Early events following bovine leukaemia virus infection in calves with different alleles of the major histocompatibility complex  DRB3 gene

Autores
Forletti, Agustina; Lützelschwab, Claudia María; Cepeda, Rosana Esther; Esteban, Eduardo Néstor; Gutiérrez, Silvina Elena
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cattle maintaining a low proviral load (LPL) status after Bovine Leukemia Virus (BLV) infection have been recognized as BLV controllers, and non-transmitters to uninfected cattle in experimental and natural conditions. LPL has been associated with host genetics, mainly with BoLAclass II DRB3 gene. The aim of this work was to study the kinetics of the BLV and the host response in Holstein calves carrying different BoLA-DRB3 alleles. Twenty BLV free calves were inoculated with infected lymphocytes. Two calves were maintained uninfected as controls. Proviral load, total leukocyte and lymphocyte counts, anti-BLVgp51 titers and BLVp24 expression were determined in blood samples at various times post inoculation (dpi).Proviral loadpeaked at 30 dpi in all animals. It declined steadily from seroconversion (38 dpi) to the end of the study (178 dpi) in calves carrying resistance-associated allele (*0902), while it was maintained at elevated levels in calves with *1501 or neutral alleles after seroconversion. Leukocyte and lymphocyte counts and BLVp24 expression did not significantly differ between genetic groups. Animals with <20 proviral copies/30 ng DNA at 178 dpi or < 200 proviral copies at 88 dpi were classified as LPL, while calves with levels above these limits were considered as high proviral load (HPL) profile. All 6 calves with*1501 allele progressed to HPL, while LPL was attained by 6/7 (86%) and 2/6 (33%) of the calves with *0902 and neutral alleles respectively. One calf with both *0902 and *1501 developed LPL. This is the first experimental induction of LPL profile in cattle.
Fil: Forletti, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lützelschwab, Claudia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Cepeda, Rosana Esther. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Exactas. Instituto Multidisciplinario de Ecosistemas y Desarrollo Sustentable; Argentina
Fil: Esteban, Eduardo Néstor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Gutiérrez, Silvina Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Materia
BOVINE LEUKEMIA VIRUS
PROVIRAL LOAD
GENETIC RESISTANCE
BOLA-DRB3
MAJOR HISTOCOMPATIBILITY COMPLEX
EXPERIMENTAL INFECTION
QPCR
BLVP24
BLVGP51
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/257293
Acceder
id CONICETDig_5aaaf9758c5c2f318e5a02b2f00b6d77
oai_identifier_str oai:ri.conicet.gov.ar:11336/257293
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Early events following bovine leukaemia virus infection in calves with different alleles of the major histocompatibility complex  DRB3 geneForletti, AgustinaLützelschwab, Claudia MaríaCepeda, Rosana EstherEsteban, Eduardo NéstorGutiérrez, Silvina ElenaBOVINE LEUKEMIA VIRUSPROVIRAL LOADGENETIC RESISTANCEBOLA-DRB3MAJOR HISTOCOMPATIBILITY COMPLEXEXPERIMENTAL INFECTIONQPCRBLVP24BLVGP51https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Cattle maintaining a low proviral load (LPL) status after Bovine Leukemia Virus (BLV) infection have been recognized as BLV controllers, and non-transmitters to uninfected cattle in experimental and natural conditions. LPL has been associated with host genetics, mainly with BoLAclass II DRB3 gene. The aim of this work was to study the kinetics of the BLV and the host response in Holstein calves carrying different BoLA-DRB3 alleles. Twenty BLV free calves were inoculated with infected lymphocytes. Two calves were maintained uninfected as controls. Proviral load, total leukocyte and lymphocyte counts, anti-BLVgp51 titers and BLVp24 expression were determined in blood samples at various times post inoculation (dpi).Proviral loadpeaked at 30 dpi in all animals. It declined steadily from seroconversion (38 dpi) to the end of the study (178 dpi) in calves carrying resistance-associated allele (*0902), while it was maintained at elevated levels in calves with *1501 or neutral alleles after seroconversion. Leukocyte and lymphocyte counts and BLVp24 expression did not significantly differ between genetic groups. Animals with <20 proviral copies/30 ng DNA at 178 dpi or < 200 proviral copies at 88 dpi were classified as LPL, while calves with levels above these limits were considered as high proviral load (HPL) profile. All 6 calves with*1501 allele progressed to HPL, while LPL was attained by 6/7 (86%) and 2/6 (33%) of the calves with *0902 and neutral alleles respectively. One calf with both *0902 and *1501 developed LPL. This is the first experimental induction of LPL profile in cattle.Fil: Forletti, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Lützelschwab, Claudia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Cepeda, Rosana Esther. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Exactas. Instituto Multidisciplinario de Ecosistemas y Desarrollo Sustentable; ArgentinaFil: Esteban, Eduardo Néstor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Gutiérrez, Silvina Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaBioMed Central2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/257293Forletti, Agustina; Lützelschwab, Claudia María; Cepeda, Rosana Esther; Esteban, Eduardo Néstor; Gutiérrez, Silvina Elena; Early events following bovine leukaemia virus infection in calves with different alleles of the major histocompatibility complex  DRB3 gene; BioMed Central; Veterinary Research; 51; 1; 1-2020; 1-121297-9716CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-019-0732-1info:eu-repo/semantics/altIdentifier/doi/10.1186/s13567-019-0732-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:17Zoai:ri.conicet.gov.ar:11336/257293instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:17.588CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Early events following bovine leukaemia virus infection in calves with different alleles of the major histocompatibility complex  DRB3 gene
title Early events following bovine leukaemia virus infection in calves with different alleles of the major histocompatibility complex  DRB3 gene
spellingShingle Early events following bovine leukaemia virus infection in calves with different alleles of the major histocompatibility complex  DRB3 gene
Forletti, Agustina
BOVINE LEUKEMIA VIRUS
PROVIRAL LOAD
GENETIC RESISTANCE
BOLA-DRB3
MAJOR HISTOCOMPATIBILITY COMPLEX
EXPERIMENTAL INFECTION
QPCR
BLVP24
BLVGP51
title_short Early events following bovine leukaemia virus infection in calves with different alleles of the major histocompatibility complex  DRB3 gene
title_full Early events following bovine leukaemia virus infection in calves with different alleles of the major histocompatibility complex  DRB3 gene
title_fullStr Early events following bovine leukaemia virus infection in calves with different alleles of the major histocompatibility complex  DRB3 gene
title_full_unstemmed Early events following bovine leukaemia virus infection in calves with different alleles of the major histocompatibility complex  DRB3 gene
title_sort Early events following bovine leukaemia virus infection in calves with different alleles of the major histocompatibility complex  DRB3 gene
dc.creator.none.fl_str_mv Forletti, Agustina
Lützelschwab, Claudia María
Cepeda, Rosana Esther
Esteban, Eduardo Néstor
Gutiérrez, Silvina Elena
author Forletti, Agustina
author_facet Forletti, Agustina
Lützelschwab, Claudia María
Cepeda, Rosana Esther
Esteban, Eduardo Néstor
Gutiérrez, Silvina Elena
author_role author
author2 Lützelschwab, Claudia María
Cepeda, Rosana Esther
Esteban, Eduardo Néstor
Gutiérrez, Silvina Elena
author2_role author
author
author
author
dc.subject.none.fl_str_mv BOVINE LEUKEMIA VIRUS
PROVIRAL LOAD
GENETIC RESISTANCE
BOLA-DRB3
MAJOR HISTOCOMPATIBILITY COMPLEX
EXPERIMENTAL INFECTION
QPCR
BLVP24
BLVGP51
topic BOVINE LEUKEMIA VIRUS
PROVIRAL LOAD
GENETIC RESISTANCE
BOLA-DRB3
MAJOR HISTOCOMPATIBILITY COMPLEX
EXPERIMENTAL INFECTION
QPCR
BLVP24
BLVGP51
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Cattle maintaining a low proviral load (LPL) status after Bovine Leukemia Virus (BLV) infection have been recognized as BLV controllers, and non-transmitters to uninfected cattle in experimental and natural conditions. LPL has been associated with host genetics, mainly with BoLAclass II DRB3 gene. The aim of this work was to study the kinetics of the BLV and the host response in Holstein calves carrying different BoLA-DRB3 alleles. Twenty BLV free calves were inoculated with infected lymphocytes. Two calves were maintained uninfected as controls. Proviral load, total leukocyte and lymphocyte counts, anti-BLVgp51 titers and BLVp24 expression were determined in blood samples at various times post inoculation (dpi).Proviral loadpeaked at 30 dpi in all animals. It declined steadily from seroconversion (38 dpi) to the end of the study (178 dpi) in calves carrying resistance-associated allele (*0902), while it was maintained at elevated levels in calves with *1501 or neutral alleles after seroconversion. Leukocyte and lymphocyte counts and BLVp24 expression did not significantly differ between genetic groups. Animals with <20 proviral copies/30 ng DNA at 178 dpi or < 200 proviral copies at 88 dpi were classified as LPL, while calves with levels above these limits were considered as high proviral load (HPL) profile. All 6 calves with*1501 allele progressed to HPL, while LPL was attained by 6/7 (86%) and 2/6 (33%) of the calves with *0902 and neutral alleles respectively. One calf with both *0902 and *1501 developed LPL. This is the first experimental induction of LPL profile in cattle.
Fil: Forletti, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lützelschwab, Claudia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Cepeda, Rosana Esther. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Exactas. Instituto Multidisciplinario de Ecosistemas y Desarrollo Sustentable; Argentina
Fil: Esteban, Eduardo Néstor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Gutiérrez, Silvina Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
description Cattle maintaining a low proviral load (LPL) status after Bovine Leukemia Virus (BLV) infection have been recognized as BLV controllers, and non-transmitters to uninfected cattle in experimental and natural conditions. LPL has been associated with host genetics, mainly with BoLAclass II DRB3 gene. The aim of this work was to study the kinetics of the BLV and the host response in Holstein calves carrying different BoLA-DRB3 alleles. Twenty BLV free calves were inoculated with infected lymphocytes. Two calves were maintained uninfected as controls. Proviral load, total leukocyte and lymphocyte counts, anti-BLVgp51 titers and BLVp24 expression were determined in blood samples at various times post inoculation (dpi).Proviral loadpeaked at 30 dpi in all animals. It declined steadily from seroconversion (38 dpi) to the end of the study (178 dpi) in calves carrying resistance-associated allele (*0902), while it was maintained at elevated levels in calves with *1501 or neutral alleles after seroconversion. Leukocyte and lymphocyte counts and BLVp24 expression did not significantly differ between genetic groups. Animals with <20 proviral copies/30 ng DNA at 178 dpi or < 200 proviral copies at 88 dpi were classified as LPL, while calves with levels above these limits were considered as high proviral load (HPL) profile. All 6 calves with*1501 allele progressed to HPL, while LPL was attained by 6/7 (86%) and 2/6 (33%) of the calves with *0902 and neutral alleles respectively. One calf with both *0902 and *1501 developed LPL. This is the first experimental induction of LPL profile in cattle.
publishDate 2020
dc.date.none.fl_str_mv 2020-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/257293
Forletti, Agustina; Lützelschwab, Claudia María; Cepeda, Rosana Esther; Esteban, Eduardo Néstor; Gutiérrez, Silvina Elena; Early events following bovine leukaemia virus infection in calves with different alleles of the major histocompatibility complex  DRB3 gene; BioMed Central; Veterinary Research; 51; 1; 1-2020; 1-12
1297-9716
CONICET Digital
CONICET
url http://hdl.handle.net/11336/257293
identifier_str_mv Forletti, Agustina; Lützelschwab, Claudia María; Cepeda, Rosana Esther; Esteban, Eduardo Néstor; Gutiérrez, Silvina Elena; Early events following bovine leukaemia virus infection in calves with different alleles of the major histocompatibility complex  DRB3 gene; BioMed Central; Veterinary Research; 51; 1; 1-2020; 1-12
1297-9716
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-019-0732-1
info:eu-repo/semantics/altIdentifier/doi/10.1186/s13567-019-0732-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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