Mechanical and physicochemical behavior of a 3D hydrogel scaffold during cell growth and proliferation

Autores
Rivero, Rebeca Edith; Capella, Virginia; Liaudat, Ana Cecilia; Bosch, Pablo; Barbero, César Alfredo; Rodriguez, Nancy; Rivarola, Claudia Rosana
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Some of the essential properties for cellular scaffolding are the capability to maintain the three-dimensional (3D) structure, good adhesion, and adequate elastic modulus during cell growth, migration, and proliferation. Biocompatible synthetic hydrogels are commonly used as cellular scaffolds because they can mimic the natural extracellular matrices (ECMs). However, it is possible that the physicochemical and mechanical behavior of the scaffold changes during cell proliferation and loses the scaffold properties but this is rarely monitored. In this work, the physicochemical and mechanical properties of a macroporous soft material based on poly(N-isopropyl acrylamide) (PNIPAM) have been studied during a period of 75 days at culture condition while bovine fetal fibroblasts (BFF) were grown within the matrix. The interconnected macroporous hydrogel was obtained by cryogelation at -18 °C. The swelling capacity of the scaffold was not altered during cell proliferation but changes in the mechanical properties were observed, beginning with the high elastic modulus (280 kPa) that progressively decreased until mechanical stability (40 kPa) was achieved after 20 culture days. It was observed that the matrix-cell interactions together with collagen production favor normal cellular processes such as cell morphology, adhesion, migration, and proliferation. Therefore, the observed behavior of macroporous PNIPAM as a 3D scaffold during cell growth indicates that the soft matrix is cytocompatible for a long time and preserves the suitable properties that can be applied in tissue engineering and regenerative medicine.
Fil: Rivero, Rebeca Edith. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Capella, Virginia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Liaudat, Ana Cecilia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Bosch, Pablo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Barbero, César Alfredo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Rodriguez, Nancy. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Rivarola, Claudia Rosana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Materia
HYDROGEL
PNIPAM
FIBROBLASTS
SCAFFOLD
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/145201
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/145201
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network_name_str CONICET Digital (CONICET)
spelling Mechanical and physicochemical behavior of a 3D hydrogel scaffold during cell growth and proliferationRivero, Rebeca EdithCapella, VirginiaLiaudat, Ana CeciliaBosch, PabloBarbero, César AlfredoRodriguez, NancyRivarola, Claudia RosanaHYDROGELPNIPAMFIBROBLASTSSCAFFOLDhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Some of the essential properties for cellular scaffolding are the capability to maintain the three-dimensional (3D) structure, good adhesion, and adequate elastic modulus during cell growth, migration, and proliferation. Biocompatible synthetic hydrogels are commonly used as cellular scaffolds because they can mimic the natural extracellular matrices (ECMs). However, it is possible that the physicochemical and mechanical behavior of the scaffold changes during cell proliferation and loses the scaffold properties but this is rarely monitored. In this work, the physicochemical and mechanical properties of a macroporous soft material based on poly(N-isopropyl acrylamide) (PNIPAM) have been studied during a period of 75 days at culture condition while bovine fetal fibroblasts (BFF) were grown within the matrix. The interconnected macroporous hydrogel was obtained by cryogelation at -18 °C. The swelling capacity of the scaffold was not altered during cell proliferation but changes in the mechanical properties were observed, beginning with the high elastic modulus (280 kPa) that progressively decreased until mechanical stability (40 kPa) was achieved after 20 culture days. It was observed that the matrix-cell interactions together with collagen production favor normal cellular processes such as cell morphology, adhesion, migration, and proliferation. Therefore, the observed behavior of macroporous PNIPAM as a 3D scaffold during cell growth indicates that the soft matrix is cytocompatible for a long time and preserves the suitable properties that can be applied in tissue engineering and regenerative medicine.Fil: Rivero, Rebeca Edith. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Capella, Virginia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Liaudat, Ana Cecilia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Bosch, Pablo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Barbero, César Alfredo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Rodriguez, Nancy. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Rivarola, Claudia Rosana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaRoyal Society of Chemistry2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/145201Rivero, Rebeca Edith; Capella, Virginia; Liaudat, Ana Cecilia; Bosch, Pablo; Barbero, César Alfredo; et al.; Mechanical and physicochemical behavior of a 3D hydrogel scaffold during cell growth and proliferation; Royal Society of Chemistry; RSC Advances; 10; 10; 2-2020; 5827-58372046-2069CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://xlink.rsc.org/?DOI=C9RA08162Cinfo:eu-repo/semantics/altIdentifier/doi/10.1039/C9RA08162Cinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:17Zoai:ri.conicet.gov.ar:11336/145201instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:18.112CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mechanical and physicochemical behavior of a 3D hydrogel scaffold during cell growth and proliferation
title Mechanical and physicochemical behavior of a 3D hydrogel scaffold during cell growth and proliferation
spellingShingle Mechanical and physicochemical behavior of a 3D hydrogel scaffold during cell growth and proliferation
Rivero, Rebeca Edith
HYDROGEL
PNIPAM
FIBROBLASTS
SCAFFOLD
title_short Mechanical and physicochemical behavior of a 3D hydrogel scaffold during cell growth and proliferation
title_full Mechanical and physicochemical behavior of a 3D hydrogel scaffold during cell growth and proliferation
title_fullStr Mechanical and physicochemical behavior of a 3D hydrogel scaffold during cell growth and proliferation
title_full_unstemmed Mechanical and physicochemical behavior of a 3D hydrogel scaffold during cell growth and proliferation
title_sort Mechanical and physicochemical behavior of a 3D hydrogel scaffold during cell growth and proliferation
dc.creator.none.fl_str_mv Rivero, Rebeca Edith
Capella, Virginia
Liaudat, Ana Cecilia
Bosch, Pablo
Barbero, César Alfredo
Rodriguez, Nancy
Rivarola, Claudia Rosana
author Rivero, Rebeca Edith
author_facet Rivero, Rebeca Edith
Capella, Virginia
Liaudat, Ana Cecilia
Bosch, Pablo
Barbero, César Alfredo
Rodriguez, Nancy
Rivarola, Claudia Rosana
author_role author
author2 Capella, Virginia
Liaudat, Ana Cecilia
Bosch, Pablo
Barbero, César Alfredo
Rodriguez, Nancy
Rivarola, Claudia Rosana
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv HYDROGEL
PNIPAM
FIBROBLASTS
SCAFFOLD
topic HYDROGEL
PNIPAM
FIBROBLASTS
SCAFFOLD
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Some of the essential properties for cellular scaffolding are the capability to maintain the three-dimensional (3D) structure, good adhesion, and adequate elastic modulus during cell growth, migration, and proliferation. Biocompatible synthetic hydrogels are commonly used as cellular scaffolds because they can mimic the natural extracellular matrices (ECMs). However, it is possible that the physicochemical and mechanical behavior of the scaffold changes during cell proliferation and loses the scaffold properties but this is rarely monitored. In this work, the physicochemical and mechanical properties of a macroporous soft material based on poly(N-isopropyl acrylamide) (PNIPAM) have been studied during a period of 75 days at culture condition while bovine fetal fibroblasts (BFF) were grown within the matrix. The interconnected macroporous hydrogel was obtained by cryogelation at -18 °C. The swelling capacity of the scaffold was not altered during cell proliferation but changes in the mechanical properties were observed, beginning with the high elastic modulus (280 kPa) that progressively decreased until mechanical stability (40 kPa) was achieved after 20 culture days. It was observed that the matrix-cell interactions together with collagen production favor normal cellular processes such as cell morphology, adhesion, migration, and proliferation. Therefore, the observed behavior of macroporous PNIPAM as a 3D scaffold during cell growth indicates that the soft matrix is cytocompatible for a long time and preserves the suitable properties that can be applied in tissue engineering and regenerative medicine.
Fil: Rivero, Rebeca Edith. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Capella, Virginia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Liaudat, Ana Cecilia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Bosch, Pablo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Barbero, César Alfredo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Rodriguez, Nancy. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Rivarola, Claudia Rosana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
description Some of the essential properties for cellular scaffolding are the capability to maintain the three-dimensional (3D) structure, good adhesion, and adequate elastic modulus during cell growth, migration, and proliferation. Biocompatible synthetic hydrogels are commonly used as cellular scaffolds because they can mimic the natural extracellular matrices (ECMs). However, it is possible that the physicochemical and mechanical behavior of the scaffold changes during cell proliferation and loses the scaffold properties but this is rarely monitored. In this work, the physicochemical and mechanical properties of a macroporous soft material based on poly(N-isopropyl acrylamide) (PNIPAM) have been studied during a period of 75 days at culture condition while bovine fetal fibroblasts (BFF) were grown within the matrix. The interconnected macroporous hydrogel was obtained by cryogelation at -18 °C. The swelling capacity of the scaffold was not altered during cell proliferation but changes in the mechanical properties were observed, beginning with the high elastic modulus (280 kPa) that progressively decreased until mechanical stability (40 kPa) was achieved after 20 culture days. It was observed that the matrix-cell interactions together with collagen production favor normal cellular processes such as cell morphology, adhesion, migration, and proliferation. Therefore, the observed behavior of macroporous PNIPAM as a 3D scaffold during cell growth indicates that the soft matrix is cytocompatible for a long time and preserves the suitable properties that can be applied in tissue engineering and regenerative medicine.
publishDate 2020
dc.date.none.fl_str_mv 2020-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/145201
Rivero, Rebeca Edith; Capella, Virginia; Liaudat, Ana Cecilia; Bosch, Pablo; Barbero, César Alfredo; et al.; Mechanical and physicochemical behavior of a 3D hydrogel scaffold during cell growth and proliferation; Royal Society of Chemistry; RSC Advances; 10; 10; 2-2020; 5827-5837
2046-2069
CONICET Digital
CONICET
url http://hdl.handle.net/11336/145201
identifier_str_mv Rivero, Rebeca Edith; Capella, Virginia; Liaudat, Ana Cecilia; Bosch, Pablo; Barbero, César Alfredo; et al.; Mechanical and physicochemical behavior of a 3D hydrogel scaffold during cell growth and proliferation; Royal Society of Chemistry; RSC Advances; 10; 10; 2-2020; 5827-5837
2046-2069
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://xlink.rsc.org/?DOI=C9RA08162C
info:eu-repo/semantics/altIdentifier/doi/10.1039/C9RA08162C
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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