Phenotypic and genotypic evaluation of wakame assimilation ability in Ligilactobacillus salivarius strains isolated from wakame fed pigs

Autores
Elean, Mariano Daniel; Albarracín, Leonardo Miguel; Raya Tonetti, María Fernanda; Ortiz Moyano, Francisco Ramiro; Dentice Maidana, Stefania; Kitazawa, Haruki; Villena, Julio Cesar
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Wakame (Undaria pinnatifida) is a brown alga widely consumed as edible seaweed in Asia. Wakame contains some bioactive components including fucoidan, alginic acid, omega 3 fatty acids, vitamins, and minerals, which has been reported to have beneficial effects in the human host. Among the beneficial effects attributed to wakame are its antibacterial, antioxidant and anti-inflammatory functions. Recently, we demonstrated that feeding pigs with wakame improved gastrointestinal immunity and induced a significant increase in the abundance of members of the Lactobacillus family, specially Ligilactobacillus salivarius. To determine whether the beneficial immunological effects of wakame were associated to the increment of L. salivarius strains, we isolated and characterized strains from wakame-fed pigs belonging to this species. The complete genome of eight selected L. salivarius strains (named FFIG) were sequenced and the functional and genomic characterization of those strains revealed that their immunomodulatory and adhesion capabilities are a strain-specific characteristic. In this work we aimed to further characterize the L. salivarius FFIG strains studying their wakame assimilation abilities by phenotypic and genotypic approaches. A prolonged fermentation experiment was designed to investigate the consumption of the saccharides by the FFIG strains in two different wakame broths (wakame leaf and wakame stalk). TLC analysis showed that the eight FFIG strains were able to utilize the saccharides contained in enzyme-treated wakame. The strains had different preferences for the two wakame broths. In wakame leaf, FFIG58, FFIG24 and FFIG63 were the strains with the highest ability to grow, whereas FFIG130 and FFIG79 were the strains with the lowest bacterial counts. In wakame stalk, the strains FFIG60, FFIG79 and FFIG130 stood out over the eight strains with the highest viable counts, whereas the FFIG58 have the lowest counts. It was reported that L. salivarius strains have different abundance of genes belonging to glycosyltransferases and glycosylhydrolases families, and that the set of enzymes determine the carbon sources that each strain can use for growing. Then, we evaluated the abundance of genes belonging to glycosylhydrolases families among the FFIG strains and compared them with other L. salivarius strains of animal origin. The clustering analysis considering the numbers and types of glycosylhydrolases showed that strains FFIG58, FFIG63, FFIG79 and FFIG124 had a significant higher abundance of enzymes from the families GH25 and GH13, when compared with all the other L. salivarius strains. Finally, we performed comparative genomic studies between the FFIG strains and other strains isolated from human and porcine origins. We found a core genome compound of 710 genes, and there was no correlation between the number of unique genes and the difference in the phenotypes. To the best of our knowledge, this is the first study that evaluated wakame assimilation capacity of L. salivarius strains using phenotypic and genomic approaches.
Fil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Dentice Maidana, Stefania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
7mo Simposio Argentino de Jóvenes Investigadores en Bioinformática
San Miguel de Tucumán
Argentina
Argentine Regional Student Group
Universidad Nacional de Tucumán. Facultad de Bioquímica Química y Farmacia
Materia
LIGILACTOBACILLUS SALIVARIUS
WAKAME ASSIMILATION
PIGS
PROBIOTICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215982

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network_name_str CONICET Digital (CONICET)
spelling Phenotypic and genotypic evaluation of wakame assimilation ability in Ligilactobacillus salivarius strains isolated from wakame fed pigsElean, Mariano DanielAlbarracín, Leonardo MiguelRaya Tonetti, María FernandaOrtiz Moyano, Francisco RamiroDentice Maidana, StefaniaKitazawa, HarukiVillena, Julio CesarLIGILACTOBACILLUS SALIVARIUSWAKAME ASSIMILATIONPIGSPROBIOTICShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Wakame (Undaria pinnatifida) is a brown alga widely consumed as edible seaweed in Asia. Wakame contains some bioactive components including fucoidan, alginic acid, omega 3 fatty acids, vitamins, and minerals, which has been reported to have beneficial effects in the human host. Among the beneficial effects attributed to wakame are its antibacterial, antioxidant and anti-inflammatory functions. Recently, we demonstrated that feeding pigs with wakame improved gastrointestinal immunity and induced a significant increase in the abundance of members of the Lactobacillus family, specially Ligilactobacillus salivarius. To determine whether the beneficial immunological effects of wakame were associated to the increment of L. salivarius strains, we isolated and characterized strains from wakame-fed pigs belonging to this species. The complete genome of eight selected L. salivarius strains (named FFIG) were sequenced and the functional and genomic characterization of those strains revealed that their immunomodulatory and adhesion capabilities are a strain-specific characteristic. In this work we aimed to further characterize the L. salivarius FFIG strains studying their wakame assimilation abilities by phenotypic and genotypic approaches. A prolonged fermentation experiment was designed to investigate the consumption of the saccharides by the FFIG strains in two different wakame broths (wakame leaf and wakame stalk). TLC analysis showed that the eight FFIG strains were able to utilize the saccharides contained in enzyme-treated wakame. The strains had different preferences for the two wakame broths. In wakame leaf, FFIG58, FFIG24 and FFIG63 were the strains with the highest ability to grow, whereas FFIG130 and FFIG79 were the strains with the lowest bacterial counts. In wakame stalk, the strains FFIG60, FFIG79 and FFIG130 stood out over the eight strains with the highest viable counts, whereas the FFIG58 have the lowest counts. It was reported that L. salivarius strains have different abundance of genes belonging to glycosyltransferases and glycosylhydrolases families, and that the set of enzymes determine the carbon sources that each strain can use for growing. Then, we evaluated the abundance of genes belonging to glycosylhydrolases families among the FFIG strains and compared them with other L. salivarius strains of animal origin. The clustering analysis considering the numbers and types of glycosylhydrolases showed that strains FFIG58, FFIG63, FFIG79 and FFIG124 had a significant higher abundance of enzymes from the families GH25 and GH13, when compared with all the other L. salivarius strains. Finally, we performed comparative genomic studies between the FFIG strains and other strains isolated from human and porcine origins. We found a core genome compound of 710 genes, and there was no correlation between the number of unique genes and the difference in the phenotypes. To the best of our knowledge, this is the first study that evaluated wakame assimilation capacity of L. salivarius strains using phenotypic and genomic approaches.Fil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Dentice Maidana, Stefania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaFil: Kitazawa, Haruki. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón7mo Simposio Argentino de Jóvenes Investigadores en BioinformáticaSan Miguel de TucumánArgentinaArgentine Regional Student GroupUniversidad Nacional de Tucumán. Facultad de Bioquímica Química y FarmaciaArgentine Regional Student Group2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectSimposioBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215982Phenotypic and genotypic evaluation of wakame assimilation ability in Ligilactobacillus salivarius strains isolated from wakame fed pigs; 7mo Simposio Argentino de Jóvenes Investigadores en Bioinformática; San Miguel de Tucumán; Argentina; 2022; 25-25CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://zenodo.org/record/7266042info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.5281/zenodo.7266042Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:11:33Zoai:ri.conicet.gov.ar:11336/215982instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:11:33.319CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phenotypic and genotypic evaluation of wakame assimilation ability in Ligilactobacillus salivarius strains isolated from wakame fed pigs
title Phenotypic and genotypic evaluation of wakame assimilation ability in Ligilactobacillus salivarius strains isolated from wakame fed pigs
spellingShingle Phenotypic and genotypic evaluation of wakame assimilation ability in Ligilactobacillus salivarius strains isolated from wakame fed pigs
Elean, Mariano Daniel
LIGILACTOBACILLUS SALIVARIUS
WAKAME ASSIMILATION
PIGS
PROBIOTICS
title_short Phenotypic and genotypic evaluation of wakame assimilation ability in Ligilactobacillus salivarius strains isolated from wakame fed pigs
title_full Phenotypic and genotypic evaluation of wakame assimilation ability in Ligilactobacillus salivarius strains isolated from wakame fed pigs
title_fullStr Phenotypic and genotypic evaluation of wakame assimilation ability in Ligilactobacillus salivarius strains isolated from wakame fed pigs
title_full_unstemmed Phenotypic and genotypic evaluation of wakame assimilation ability in Ligilactobacillus salivarius strains isolated from wakame fed pigs
title_sort Phenotypic and genotypic evaluation of wakame assimilation ability in Ligilactobacillus salivarius strains isolated from wakame fed pigs
dc.creator.none.fl_str_mv Elean, Mariano Daniel
Albarracín, Leonardo Miguel
Raya Tonetti, María Fernanda
Ortiz Moyano, Francisco Ramiro
Dentice Maidana, Stefania
Kitazawa, Haruki
Villena, Julio Cesar
author Elean, Mariano Daniel
author_facet Elean, Mariano Daniel
Albarracín, Leonardo Miguel
Raya Tonetti, María Fernanda
Ortiz Moyano, Francisco Ramiro
Dentice Maidana, Stefania
Kitazawa, Haruki
Villena, Julio Cesar
author_role author
author2 Albarracín, Leonardo Miguel
Raya Tonetti, María Fernanda
Ortiz Moyano, Francisco Ramiro
Dentice Maidana, Stefania
Kitazawa, Haruki
Villena, Julio Cesar
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv LIGILACTOBACILLUS SALIVARIUS
WAKAME ASSIMILATION
PIGS
PROBIOTICS
topic LIGILACTOBACILLUS SALIVARIUS
WAKAME ASSIMILATION
PIGS
PROBIOTICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Wakame (Undaria pinnatifida) is a brown alga widely consumed as edible seaweed in Asia. Wakame contains some bioactive components including fucoidan, alginic acid, omega 3 fatty acids, vitamins, and minerals, which has been reported to have beneficial effects in the human host. Among the beneficial effects attributed to wakame are its antibacterial, antioxidant and anti-inflammatory functions. Recently, we demonstrated that feeding pigs with wakame improved gastrointestinal immunity and induced a significant increase in the abundance of members of the Lactobacillus family, specially Ligilactobacillus salivarius. To determine whether the beneficial immunological effects of wakame were associated to the increment of L. salivarius strains, we isolated and characterized strains from wakame-fed pigs belonging to this species. The complete genome of eight selected L. salivarius strains (named FFIG) were sequenced and the functional and genomic characterization of those strains revealed that their immunomodulatory and adhesion capabilities are a strain-specific characteristic. In this work we aimed to further characterize the L. salivarius FFIG strains studying their wakame assimilation abilities by phenotypic and genotypic approaches. A prolonged fermentation experiment was designed to investigate the consumption of the saccharides by the FFIG strains in two different wakame broths (wakame leaf and wakame stalk). TLC analysis showed that the eight FFIG strains were able to utilize the saccharides contained in enzyme-treated wakame. The strains had different preferences for the two wakame broths. In wakame leaf, FFIG58, FFIG24 and FFIG63 were the strains with the highest ability to grow, whereas FFIG130 and FFIG79 were the strains with the lowest bacterial counts. In wakame stalk, the strains FFIG60, FFIG79 and FFIG130 stood out over the eight strains with the highest viable counts, whereas the FFIG58 have the lowest counts. It was reported that L. salivarius strains have different abundance of genes belonging to glycosyltransferases and glycosylhydrolases families, and that the set of enzymes determine the carbon sources that each strain can use for growing. Then, we evaluated the abundance of genes belonging to glycosylhydrolases families among the FFIG strains and compared them with other L. salivarius strains of animal origin. The clustering analysis considering the numbers and types of glycosylhydrolases showed that strains FFIG58, FFIG63, FFIG79 and FFIG124 had a significant higher abundance of enzymes from the families GH25 and GH13, when compared with all the other L. salivarius strains. Finally, we performed comparative genomic studies between the FFIG strains and other strains isolated from human and porcine origins. We found a core genome compound of 710 genes, and there was no correlation between the number of unique genes and the difference in the phenotypes. To the best of our knowledge, this is the first study that evaluated wakame assimilation capacity of L. salivarius strains using phenotypic and genomic approaches.
Fil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Dentice Maidana, Stefania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
7mo Simposio Argentino de Jóvenes Investigadores en Bioinformática
San Miguel de Tucumán
Argentina
Argentine Regional Student Group
Universidad Nacional de Tucumán. Facultad de Bioquímica Química y Farmacia
description Wakame (Undaria pinnatifida) is a brown alga widely consumed as edible seaweed in Asia. Wakame contains some bioactive components including fucoidan, alginic acid, omega 3 fatty acids, vitamins, and minerals, which has been reported to have beneficial effects in the human host. Among the beneficial effects attributed to wakame are its antibacterial, antioxidant and anti-inflammatory functions. Recently, we demonstrated that feeding pigs with wakame improved gastrointestinal immunity and induced a significant increase in the abundance of members of the Lactobacillus family, specially Ligilactobacillus salivarius. To determine whether the beneficial immunological effects of wakame were associated to the increment of L. salivarius strains, we isolated and characterized strains from wakame-fed pigs belonging to this species. The complete genome of eight selected L. salivarius strains (named FFIG) were sequenced and the functional and genomic characterization of those strains revealed that their immunomodulatory and adhesion capabilities are a strain-specific characteristic. In this work we aimed to further characterize the L. salivarius FFIG strains studying their wakame assimilation abilities by phenotypic and genotypic approaches. A prolonged fermentation experiment was designed to investigate the consumption of the saccharides by the FFIG strains in two different wakame broths (wakame leaf and wakame stalk). TLC analysis showed that the eight FFIG strains were able to utilize the saccharides contained in enzyme-treated wakame. The strains had different preferences for the two wakame broths. In wakame leaf, FFIG58, FFIG24 and FFIG63 were the strains with the highest ability to grow, whereas FFIG130 and FFIG79 were the strains with the lowest bacterial counts. In wakame stalk, the strains FFIG60, FFIG79 and FFIG130 stood out over the eight strains with the highest viable counts, whereas the FFIG58 have the lowest counts. It was reported that L. salivarius strains have different abundance of genes belonging to glycosyltransferases and glycosylhydrolases families, and that the set of enzymes determine the carbon sources that each strain can use for growing. Then, we evaluated the abundance of genes belonging to glycosylhydrolases families among the FFIG strains and compared them with other L. salivarius strains of animal origin. The clustering analysis considering the numbers and types of glycosylhydrolases showed that strains FFIG58, FFIG63, FFIG79 and FFIG124 had a significant higher abundance of enzymes from the families GH25 and GH13, when compared with all the other L. salivarius strains. Finally, we performed comparative genomic studies between the FFIG strains and other strains isolated from human and porcine origins. We found a core genome compound of 710 genes, and there was no correlation between the number of unique genes and the difference in the phenotypes. To the best of our knowledge, this is the first study that evaluated wakame assimilation capacity of L. salivarius strains using phenotypic and genomic approaches.
publishDate 2022
dc.date.none.fl_str_mv 2022
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215982
Phenotypic and genotypic evaluation of wakame assimilation ability in Ligilactobacillus salivarius strains isolated from wakame fed pigs; 7mo Simposio Argentino de Jóvenes Investigadores en Bioinformática; San Miguel de Tucumán; Argentina; 2022; 25-25
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215982
identifier_str_mv Phenotypic and genotypic evaluation of wakame assimilation ability in Ligilactobacillus salivarius strains isolated from wakame fed pigs; 7mo Simposio Argentino de Jóvenes Investigadores en Bioinformática; San Miguel de Tucumán; Argentina; 2022; 25-25
CONICET Digital
CONICET
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