Successful fibrinolytic enzymes production by solid state fermentation

Autores
Rovati, Jose Ignacio; Cabral, Maria Eugenia; Castellanos, Lucia Ines; Fariña, Julia Ines
Año de publicación
2011
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Fibrin accumulation in blood vessels increases thrombosis risks promoting myocardial infarction and cardiovascular diseases. Bionectria sp. LY 4.1, a wild fungi isolated from Las Yungas Pedemontana rainforest, was recently reported by our group as a novel fibrinolytic enzyme source. In the present study we attempted the production of fibrinolytic enzymes by solid state fermentation (SSF) with Bionectria sp. LY 4.1 looking for low-cost substrates and simplified operational conditions. The different solid substrates (SS) evaluated (polyurethane foam - PUF, bagasse, textured soy flour, soy flour pellets and wheat bran), in adequate amounts were inoculated with fresh production medium containing 72 h-old Bionectria sp. culture. The effect of different conditions of moisture (9, 11, 13, 15, 17%), bed height (1, 2, 4, 6, 8 cm) and particle size(0.25; 0.5; 1 and 2 cm ) was studied. Fibrinolytic activity was determined by the fibrin plate technique and protein concentration by the BCA method. The obtained results confirmed the possibility to produce fibrinolytic enzymes by SSF with Bionectria sp. LY 4.1. Furthermore, assays demonstrated the convenience of using PUF as SS under specific operating conditions over the other substrates tested. The findings herein presented offer an interesting alternative for the production of a high added-value pharmacological product.
Fil: Rovati, Jose Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
San Luis
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Materia
FIBRINOLYTIC ENZYMES
SOLID STATE FERMENTATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194927

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network_name_str CONICET Digital (CONICET)
spelling Successful fibrinolytic enzymes production by solid state fermentationRovati, Jose IgnacioCabral, Maria EugeniaCastellanos, Lucia InesFariña, Julia InesFIBRINOLYTIC ENZYMESSOLID STATE FERMENTATIONhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Fibrin accumulation in blood vessels increases thrombosis risks promoting myocardial infarction and cardiovascular diseases. Bionectria sp. LY 4.1, a wild fungi isolated from Las Yungas Pedemontana rainforest, was recently reported by our group as a novel fibrinolytic enzyme source. In the present study we attempted the production of fibrinolytic enzymes by solid state fermentation (SSF) with Bionectria sp. LY 4.1 looking for low-cost substrates and simplified operational conditions. The different solid substrates (SS) evaluated (polyurethane foam - PUF, bagasse, textured soy flour, soy flour pellets and wheat bran), in adequate amounts were inoculated with fresh production medium containing 72 h-old Bionectria sp. culture. The effect of different conditions of moisture (9, 11, 13, 15, 17%), bed height (1, 2, 4, 6, 8 cm) and particle size(0.25; 0.5; 1 and 2 cm ) was studied. Fibrinolytic activity was determined by the fibrin plate technique and protein concentration by the BCA method. The obtained results confirmed the possibility to produce fibrinolytic enzymes by SSF with Bionectria sp. LY 4.1. Furthermore, assays demonstrated the convenience of using PUF as SS under specific operating conditions over the other substrates tested. The findings herein presented offer an interesting alternative for the production of a high added-value pharmacological product.Fil: Rovati, Jose Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaXLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularSan LuisArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularSociedad Argentina de Investigación Bioquímica y Biología Molecular2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/194927Successful fibrinolytic enzymes production by solid state fermentation; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; San Luis; Argentina; 2011; 94-940327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-35.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:46:19Zoai:ri.conicet.gov.ar:11336/194927instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:46:19.781CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Successful fibrinolytic enzymes production by solid state fermentation
title Successful fibrinolytic enzymes production by solid state fermentation
spellingShingle Successful fibrinolytic enzymes production by solid state fermentation
Rovati, Jose Ignacio
FIBRINOLYTIC ENZYMES
SOLID STATE FERMENTATION
title_short Successful fibrinolytic enzymes production by solid state fermentation
title_full Successful fibrinolytic enzymes production by solid state fermentation
title_fullStr Successful fibrinolytic enzymes production by solid state fermentation
title_full_unstemmed Successful fibrinolytic enzymes production by solid state fermentation
title_sort Successful fibrinolytic enzymes production by solid state fermentation
dc.creator.none.fl_str_mv Rovati, Jose Ignacio
Cabral, Maria Eugenia
Castellanos, Lucia Ines
Fariña, Julia Ines
author Rovati, Jose Ignacio
author_facet Rovati, Jose Ignacio
Cabral, Maria Eugenia
Castellanos, Lucia Ines
Fariña, Julia Ines
author_role author
author2 Cabral, Maria Eugenia
Castellanos, Lucia Ines
Fariña, Julia Ines
author2_role author
author
author
dc.subject.none.fl_str_mv FIBRINOLYTIC ENZYMES
SOLID STATE FERMENTATION
topic FIBRINOLYTIC ENZYMES
SOLID STATE FERMENTATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Fibrin accumulation in blood vessels increases thrombosis risks promoting myocardial infarction and cardiovascular diseases. Bionectria sp. LY 4.1, a wild fungi isolated from Las Yungas Pedemontana rainforest, was recently reported by our group as a novel fibrinolytic enzyme source. In the present study we attempted the production of fibrinolytic enzymes by solid state fermentation (SSF) with Bionectria sp. LY 4.1 looking for low-cost substrates and simplified operational conditions. The different solid substrates (SS) evaluated (polyurethane foam - PUF, bagasse, textured soy flour, soy flour pellets and wheat bran), in adequate amounts were inoculated with fresh production medium containing 72 h-old Bionectria sp. culture. The effect of different conditions of moisture (9, 11, 13, 15, 17%), bed height (1, 2, 4, 6, 8 cm) and particle size(0.25; 0.5; 1 and 2 cm ) was studied. Fibrinolytic activity was determined by the fibrin plate technique and protein concentration by the BCA method. The obtained results confirmed the possibility to produce fibrinolytic enzymes by SSF with Bionectria sp. LY 4.1. Furthermore, assays demonstrated the convenience of using PUF as SS under specific operating conditions over the other substrates tested. The findings herein presented offer an interesting alternative for the production of a high added-value pharmacological product.
Fil: Rovati, Jose Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
San Luis
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
description Fibrin accumulation in blood vessels increases thrombosis risks promoting myocardial infarction and cardiovascular diseases. Bionectria sp. LY 4.1, a wild fungi isolated from Las Yungas Pedemontana rainforest, was recently reported by our group as a novel fibrinolytic enzyme source. In the present study we attempted the production of fibrinolytic enzymes by solid state fermentation (SSF) with Bionectria sp. LY 4.1 looking for low-cost substrates and simplified operational conditions. The different solid substrates (SS) evaluated (polyurethane foam - PUF, bagasse, textured soy flour, soy flour pellets and wheat bran), in adequate amounts were inoculated with fresh production medium containing 72 h-old Bionectria sp. culture. The effect of different conditions of moisture (9, 11, 13, 15, 17%), bed height (1, 2, 4, 6, 8 cm) and particle size(0.25; 0.5; 1 and 2 cm ) was studied. Fibrinolytic activity was determined by the fibrin plate technique and protein concentration by the BCA method. The obtained results confirmed the possibility to produce fibrinolytic enzymes by SSF with Bionectria sp. LY 4.1. Furthermore, assays demonstrated the convenience of using PUF as SS under specific operating conditions over the other substrates tested. The findings herein presented offer an interesting alternative for the production of a high added-value pharmacological product.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194927
Successful fibrinolytic enzymes production by solid state fermentation; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; San Luis; Argentina; 2011; 94-94
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194927
identifier_str_mv Successful fibrinolytic enzymes production by solid state fermentation; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; San Luis; Argentina; 2011; 94-94
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/vnd.openxmlformats-officedocument.wordprocessingml.document
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación Bioquímica y Biología Molecular
publisher.none.fl_str_mv Sociedad Argentina de Investigación Bioquímica y Biología Molecular
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
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