and were successfully transferred for more than ten passages. When injected in the MI periphery, many of them survived at 21 days after coronary ligature, improved LV ejection fraction and decreased scar size as compared with control rats. CPC-derived cells with cardiocyte and smooth muscle phenotypes can be successfully grown on a feeder layer of activated syngeneic PM. These cells decreased scar size and improved heart function in rats with MI.
publishDate 2014
dc.date.none.fl_str_mv 2014-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/101548
url http://sedici.unlp.edu.ar/handle/10915/101548
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/36443
info:eu-repo/semantics/altIdentifier/issn/0920-9069
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10616-014-9814-0
info:eu-repo/semantics/altIdentifier/hdl/11336/36443
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
665-674
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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