Agroindustrial waste from the cuyo region revalorization: preliminary tests using solid state fermentation

Autores
Martin, María Lucia; Rodríguez, Laura A.; Gil, Rocio Mariel; Vallejo, Martha Dina; Gouric, Silvia C.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The agroindustrial activity of the Cuyo region is based mainly on the processing of two crops: the vine and the olive; and to a lesser extent fruits and vegetables. The wine industry and the olive oil industry generate large amounts of solid waste, such as grape marc, grape stalks and olive mark (OM). At present, the final disposal of these residues is difficult due to their high concentrations of phytotoxic substances, causing environmental problems if they accumulate in large quantities. Due to its chemical composition (carbohydrates, fiber, fats, proteins and mineral salts), agro-industrial residues are suitable substrates for biotechnological proceesing to obtain value-added products. The objective of this work was to carry out a preliminary study of the production of hydrolytic enzymes using Aspergillus niger in Solid State Fermentation (SSF) using solid regional wastes. The following residues were used: red grape marc (RGM), olive mark (OM) and mixture RGM-OM (50% w/w of each one). SSF were carried out in Petri´s dishes at 27°C, with solid medium initial moisture content of 60% w/w and an inoculum size of 1x107 spores*g dry-1 of A. niger. Two samples of culture were taken per day during one week for subsequent analytical determinations. The enzymatic activities that were measured in the aqueous extracts of SSF were: α-L-arabinofuranosidase (α-L-arf), α-L-rhamnosidases (α-L-rha), exopolygalacturonase (exo-PG), laccase (lac) and lignin peroxidase (LiP). The maximum enzymatic activity values were obtained for α-L-arf and exo-PG. The maximum activities for both enzymes were: 2.62U/g for α-L-arf, at 58 hours of cultivation and 4.53U/g for exo-PG at 70 hours, in SSF of RGM-OM. As for lac and α-L-rha activities, lower values were obtained. No significant amounts of LiP activity were determined. The results obtained indicate that it is of interest to optimize the SSF tests with the mixture of the two solid substrates to maximize the values of the enzymatic complex, investigating the culture conditions and nutrient additions.
Fil: Martin, María Lucia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Rodríguez, Laura A.. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Gil, Rocio Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Vallejo, Martha Dina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Gouric, Silvia C.. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo
Mendoza
Argentina
Sociedad de Biología de Cuyo
Universidad Nacional de Cuyo. Secretaría de Extensión Universitaria
Materia
SOLID STATE
FERMENTATION
REVALORIZATION
ALPERUJO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/162508
Acceder
id CONICETDig_70916cf5f1236942fc06f0dea40343cd
oai_identifier_str oai:ri.conicet.gov.ar:11336/162508
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Agroindustrial waste from the cuyo region revalorization: preliminary tests using solid state fermentationMartin, María LuciaRodríguez, Laura A.Gil, Rocio MarielVallejo, Martha DinaGouric, Silvia C.SOLID STATEFERMENTATIONREVALORIZATIONALPERUJOhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The agroindustrial activity of the Cuyo region is based mainly on the processing of two crops: the vine and the olive; and to a lesser extent fruits and vegetables. The wine industry and the olive oil industry generate large amounts of solid waste, such as grape marc, grape stalks and olive mark (OM). At present, the final disposal of these residues is difficult due to their high concentrations of phytotoxic substances, causing environmental problems if they accumulate in large quantities. Due to its chemical composition (carbohydrates, fiber, fats, proteins and mineral salts), agro-industrial residues are suitable substrates for biotechnological proceesing to obtain value-added products. The objective of this work was to carry out a preliminary study of the production of hydrolytic enzymes using Aspergillus niger in Solid State Fermentation (SSF) using solid regional wastes. The following residues were used: red grape marc (RGM), olive mark (OM) and mixture RGM-OM (50% w/w of each one). SSF were carried out in Petri´s dishes at 27°C, with solid medium initial moisture content of 60% w/w and an inoculum size of 1x107 spores*g dry-1 of A. niger. Two samples of culture were taken per day during one week for subsequent analytical determinations. The enzymatic activities that were measured in the aqueous extracts of SSF were: α-L-arabinofuranosidase (α-L-arf), α-L-rhamnosidases (α-L-rha), exopolygalacturonase (exo-PG), laccase (lac) and lignin peroxidase (LiP). The maximum enzymatic activity values were obtained for α-L-arf and exo-PG. The maximum activities for both enzymes were: 2.62U/g for α-L-arf, at 58 hours of cultivation and 4.53U/g for exo-PG at 70 hours, in SSF of RGM-OM. As for lac and α-L-rha activities, lower values were obtained. No significant amounts of LiP activity were determined. The results obtained indicate that it is of interest to optimize the SSF tests with the mixture of the two solid substrates to maximize the values of the enzymatic complex, investigating the culture conditions and nutrient additions.Fil: Martin, María Lucia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Rodríguez, Laura A.. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Gil, Rocio Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Vallejo, Martha Dina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Gouric, Silvia C.. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaXXXVI Reunión Científica Anual de la Sociedad de Biología de CuyoMendozaArgentinaSociedad de Biología de CuyoUniversidad Nacional de Cuyo. Secretaría de Extensión UniversitariaUniversidad Nacional de Cuyo. Secretaría de Extensión Universitaria2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/162508Agroindustrial waste from the cuyo region revalorization: preliminary tests using solid state fermentation; XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo; Mendoza; Argentina; 2018; 96-97CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/libro-de-resumenes-2018/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:27:47Zoai:ri.conicet.gov.ar:11336/162508instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:27:47.87CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Agroindustrial waste from the cuyo region revalorization: preliminary tests using solid state fermentation
title Agroindustrial waste from the cuyo region revalorization: preliminary tests using solid state fermentation
spellingShingle Agroindustrial waste from the cuyo region revalorization: preliminary tests using solid state fermentation
Martin, María Lucia
SOLID STATE
FERMENTATION
REVALORIZATION
ALPERUJO
title_short Agroindustrial waste from the cuyo region revalorization: preliminary tests using solid state fermentation
title_full Agroindustrial waste from the cuyo region revalorization: preliminary tests using solid state fermentation
title_fullStr Agroindustrial waste from the cuyo region revalorization: preliminary tests using solid state fermentation
title_full_unstemmed Agroindustrial waste from the cuyo region revalorization: preliminary tests using solid state fermentation
title_sort Agroindustrial waste from the cuyo region revalorization: preliminary tests using solid state fermentation
dc.creator.none.fl_str_mv Martin, María Lucia
Rodríguez, Laura A.
Gil, Rocio Mariel
Vallejo, Martha Dina
Gouric, Silvia C.
author Martin, María Lucia
author_facet Martin, María Lucia
Rodríguez, Laura A.
Gil, Rocio Mariel
Vallejo, Martha Dina
Gouric, Silvia C.
author_role author
author2 Rodríguez, Laura A.
Gil, Rocio Mariel
Vallejo, Martha Dina
Gouric, Silvia C.
author2_role author
author
author
author
dc.subject.none.fl_str_mv SOLID STATE
FERMENTATION
REVALORIZATION
ALPERUJO
topic SOLID STATE
FERMENTATION
REVALORIZATION
ALPERUJO
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The agroindustrial activity of the Cuyo region is based mainly on the processing of two crops: the vine and the olive; and to a lesser extent fruits and vegetables. The wine industry and the olive oil industry generate large amounts of solid waste, such as grape marc, grape stalks and olive mark (OM). At present, the final disposal of these residues is difficult due to their high concentrations of phytotoxic substances, causing environmental problems if they accumulate in large quantities. Due to its chemical composition (carbohydrates, fiber, fats, proteins and mineral salts), agro-industrial residues are suitable substrates for biotechnological proceesing to obtain value-added products. The objective of this work was to carry out a preliminary study of the production of hydrolytic enzymes using Aspergillus niger in Solid State Fermentation (SSF) using solid regional wastes. The following residues were used: red grape marc (RGM), olive mark (OM) and mixture RGM-OM (50% w/w of each one). SSF were carried out in Petri´s dishes at 27°C, with solid medium initial moisture content of 60% w/w and an inoculum size of 1x107 spores*g dry-1 of A. niger. Two samples of culture were taken per day during one week for subsequent analytical determinations. The enzymatic activities that were measured in the aqueous extracts of SSF were: α-L-arabinofuranosidase (α-L-arf), α-L-rhamnosidases (α-L-rha), exopolygalacturonase (exo-PG), laccase (lac) and lignin peroxidase (LiP). The maximum enzymatic activity values were obtained for α-L-arf and exo-PG. The maximum activities for both enzymes were: 2.62U/g for α-L-arf, at 58 hours of cultivation and 4.53U/g for exo-PG at 70 hours, in SSF of RGM-OM. As for lac and α-L-rha activities, lower values were obtained. No significant amounts of LiP activity were determined. The results obtained indicate that it is of interest to optimize the SSF tests with the mixture of the two solid substrates to maximize the values of the enzymatic complex, investigating the culture conditions and nutrient additions.
Fil: Martin, María Lucia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Rodríguez, Laura A.. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Gil, Rocio Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Vallejo, Martha Dina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
Fil: Gouric, Silvia C.. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina
XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo
Mendoza
Argentina
Sociedad de Biología de Cuyo
Universidad Nacional de Cuyo. Secretaría de Extensión Universitaria
description The agroindustrial activity of the Cuyo region is based mainly on the processing of two crops: the vine and the olive; and to a lesser extent fruits and vegetables. The wine industry and the olive oil industry generate large amounts of solid waste, such as grape marc, grape stalks and olive mark (OM). At present, the final disposal of these residues is difficult due to their high concentrations of phytotoxic substances, causing environmental problems if they accumulate in large quantities. Due to its chemical composition (carbohydrates, fiber, fats, proteins and mineral salts), agro-industrial residues are suitable substrates for biotechnological proceesing to obtain value-added products. The objective of this work was to carry out a preliminary study of the production of hydrolytic enzymes using Aspergillus niger in Solid State Fermentation (SSF) using solid regional wastes. The following residues were used: red grape marc (RGM), olive mark (OM) and mixture RGM-OM (50% w/w of each one). SSF were carried out in Petri´s dishes at 27°C, with solid medium initial moisture content of 60% w/w and an inoculum size of 1x107 spores*g dry-1 of A. niger. Two samples of culture were taken per day during one week for subsequent analytical determinations. The enzymatic activities that were measured in the aqueous extracts of SSF were: α-L-arabinofuranosidase (α-L-arf), α-L-rhamnosidases (α-L-rha), exopolygalacturonase (exo-PG), laccase (lac) and lignin peroxidase (LiP). The maximum enzymatic activity values were obtained for α-L-arf and exo-PG. The maximum activities for both enzymes were: 2.62U/g for α-L-arf, at 58 hours of cultivation and 4.53U/g for exo-PG at 70 hours, in SSF of RGM-OM. As for lac and α-L-rha activities, lower values were obtained. No significant amounts of LiP activity were determined. The results obtained indicate that it is of interest to optimize the SSF tests with the mixture of the two solid substrates to maximize the values of the enzymatic complex, investigating the culture conditions and nutrient additions.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/162508
Agroindustrial waste from the cuyo region revalorization: preliminary tests using solid state fermentation; XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo; Mendoza; Argentina; 2018; 96-97
CONICET Digital
CONICET
url http://hdl.handle.net/11336/162508
identifier_str_mv Agroindustrial waste from the cuyo region revalorization: preliminary tests using solid state fermentation; XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo; Mendoza; Argentina; 2018; 96-97
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/libro-de-resumenes-2018/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Universidad Nacional de Cuyo. Secretaría de Extensión Universitaria
publisher.none.fl_str_mv Universidad Nacional de Cuyo. Secretaría de Extensión Universitaria
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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