Immobilization of gold nanoparticles on living cell membranes upon controlled lipid binding

Autores
Ba, Haojin; Rodríguez Fernández, Jessica; Stefani, Fernando Daniel; Feldmann, Jochen
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We present a versatile and controlled route to immobilize gold nanoparticles (NPs) on the surface of living cells, while preserving the sensing and optothermal capabilities of the original colloid. Our approach is based on the controlled and selective binding of Au NPs to phospholipids prior to cell incubation. We show that in the presence of the cells the lipid-bound Au NPs are delivered to the cellular membrane and that their diffusion is rather slow and spatially limited, as a result of lipid binding. Avoiding nonspecific membrane labeling, this approach is of general application to several types of colloids and cells and thereby provides a platform for controlled plasmonic and optothermal investigations of living cell membranes.
Fil: Ba, Haojin. Ludwig Maximilians Universitat; Alemania
Fil: Rodríguez Fernández, Jessica. Ludwig Maximilians Universitat; Alemania
Fil: Stefani, Fernando Daniel. Ludwig Maximilians Universitat; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Feldmann, Jochen. Ludwig Maximilians Universitat; Alemania
Materia
Cell Membrane
Diffusion
Gold Nanoparticles
Labeling
Lipid Binding
Surface Modification
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/68928
Acceder
id CONICETDig_893b354bf256015e278c0d2d7eae37cb
oai_identifier_str oai:ri.conicet.gov.ar:11336/68928
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immobilization of gold nanoparticles on living cell membranes upon controlled lipid bindingBa, HaojinRodríguez Fernández, JessicaStefani, Fernando DanielFeldmann, JochenCell MembraneDiffusionGold NanoparticlesLabelingLipid BindingSurface Modificationhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We present a versatile and controlled route to immobilize gold nanoparticles (NPs) on the surface of living cells, while preserving the sensing and optothermal capabilities of the original colloid. Our approach is based on the controlled and selective binding of Au NPs to phospholipids prior to cell incubation. We show that in the presence of the cells the lipid-bound Au NPs are delivered to the cellular membrane and that their diffusion is rather slow and spatially limited, as a result of lipid binding. Avoiding nonspecific membrane labeling, this approach is of general application to several types of colloids and cells and thereby provides a platform for controlled plasmonic and optothermal investigations of living cell membranes.Fil: Ba, Haojin. Ludwig Maximilians Universitat; AlemaniaFil: Rodríguez Fernández, Jessica. Ludwig Maximilians Universitat; AlemaniaFil: Stefani, Fernando Daniel. Ludwig Maximilians Universitat; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Feldmann, Jochen. Ludwig Maximilians Universitat; AlemaniaAmerican Chemical Society2010-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/68928Ba, Haojin; Rodríguez Fernández, Jessica; Stefani, Fernando Daniel; Feldmann, Jochen; Immobilization of gold nanoparticles on living cell membranes upon controlled lipid binding; American Chemical Society; Nano Letters; 10; 8; 8-2010; 3006-30121530-6984CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/nl101454ainfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/nl101454ainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:03:58Zoai:ri.conicet.gov.ar:11336/68928instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:03:59.192CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immobilization of gold nanoparticles on living cell membranes upon controlled lipid binding
title Immobilization of gold nanoparticles on living cell membranes upon controlled lipid binding
spellingShingle Immobilization of gold nanoparticles on living cell membranes upon controlled lipid binding
Ba, Haojin
Cell Membrane
Diffusion
Gold Nanoparticles
Labeling
Lipid Binding
Surface Modification
title_short Immobilization of gold nanoparticles on living cell membranes upon controlled lipid binding
title_full Immobilization of gold nanoparticles on living cell membranes upon controlled lipid binding
title_fullStr Immobilization of gold nanoparticles on living cell membranes upon controlled lipid binding
title_full_unstemmed Immobilization of gold nanoparticles on living cell membranes upon controlled lipid binding
title_sort Immobilization of gold nanoparticles on living cell membranes upon controlled lipid binding
dc.creator.none.fl_str_mv Ba, Haojin
Rodríguez Fernández, Jessica
Stefani, Fernando Daniel
Feldmann, Jochen
author Ba, Haojin
author_facet Ba, Haojin
Rodríguez Fernández, Jessica
Stefani, Fernando Daniel
Feldmann, Jochen
author_role author
author2 Rodríguez Fernández, Jessica
Stefani, Fernando Daniel
Feldmann, Jochen
author2_role author
author
author
dc.subject.none.fl_str_mv Cell Membrane
Diffusion
Gold Nanoparticles
Labeling
Lipid Binding
Surface Modification
topic Cell Membrane
Diffusion
Gold Nanoparticles
Labeling
Lipid Binding
Surface Modification
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We present a versatile and controlled route to immobilize gold nanoparticles (NPs) on the surface of living cells, while preserving the sensing and optothermal capabilities of the original colloid. Our approach is based on the controlled and selective binding of Au NPs to phospholipids prior to cell incubation. We show that in the presence of the cells the lipid-bound Au NPs are delivered to the cellular membrane and that their diffusion is rather slow and spatially limited, as a result of lipid binding. Avoiding nonspecific membrane labeling, this approach is of general application to several types of colloids and cells and thereby provides a platform for controlled plasmonic and optothermal investigations of living cell membranes.
Fil: Ba, Haojin. Ludwig Maximilians Universitat; Alemania
Fil: Rodríguez Fernández, Jessica. Ludwig Maximilians Universitat; Alemania
Fil: Stefani, Fernando Daniel. Ludwig Maximilians Universitat; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Feldmann, Jochen. Ludwig Maximilians Universitat; Alemania
description We present a versatile and controlled route to immobilize gold nanoparticles (NPs) on the surface of living cells, while preserving the sensing and optothermal capabilities of the original colloid. Our approach is based on the controlled and selective binding of Au NPs to phospholipids prior to cell incubation. We show that in the presence of the cells the lipid-bound Au NPs are delivered to the cellular membrane and that their diffusion is rather slow and spatially limited, as a result of lipid binding. Avoiding nonspecific membrane labeling, this approach is of general application to several types of colloids and cells and thereby provides a platform for controlled plasmonic and optothermal investigations of living cell membranes.
publishDate 2010
dc.date.none.fl_str_mv 2010-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/68928
Ba, Haojin; Rodríguez Fernández, Jessica; Stefani, Fernando Daniel; Feldmann, Jochen; Immobilization of gold nanoparticles on living cell membranes upon controlled lipid binding; American Chemical Society; Nano Letters; 10; 8; 8-2010; 3006-3012
1530-6984
CONICET Digital
CONICET
url http://hdl.handle.net/11336/68928
identifier_str_mv Ba, Haojin; Rodríguez Fernández, Jessica; Stefani, Fernando Daniel; Feldmann, Jochen; Immobilization of gold nanoparticles on living cell membranes upon controlled lipid binding; American Chemical Society; Nano Letters; 10; 8; 8-2010; 3006-3012
1530-6984
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/nl101454a
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/nl101454a
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613862052069376
score 13.070432

Publicaciones similares