Selection of immunobiotic ligilactobacillus salivarius strains from the intestinal tract of wakame-fed pigs: Functional and genomic studies
- Autores
- Zhou, Binghui; Albarracín, Leonardo Miguel; Indo, Yuhki; Arce, Lorena Paola; Masumizu, Yuki; Tomokiyo, Mikado; Islam, Md Aminul; Garcia Castillo, Valeria; Ikeda Ohtsubo, Wakako; Nochi, Tomonori; Morita, Hidetoshi; Takahashi, Hideki; Kurata, Shoichiro; Villena, Julio Cesar; Kitazawa, Haruki
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In this article, Ligilactobacillus salivarius FFIG strains, isolated from the intestinal tract of wakame-fed pigs, are characterized according to their potential probiotic properties. Strains were evaluated by studying their interaction with porcine intestinal epithelial (PIE) cells in terms of their ability to regulate toll-like receptor (TLR)-3- or TLR4-mediated innate immune responses, as well as by assessing their adhesion capabilities to porcine epithelial cells and mucins. These functional studies were complemented with comparative genomic evaluations using the complete genome sequences of porcine L. salivarius strains selected from subgroups that demonstrated different ‘immune’ and ‘adhesion’ phenotypes. We found that their immunomodulatory and adhesion capabilities are a strain-dependent characteristic. Our analysis indicated that the differential immunomodulatory and adhesive activities of FFIG strains would be dependent on the combination of several surface structures acting simultaneously, which include peptidoglycan, exopolysaccharides, lipoteichoic acid, and adhesins. Of note, our results indicate that there is no correlation between the immunomodulatory capacity of the strains with their adhesion ability to mucins and epithelial cells. Therefore, in the selection of strains destined to colonize the intestinal mucosa and modulate the immunity of the host, both properties must be adequately evaluated. Interestingly, we showed that L. salivarius FFIG58 functionally modulated the innate immune responses triggered by TLR3 and TLR4 activation in PIE cells and efficiently adhered to these cells. Moreover, the FFIG58 strain was capable of reducing rotavirus replication in PIE cells. Therefore, L. salivarius FFIG58 is a good candidate for further in vivo studying the protective effect of lactobacilli against intestinal infections in the porcine host. We also reported and analyzed, for the first time, the complete genome of several L. salivarius strains that were isolated from the intestine of pigs after the selective pressure of feeding the animals with wakame. Further genomic analysis could be of value to reveal the metabolic characteristics and potential of the FFIG strains in general and of the FFIG58 strain, in particular, relating to wakame by-products assimilation.
Fil: Zhou, Binghui. Tohoku University; Japón
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Ciencias de la Computación; Argentina. Tohoku University; Japón
Fil: Indo, Yuhki. Tohoku University; Japón
Fil: Arce, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Tohoku University; Japón
Fil: Masumizu, Yuki. Tohoku University; Japón
Fil: Tomokiyo, Mikado. Tohoku University; Japón
Fil: Islam, Md Aminul. Bangladesh Agricultural University; Bangladesh. Tohoku University; Japón
Fil: Garcia Castillo, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Ikeda Ohtsubo, Wakako. Tohoku University; Japón
Fil: Nochi, Tomonori. Tohoku University; Japón
Fil: Morita, Hidetoshi. Okayama University; Japón
Fil: Takahashi, Hideki. Tohoku University; Japón
Fil: Kurata, Shoichiro. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón - Materia
-
ADHESION
GENOMICS
IMMUNOMODULATION
LIGILACTOBACILLUS SALIVARIUS
PIGS
PORCINE INTESTINAL EPITHELIAL CELLS
WAKAME - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/130333
Ver los metadatos del registro completo
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Selection of immunobiotic ligilactobacillus salivarius strains from the intestinal tract of wakame-fed pigs: Functional and genomic studiesZhou, BinghuiAlbarracín, Leonardo MiguelIndo, YuhkiArce, Lorena PaolaMasumizu, YukiTomokiyo, MikadoIslam, Md AminulGarcia Castillo, ValeriaIkeda Ohtsubo, WakakoNochi, TomonoriMorita, HidetoshiTakahashi, HidekiKurata, ShoichiroVillena, Julio CesarKitazawa, HarukiADHESIONGENOMICSIMMUNOMODULATIONLIGILACTOBACILLUS SALIVARIUSPIGSPORCINE INTESTINAL EPITHELIAL CELLSWAKAMEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In this article, Ligilactobacillus salivarius FFIG strains, isolated from the intestinal tract of wakame-fed pigs, are characterized according to their potential probiotic properties. Strains were evaluated by studying their interaction with porcine intestinal epithelial (PIE) cells in terms of their ability to regulate toll-like receptor (TLR)-3- or TLR4-mediated innate immune responses, as well as by assessing their adhesion capabilities to porcine epithelial cells and mucins. These functional studies were complemented with comparative genomic evaluations using the complete genome sequences of porcine L. salivarius strains selected from subgroups that demonstrated different ‘immune’ and ‘adhesion’ phenotypes. We found that their immunomodulatory and adhesion capabilities are a strain-dependent characteristic. Our analysis indicated that the differential immunomodulatory and adhesive activities of FFIG strains would be dependent on the combination of several surface structures acting simultaneously, which include peptidoglycan, exopolysaccharides, lipoteichoic acid, and adhesins. Of note, our results indicate that there is no correlation between the immunomodulatory capacity of the strains with their adhesion ability to mucins and epithelial cells. Therefore, in the selection of strains destined to colonize the intestinal mucosa and modulate the immunity of the host, both properties must be adequately evaluated. Interestingly, we showed that L. salivarius FFIG58 functionally modulated the innate immune responses triggered by TLR3 and TLR4 activation in PIE cells and efficiently adhered to these cells. Moreover, the FFIG58 strain was capable of reducing rotavirus replication in PIE cells. Therefore, L. salivarius FFIG58 is a good candidate for further in vivo studying the protective effect of lactobacilli against intestinal infections in the porcine host. We also reported and analyzed, for the first time, the complete genome of several L. salivarius strains that were isolated from the intestine of pigs after the selective pressure of feeding the animals with wakame. Further genomic analysis could be of value to reveal the metabolic characteristics and potential of the FFIG strains in general and of the FFIG58 strain, in particular, relating to wakame by-products assimilation.Fil: Zhou, Binghui. Tohoku University; JapónFil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Ciencias de la Computación; Argentina. Tohoku University; JapónFil: Indo, Yuhki. Tohoku University; JapónFil: Arce, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Tohoku University; JapónFil: Masumizu, Yuki. Tohoku University; JapónFil: Tomokiyo, Mikado. Tohoku University; JapónFil: Islam, Md Aminul. Bangladesh Agricultural University; Bangladesh. Tohoku University; JapónFil: Garcia Castillo, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; JapónFil: Ikeda Ohtsubo, Wakako. Tohoku University; JapónFil: Nochi, Tomonori. Tohoku University; JapónFil: Morita, Hidetoshi. Okayama University; JapónFil: Takahashi, Hideki. Tohoku University; JapónFil: Kurata, Shoichiro. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; JapónFil: Kitazawa, Haruki. Tohoku University; JapónMDPI AG2020-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/130333Zhou, Binghui; Albarracín, Leonardo Miguel; Indo, Yuhki; Arce, Lorena Paola; Masumizu, Yuki; et al.; Selection of immunobiotic ligilactobacillus salivarius strains from the intestinal tract of wakame-fed pigs: Functional and genomic studies; MDPI AG; Microorganisms; 8; 11; 11-2020; 1-28;16592076-26072076-2607CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms8111659info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/8/11/1659info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:34Zoai:ri.conicet.gov.ar:11336/130333instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:35.133CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Selection of immunobiotic ligilactobacillus salivarius strains from the intestinal tract of wakame-fed pigs: Functional and genomic studies |
| title |
Selection of immunobiotic ligilactobacillus salivarius strains from the intestinal tract of wakame-fed pigs: Functional and genomic studies |
| spellingShingle |
Selection of immunobiotic ligilactobacillus salivarius strains from the intestinal tract of wakame-fed pigs: Functional and genomic studies Zhou, Binghui ADHESION GENOMICS IMMUNOMODULATION LIGILACTOBACILLUS SALIVARIUS PIGS PORCINE INTESTINAL EPITHELIAL CELLS WAKAME |
| title_short |
Selection of immunobiotic ligilactobacillus salivarius strains from the intestinal tract of wakame-fed pigs: Functional and genomic studies |
| title_full |
Selection of immunobiotic ligilactobacillus salivarius strains from the intestinal tract of wakame-fed pigs: Functional and genomic studies |
| title_fullStr |
Selection of immunobiotic ligilactobacillus salivarius strains from the intestinal tract of wakame-fed pigs: Functional and genomic studies |
| title_full_unstemmed |
Selection of immunobiotic ligilactobacillus salivarius strains from the intestinal tract of wakame-fed pigs: Functional and genomic studies |
| title_sort |
Selection of immunobiotic ligilactobacillus salivarius strains from the intestinal tract of wakame-fed pigs: Functional and genomic studies |
| dc.creator.none.fl_str_mv |
Zhou, Binghui Albarracín, Leonardo Miguel Indo, Yuhki Arce, Lorena Paola Masumizu, Yuki Tomokiyo, Mikado Islam, Md Aminul Garcia Castillo, Valeria Ikeda Ohtsubo, Wakako Nochi, Tomonori Morita, Hidetoshi Takahashi, Hideki Kurata, Shoichiro Villena, Julio Cesar Kitazawa, Haruki |
| author |
Zhou, Binghui |
| author_facet |
Zhou, Binghui Albarracín, Leonardo Miguel Indo, Yuhki Arce, Lorena Paola Masumizu, Yuki Tomokiyo, Mikado Islam, Md Aminul Garcia Castillo, Valeria Ikeda Ohtsubo, Wakako Nochi, Tomonori Morita, Hidetoshi Takahashi, Hideki Kurata, Shoichiro Villena, Julio Cesar Kitazawa, Haruki |
| author_role |
author |
| author2 |
Albarracín, Leonardo Miguel Indo, Yuhki Arce, Lorena Paola Masumizu, Yuki Tomokiyo, Mikado Islam, Md Aminul Garcia Castillo, Valeria Ikeda Ohtsubo, Wakako Nochi, Tomonori Morita, Hidetoshi Takahashi, Hideki Kurata, Shoichiro Villena, Julio Cesar Kitazawa, Haruki |
| author2_role |
author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
ADHESION GENOMICS IMMUNOMODULATION LIGILACTOBACILLUS SALIVARIUS PIGS PORCINE INTESTINAL EPITHELIAL CELLS WAKAME |
| topic |
ADHESION GENOMICS IMMUNOMODULATION LIGILACTOBACILLUS SALIVARIUS PIGS PORCINE INTESTINAL EPITHELIAL CELLS WAKAME |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| dc.description.none.fl_txt_mv |
In this article, Ligilactobacillus salivarius FFIG strains, isolated from the intestinal tract of wakame-fed pigs, are characterized according to their potential probiotic properties. Strains were evaluated by studying their interaction with porcine intestinal epithelial (PIE) cells in terms of their ability to regulate toll-like receptor (TLR)-3- or TLR4-mediated innate immune responses, as well as by assessing their adhesion capabilities to porcine epithelial cells and mucins. These functional studies were complemented with comparative genomic evaluations using the complete genome sequences of porcine L. salivarius strains selected from subgroups that demonstrated different ‘immune’ and ‘adhesion’ phenotypes. We found that their immunomodulatory and adhesion capabilities are a strain-dependent characteristic. Our analysis indicated that the differential immunomodulatory and adhesive activities of FFIG strains would be dependent on the combination of several surface structures acting simultaneously, which include peptidoglycan, exopolysaccharides, lipoteichoic acid, and adhesins. Of note, our results indicate that there is no correlation between the immunomodulatory capacity of the strains with their adhesion ability to mucins and epithelial cells. Therefore, in the selection of strains destined to colonize the intestinal mucosa and modulate the immunity of the host, both properties must be adequately evaluated. Interestingly, we showed that L. salivarius FFIG58 functionally modulated the innate immune responses triggered by TLR3 and TLR4 activation in PIE cells and efficiently adhered to these cells. Moreover, the FFIG58 strain was capable of reducing rotavirus replication in PIE cells. Therefore, L. salivarius FFIG58 is a good candidate for further in vivo studying the protective effect of lactobacilli against intestinal infections in the porcine host. We also reported and analyzed, for the first time, the complete genome of several L. salivarius strains that were isolated from the intestine of pigs after the selective pressure of feeding the animals with wakame. Further genomic analysis could be of value to reveal the metabolic characteristics and potential of the FFIG strains in general and of the FFIG58 strain, in particular, relating to wakame by-products assimilation. Fil: Zhou, Binghui. Tohoku University; Japón Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Ciencias de la Computación; Argentina. Tohoku University; Japón Fil: Indo, Yuhki. Tohoku University; Japón Fil: Arce, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Tohoku University; Japón Fil: Masumizu, Yuki. Tohoku University; Japón Fil: Tomokiyo, Mikado. Tohoku University; Japón Fil: Islam, Md Aminul. Bangladesh Agricultural University; Bangladesh. Tohoku University; Japón Fil: Garcia Castillo, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón Fil: Ikeda Ohtsubo, Wakako. Tohoku University; Japón Fil: Nochi, Tomonori. Tohoku University; Japón Fil: Morita, Hidetoshi. Okayama University; Japón Fil: Takahashi, Hideki. Tohoku University; Japón Fil: Kurata, Shoichiro. Tohoku University; Japón Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón Fil: Kitazawa, Haruki. Tohoku University; Japón |
| description |
In this article, Ligilactobacillus salivarius FFIG strains, isolated from the intestinal tract of wakame-fed pigs, are characterized according to their potential probiotic properties. Strains were evaluated by studying their interaction with porcine intestinal epithelial (PIE) cells in terms of their ability to regulate toll-like receptor (TLR)-3- or TLR4-mediated innate immune responses, as well as by assessing their adhesion capabilities to porcine epithelial cells and mucins. These functional studies were complemented with comparative genomic evaluations using the complete genome sequences of porcine L. salivarius strains selected from subgroups that demonstrated different ‘immune’ and ‘adhesion’ phenotypes. We found that their immunomodulatory and adhesion capabilities are a strain-dependent characteristic. Our analysis indicated that the differential immunomodulatory and adhesive activities of FFIG strains would be dependent on the combination of several surface structures acting simultaneously, which include peptidoglycan, exopolysaccharides, lipoteichoic acid, and adhesins. Of note, our results indicate that there is no correlation between the immunomodulatory capacity of the strains with their adhesion ability to mucins and epithelial cells. Therefore, in the selection of strains destined to colonize the intestinal mucosa and modulate the immunity of the host, both properties must be adequately evaluated. Interestingly, we showed that L. salivarius FFIG58 functionally modulated the innate immune responses triggered by TLR3 and TLR4 activation in PIE cells and efficiently adhered to these cells. Moreover, the FFIG58 strain was capable of reducing rotavirus replication in PIE cells. Therefore, L. salivarius FFIG58 is a good candidate for further in vivo studying the protective effect of lactobacilli against intestinal infections in the porcine host. We also reported and analyzed, for the first time, the complete genome of several L. salivarius strains that were isolated from the intestine of pigs after the selective pressure of feeding the animals with wakame. Further genomic analysis could be of value to reveal the metabolic characteristics and potential of the FFIG strains in general and of the FFIG58 strain, in particular, relating to wakame by-products assimilation. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-11 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/130333 Zhou, Binghui; Albarracín, Leonardo Miguel; Indo, Yuhki; Arce, Lorena Paola; Masumizu, Yuki; et al.; Selection of immunobiotic ligilactobacillus salivarius strains from the intestinal tract of wakame-fed pigs: Functional and genomic studies; MDPI AG; Microorganisms; 8; 11; 11-2020; 1-28;1659 2076-2607 2076-2607 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/130333 |
| identifier_str_mv |
Zhou, Binghui; Albarracín, Leonardo Miguel; Indo, Yuhki; Arce, Lorena Paola; Masumizu, Yuki; et al.; Selection of immunobiotic ligilactobacillus salivarius strains from the intestinal tract of wakame-fed pigs: Functional and genomic studies; MDPI AG; Microorganisms; 8; 11; 11-2020; 1-28;1659 2076-2607 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms8111659 info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/8/11/1659 |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by/2.5/ar/ |
| dc.format.none.fl_str_mv |
application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
MDPI AG |
| publisher.none.fl_str_mv |
MDPI AG |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613109144092672 |
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13.070432 |