A comparative study of the synthesis of n-butyl-oleate using a crude lipolytic extract of Penicillum coryophilum in water-restricted environments

Autores
Baron, Alessandra M.; Sarquis, María Inés M.; Baigori, Mario Domingo; Mitchell, David A.; Krieger, Nadia
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We evaluated the potential of a lipolytic extract from Penicillium corylophilum for use in biocatalysis. Lipolytic activity was stable when the extract was incubated in the presence of hydrophobic solvents. In fact, when the extract was incubated in n-heptane with an initial water activity of 0.95, the activity increased by 30%. Synthesis of n-butyl-oleate in different systems was compared. In AOT/n-heptane reverse micelles, a 100% yield of ester was obtained within 12 h, with a water content (W0, [H2O]/[AOT]) of 10. Several macro-heterogeneous systems involving n-heptane as the solvent were also compared. Direct addition of lyophilised enzymatic preparation to this system gave a 100% yield in 48 h, with an initial water activity of 0.11. Performance was poorer with direct addition of enzymatic preparation co-lyophilised with β-cyclodextrin and with addition of enzymatic preparation immobilized on a hydrophobic gel, with yields significantly less than 100%. We conclude that the lipolytic extract of P. corylophilum can be used in synthesis reactions in low-water systems, which justifies further efforts to purify lipolytic enzymes from the extract and to undertake an in-depth evaluation of their potential for use in biocatalysis, for the production of fine chemicals.
Fil: Baron, Alessandra M.. Universidade Federal do Paraná; Brasil
Fil: Sarquis, María Inés M.. Colecâo de Cultura de Fungos; Brasil
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Mitchell, David A.. Universidade Federal do Paraná; Brasil
Fil: Krieger, Nadia. Universidade Federal do Paraná; Brasil
Materia
Lipases
Penicilium Corylophilum
Biocatalysis
Low-Water Systems
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41516
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41516
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A comparative study of the synthesis of n-butyl-oleate using a crude lipolytic extract of Penicillum coryophilum in water-restricted environmentsBaron, Alessandra M.Sarquis, María Inés M.Baigori, Mario DomingoMitchell, David A.Krieger, NadiaLipasesPenicilium CorylophilumBiocatalysisLow-Water Systemshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We evaluated the potential of a lipolytic extract from Penicillium corylophilum for use in biocatalysis. Lipolytic activity was stable when the extract was incubated in the presence of hydrophobic solvents. In fact, when the extract was incubated in n-heptane with an initial water activity of 0.95, the activity increased by 30%. Synthesis of n-butyl-oleate in different systems was compared. In AOT/n-heptane reverse micelles, a 100% yield of ester was obtained within 12 h, with a water content (W0, [H2O]/[AOT]) of 10. Several macro-heterogeneous systems involving n-heptane as the solvent were also compared. Direct addition of lyophilised enzymatic preparation to this system gave a 100% yield in 48 h, with an initial water activity of 0.11. Performance was poorer with direct addition of enzymatic preparation co-lyophilised with β-cyclodextrin and with addition of enzymatic preparation immobilized on a hydrophobic gel, with yields significantly less than 100%. We conclude that the lipolytic extract of P. corylophilum can be used in synthesis reactions in low-water systems, which justifies further efforts to purify lipolytic enzymes from the extract and to undertake an in-depth evaluation of their potential for use in biocatalysis, for the production of fine chemicals.Fil: Baron, Alessandra M.. Universidade Federal do Paraná; BrasilFil: Sarquis, María Inés M.. Colecâo de Cultura de Fungos; BrasilFil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Mitchell, David A.. Universidade Federal do Paraná; BrasilFil: Krieger, Nadia. Universidade Federal do Paraná; BrasilElsevier Science2005-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41516Baron, Alessandra M.; Sarquis, María Inés M.; Baigori, Mario Domingo; Mitchell, David A.; Krieger, Nadia; A comparative study of the synthesis of n-butyl-oleate using a crude lipolytic extract of Penicillum coryophilum in water-restricted environments; Elsevier Science; Journal of Molecular Catalysis B: Enzymatic; 34; 1-6; 7-2005; 25-321381-1177CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.molcatb.2005.04.002info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1381117705000433info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:47:14Zoai:ri.conicet.gov.ar:11336/41516instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:47:14.366CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A comparative study of the synthesis of n-butyl-oleate using a crude lipolytic extract of Penicillum coryophilum in water-restricted environments
title A comparative study of the synthesis of n-butyl-oleate using a crude lipolytic extract of Penicillum coryophilum in water-restricted environments
spellingShingle A comparative study of the synthesis of n-butyl-oleate using a crude lipolytic extract of Penicillum coryophilum in water-restricted environments
Baron, Alessandra M.
Lipases
Penicilium Corylophilum
Biocatalysis
Low-Water Systems
title_short A comparative study of the synthesis of n-butyl-oleate using a crude lipolytic extract of Penicillum coryophilum in water-restricted environments
title_full A comparative study of the synthesis of n-butyl-oleate using a crude lipolytic extract of Penicillum coryophilum in water-restricted environments
title_fullStr A comparative study of the synthesis of n-butyl-oleate using a crude lipolytic extract of Penicillum coryophilum in water-restricted environments
title_full_unstemmed A comparative study of the synthesis of n-butyl-oleate using a crude lipolytic extract of Penicillum coryophilum in water-restricted environments
title_sort A comparative study of the synthesis of n-butyl-oleate using a crude lipolytic extract of Penicillum coryophilum in water-restricted environments
dc.creator.none.fl_str_mv Baron, Alessandra M.
Sarquis, María Inés M.
Baigori, Mario Domingo
Mitchell, David A.
Krieger, Nadia
author Baron, Alessandra M.
author_facet Baron, Alessandra M.
Sarquis, María Inés M.
Baigori, Mario Domingo
Mitchell, David A.
Krieger, Nadia
author_role author
author2 Sarquis, María Inés M.
Baigori, Mario Domingo
Mitchell, David A.
Krieger, Nadia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Lipases
Penicilium Corylophilum
Biocatalysis
Low-Water Systems
topic Lipases
Penicilium Corylophilum
Biocatalysis
Low-Water Systems
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We evaluated the potential of a lipolytic extract from Penicillium corylophilum for use in biocatalysis. Lipolytic activity was stable when the extract was incubated in the presence of hydrophobic solvents. In fact, when the extract was incubated in n-heptane with an initial water activity of 0.95, the activity increased by 30%. Synthesis of n-butyl-oleate in different systems was compared. In AOT/n-heptane reverse micelles, a 100% yield of ester was obtained within 12 h, with a water content (W0, [H2O]/[AOT]) of 10. Several macro-heterogeneous systems involving n-heptane as the solvent were also compared. Direct addition of lyophilised enzymatic preparation to this system gave a 100% yield in 48 h, with an initial water activity of 0.11. Performance was poorer with direct addition of enzymatic preparation co-lyophilised with β-cyclodextrin and with addition of enzymatic preparation immobilized on a hydrophobic gel, with yields significantly less than 100%. We conclude that the lipolytic extract of P. corylophilum can be used in synthesis reactions in low-water systems, which justifies further efforts to purify lipolytic enzymes from the extract and to undertake an in-depth evaluation of their potential for use in biocatalysis, for the production of fine chemicals.
Fil: Baron, Alessandra M.. Universidade Federal do Paraná; Brasil
Fil: Sarquis, María Inés M.. Colecâo de Cultura de Fungos; Brasil
Fil: Baigori, Mario Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Mitchell, David A.. Universidade Federal do Paraná; Brasil
Fil: Krieger, Nadia. Universidade Federal do Paraná; Brasil
description We evaluated the potential of a lipolytic extract from Penicillium corylophilum for use in biocatalysis. Lipolytic activity was stable when the extract was incubated in the presence of hydrophobic solvents. In fact, when the extract was incubated in n-heptane with an initial water activity of 0.95, the activity increased by 30%. Synthesis of n-butyl-oleate in different systems was compared. In AOT/n-heptane reverse micelles, a 100% yield of ester was obtained within 12 h, with a water content (W0, [H2O]/[AOT]) of 10. Several macro-heterogeneous systems involving n-heptane as the solvent were also compared. Direct addition of lyophilised enzymatic preparation to this system gave a 100% yield in 48 h, with an initial water activity of 0.11. Performance was poorer with direct addition of enzymatic preparation co-lyophilised with β-cyclodextrin and with addition of enzymatic preparation immobilized on a hydrophobic gel, with yields significantly less than 100%. We conclude that the lipolytic extract of P. corylophilum can be used in synthesis reactions in low-water systems, which justifies further efforts to purify lipolytic enzymes from the extract and to undertake an in-depth evaluation of their potential for use in biocatalysis, for the production of fine chemicals.
publishDate 2005
dc.date.none.fl_str_mv 2005-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41516
Baron, Alessandra M.; Sarquis, María Inés M.; Baigori, Mario Domingo; Mitchell, David A.; Krieger, Nadia; A comparative study of the synthesis of n-butyl-oleate using a crude lipolytic extract of Penicillum coryophilum in water-restricted environments; Elsevier Science; Journal of Molecular Catalysis B: Enzymatic; 34; 1-6; 7-2005; 25-32
1381-1177
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41516
identifier_str_mv Baron, Alessandra M.; Sarquis, María Inés M.; Baigori, Mario Domingo; Mitchell, David A.; Krieger, Nadia; A comparative study of the synthesis of n-butyl-oleate using a crude lipolytic extract of Penicillum coryophilum in water-restricted environments; Elsevier Science; Journal of Molecular Catalysis B: Enzymatic; 34; 1-6; 7-2005; 25-32
1381-1177
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.molcatb.2005.04.002
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1381117705000433
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1848597935252570112
score 13.25334

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