Purification and identification of novel antioxidant peptides isolated from geoffroea decorticans seeds with anticoagulant activity

Autores
Cotabarren, Juliana; Ozón, Brenda; Claver, Santiago; Garcia Pardo, Javier; Obregon, Walter David
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Geoffroea decorticans is a xerophilous deciduous tree present in most arid forests of southern South America, which is commonly used in traditional medicine. The seeds of this tree have been previously investigated for their singular chemical composition, but their protein content has been poorly investigated. Herein, we report the isolation, purification, and characterization of a set of thermostable peptides derived from Geoffroea decorticans seeds (GdAPs) with strong antioxidant and anticoagulant activities. The most potent antioxidant peptides showed a half maximal inhibitory concentration (IC50 ) of 35.5 ± 0.3 µg/mL determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH). They also caused a dose-dependent prolongation of the aPTT clotting time with an IC50 value of ~82 µg/mL. Interestingly, MALDI-TOF/MS analysis showed the presence of three major peptides with low molecular weights of 2257.199 Da, 2717.165 Da, and 5422.002 Da. The derived amino-acid sequence of GdAPs revealed their unique structural features, exhibiting homology with various proteins present in the genome of Arachis hypogaea. All in all, our data suggest a direct applicability of GdAPs for pharmaceutical purposes.
Fil: Cotabarren, Juliana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Ozón, Brenda. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Claver, Santiago. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Garcia Pardo, Javier. Universitat Autònoma de Barcelona; España
Fil: Obregon, Walter David. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
ANTICOAGULANT
ANTIOXIDANT
BIOACTIVE COMPOUND
FUNCTIONAL COMPOUND
GEOFFROEA DECORTICANS
REACTIVE OXYGEN SPECIES
THERMOSTABLE PROTEIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/167236
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/167236
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Purification and identification of novel antioxidant peptides isolated from geoffroea decorticans seeds with anticoagulant activityCotabarren, JulianaOzón, BrendaClaver, SantiagoGarcia Pardo, JavierObregon, Walter DavidANTICOAGULANTANTIOXIDANTBIOACTIVE COMPOUNDFUNCTIONAL COMPOUNDGEOFFROEA DECORTICANSREACTIVE OXYGEN SPECIESTHERMOSTABLE PROTEINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Geoffroea decorticans is a xerophilous deciduous tree present in most arid forests of southern South America, which is commonly used in traditional medicine. The seeds of this tree have been previously investigated for their singular chemical composition, but their protein content has been poorly investigated. Herein, we report the isolation, purification, and characterization of a set of thermostable peptides derived from Geoffroea decorticans seeds (GdAPs) with strong antioxidant and anticoagulant activities. The most potent antioxidant peptides showed a half maximal inhibitory concentration (IC50 ) of 35.5 ± 0.3 µg/mL determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH). They also caused a dose-dependent prolongation of the aPTT clotting time with an IC50 value of ~82 µg/mL. Interestingly, MALDI-TOF/MS analysis showed the presence of three major peptides with low molecular weights of 2257.199 Da, 2717.165 Da, and 5422.002 Da. The derived amino-acid sequence of GdAPs revealed their unique structural features, exhibiting homology with various proteins present in the genome of Arachis hypogaea. All in all, our data suggest a direct applicability of GdAPs for pharmaceutical purposes.Fil: Cotabarren, Juliana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Ozón, Brenda. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; ArgentinaFil: Claver, Santiago. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; ArgentinaFil: Garcia Pardo, Javier. Universitat Autònoma de Barcelona; EspañaFil: Obregon, Walter David. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaMultidisciplinary Digital Publishing Institute2021-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/167236Cotabarren, Juliana; Ozón, Brenda; Claver, Santiago; Garcia Pardo, Javier; Obregon, Walter David; Purification and identification of novel antioxidant peptides isolated from geoffroea decorticans seeds with anticoagulant activity; Multidisciplinary Digital Publishing Institute; Pharmaceutics; 13; 8; 8-2021; 1-121999-4923CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4923/13/8/1153info:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics13081153info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:01Zoai:ri.conicet.gov.ar:11336/167236instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:01.59CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Purification and identification of novel antioxidant peptides isolated from geoffroea decorticans seeds with anticoagulant activity
title Purification and identification of novel antioxidant peptides isolated from geoffroea decorticans seeds with anticoagulant activity
spellingShingle Purification and identification of novel antioxidant peptides isolated from geoffroea decorticans seeds with anticoagulant activity
Cotabarren, Juliana
ANTICOAGULANT
ANTIOXIDANT
BIOACTIVE COMPOUND
FUNCTIONAL COMPOUND
GEOFFROEA DECORTICANS
REACTIVE OXYGEN SPECIES
THERMOSTABLE PROTEIN
title_short Purification and identification of novel antioxidant peptides isolated from geoffroea decorticans seeds with anticoagulant activity
title_full Purification and identification of novel antioxidant peptides isolated from geoffroea decorticans seeds with anticoagulant activity
title_fullStr Purification and identification of novel antioxidant peptides isolated from geoffroea decorticans seeds with anticoagulant activity
title_full_unstemmed Purification and identification of novel antioxidant peptides isolated from geoffroea decorticans seeds with anticoagulant activity
title_sort Purification and identification of novel antioxidant peptides isolated from geoffroea decorticans seeds with anticoagulant activity
dc.creator.none.fl_str_mv Cotabarren, Juliana
Ozón, Brenda
Claver, Santiago
Garcia Pardo, Javier
Obregon, Walter David
author Cotabarren, Juliana
author_facet Cotabarren, Juliana
Ozón, Brenda
Claver, Santiago
Garcia Pardo, Javier
Obregon, Walter David
author_role author
author2 Ozón, Brenda
Claver, Santiago
Garcia Pardo, Javier
Obregon, Walter David
author2_role author
author
author
author
dc.subject.none.fl_str_mv ANTICOAGULANT
ANTIOXIDANT
BIOACTIVE COMPOUND
FUNCTIONAL COMPOUND
GEOFFROEA DECORTICANS
REACTIVE OXYGEN SPECIES
THERMOSTABLE PROTEIN
topic ANTICOAGULANT
ANTIOXIDANT
BIOACTIVE COMPOUND
FUNCTIONAL COMPOUND
GEOFFROEA DECORTICANS
REACTIVE OXYGEN SPECIES
THERMOSTABLE PROTEIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Geoffroea decorticans is a xerophilous deciduous tree present in most arid forests of southern South America, which is commonly used in traditional medicine. The seeds of this tree have been previously investigated for their singular chemical composition, but their protein content has been poorly investigated. Herein, we report the isolation, purification, and characterization of a set of thermostable peptides derived from Geoffroea decorticans seeds (GdAPs) with strong antioxidant and anticoagulant activities. The most potent antioxidant peptides showed a half maximal inhibitory concentration (IC50 ) of 35.5 ± 0.3 µg/mL determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH). They also caused a dose-dependent prolongation of the aPTT clotting time with an IC50 value of ~82 µg/mL. Interestingly, MALDI-TOF/MS analysis showed the presence of three major peptides with low molecular weights of 2257.199 Da, 2717.165 Da, and 5422.002 Da. The derived amino-acid sequence of GdAPs revealed their unique structural features, exhibiting homology with various proteins present in the genome of Arachis hypogaea. All in all, our data suggest a direct applicability of GdAPs for pharmaceutical purposes.
Fil: Cotabarren, Juliana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Ozón, Brenda. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Claver, Santiago. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Garcia Pardo, Javier. Universitat Autònoma de Barcelona; España
Fil: Obregon, Walter David. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description Geoffroea decorticans is a xerophilous deciduous tree present in most arid forests of southern South America, which is commonly used in traditional medicine. The seeds of this tree have been previously investigated for their singular chemical composition, but their protein content has been poorly investigated. Herein, we report the isolation, purification, and characterization of a set of thermostable peptides derived from Geoffroea decorticans seeds (GdAPs) with strong antioxidant and anticoagulant activities. The most potent antioxidant peptides showed a half maximal inhibitory concentration (IC50 ) of 35.5 ± 0.3 µg/mL determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH). They also caused a dose-dependent prolongation of the aPTT clotting time with an IC50 value of ~82 µg/mL. Interestingly, MALDI-TOF/MS analysis showed the presence of three major peptides with low molecular weights of 2257.199 Da, 2717.165 Da, and 5422.002 Da. The derived amino-acid sequence of GdAPs revealed their unique structural features, exhibiting homology with various proteins present in the genome of Arachis hypogaea. All in all, our data suggest a direct applicability of GdAPs for pharmaceutical purposes.
publishDate 2021
dc.date.none.fl_str_mv 2021-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/167236
Cotabarren, Juliana; Ozón, Brenda; Claver, Santiago; Garcia Pardo, Javier; Obregon, Walter David; Purification and identification of novel antioxidant peptides isolated from geoffroea decorticans seeds with anticoagulant activity; Multidisciplinary Digital Publishing Institute; Pharmaceutics; 13; 8; 8-2021; 1-12
1999-4923
CONICET Digital
CONICET
url http://hdl.handle.net/11336/167236
identifier_str_mv Cotabarren, Juliana; Ozón, Brenda; Claver, Santiago; Garcia Pardo, Javier; Obregon, Walter David; Purification and identification of novel antioxidant peptides isolated from geoffroea decorticans seeds with anticoagulant activity; Multidisciplinary Digital Publishing Institute; Pharmaceutics; 13; 8; 8-2021; 1-12
1999-4923
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4923/13/8/1153
info:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics13081153
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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