How low does the oxygen concentration go within a sandwich-type amperometric biosensor? Part 2: Theory for PPO biosensors

Autores
Garay, Fernando Sebastian
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A mathematical model to describe the amperometric response of a sandwich-type biosensor containing polyphenol oxidase as the recognition catalytic element is proposed. The model was solved numerically and the resulting nonlinear solution was used to simulate chronoamperometric curves as well as to estimate the concentration profiles of reagents and products of the enzymatic reaction within the sensor. Fromm's systematic method was applied to get a kinetic expression for the analysis of polyphenol oxidase enzymes. The simulated data are compared with curves corresponding to a biosensor prepared with an oxidase enzyme that reacts according to a ping-pong mechanism. Although in both cases oxygen is used as mediator of the enzymatic reaction, the electrochemical step can generate it back from the H2O2 released by enzymes such as glucose oxidase but it cannot do the same in the case of working with polyphenol oxidase. Most of the calculated profiles and related data are presented using dimensioned variables so that they can be directly compared with experimental results. Relevant parameters such as limit current, response-time, and sensitivity are analyzed as function of the thickness of membranes, concentration of enzyme and concentration of substrate.
Fil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
AMPEROMETRIC SENSOR
MATHEMATICAL MODELING
PING-PONG
POLYPHENOL OXIDASE
PPO
SANDWICH-TYPE BIOSENSOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/133801
Acceder
id CONICETDig_0687630119069103b63904589175d78f
oai_identifier_str oai:ri.conicet.gov.ar:11336/133801
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling How low does the oxygen concentration go within a sandwich-type amperometric biosensor? Part 2: Theory for PPO biosensorsGaray, Fernando SebastianAMPEROMETRIC SENSORMATHEMATICAL MODELINGPING-PONGPOLYPHENOL OXIDASEPPOSANDWICH-TYPE BIOSENSORhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A mathematical model to describe the amperometric response of a sandwich-type biosensor containing polyphenol oxidase as the recognition catalytic element is proposed. The model was solved numerically and the resulting nonlinear solution was used to simulate chronoamperometric curves as well as to estimate the concentration profiles of reagents and products of the enzymatic reaction within the sensor. Fromm's systematic method was applied to get a kinetic expression for the analysis of polyphenol oxidase enzymes. The simulated data are compared with curves corresponding to a biosensor prepared with an oxidase enzyme that reacts according to a ping-pong mechanism. Although in both cases oxygen is used as mediator of the enzymatic reaction, the electrochemical step can generate it back from the H2O2 released by enzymes such as glucose oxidase but it cannot do the same in the case of working with polyphenol oxidase. Most of the calculated profiles and related data are presented using dimensioned variables so that they can be directly compared with experimental results. Relevant parameters such as limit current, response-time, and sensitivity are analyzed as function of the thickness of membranes, concentration of enzyme and concentration of substrate.Fil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaElsevier Science SA2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/133801Garay, Fernando Sebastian; How low does the oxygen concentration go within a sandwich-type amperometric biosensor? Part 2: Theory for PPO biosensors; Elsevier Science SA; Sensors and Actuators B: Chemical; 207; 2-2015; 581-5870925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2014.10.105info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0925400514013173info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:11Zoai:ri.conicet.gov.ar:11336/133801instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:11.758CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv How low does the oxygen concentration go within a sandwich-type amperometric biosensor? Part 2: Theory for PPO biosensors
title How low does the oxygen concentration go within a sandwich-type amperometric biosensor? Part 2: Theory for PPO biosensors
spellingShingle How low does the oxygen concentration go within a sandwich-type amperometric biosensor? Part 2: Theory for PPO biosensors
Garay, Fernando Sebastian
AMPEROMETRIC SENSOR
MATHEMATICAL MODELING
PING-PONG
POLYPHENOL OXIDASE
PPO
SANDWICH-TYPE BIOSENSOR
title_short How low does the oxygen concentration go within a sandwich-type amperometric biosensor? Part 2: Theory for PPO biosensors
title_full How low does the oxygen concentration go within a sandwich-type amperometric biosensor? Part 2: Theory for PPO biosensors
title_fullStr How low does the oxygen concentration go within a sandwich-type amperometric biosensor? Part 2: Theory for PPO biosensors
title_full_unstemmed How low does the oxygen concentration go within a sandwich-type amperometric biosensor? Part 2: Theory for PPO biosensors
title_sort How low does the oxygen concentration go within a sandwich-type amperometric biosensor? Part 2: Theory for PPO biosensors
dc.creator.none.fl_str_mv Garay, Fernando Sebastian
author Garay, Fernando Sebastian
author_facet Garay, Fernando Sebastian
author_role author
dc.subject.none.fl_str_mv AMPEROMETRIC SENSOR
MATHEMATICAL MODELING
PING-PONG
POLYPHENOL OXIDASE
PPO
SANDWICH-TYPE BIOSENSOR
topic AMPEROMETRIC SENSOR
MATHEMATICAL MODELING
PING-PONG
POLYPHENOL OXIDASE
PPO
SANDWICH-TYPE BIOSENSOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A mathematical model to describe the amperometric response of a sandwich-type biosensor containing polyphenol oxidase as the recognition catalytic element is proposed. The model was solved numerically and the resulting nonlinear solution was used to simulate chronoamperometric curves as well as to estimate the concentration profiles of reagents and products of the enzymatic reaction within the sensor. Fromm's systematic method was applied to get a kinetic expression for the analysis of polyphenol oxidase enzymes. The simulated data are compared with curves corresponding to a biosensor prepared with an oxidase enzyme that reacts according to a ping-pong mechanism. Although in both cases oxygen is used as mediator of the enzymatic reaction, the electrochemical step can generate it back from the H2O2 released by enzymes such as glucose oxidase but it cannot do the same in the case of working with polyphenol oxidase. Most of the calculated profiles and related data are presented using dimensioned variables so that they can be directly compared with experimental results. Relevant parameters such as limit current, response-time, and sensitivity are analyzed as function of the thickness of membranes, concentration of enzyme and concentration of substrate.
Fil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description A mathematical model to describe the amperometric response of a sandwich-type biosensor containing polyphenol oxidase as the recognition catalytic element is proposed. The model was solved numerically and the resulting nonlinear solution was used to simulate chronoamperometric curves as well as to estimate the concentration profiles of reagents and products of the enzymatic reaction within the sensor. Fromm's systematic method was applied to get a kinetic expression for the analysis of polyphenol oxidase enzymes. The simulated data are compared with curves corresponding to a biosensor prepared with an oxidase enzyme that reacts according to a ping-pong mechanism. Although in both cases oxygen is used as mediator of the enzymatic reaction, the electrochemical step can generate it back from the H2O2 released by enzymes such as glucose oxidase but it cannot do the same in the case of working with polyphenol oxidase. Most of the calculated profiles and related data are presented using dimensioned variables so that they can be directly compared with experimental results. Relevant parameters such as limit current, response-time, and sensitivity are analyzed as function of the thickness of membranes, concentration of enzyme and concentration of substrate.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/133801
Garay, Fernando Sebastian; How low does the oxygen concentration go within a sandwich-type amperometric biosensor? Part 2: Theory for PPO biosensors; Elsevier Science SA; Sensors and Actuators B: Chemical; 207; 2-2015; 581-587
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/133801
identifier_str_mv Garay, Fernando Sebastian; How low does the oxygen concentration go within a sandwich-type amperometric biosensor? Part 2: Theory for PPO biosensors; Elsevier Science SA; Sensors and Actuators B: Chemical; 207; 2-2015; 581-587
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2014.10.105
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0925400514013173
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science SA
publisher.none.fl_str_mv Elsevier Science SA
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614262442426368
score 13.070432

Publicaciones similares