In vitro evaluation of the immunomodulatory and wakame assimilation properties of Lactiplantibacillus plantarum strains from swine milk

Autores
Ohgi, Ryusuke; Saha, Sudeb; Zhou, Binghui; Sakuma, Taiga; Sakurai, Mitsuki; Nakano, Yuhka; Namai, Fu; Ikeda Ohtsubo, Wakako; Suda, Yoshihito; Nishiyama, Keita; Villena, Julio Cesar; Kitazawa, Haruki
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The emergence and spread of antibiotic resistance threat forced to explore alternative strategies for improving the resistance to pathogens in livestock production. Probiotic lactic acid bacteria represent an alternative for this objective. In this study, seven Lactiplantibacillus plantarum strains from porcine colostrum and milk were isolated, identified and characterized in terms of their abilities to modulate immunity in porcine intestinal epithelial (PIE) cells. Then, two potential immunoregulatory strains were studied in terms of their ability to utilize and grow in wakame (Undaria pinnafida). Isolates were identified by 16S rRNA gene and evaluated by studying their interaction with PIE cells. The expressions of peptidoglycan recognition proteins (PGRPs), nucleotide-binding oligomerization domain (NODs), host defense peptides (pBD), and type I interferons (IFNs) were evaluated by RT-qPCR. The strain 4M4417 showed a remarkable capacity to differentially regulate the expression of PGRP1, PGRP3, NOD1, NOD2, and pBD1 in PIE cells. On the other hand, the strain 4M4326 was the most efficient to improve the expression of IFN-α and IFN-β in PIE cells challenged with poly (I:C). Both L. plantarum 4M4326 and 4M4417 were characterized in terms of their ability to utilize wakame. Results demonstrated that both strains efficiently grew in wakame-based broth. Our results suggest that L. planatrum 4M4326 and 4M4417 are interesting candidates to develop immunomodulatory feeds based on wakame utilization. These new immunosynbiotic feeds could help to reduce severity of intestinal infections and improve immune health status in pigs.
Fil: Ohgi, Ryusuke. Tohoku University; Japón
Fil: Saha, Sudeb. Tohoku University; Japón. Sylhet Agricultural University; Bangladesh
Fil: Zhou, Binghui. Tohoku University; Japón
Fil: Sakuma, Taiga. Tohoku University; Japón
Fil: Sakurai, Mitsuki. Tohoku University; Japón
Fil: Nakano, Yuhka. Tohoku University; Japón
Fil: Namai, Fu. Tohoku University; Japón
Fil: Ikeda Ohtsubo, Wakako. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Nishiyama, Keita. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Kitazawa, Haruki. Tohoku University; Japón
Materia
PORCINE INTESTINAL EPITHELIAL CELLS
PIG MILK
LACTOBACILLUS PLANTARUM
IMMUNOMODULATORY POTENTIAL
WAKAME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/260641
Acceder
id CONICETDig_8159a865f7184fe62c353b58ed3968b4
oai_identifier_str oai:ri.conicet.gov.ar:11336/260641
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro evaluation of the immunomodulatory and wakame assimilation properties of Lactiplantibacillus plantarum strains from swine milkOhgi, RyusukeSaha, SudebZhou, BinghuiSakuma, TaigaSakurai, MitsukiNakano, YuhkaNamai, FuIkeda Ohtsubo, WakakoSuda, YoshihitoNishiyama, KeitaVillena, Julio CesarKitazawa, HarukiPORCINE INTESTINAL EPITHELIAL CELLSPIG MILKLACTOBACILLUS PLANTARUMIMMUNOMODULATORY POTENTIALWAKAMEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The emergence and spread of antibiotic resistance threat forced to explore alternative strategies for improving the resistance to pathogens in livestock production. Probiotic lactic acid bacteria represent an alternative for this objective. In this study, seven Lactiplantibacillus plantarum strains from porcine colostrum and milk were isolated, identified and characterized in terms of their abilities to modulate immunity in porcine intestinal epithelial (PIE) cells. Then, two potential immunoregulatory strains were studied in terms of their ability to utilize and grow in wakame (Undaria pinnafida). Isolates were identified by 16S rRNA gene and evaluated by studying their interaction with PIE cells. The expressions of peptidoglycan recognition proteins (PGRPs), nucleotide-binding oligomerization domain (NODs), host defense peptides (pBD), and type I interferons (IFNs) were evaluated by RT-qPCR. The strain 4M4417 showed a remarkable capacity to differentially regulate the expression of PGRP1, PGRP3, NOD1, NOD2, and pBD1 in PIE cells. On the other hand, the strain 4M4326 was the most efficient to improve the expression of IFN-α and IFN-β in PIE cells challenged with poly (I:C). Both L. plantarum 4M4326 and 4M4417 were characterized in terms of their ability to utilize wakame. Results demonstrated that both strains efficiently grew in wakame-based broth. Our results suggest that L. planatrum 4M4326 and 4M4417 are interesting candidates to develop immunomodulatory feeds based on wakame utilization. These new immunosynbiotic feeds could help to reduce severity of intestinal infections and improve immune health status in pigs.Fil: Ohgi, Ryusuke. Tohoku University; JapónFil: Saha, Sudeb. Tohoku University; Japón. Sylhet Agricultural University; BangladeshFil: Zhou, Binghui. Tohoku University; JapónFil: Sakuma, Taiga. Tohoku University; JapónFil: Sakurai, Mitsuki. Tohoku University; JapónFil: Nakano, Yuhka. Tohoku University; JapónFil: Namai, Fu. Tohoku University; JapónFil: Ikeda Ohtsubo, Wakako. Tohoku University; JapónFil: Suda, Yoshihito. Miyagi University; JapónFil: Nishiyama, Keita. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Kitazawa, Haruki. Tohoku University; JapónFrontiers Media2024-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/260641Ohgi, Ryusuke; Saha, Sudeb; Zhou, Binghui; Sakuma, Taiga; Sakurai, Mitsuki; et al.; In vitro evaluation of the immunomodulatory and wakame assimilation properties of Lactiplantibacillus plantarum strains from swine milk; Frontiers Media; Frontiers in Microbiology; 15; 1324999; 1-2024; 1-101664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2024.1324999/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2024.1324999info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:24:26Zoai:ri.conicet.gov.ar:11336/260641instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:24:26.33CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro evaluation of the immunomodulatory and wakame assimilation properties of Lactiplantibacillus plantarum strains from swine milk
title In vitro evaluation of the immunomodulatory and wakame assimilation properties of Lactiplantibacillus plantarum strains from swine milk
spellingShingle In vitro evaluation of the immunomodulatory and wakame assimilation properties of Lactiplantibacillus plantarum strains from swine milk
Ohgi, Ryusuke
PORCINE INTESTINAL EPITHELIAL CELLS
PIG MILK
LACTOBACILLUS PLANTARUM
IMMUNOMODULATORY POTENTIAL
WAKAME
title_short In vitro evaluation of the immunomodulatory and wakame assimilation properties of Lactiplantibacillus plantarum strains from swine milk
title_full In vitro evaluation of the immunomodulatory and wakame assimilation properties of Lactiplantibacillus plantarum strains from swine milk
title_fullStr In vitro evaluation of the immunomodulatory and wakame assimilation properties of Lactiplantibacillus plantarum strains from swine milk
title_full_unstemmed In vitro evaluation of the immunomodulatory and wakame assimilation properties of Lactiplantibacillus plantarum strains from swine milk
title_sort In vitro evaluation of the immunomodulatory and wakame assimilation properties of Lactiplantibacillus plantarum strains from swine milk
dc.creator.none.fl_str_mv Ohgi, Ryusuke
Saha, Sudeb
Zhou, Binghui
Sakuma, Taiga
Sakurai, Mitsuki
Nakano, Yuhka
Namai, Fu
Ikeda Ohtsubo, Wakako
Suda, Yoshihito
Nishiyama, Keita
Villena, Julio Cesar
Kitazawa, Haruki
author Ohgi, Ryusuke
author_facet Ohgi, Ryusuke
Saha, Sudeb
Zhou, Binghui
Sakuma, Taiga
Sakurai, Mitsuki
Nakano, Yuhka
Namai, Fu
Ikeda Ohtsubo, Wakako
Suda, Yoshihito
Nishiyama, Keita
Villena, Julio Cesar
Kitazawa, Haruki
author_role author
author2 Saha, Sudeb
Zhou, Binghui
Sakuma, Taiga
Sakurai, Mitsuki
Nakano, Yuhka
Namai, Fu
Ikeda Ohtsubo, Wakako
Suda, Yoshihito
Nishiyama, Keita
Villena, Julio Cesar
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PORCINE INTESTINAL EPITHELIAL CELLS
PIG MILK
LACTOBACILLUS PLANTARUM
IMMUNOMODULATORY POTENTIAL
WAKAME
topic PORCINE INTESTINAL EPITHELIAL CELLS
PIG MILK
LACTOBACILLUS PLANTARUM
IMMUNOMODULATORY POTENTIAL
WAKAME
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The emergence and spread of antibiotic resistance threat forced to explore alternative strategies for improving the resistance to pathogens in livestock production. Probiotic lactic acid bacteria represent an alternative for this objective. In this study, seven Lactiplantibacillus plantarum strains from porcine colostrum and milk were isolated, identified and characterized in terms of their abilities to modulate immunity in porcine intestinal epithelial (PIE) cells. Then, two potential immunoregulatory strains were studied in terms of their ability to utilize and grow in wakame (Undaria pinnafida). Isolates were identified by 16S rRNA gene and evaluated by studying their interaction with PIE cells. The expressions of peptidoglycan recognition proteins (PGRPs), nucleotide-binding oligomerization domain (NODs), host defense peptides (pBD), and type I interferons (IFNs) were evaluated by RT-qPCR. The strain 4M4417 showed a remarkable capacity to differentially regulate the expression of PGRP1, PGRP3, NOD1, NOD2, and pBD1 in PIE cells. On the other hand, the strain 4M4326 was the most efficient to improve the expression of IFN-α and IFN-β in PIE cells challenged with poly (I:C). Both L. plantarum 4M4326 and 4M4417 were characterized in terms of their ability to utilize wakame. Results demonstrated that both strains efficiently grew in wakame-based broth. Our results suggest that L. planatrum 4M4326 and 4M4417 are interesting candidates to develop immunomodulatory feeds based on wakame utilization. These new immunosynbiotic feeds could help to reduce severity of intestinal infections and improve immune health status in pigs.
Fil: Ohgi, Ryusuke. Tohoku University; Japón
Fil: Saha, Sudeb. Tohoku University; Japón. Sylhet Agricultural University; Bangladesh
Fil: Zhou, Binghui. Tohoku University; Japón
Fil: Sakuma, Taiga. Tohoku University; Japón
Fil: Sakurai, Mitsuki. Tohoku University; Japón
Fil: Nakano, Yuhka. Tohoku University; Japón
Fil: Namai, Fu. Tohoku University; Japón
Fil: Ikeda Ohtsubo, Wakako. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Nishiyama, Keita. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Kitazawa, Haruki. Tohoku University; Japón
description The emergence and spread of antibiotic resistance threat forced to explore alternative strategies for improving the resistance to pathogens in livestock production. Probiotic lactic acid bacteria represent an alternative for this objective. In this study, seven Lactiplantibacillus plantarum strains from porcine colostrum and milk were isolated, identified and characterized in terms of their abilities to modulate immunity in porcine intestinal epithelial (PIE) cells. Then, two potential immunoregulatory strains were studied in terms of their ability to utilize and grow in wakame (Undaria pinnafida). Isolates were identified by 16S rRNA gene and evaluated by studying their interaction with PIE cells. The expressions of peptidoglycan recognition proteins (PGRPs), nucleotide-binding oligomerization domain (NODs), host defense peptides (pBD), and type I interferons (IFNs) were evaluated by RT-qPCR. The strain 4M4417 showed a remarkable capacity to differentially regulate the expression of PGRP1, PGRP3, NOD1, NOD2, and pBD1 in PIE cells. On the other hand, the strain 4M4326 was the most efficient to improve the expression of IFN-α and IFN-β in PIE cells challenged with poly (I:C). Both L. plantarum 4M4326 and 4M4417 were characterized in terms of their ability to utilize wakame. Results demonstrated that both strains efficiently grew in wakame-based broth. Our results suggest that L. planatrum 4M4326 and 4M4417 are interesting candidates to develop immunomodulatory feeds based on wakame utilization. These new immunosynbiotic feeds could help to reduce severity of intestinal infections and improve immune health status in pigs.
publishDate 2024
dc.date.none.fl_str_mv 2024-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/260641
Ohgi, Ryusuke; Saha, Sudeb; Zhou, Binghui; Sakuma, Taiga; Sakurai, Mitsuki; et al.; In vitro evaluation of the immunomodulatory and wakame assimilation properties of Lactiplantibacillus plantarum strains from swine milk; Frontiers Media; Frontiers in Microbiology; 15; 1324999; 1-2024; 1-10
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/260641
identifier_str_mv Ohgi, Ryusuke; Saha, Sudeb; Zhou, Binghui; Sakuma, Taiga; Sakurai, Mitsuki; et al.; In vitro evaluation of the immunomodulatory and wakame assimilation properties of Lactiplantibacillus plantarum strains from swine milk; Frontiers Media; Frontiers in Microbiology; 15; 1324999; 1-2024; 1-10
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2024.1324999/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2024.1324999
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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