Generation of a genomic tiling array of the human Major Histocompatibility Complex (MHC) and its application for DNA methylation analysis
- Autores
- Tomazou, Eleni M; Rakyan, Vardhman K; Lefebvre, Gregory; Andrews, Robert; Ellis, Peter; Jackson, David K; Langford, Cordelia; Francis, Matthew D; Bäckdahl, Liselotte; Miretti, Marcos Mateo; Coggill, Penny; Ottaviani, Diego; Sheer, Denise; Murrell, Adele; Beck, Stephan G.
- Año de publicación
- 2008
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: The major histocompatibility complex (MHC) is essential for human immunity and is highly associated with common diseases, including cancer. While the genetics of the MHC has been studied intensively for many decades, very little is known about the epigenetics of this most polymorphic and disease-associated region of the genome. Methods: To facilitate comprehensive epigenetic analyses of this region, we have generated a genomic tiling array of 2 Kb resolution covering the entire 4 Mb MHC region. The array has been designed to be compatible with chromatin immunoprecipitation (ChIP), methylated DNA immunoprecipitation (MeDIP), array comparative genomic hybridization (aCGH) and expression profiling, including of non-coding RNAs. The array comprises 7832 features, consisting of two replicates of both forward and reverse strands of MHC amplicons and appropriate controls. Results: Using MeDIP, we demonstrate the application of the MHC array for DNA methylation profiling and the identification of tissue-specific differentially methylated regions (tDMRs). Based on the analysis of two tissues and two cell types, we identified 90 tDMRs within the MHC and describe their characterisation. Conclusion: A tiling array covering the MHC region was developed and validated. Its successful application for DNA methylation profiling indicates that this array represents a useful tool for molecular analyses of the MHC in the context of medical genomics.
Fil: Tomazou, Eleni M. Wellcome Trust Sanger Institute; Reino Unido
Fil: Rakyan, Vardhman K. Institute of Cell and Molecular Science; Reino Unido
Fil: Lefebvre, Gregory. Wellcome Trust Sanger Institute; Reino Unido
Fil: Andrews, Robert. Wellcome Trust Sanger Institute; Reino Unido
Fil: Ellis, Peter. Wellcome Trust Sanger Institute; Reino Unido
Fil: Jackson, David K. Wellcome Trust Sanger Institute; Reino Unido
Fil: Langford, Cordelia. Wellcome Trust Sanger Institute; Reino Unido
Fil: Francis, Matthew D. Wellcome Trust Sanger Institute; Reino Unido
Fil: Bäckdahl, Liselotte. University College London; Reino Unido
Fil: Miretti, Marcos Mateo. Wellcome Trust Sanger Institute; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Coggill, Penny. Wellcome Trust Sanger Institute; Reino Unido
Fil: Ottaviani, Diego. London Research Institute; Reino Unido. London School of Medicine and Dentistry; Reino Unido
Fil: Sheer, Denise. London School of Medicine and Dentistry; Reino Unido
Fil: Murrell, Adele. Cambridge Research Institute; Reino Unido
Fil: Beck, Stephan G.. University College London; Reino Unido - Materia
-
HUMAN MAJOR HISTOCOMPATIBILITY
HLA
GENOMIC ARRAY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/113919
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/113919 |
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3498 |
network_name_str |
CONICET Digital (CONICET) |
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Generation of a genomic tiling array of the human Major Histocompatibility Complex (MHC) and its application for DNA methylation analysisTomazou, Eleni MRakyan, Vardhman KLefebvre, GregoryAndrews, RobertEllis, PeterJackson, David KLangford, CordeliaFrancis, Matthew DBäckdahl, LiselotteMiretti, Marcos MateoCoggill, PennyOttaviani, DiegoSheer, DeniseMurrell, AdeleBeck, Stephan G.HUMAN MAJOR HISTOCOMPATIBILITYHLAGENOMIC ARRAYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: The major histocompatibility complex (MHC) is essential for human immunity and is highly associated with common diseases, including cancer. While the genetics of the MHC has been studied intensively for many decades, very little is known about the epigenetics of this most polymorphic and disease-associated region of the genome. Methods: To facilitate comprehensive epigenetic analyses of this region, we have generated a genomic tiling array of 2 Kb resolution covering the entire 4 Mb MHC region. The array has been designed to be compatible with chromatin immunoprecipitation (ChIP), methylated DNA immunoprecipitation (MeDIP), array comparative genomic hybridization (aCGH) and expression profiling, including of non-coding RNAs. The array comprises 7832 features, consisting of two replicates of both forward and reverse strands of MHC amplicons and appropriate controls. Results: Using MeDIP, we demonstrate the application of the MHC array for DNA methylation profiling and the identification of tissue-specific differentially methylated regions (tDMRs). Based on the analysis of two tissues and two cell types, we identified 90 tDMRs within the MHC and describe their characterisation. Conclusion: A tiling array covering the MHC region was developed and validated. Its successful application for DNA methylation profiling indicates that this array represents a useful tool for molecular analyses of the MHC in the context of medical genomics.Fil: Tomazou, Eleni M. Wellcome Trust Sanger Institute; Reino UnidoFil: Rakyan, Vardhman K. Institute of Cell and Molecular Science; Reino UnidoFil: Lefebvre, Gregory. Wellcome Trust Sanger Institute; Reino UnidoFil: Andrews, Robert. Wellcome Trust Sanger Institute; Reino UnidoFil: Ellis, Peter. Wellcome Trust Sanger Institute; Reino UnidoFil: Jackson, David K. Wellcome Trust Sanger Institute; Reino UnidoFil: Langford, Cordelia. Wellcome Trust Sanger Institute; Reino UnidoFil: Francis, Matthew D. Wellcome Trust Sanger Institute; Reino UnidoFil: Bäckdahl, Liselotte. University College London; Reino UnidoFil: Miretti, Marcos Mateo. Wellcome Trust Sanger Institute; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Coggill, Penny. Wellcome Trust Sanger Institute; Reino UnidoFil: Ottaviani, Diego. London Research Institute; Reino Unido. London School of Medicine and Dentistry; Reino UnidoFil: Sheer, Denise. London School of Medicine and Dentistry; Reino UnidoFil: Murrell, Adele. Cambridge Research Institute; Reino UnidoFil: Beck, Stephan G.. University College London; Reino UnidoBioMed Central2008-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113919Tomazou, Eleni M; Rakyan, Vardhman K; Lefebvre, Gregory; Andrews, Robert; Ellis, Peter; et al.; Generation of a genomic tiling array of the human Major Histocompatibility Complex (MHC) and its application for DNA methylation analysis; BioMed Central; Bmc Medical Genomics; 1; 19; 5-2008; 1-161755-8794CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/1755-8794-1-19info:eu-repo/semantics/altIdentifier/url/https://bmcmedgenomics.biomedcentral.com/articles/10.1186/1755-8794-1-19info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:19Zoai:ri.conicet.gov.ar:11336/113919instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:19.617CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Generation of a genomic tiling array of the human Major Histocompatibility Complex (MHC) and its application for DNA methylation analysis |
title |
Generation of a genomic tiling array of the human Major Histocompatibility Complex (MHC) and its application for DNA methylation analysis |
spellingShingle |
Generation of a genomic tiling array of the human Major Histocompatibility Complex (MHC) and its application for DNA methylation analysis Tomazou, Eleni M HUMAN MAJOR HISTOCOMPATIBILITY HLA GENOMIC ARRAY |
title_short |
Generation of a genomic tiling array of the human Major Histocompatibility Complex (MHC) and its application for DNA methylation analysis |
title_full |
Generation of a genomic tiling array of the human Major Histocompatibility Complex (MHC) and its application for DNA methylation analysis |
title_fullStr |
Generation of a genomic tiling array of the human Major Histocompatibility Complex (MHC) and its application for DNA methylation analysis |
title_full_unstemmed |
Generation of a genomic tiling array of the human Major Histocompatibility Complex (MHC) and its application for DNA methylation analysis |
title_sort |
Generation of a genomic tiling array of the human Major Histocompatibility Complex (MHC) and its application for DNA methylation analysis |
dc.creator.none.fl_str_mv |
Tomazou, Eleni M Rakyan, Vardhman K Lefebvre, Gregory Andrews, Robert Ellis, Peter Jackson, David K Langford, Cordelia Francis, Matthew D Bäckdahl, Liselotte Miretti, Marcos Mateo Coggill, Penny Ottaviani, Diego Sheer, Denise Murrell, Adele Beck, Stephan G. |
author |
Tomazou, Eleni M |
author_facet |
Tomazou, Eleni M Rakyan, Vardhman K Lefebvre, Gregory Andrews, Robert Ellis, Peter Jackson, David K Langford, Cordelia Francis, Matthew D Bäckdahl, Liselotte Miretti, Marcos Mateo Coggill, Penny Ottaviani, Diego Sheer, Denise Murrell, Adele Beck, Stephan G. |
author_role |
author |
author2 |
Rakyan, Vardhman K Lefebvre, Gregory Andrews, Robert Ellis, Peter Jackson, David K Langford, Cordelia Francis, Matthew D Bäckdahl, Liselotte Miretti, Marcos Mateo Coggill, Penny Ottaviani, Diego Sheer, Denise Murrell, Adele Beck, Stephan G. |
author2_role |
author author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
HUMAN MAJOR HISTOCOMPATIBILITY HLA GENOMIC ARRAY |
topic |
HUMAN MAJOR HISTOCOMPATIBILITY HLA GENOMIC ARRAY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Background: The major histocompatibility complex (MHC) is essential for human immunity and is highly associated with common diseases, including cancer. While the genetics of the MHC has been studied intensively for many decades, very little is known about the epigenetics of this most polymorphic and disease-associated region of the genome. Methods: To facilitate comprehensive epigenetic analyses of this region, we have generated a genomic tiling array of 2 Kb resolution covering the entire 4 Mb MHC region. The array has been designed to be compatible with chromatin immunoprecipitation (ChIP), methylated DNA immunoprecipitation (MeDIP), array comparative genomic hybridization (aCGH) and expression profiling, including of non-coding RNAs. The array comprises 7832 features, consisting of two replicates of both forward and reverse strands of MHC amplicons and appropriate controls. Results: Using MeDIP, we demonstrate the application of the MHC array for DNA methylation profiling and the identification of tissue-specific differentially methylated regions (tDMRs). Based on the analysis of two tissues and two cell types, we identified 90 tDMRs within the MHC and describe their characterisation. Conclusion: A tiling array covering the MHC region was developed and validated. Its successful application for DNA methylation profiling indicates that this array represents a useful tool for molecular analyses of the MHC in the context of medical genomics. Fil: Tomazou, Eleni M. Wellcome Trust Sanger Institute; Reino Unido Fil: Rakyan, Vardhman K. Institute of Cell and Molecular Science; Reino Unido Fil: Lefebvre, Gregory. Wellcome Trust Sanger Institute; Reino Unido Fil: Andrews, Robert. Wellcome Trust Sanger Institute; Reino Unido Fil: Ellis, Peter. Wellcome Trust Sanger Institute; Reino Unido Fil: Jackson, David K. Wellcome Trust Sanger Institute; Reino Unido Fil: Langford, Cordelia. Wellcome Trust Sanger Institute; Reino Unido Fil: Francis, Matthew D. Wellcome Trust Sanger Institute; Reino Unido Fil: Bäckdahl, Liselotte. University College London; Reino Unido Fil: Miretti, Marcos Mateo. Wellcome Trust Sanger Institute; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina Fil: Coggill, Penny. Wellcome Trust Sanger Institute; Reino Unido Fil: Ottaviani, Diego. London Research Institute; Reino Unido. London School of Medicine and Dentistry; Reino Unido Fil: Sheer, Denise. London School of Medicine and Dentistry; Reino Unido Fil: Murrell, Adele. Cambridge Research Institute; Reino Unido Fil: Beck, Stephan G.. University College London; Reino Unido |
description |
Background: The major histocompatibility complex (MHC) is essential for human immunity and is highly associated with common diseases, including cancer. While the genetics of the MHC has been studied intensively for many decades, very little is known about the epigenetics of this most polymorphic and disease-associated region of the genome. Methods: To facilitate comprehensive epigenetic analyses of this region, we have generated a genomic tiling array of 2 Kb resolution covering the entire 4 Mb MHC region. The array has been designed to be compatible with chromatin immunoprecipitation (ChIP), methylated DNA immunoprecipitation (MeDIP), array comparative genomic hybridization (aCGH) and expression profiling, including of non-coding RNAs. The array comprises 7832 features, consisting of two replicates of both forward and reverse strands of MHC amplicons and appropriate controls. Results: Using MeDIP, we demonstrate the application of the MHC array for DNA methylation profiling and the identification of tissue-specific differentially methylated regions (tDMRs). Based on the analysis of two tissues and two cell types, we identified 90 tDMRs within the MHC and describe their characterisation. Conclusion: A tiling array covering the MHC region was developed and validated. Its successful application for DNA methylation profiling indicates that this array represents a useful tool for molecular analyses of the MHC in the context of medical genomics. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/113919 Tomazou, Eleni M; Rakyan, Vardhman K; Lefebvre, Gregory; Andrews, Robert; Ellis, Peter; et al.; Generation of a genomic tiling array of the human Major Histocompatibility Complex (MHC) and its application for DNA methylation analysis; BioMed Central; Bmc Medical Genomics; 1; 19; 5-2008; 1-16 1755-8794 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/113919 |
identifier_str_mv |
Tomazou, Eleni M; Rakyan, Vardhman K; Lefebvre, Gregory; Andrews, Robert; Ellis, Peter; et al.; Generation of a genomic tiling array of the human Major Histocompatibility Complex (MHC) and its application for DNA methylation analysis; BioMed Central; Bmc Medical Genomics; 1; 19; 5-2008; 1-16 1755-8794 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1186/1755-8794-1-19 info:eu-repo/semantics/altIdentifier/url/https://bmcmedgenomics.biomedcentral.com/articles/10.1186/1755-8794-1-19 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
BioMed Central |
publisher.none.fl_str_mv |
BioMed Central |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269953449787392 |
score |
13.13397 |