Enhancing Boron Neutron Capture Therapy (BNCT) with Materials Based on COSAN-Functionalized Nanoparticles

Autores
Ferrer Ugalde, Albert; Muñoz Juan, Amanda; Laromaine, Anna; Curotto, Paula; Nievas, Susana Isabel; Dagrosa, Maria Alejandra; Couto, Marcos; Núñez, Rosario
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background/Objectives: Boron neutron capture therapy (BNCT) is a promisingapproach for selectively targeting and destroying malignant cells using 10B isotopes. Asignificant challenge in BNCT lies in the development of efficient boron delivery systemsthat ensure adequate boron accumulation within tumor cells. This study aims to synthesize,characterize, and evaluate COSAN-functionalized nanoparticles (NP@I-COSAN) as apotential boron carrier for BNCT. Methods: Hybrid nanoparticles were synthesized byconjugating monoiodinated cobaltabisdicarbollides (I-COSAN) to commercially availableacrylic polymer-based nanoparticles. Functionalization and cellular uptake were confirmedthrough FTIR, TGA, UV-Vis spectroscopy, and TEM/EDX analyses. Biocompatibilitywas evaluated by assessing cytotoxicity in HeLa cells and C. elegans as an in vivo model.Intracellular boron uptake was quantified using ICP-MS, with results compared to thoseobtained with 4-borono-L-phenylalanine conjugated to fructose (f-BPA). An in vitro BNCTproof-of-concept assay was also performed to evaluate therapeutic efficacy. Results: NP@ICOSAN demonstrated low cytotoxicity and efficient internalization in cells. ICP-MSanalysis revealed stable boron retention, comparable to traditional boron agents. The BNCTassay further showed that NP@I-COSAN was effective in inducing tumor cell apoptosis,even at lower boron concentrations than conventional treatments. Conclusions: Theseresults underscore the potential of NP@I-COSAN as an effective boron delivery system forBNCT, offering a promising strategy to enhance boron accumulation within tumor cellsand improve treatment efficacy.
Fil: Ferrer Ugalde, Albert. No especifíca;
Fil: Muñoz Juan, Amanda. No especifíca;
Fil: Laromaine, Anna. No especifíca;
Fil: Curotto, Paula. Comisión Nacional de Energía Atómica; Argentina
Fil: Nievas, Susana Isabel. Comisión Nacional de Energía Atómica; Argentina
Fil: Dagrosa, Maria Alejandra. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Couto, Marcos. Universidad de la República. Facultad de Ciencias; Uruguay
Fil: Núñez, Rosario. No especifíca;
Materia
boron clusters
BNCT
metallacarboranes
C. elegans
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/266527
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/266527
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enhancing Boron Neutron Capture Therapy (BNCT) with Materials Based on COSAN-Functionalized NanoparticlesFerrer Ugalde, AlbertMuñoz Juan, AmandaLaromaine, AnnaCurotto, PaulaNievas, Susana IsabelDagrosa, Maria AlejandraCouto, MarcosNúñez, Rosarioboron clustersBNCTmetallacarboranesC. eleganshttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Background/Objectives: Boron neutron capture therapy (BNCT) is a promisingapproach for selectively targeting and destroying malignant cells using 10B isotopes. Asignificant challenge in BNCT lies in the development of efficient boron delivery systemsthat ensure adequate boron accumulation within tumor cells. This study aims to synthesize,characterize, and evaluate COSAN-functionalized nanoparticles (NP@I-COSAN) as apotential boron carrier for BNCT. Methods: Hybrid nanoparticles were synthesized byconjugating monoiodinated cobaltabisdicarbollides (I-COSAN) to commercially availableacrylic polymer-based nanoparticles. Functionalization and cellular uptake were confirmedthrough FTIR, TGA, UV-Vis spectroscopy, and TEM/EDX analyses. Biocompatibilitywas evaluated by assessing cytotoxicity in HeLa cells and C. elegans as an in vivo model.Intracellular boron uptake was quantified using ICP-MS, with results compared to thoseobtained with 4-borono-L-phenylalanine conjugated to fructose (f-BPA). An in vitro BNCTproof-of-concept assay was also performed to evaluate therapeutic efficacy. Results: NP@ICOSAN demonstrated low cytotoxicity and efficient internalization in cells. ICP-MSanalysis revealed stable boron retention, comparable to traditional boron agents. The BNCTassay further showed that NP@I-COSAN was effective in inducing tumor cell apoptosis,even at lower boron concentrations than conventional treatments. Conclusions: Theseresults underscore the potential of NP@I-COSAN as an effective boron delivery system forBNCT, offering a promising strategy to enhance boron accumulation within tumor cellsand improve treatment efficacy.Fil: Ferrer Ugalde, Albert. No especifíca;Fil: Muñoz Juan, Amanda. No especifíca;Fil: Laromaine, Anna. No especifíca;Fil: Curotto, Paula. Comisión Nacional de Energía Atómica; ArgentinaFil: Nievas, Susana Isabel. Comisión Nacional de Energía Atómica; ArgentinaFil: Dagrosa, Maria Alejandra. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Couto, Marcos. Universidad de la República. Facultad de Ciencias; UruguayFil: Núñez, Rosario. No especifíca;MDPI2025-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/266527Ferrer Ugalde, Albert; Muñoz Juan, Amanda; Laromaine, Anna; Curotto, Paula; Nievas, Susana Isabel; et al.; Enhancing Boron Neutron Capture Therapy (BNCT) with Materials Based on COSAN-Functionalized Nanoparticles; MDPI; Pharmaceuticals; 18; 4; 3-2025; 1-171424-8247CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1424-8247/18/4/466info:eu-repo/semantics/altIdentifier/doi/10.3390/ph18040466info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:57:09Zoai:ri.conicet.gov.ar:11336/266527instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:57:10.016CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enhancing Boron Neutron Capture Therapy (BNCT) with Materials Based on COSAN-Functionalized Nanoparticles
title Enhancing Boron Neutron Capture Therapy (BNCT) with Materials Based on COSAN-Functionalized Nanoparticles
spellingShingle Enhancing Boron Neutron Capture Therapy (BNCT) with Materials Based on COSAN-Functionalized Nanoparticles
Ferrer Ugalde, Albert
boron clusters
BNCT
metallacarboranes
C. elegans
title_short Enhancing Boron Neutron Capture Therapy (BNCT) with Materials Based on COSAN-Functionalized Nanoparticles
title_full Enhancing Boron Neutron Capture Therapy (BNCT) with Materials Based on COSAN-Functionalized Nanoparticles
title_fullStr Enhancing Boron Neutron Capture Therapy (BNCT) with Materials Based on COSAN-Functionalized Nanoparticles
title_full_unstemmed Enhancing Boron Neutron Capture Therapy (BNCT) with Materials Based on COSAN-Functionalized Nanoparticles
title_sort Enhancing Boron Neutron Capture Therapy (BNCT) with Materials Based on COSAN-Functionalized Nanoparticles
dc.creator.none.fl_str_mv Ferrer Ugalde, Albert
Muñoz Juan, Amanda
Laromaine, Anna
Curotto, Paula
Nievas, Susana Isabel
Dagrosa, Maria Alejandra
Couto, Marcos
Núñez, Rosario
author Ferrer Ugalde, Albert
author_facet Ferrer Ugalde, Albert
Muñoz Juan, Amanda
Laromaine, Anna
Curotto, Paula
Nievas, Susana Isabel
Dagrosa, Maria Alejandra
Couto, Marcos
Núñez, Rosario
author_role author
author2 Muñoz Juan, Amanda
Laromaine, Anna
Curotto, Paula
Nievas, Susana Isabel
Dagrosa, Maria Alejandra
Couto, Marcos
Núñez, Rosario
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv boron clusters
BNCT
metallacarboranes
C. elegans
topic boron clusters
BNCT
metallacarboranes
C. elegans
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background/Objectives: Boron neutron capture therapy (BNCT) is a promisingapproach for selectively targeting and destroying malignant cells using 10B isotopes. Asignificant challenge in BNCT lies in the development of efficient boron delivery systemsthat ensure adequate boron accumulation within tumor cells. This study aims to synthesize,characterize, and evaluate COSAN-functionalized nanoparticles (NP@I-COSAN) as apotential boron carrier for BNCT. Methods: Hybrid nanoparticles were synthesized byconjugating monoiodinated cobaltabisdicarbollides (I-COSAN) to commercially availableacrylic polymer-based nanoparticles. Functionalization and cellular uptake were confirmedthrough FTIR, TGA, UV-Vis spectroscopy, and TEM/EDX analyses. Biocompatibilitywas evaluated by assessing cytotoxicity in HeLa cells and C. elegans as an in vivo model.Intracellular boron uptake was quantified using ICP-MS, with results compared to thoseobtained with 4-borono-L-phenylalanine conjugated to fructose (f-BPA). An in vitro BNCTproof-of-concept assay was also performed to evaluate therapeutic efficacy. Results: NP@ICOSAN demonstrated low cytotoxicity and efficient internalization in cells. ICP-MSanalysis revealed stable boron retention, comparable to traditional boron agents. The BNCTassay further showed that NP@I-COSAN was effective in inducing tumor cell apoptosis,even at lower boron concentrations than conventional treatments. Conclusions: Theseresults underscore the potential of NP@I-COSAN as an effective boron delivery system forBNCT, offering a promising strategy to enhance boron accumulation within tumor cellsand improve treatment efficacy.
Fil: Ferrer Ugalde, Albert. No especifíca;
Fil: Muñoz Juan, Amanda. No especifíca;
Fil: Laromaine, Anna. No especifíca;
Fil: Curotto, Paula. Comisión Nacional de Energía Atómica; Argentina
Fil: Nievas, Susana Isabel. Comisión Nacional de Energía Atómica; Argentina
Fil: Dagrosa, Maria Alejandra. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Couto, Marcos. Universidad de la República. Facultad de Ciencias; Uruguay
Fil: Núñez, Rosario. No especifíca;
description Background/Objectives: Boron neutron capture therapy (BNCT) is a promisingapproach for selectively targeting and destroying malignant cells using 10B isotopes. Asignificant challenge in BNCT lies in the development of efficient boron delivery systemsthat ensure adequate boron accumulation within tumor cells. This study aims to synthesize,characterize, and evaluate COSAN-functionalized nanoparticles (NP@I-COSAN) as apotential boron carrier for BNCT. Methods: Hybrid nanoparticles were synthesized byconjugating monoiodinated cobaltabisdicarbollides (I-COSAN) to commercially availableacrylic polymer-based nanoparticles. Functionalization and cellular uptake were confirmedthrough FTIR, TGA, UV-Vis spectroscopy, and TEM/EDX analyses. Biocompatibilitywas evaluated by assessing cytotoxicity in HeLa cells and C. elegans as an in vivo model.Intracellular boron uptake was quantified using ICP-MS, with results compared to thoseobtained with 4-borono-L-phenylalanine conjugated to fructose (f-BPA). An in vitro BNCTproof-of-concept assay was also performed to evaluate therapeutic efficacy. Results: NP@ICOSAN demonstrated low cytotoxicity and efficient internalization in cells. ICP-MSanalysis revealed stable boron retention, comparable to traditional boron agents. The BNCTassay further showed that NP@I-COSAN was effective in inducing tumor cell apoptosis,even at lower boron concentrations than conventional treatments. Conclusions: Theseresults underscore the potential of NP@I-COSAN as an effective boron delivery system forBNCT, offering a promising strategy to enhance boron accumulation within tumor cellsand improve treatment efficacy.
publishDate 2025
dc.date.none.fl_str_mv 2025-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/266527
Ferrer Ugalde, Albert; Muñoz Juan, Amanda; Laromaine, Anna; Curotto, Paula; Nievas, Susana Isabel; et al.; Enhancing Boron Neutron Capture Therapy (BNCT) with Materials Based on COSAN-Functionalized Nanoparticles; MDPI; Pharmaceuticals; 18; 4; 3-2025; 1-17
1424-8247
CONICET Digital
CONICET
url http://hdl.handle.net/11336/266527
identifier_str_mv Ferrer Ugalde, Albert; Muñoz Juan, Amanda; Laromaine, Anna; Curotto, Paula; Nievas, Susana Isabel; et al.; Enhancing Boron Neutron Capture Therapy (BNCT) with Materials Based on COSAN-Functionalized Nanoparticles; MDPI; Pharmaceuticals; 18; 4; 3-2025; 1-17
1424-8247
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1424-8247/18/4/466
info:eu-repo/semantics/altIdentifier/doi/10.3390/ph18040466
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1843606914832793600
score 13.001348

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