Effect of functional groups on physicochemical and mechanical behavior of biocompatible macroporous hydrogels

Autores
Rivero, Rebeca Edith; Alustiza, Fabrisio Eduardo; Rodriguez, Nancy; Bosch, Pablo; Miras, Maria Cristina; Rivarola, Claudia Rosana; Barbero, César Alfredo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The increasing interest in studying the properties of biocompatible hydrogels is due to their possible applications in bioengineering. Properties of hydrogels based on N-isopropylacrylamide (NIPAM) and the effect caused by copolymerization with 2-acrylamido-2-methylpropanesulfonic acid (AMPS) or N-acryloyl-tris-(hydroxymethyl)aminomethane (HMA) were investigated. Hydrogels were synthesized by free radical polymerization at room temperature or by cryogelation at - 18°C. The presence of different functional groups (isopropyl, - SO3-, and -OH) and thermal conditions of polymerization affected the morphology and physicochemical and mechanical properties of hydrogels. Scanning electron microscopy (SEM) revealed the presence of macropores created by cryogelation with the morphology of the pores dependent on chemical composition of the copolymer. Poly(NIPAM-co-HMA) has spherical and isolated pores, whereas PNIPAM and Poly(NIPAM-co-AMPS) showed ellipsoidal interconnected pores. Three different water states were detected by differential scanning calorimetry (DSC), indicating the presence of nano- and macropores. Elastic modulus (E) was measured to be around 3-6.5 kPa by uniaxial compression. However, higher E values (20-30 kPa) and an anisotropic mechanical response were observed for PNIPAM and PNIPAM-co-AMPS hydrogels with ellipsoidal pores, a behavior that is almost similar to that of cartilage and bone tissues. Cytocompatibility studies using bovine fibroblasts (BFs) indicated good cell attachment and proliferation on PNIPAM-based hydrogel surfaces, although initially the cell adhesion varied depending on the composition of the surface. These hydrogels could be an interesting choice for the development of scaffolds in tissue engineering.
Fil: Rivero, Rebeca Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Alustiza, Fabrisio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Rodriguez, Nancy. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Bosch, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Miras, Maria Cristina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Rivarola, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Barbero, César Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Materia
Cryogelation
Fibroblasts
Macroporous Hydrogel
Mechanical Anisotropy
Water States
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/70839
Acceder
id CONICETDig_7f59ba4c75d0d2a73c4b40fe8c851a79
oai_identifier_str oai:ri.conicet.gov.ar:11336/70839
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of functional groups on physicochemical and mechanical behavior of biocompatible macroporous hydrogelsRivero, Rebeca EdithAlustiza, Fabrisio EduardoRodriguez, NancyBosch, PabloMiras, Maria CristinaRivarola, Claudia RosanaBarbero, César AlfredoCryogelationFibroblastsMacroporous HydrogelMechanical AnisotropyWater Stateshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The increasing interest in studying the properties of biocompatible hydrogels is due to their possible applications in bioengineering. Properties of hydrogels based on N-isopropylacrylamide (NIPAM) and the effect caused by copolymerization with 2-acrylamido-2-methylpropanesulfonic acid (AMPS) or N-acryloyl-tris-(hydroxymethyl)aminomethane (HMA) were investigated. Hydrogels were synthesized by free radical polymerization at room temperature or by cryogelation at - 18°C. The presence of different functional groups (isopropyl, - SO3-, and -OH) and thermal conditions of polymerization affected the morphology and physicochemical and mechanical properties of hydrogels. Scanning electron microscopy (SEM) revealed the presence of macropores created by cryogelation with the morphology of the pores dependent on chemical composition of the copolymer. Poly(NIPAM-co-HMA) has spherical and isolated pores, whereas PNIPAM and Poly(NIPAM-co-AMPS) showed ellipsoidal interconnected pores. Three different water states were detected by differential scanning calorimetry (DSC), indicating the presence of nano- and macropores. Elastic modulus (E) was measured to be around 3-6.5 kPa by uniaxial compression. However, higher E values (20-30 kPa) and an anisotropic mechanical response were observed for PNIPAM and PNIPAM-co-AMPS hydrogels with ellipsoidal pores, a behavior that is almost similar to that of cartilage and bone tissues. Cytocompatibility studies using bovine fibroblasts (BFs) indicated good cell attachment and proliferation on PNIPAM-based hydrogel surfaces, although initially the cell adhesion varied depending on the composition of the surface. These hydrogels could be an interesting choice for the development of scaffolds in tissue engineering.Fil: Rivero, Rebeca Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Alustiza, Fabrisio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Rodriguez, Nancy. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Bosch, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Miras, Maria Cristina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Rivarola, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Barbero, César Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaElsevier Science2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/70839Rivero, Rebeca Edith; Alustiza, Fabrisio Eduardo; Rodriguez, Nancy; Bosch, Pablo; Miras, Maria Cristina; et al.; Effect of functional groups on physicochemical and mechanical behavior of biocompatible macroporous hydrogels; Elsevier Science; Reactive & Functional Polymers; 97; 12-2015; 77-851381-5148CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.reactfunctpolym.2015.10.011info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1381514815300596info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:44Zoai:ri.conicet.gov.ar:11336/70839instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:45.125CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of functional groups on physicochemical and mechanical behavior of biocompatible macroporous hydrogels
title Effect of functional groups on physicochemical and mechanical behavior of biocompatible macroporous hydrogels
spellingShingle Effect of functional groups on physicochemical and mechanical behavior of biocompatible macroporous hydrogels
Rivero, Rebeca Edith
Cryogelation
Fibroblasts
Macroporous Hydrogel
Mechanical Anisotropy
Water States
title_short Effect of functional groups on physicochemical and mechanical behavior of biocompatible macroporous hydrogels
title_full Effect of functional groups on physicochemical and mechanical behavior of biocompatible macroporous hydrogels
title_fullStr Effect of functional groups on physicochemical and mechanical behavior of biocompatible macroporous hydrogels
title_full_unstemmed Effect of functional groups on physicochemical and mechanical behavior of biocompatible macroporous hydrogels
title_sort Effect of functional groups on physicochemical and mechanical behavior of biocompatible macroporous hydrogels
dc.creator.none.fl_str_mv Rivero, Rebeca Edith
Alustiza, Fabrisio Eduardo
Rodriguez, Nancy
Bosch, Pablo
Miras, Maria Cristina
Rivarola, Claudia Rosana
Barbero, César Alfredo
author Rivero, Rebeca Edith
author_facet Rivero, Rebeca Edith
Alustiza, Fabrisio Eduardo
Rodriguez, Nancy
Bosch, Pablo
Miras, Maria Cristina
Rivarola, Claudia Rosana
Barbero, César Alfredo
author_role author
author2 Alustiza, Fabrisio Eduardo
Rodriguez, Nancy
Bosch, Pablo
Miras, Maria Cristina
Rivarola, Claudia Rosana
Barbero, César Alfredo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cryogelation
Fibroblasts
Macroporous Hydrogel
Mechanical Anisotropy
Water States
topic Cryogelation
Fibroblasts
Macroporous Hydrogel
Mechanical Anisotropy
Water States
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The increasing interest in studying the properties of biocompatible hydrogels is due to their possible applications in bioengineering. Properties of hydrogels based on N-isopropylacrylamide (NIPAM) and the effect caused by copolymerization with 2-acrylamido-2-methylpropanesulfonic acid (AMPS) or N-acryloyl-tris-(hydroxymethyl)aminomethane (HMA) were investigated. Hydrogels were synthesized by free radical polymerization at room temperature or by cryogelation at - 18°C. The presence of different functional groups (isopropyl, - SO3-, and -OH) and thermal conditions of polymerization affected the morphology and physicochemical and mechanical properties of hydrogels. Scanning electron microscopy (SEM) revealed the presence of macropores created by cryogelation with the morphology of the pores dependent on chemical composition of the copolymer. Poly(NIPAM-co-HMA) has spherical and isolated pores, whereas PNIPAM and Poly(NIPAM-co-AMPS) showed ellipsoidal interconnected pores. Three different water states were detected by differential scanning calorimetry (DSC), indicating the presence of nano- and macropores. Elastic modulus (E) was measured to be around 3-6.5 kPa by uniaxial compression. However, higher E values (20-30 kPa) and an anisotropic mechanical response were observed for PNIPAM and PNIPAM-co-AMPS hydrogels with ellipsoidal pores, a behavior that is almost similar to that of cartilage and bone tissues. Cytocompatibility studies using bovine fibroblasts (BFs) indicated good cell attachment and proliferation on PNIPAM-based hydrogel surfaces, although initially the cell adhesion varied depending on the composition of the surface. These hydrogels could be an interesting choice for the development of scaffolds in tissue engineering.
Fil: Rivero, Rebeca Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Alustiza, Fabrisio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Rodriguez, Nancy. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Bosch, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Miras, Maria Cristina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Rivarola, Claudia Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Barbero, César Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
description The increasing interest in studying the properties of biocompatible hydrogels is due to their possible applications in bioengineering. Properties of hydrogels based on N-isopropylacrylamide (NIPAM) and the effect caused by copolymerization with 2-acrylamido-2-methylpropanesulfonic acid (AMPS) or N-acryloyl-tris-(hydroxymethyl)aminomethane (HMA) were investigated. Hydrogels were synthesized by free radical polymerization at room temperature or by cryogelation at - 18°C. The presence of different functional groups (isopropyl, - SO3-, and -OH) and thermal conditions of polymerization affected the morphology and physicochemical and mechanical properties of hydrogels. Scanning electron microscopy (SEM) revealed the presence of macropores created by cryogelation with the morphology of the pores dependent on chemical composition of the copolymer. Poly(NIPAM-co-HMA) has spherical and isolated pores, whereas PNIPAM and Poly(NIPAM-co-AMPS) showed ellipsoidal interconnected pores. Three different water states were detected by differential scanning calorimetry (DSC), indicating the presence of nano- and macropores. Elastic modulus (E) was measured to be around 3-6.5 kPa by uniaxial compression. However, higher E values (20-30 kPa) and an anisotropic mechanical response were observed for PNIPAM and PNIPAM-co-AMPS hydrogels with ellipsoidal pores, a behavior that is almost similar to that of cartilage and bone tissues. Cytocompatibility studies using bovine fibroblasts (BFs) indicated good cell attachment and proliferation on PNIPAM-based hydrogel surfaces, although initially the cell adhesion varied depending on the composition of the surface. These hydrogels could be an interesting choice for the development of scaffolds in tissue engineering.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/70839
Rivero, Rebeca Edith; Alustiza, Fabrisio Eduardo; Rodriguez, Nancy; Bosch, Pablo; Miras, Maria Cristina; et al.; Effect of functional groups on physicochemical and mechanical behavior of biocompatible macroporous hydrogels; Elsevier Science; Reactive & Functional Polymers; 97; 12-2015; 77-85
1381-5148
CONICET Digital
CONICET
url http://hdl.handle.net/11336/70839
identifier_str_mv Rivero, Rebeca Edith; Alustiza, Fabrisio Eduardo; Rodriguez, Nancy; Bosch, Pablo; Miras, Maria Cristina; et al.; Effect of functional groups on physicochemical and mechanical behavior of biocompatible macroporous hydrogels; Elsevier Science; Reactive & Functional Polymers; 97; 12-2015; 77-85
1381-5148
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.reactfunctpolym.2015.10.011
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1381514815300596
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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