Immobilization of lipase from Candida rugosa on synthesized hydrogel for hydrolysis reaction

Autores
Garcia Manzano, Maria Florencia; Alvarez Igarzabal, Cecilia Ines
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Novel hydrogels based on N-acryloyl-tris(hydroxymethyl) aminomethane (NAT), 2-aminoethyl methacrylate (AEMA) and N,N-methylenebisacrylamide (BIS) were prepared and applied for immobilization of lipase. Two procedures were previously performed on the hydrogels as follows: (a) modification with epichlorohydrin (ECH) or 1,4-butanediol diglycidyl ether (BDGE) or (b) grafting with AEMA, using ceric ammonium sulfate as an initiator, and subsequent reaction with ECH. In the hydrolysis reactions, high percentages of lipase activity were reached with products whose enzyme immobilization were performed on both BDGE-containing products and grafted matrices, since the enzyme was bound spaced from the matrices and more likely to interact with the substrate. The best derivatives preserved 86.7?89.5% of the activity corresponding to the soluble enzyme.
Fil: Garcia Manzano, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina
Fil: Alvarez Igarzabal, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina
Materia
Hydrogel
Modification
Ommobilization
Candida Rugosa Lipase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13020
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/13020
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immobilization of lipase from Candida rugosa on synthesized hydrogel for hydrolysis reactionGarcia Manzano, Maria FlorenciaAlvarez Igarzabal, Cecilia InesHydrogelModificationOmmobilizationCandida Rugosa Lipasehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Novel hydrogels based on N-acryloyl-tris(hydroxymethyl) aminomethane (NAT), 2-aminoethyl methacrylate (AEMA) and N,N-methylenebisacrylamide (BIS) were prepared and applied for immobilization of lipase. Two procedures were previously performed on the hydrogels as follows: (a) modification with epichlorohydrin (ECH) or 1,4-butanediol diglycidyl ether (BDGE) or (b) grafting with AEMA, using ceric ammonium sulfate as an initiator, and subsequent reaction with ECH. In the hydrolysis reactions, high percentages of lipase activity were reached with products whose enzyme immobilization were performed on both BDGE-containing products and grafted matrices, since the enzyme was bound spaced from the matrices and more likely to interact with the substrate. The best derivatives preserved 86.7?89.5% of the activity corresponding to the soluble enzyme.Fil: Garcia Manzano, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; ArgentinaFil: Alvarez Igarzabal, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; ArgentinaElsevier Science2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13020Garcia Manzano, Maria Florencia; Alvarez Igarzabal, Cecilia Ines; Immobilization of lipase from Candida rugosa on synthesized hydrogel for hydrolysis reaction; Elsevier Science; Journal Of Molecular Catalysis B: Enzymatic; 72; 1-2; 4-2011; 28-351381-11771873-3158enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S138111771100124Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.molcatb.2011.04.020info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:39Zoai:ri.conicet.gov.ar:11336/13020instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:39.959CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immobilization of lipase from Candida rugosa on synthesized hydrogel for hydrolysis reaction
title Immobilization of lipase from Candida rugosa on synthesized hydrogel for hydrolysis reaction
spellingShingle Immobilization of lipase from Candida rugosa on synthesized hydrogel for hydrolysis reaction
Garcia Manzano, Maria Florencia
Hydrogel
Modification
Ommobilization
Candida Rugosa Lipase
title_short Immobilization of lipase from Candida rugosa on synthesized hydrogel for hydrolysis reaction
title_full Immobilization of lipase from Candida rugosa on synthesized hydrogel for hydrolysis reaction
title_fullStr Immobilization of lipase from Candida rugosa on synthesized hydrogel for hydrolysis reaction
title_full_unstemmed Immobilization of lipase from Candida rugosa on synthesized hydrogel for hydrolysis reaction
title_sort Immobilization of lipase from Candida rugosa on synthesized hydrogel for hydrolysis reaction
dc.creator.none.fl_str_mv Garcia Manzano, Maria Florencia
Alvarez Igarzabal, Cecilia Ines
author Garcia Manzano, Maria Florencia
author_facet Garcia Manzano, Maria Florencia
Alvarez Igarzabal, Cecilia Ines
author_role author
author2 Alvarez Igarzabal, Cecilia Ines
author2_role author
dc.subject.none.fl_str_mv Hydrogel
Modification
Ommobilization
Candida Rugosa Lipase
topic Hydrogel
Modification
Ommobilization
Candida Rugosa Lipase
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Novel hydrogels based on N-acryloyl-tris(hydroxymethyl) aminomethane (NAT), 2-aminoethyl methacrylate (AEMA) and N,N-methylenebisacrylamide (BIS) were prepared and applied for immobilization of lipase. Two procedures were previously performed on the hydrogels as follows: (a) modification with epichlorohydrin (ECH) or 1,4-butanediol diglycidyl ether (BDGE) or (b) grafting with AEMA, using ceric ammonium sulfate as an initiator, and subsequent reaction with ECH. In the hydrolysis reactions, high percentages of lipase activity were reached with products whose enzyme immobilization were performed on both BDGE-containing products and grafted matrices, since the enzyme was bound spaced from the matrices and more likely to interact with the substrate. The best derivatives preserved 86.7?89.5% of the activity corresponding to the soluble enzyme.
Fil: Garcia Manzano, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina
Fil: Alvarez Igarzabal, Cecilia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina
description Novel hydrogels based on N-acryloyl-tris(hydroxymethyl) aminomethane (NAT), 2-aminoethyl methacrylate (AEMA) and N,N-methylenebisacrylamide (BIS) were prepared and applied for immobilization of lipase. Two procedures were previously performed on the hydrogels as follows: (a) modification with epichlorohydrin (ECH) or 1,4-butanediol diglycidyl ether (BDGE) or (b) grafting with AEMA, using ceric ammonium sulfate as an initiator, and subsequent reaction with ECH. In the hydrolysis reactions, high percentages of lipase activity were reached with products whose enzyme immobilization were performed on both BDGE-containing products and grafted matrices, since the enzyme was bound spaced from the matrices and more likely to interact with the substrate. The best derivatives preserved 86.7?89.5% of the activity corresponding to the soluble enzyme.
publishDate 2011
dc.date.none.fl_str_mv 2011-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13020
Garcia Manzano, Maria Florencia; Alvarez Igarzabal, Cecilia Ines; Immobilization of lipase from Candida rugosa on synthesized hydrogel for hydrolysis reaction; Elsevier Science; Journal Of Molecular Catalysis B: Enzymatic; 72; 1-2; 4-2011; 28-35
1381-1177
1873-3158
url http://hdl.handle.net/11336/13020
identifier_str_mv Garcia Manzano, Maria Florencia; Alvarez Igarzabal, Cecilia Ines; Immobilization of lipase from Candida rugosa on synthesized hydrogel for hydrolysis reaction; Elsevier Science; Journal Of Molecular Catalysis B: Enzymatic; 72; 1-2; 4-2011; 28-35
1381-1177
1873-3158
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S138111771100124X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.molcatb.2011.04.020
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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