Optimized lovastatin production by solid-state fermentation using agro-industrial derived substrates

Autores
Cabral, Maria Eugenia; Joya, Constanza María; Castellanos, Lucia Ines; Fariña, Julia Ines
Año de publicación
2011
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Lovastatin is a drug that can inhibit the de novo synthesis of cholesterol by competitive inhibition of HMG-CoA reductase, which catalyzes the rate limiting step of cholesterol biosynthesis. It can be then effectively used for the hypercholesterolemia treatment. Nowadays, interest has focused in searching new statins and/or developing low-cost production strategies. This work aimed at optimizing lovastatin production by solid state fermentation (SSF) with Aspergillus terreus MEC, looking for low-cost and readily available substrates. Cultures consisted in textured deffated soy bean flour (HSDT) embedded with A. terreus-inoculated LP (whey protein concentrate) or SQ (whey) liquid media. Different initial pHs (4.3, 5.3, 6.3, 7.3), incubation temperatures (23, 25, 28, 30°C), moisture contents (CHI: 36, 46, 56, 66, 76%) and bed heights (1, 1.5, 2, 2.5, 3, 4 cm) were evaluated. Extracted lovastatin was analyzed by RP-HPLC with a Diode Array Detector and fungal growth was indirectly determined by ergosterol quantification. A lovastatin production of 1017.55 µg per g of dry weight was reached by using optimized SSF consisting in SQ liquid medium adsorbed on HSDT at an incubation temperature of 25°C, pH = 6.3, CHI = 46-56% and a bed height of 1.5-2.5 cm. These results highlight the relevance of SSF strategies for the production of high-added-value secondary metabolites.
Fil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Joya, Constanza María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
San Luis
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Materia
LOVASTATIN
SOLID STATE FERMENTATION
AGRO-INDUSTRIAL SUBSTRATES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194926
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/194926
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Optimized lovastatin production by solid-state fermentation using agro-industrial derived substratesCabral, Maria EugeniaJoya, Constanza MaríaCastellanos, Lucia InesFariña, Julia InesLOVASTATINSOLID STATE FERMENTATIONAGRO-INDUSTRIAL SUBSTRATEShttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Lovastatin is a drug that can inhibit the de novo synthesis of cholesterol by competitive inhibition of HMG-CoA reductase, which catalyzes the rate limiting step of cholesterol biosynthesis. It can be then effectively used for the hypercholesterolemia treatment. Nowadays, interest has focused in searching new statins and/or developing low-cost production strategies. This work aimed at optimizing lovastatin production by solid state fermentation (SSF) with Aspergillus terreus MEC, looking for low-cost and readily available substrates. Cultures consisted in textured deffated soy bean flour (HSDT) embedded with A. terreus-inoculated LP (whey protein concentrate) or SQ (whey) liquid media. Different initial pHs (4.3, 5.3, 6.3, 7.3), incubation temperatures (23, 25, 28, 30°C), moisture contents (CHI: 36, 46, 56, 66, 76%) and bed heights (1, 1.5, 2, 2.5, 3, 4 cm) were evaluated. Extracted lovastatin was analyzed by RP-HPLC with a Diode Array Detector and fungal growth was indirectly determined by ergosterol quantification. A lovastatin production of 1017.55 µg per g of dry weight was reached by using optimized SSF consisting in SQ liquid medium adsorbed on HSDT at an incubation temperature of 25°C, pH = 6.3, CHI = 46-56% and a bed height of 1.5-2.5 cm. These results highlight the relevance of SSF strategies for the production of high-added-value secondary metabolites.Fil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Joya, Constanza María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaXLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularSan LuisArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularSociedad Argentina de Investigación en Bioquímica y Biología Molecular2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/194926Optimized lovastatin production by solid-state fermentation using agro-industrial derived substrates; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; San Luis; Argentina; 2011; 94-940327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://saib.org.ar/archivos/biocell-35.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:22:27Zoai:ri.conicet.gov.ar:11336/194926instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:22:27.89CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Optimized lovastatin production by solid-state fermentation using agro-industrial derived substrates
title Optimized lovastatin production by solid-state fermentation using agro-industrial derived substrates
spellingShingle Optimized lovastatin production by solid-state fermentation using agro-industrial derived substrates
Cabral, Maria Eugenia
LOVASTATIN
SOLID STATE FERMENTATION
AGRO-INDUSTRIAL SUBSTRATES
title_short Optimized lovastatin production by solid-state fermentation using agro-industrial derived substrates
title_full Optimized lovastatin production by solid-state fermentation using agro-industrial derived substrates
title_fullStr Optimized lovastatin production by solid-state fermentation using agro-industrial derived substrates
title_full_unstemmed Optimized lovastatin production by solid-state fermentation using agro-industrial derived substrates
title_sort Optimized lovastatin production by solid-state fermentation using agro-industrial derived substrates
dc.creator.none.fl_str_mv Cabral, Maria Eugenia
Joya, Constanza María
Castellanos, Lucia Ines
Fariña, Julia Ines
author Cabral, Maria Eugenia
author_facet Cabral, Maria Eugenia
Joya, Constanza María
Castellanos, Lucia Ines
Fariña, Julia Ines
author_role author
author2 Joya, Constanza María
Castellanos, Lucia Ines
Fariña, Julia Ines
author2_role author
author
author
dc.subject.none.fl_str_mv LOVASTATIN
SOLID STATE FERMENTATION
AGRO-INDUSTRIAL SUBSTRATES
topic LOVASTATIN
SOLID STATE FERMENTATION
AGRO-INDUSTRIAL SUBSTRATES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Lovastatin is a drug that can inhibit the de novo synthesis of cholesterol by competitive inhibition of HMG-CoA reductase, which catalyzes the rate limiting step of cholesterol biosynthesis. It can be then effectively used for the hypercholesterolemia treatment. Nowadays, interest has focused in searching new statins and/or developing low-cost production strategies. This work aimed at optimizing lovastatin production by solid state fermentation (SSF) with Aspergillus terreus MEC, looking for low-cost and readily available substrates. Cultures consisted in textured deffated soy bean flour (HSDT) embedded with A. terreus-inoculated LP (whey protein concentrate) or SQ (whey) liquid media. Different initial pHs (4.3, 5.3, 6.3, 7.3), incubation temperatures (23, 25, 28, 30°C), moisture contents (CHI: 36, 46, 56, 66, 76%) and bed heights (1, 1.5, 2, 2.5, 3, 4 cm) were evaluated. Extracted lovastatin was analyzed by RP-HPLC with a Diode Array Detector and fungal growth was indirectly determined by ergosterol quantification. A lovastatin production of 1017.55 µg per g of dry weight was reached by using optimized SSF consisting in SQ liquid medium adsorbed on HSDT at an incubation temperature of 25°C, pH = 6.3, CHI = 46-56% and a bed height of 1.5-2.5 cm. These results highlight the relevance of SSF strategies for the production of high-added-value secondary metabolites.
Fil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Joya, Constanza María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
San Luis
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
description Lovastatin is a drug that can inhibit the de novo synthesis of cholesterol by competitive inhibition of HMG-CoA reductase, which catalyzes the rate limiting step of cholesterol biosynthesis. It can be then effectively used for the hypercholesterolemia treatment. Nowadays, interest has focused in searching new statins and/or developing low-cost production strategies. This work aimed at optimizing lovastatin production by solid state fermentation (SSF) with Aspergillus terreus MEC, looking for low-cost and readily available substrates. Cultures consisted in textured deffated soy bean flour (HSDT) embedded with A. terreus-inoculated LP (whey protein concentrate) or SQ (whey) liquid media. Different initial pHs (4.3, 5.3, 6.3, 7.3), incubation temperatures (23, 25, 28, 30°C), moisture contents (CHI: 36, 46, 56, 66, 76%) and bed heights (1, 1.5, 2, 2.5, 3, 4 cm) were evaluated. Extracted lovastatin was analyzed by RP-HPLC with a Diode Array Detector and fungal growth was indirectly determined by ergosterol quantification. A lovastatin production of 1017.55 µg per g of dry weight was reached by using optimized SSF consisting in SQ liquid medium adsorbed on HSDT at an incubation temperature of 25°C, pH = 6.3, CHI = 46-56% and a bed height of 1.5-2.5 cm. These results highlight the relevance of SSF strategies for the production of high-added-value secondary metabolites.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194926
Optimized lovastatin production by solid-state fermentation using agro-industrial derived substrates; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; San Luis; Argentina; 2011; 94-94
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194926
identifier_str_mv Optimized lovastatin production by solid-state fermentation using agro-industrial derived substrates; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; San Luis; Argentina; 2011; 94-94
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://saib.org.ar/archivos/biocell-35.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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