Redox systemic signaling and induced tolerance responses during soybean–Bradyrhizobium japonicum interaction: Involvement of nod factor receptor and autoregulation of nodulation

Autores
Fernandez Göbel, Tadeo Francisco; Deanna, Rocío; Muñoz, Nacira Belén; Robert, Germán; Asurmendi, Sebastian; Lascano, Hernan Ramiro
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The symbiotic relationship between legumes and nitrogen-fixing rhizobia induces local and systemic responses, which ultimately lead to nodule formation. The autoregulation of nodulation (AON) is a systemic mechanism related to innate immunity that controls nodule development and involves different components ranging from hormones, peptides, receptors to small RNAs. Here, we characterized a rapid systemic redox changes induced during soybean?Bradyrhizobium japonicum symbiotic interaction. A transient peak of reactive oxygen species (ROS) generation was found in soybean leaves after 30 min of root inoculation with B. japonicum. The ROS response was accompanied by changes in the redox state of glutathione and by activation of antioxidant enzymes. Moreover, the ROS peak and antioxidant enzyme activation were abolished in leaves by the addition, in either root or leaf, of DPI, an NADPH oxidase inhibitor. Likewise, these systemic redox changes primed the plant increasing its tolerance to photooxidative stress. With the use of non-nodulating nfr5-mutant and hyper-nodulating nark-mutant soybean plants, we subsequently studied the systemic redox changes. The nfr5-mutant lacked the systemic redox changes after inoculation, whereas the nark-mutant showed a similar redox systemic signaling than the wild type plants. However, neither nfr5- nor nark-mutant exhibited tolerance to photooxidative stress condition. Altogether, these results demonstrated that (i) the early redox systemic signaling during symbiotic interaction depends on a Nod factor receptor, and that (ii) the induced tolerance response depends on the AON mechanisms.
Fil: Fernandez Göbel, Tadeo Francisco. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Deanna, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Muñoz, Nacira Belén. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Robert, Germán. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Botánica. Laboratorio de Fisiología Vegetal; Argentina
Fil: Asurmendi, Sebastian. Universidad Nacional de Córdoba; Argentina
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Materia
AUTOREGULATION OF NODULATION
ISR/PGPR-LIKE RESPONSE
REDOX SIGNALING
RHIZOBIA
SOYBEAN SYMBIOSIS
SYSTEMIC CHANGES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/109560
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/109560
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Redox systemic signaling and induced tolerance responses during soybean–Bradyrhizobium japonicum interaction: Involvement of nod factor receptor and autoregulation of nodulationFernandez Göbel, Tadeo FranciscoDeanna, RocíoMuñoz, Nacira BelénRobert, GermánAsurmendi, SebastianLascano, Hernan RamiroAUTOREGULATION OF NODULATIONISR/PGPR-LIKE RESPONSEREDOX SIGNALINGRHIZOBIASOYBEAN SYMBIOSISSYSTEMIC CHANGEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The symbiotic relationship between legumes and nitrogen-fixing rhizobia induces local and systemic responses, which ultimately lead to nodule formation. The autoregulation of nodulation (AON) is a systemic mechanism related to innate immunity that controls nodule development and involves different components ranging from hormones, peptides, receptors to small RNAs. Here, we characterized a rapid systemic redox changes induced during soybean?Bradyrhizobium japonicum symbiotic interaction. A transient peak of reactive oxygen species (ROS) generation was found in soybean leaves after 30 min of root inoculation with B. japonicum. The ROS response was accompanied by changes in the redox state of glutathione and by activation of antioxidant enzymes. Moreover, the ROS peak and antioxidant enzyme activation were abolished in leaves by the addition, in either root or leaf, of DPI, an NADPH oxidase inhibitor. Likewise, these systemic redox changes primed the plant increasing its tolerance to photooxidative stress. With the use of non-nodulating nfr5-mutant and hyper-nodulating nark-mutant soybean plants, we subsequently studied the systemic redox changes. The nfr5-mutant lacked the systemic redox changes after inoculation, whereas the nark-mutant showed a similar redox systemic signaling than the wild type plants. However, neither nfr5- nor nark-mutant exhibited tolerance to photooxidative stress condition. Altogether, these results demonstrated that (i) the early redox systemic signaling during symbiotic interaction depends on a Nod factor receptor, and that (ii) the induced tolerance response depends on the AON mechanisms.Fil: Fernandez Göbel, Tadeo Francisco. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Deanna, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Muñoz, Nacira Belén. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Robert, Germán. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Botánica. Laboratorio de Fisiología Vegetal; ArgentinaFil: Asurmendi, Sebastian. Universidad Nacional de Córdoba; ArgentinaFil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFrontiers Media S.A.2019-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/109560Fernandez Göbel, Tadeo Francisco; Deanna, Rocío; Muñoz, Nacira Belén; Robert, Germán; Asurmendi, Sebastian; et al.; Redox systemic signaling and induced tolerance responses during soybean–Bradyrhizobium japonicum interaction: Involvement of nod factor receptor and autoregulation of nodulation; Frontiers Media S.A.; Frontiers in Plant Science; 10; 2-20191664-462XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2019.00141/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2019.00141info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:31:54Zoai:ri.conicet.gov.ar:11336/109560instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:31:55.226CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Redox systemic signaling and induced tolerance responses during soybean–Bradyrhizobium japonicum interaction: Involvement of nod factor receptor and autoregulation of nodulation
title Redox systemic signaling and induced tolerance responses during soybean–Bradyrhizobium japonicum interaction: Involvement of nod factor receptor and autoregulation of nodulation
spellingShingle Redox systemic signaling and induced tolerance responses during soybean–Bradyrhizobium japonicum interaction: Involvement of nod factor receptor and autoregulation of nodulation
Fernandez Göbel, Tadeo Francisco
AUTOREGULATION OF NODULATION
ISR/PGPR-LIKE RESPONSE
REDOX SIGNALING
RHIZOBIA
SOYBEAN SYMBIOSIS
SYSTEMIC CHANGES
title_short Redox systemic signaling and induced tolerance responses during soybean–Bradyrhizobium japonicum interaction: Involvement of nod factor receptor and autoregulation of nodulation
title_full Redox systemic signaling and induced tolerance responses during soybean–Bradyrhizobium japonicum interaction: Involvement of nod factor receptor and autoregulation of nodulation
title_fullStr Redox systemic signaling and induced tolerance responses during soybean–Bradyrhizobium japonicum interaction: Involvement of nod factor receptor and autoregulation of nodulation
title_full_unstemmed Redox systemic signaling and induced tolerance responses during soybean–Bradyrhizobium japonicum interaction: Involvement of nod factor receptor and autoregulation of nodulation
title_sort Redox systemic signaling and induced tolerance responses during soybean–Bradyrhizobium japonicum interaction: Involvement of nod factor receptor and autoregulation of nodulation
dc.creator.none.fl_str_mv Fernandez Göbel, Tadeo Francisco
Deanna, Rocío
Muñoz, Nacira Belén
Robert, Germán
Asurmendi, Sebastian
Lascano, Hernan Ramiro
author Fernandez Göbel, Tadeo Francisco
author_facet Fernandez Göbel, Tadeo Francisco
Deanna, Rocío
Muñoz, Nacira Belén
Robert, Germán
Asurmendi, Sebastian
Lascano, Hernan Ramiro
author_role author
author2 Deanna, Rocío
Muñoz, Nacira Belén
Robert, Germán
Asurmendi, Sebastian
Lascano, Hernan Ramiro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv AUTOREGULATION OF NODULATION
ISR/PGPR-LIKE RESPONSE
REDOX SIGNALING
RHIZOBIA
SOYBEAN SYMBIOSIS
SYSTEMIC CHANGES
topic AUTOREGULATION OF NODULATION
ISR/PGPR-LIKE RESPONSE
REDOX SIGNALING
RHIZOBIA
SOYBEAN SYMBIOSIS
SYSTEMIC CHANGES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The symbiotic relationship between legumes and nitrogen-fixing rhizobia induces local and systemic responses, which ultimately lead to nodule formation. The autoregulation of nodulation (AON) is a systemic mechanism related to innate immunity that controls nodule development and involves different components ranging from hormones, peptides, receptors to small RNAs. Here, we characterized a rapid systemic redox changes induced during soybean?Bradyrhizobium japonicum symbiotic interaction. A transient peak of reactive oxygen species (ROS) generation was found in soybean leaves after 30 min of root inoculation with B. japonicum. The ROS response was accompanied by changes in the redox state of glutathione and by activation of antioxidant enzymes. Moreover, the ROS peak and antioxidant enzyme activation were abolished in leaves by the addition, in either root or leaf, of DPI, an NADPH oxidase inhibitor. Likewise, these systemic redox changes primed the plant increasing its tolerance to photooxidative stress. With the use of non-nodulating nfr5-mutant and hyper-nodulating nark-mutant soybean plants, we subsequently studied the systemic redox changes. The nfr5-mutant lacked the systemic redox changes after inoculation, whereas the nark-mutant showed a similar redox systemic signaling than the wild type plants. However, neither nfr5- nor nark-mutant exhibited tolerance to photooxidative stress condition. Altogether, these results demonstrated that (i) the early redox systemic signaling during symbiotic interaction depends on a Nod factor receptor, and that (ii) the induced tolerance response depends on the AON mechanisms.
Fil: Fernandez Göbel, Tadeo Francisco. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Deanna, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Muñoz, Nacira Belén. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Robert, Germán. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Botánica. Laboratorio de Fisiología Vegetal; Argentina
Fil: Asurmendi, Sebastian. Universidad Nacional de Córdoba; Argentina
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
description The symbiotic relationship between legumes and nitrogen-fixing rhizobia induces local and systemic responses, which ultimately lead to nodule formation. The autoregulation of nodulation (AON) is a systemic mechanism related to innate immunity that controls nodule development and involves different components ranging from hormones, peptides, receptors to small RNAs. Here, we characterized a rapid systemic redox changes induced during soybean?Bradyrhizobium japonicum symbiotic interaction. A transient peak of reactive oxygen species (ROS) generation was found in soybean leaves after 30 min of root inoculation with B. japonicum. The ROS response was accompanied by changes in the redox state of glutathione and by activation of antioxidant enzymes. Moreover, the ROS peak and antioxidant enzyme activation were abolished in leaves by the addition, in either root or leaf, of DPI, an NADPH oxidase inhibitor. Likewise, these systemic redox changes primed the plant increasing its tolerance to photooxidative stress. With the use of non-nodulating nfr5-mutant and hyper-nodulating nark-mutant soybean plants, we subsequently studied the systemic redox changes. The nfr5-mutant lacked the systemic redox changes after inoculation, whereas the nark-mutant showed a similar redox systemic signaling than the wild type plants. However, neither nfr5- nor nark-mutant exhibited tolerance to photooxidative stress condition. Altogether, these results demonstrated that (i) the early redox systemic signaling during symbiotic interaction depends on a Nod factor receptor, and that (ii) the induced tolerance response depends on the AON mechanisms.
publishDate 2019
dc.date.none.fl_str_mv 2019-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/109560
Fernandez Göbel, Tadeo Francisco; Deanna, Rocío; Muñoz, Nacira Belén; Robert, Germán; Asurmendi, Sebastian; et al.; Redox systemic signaling and induced tolerance responses during soybean–Bradyrhizobium japonicum interaction: Involvement of nod factor receptor and autoregulation of nodulation; Frontiers Media S.A.; Frontiers in Plant Science; 10; 2-2019
1664-462X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/109560
identifier_str_mv Fernandez Göbel, Tadeo Francisco; Deanna, Rocío; Muñoz, Nacira Belén; Robert, Germán; Asurmendi, Sebastian; et al.; Redox systemic signaling and induced tolerance responses during soybean–Bradyrhizobium japonicum interaction: Involvement of nod factor receptor and autoregulation of nodulation; Frontiers Media S.A.; Frontiers in Plant Science; 10; 2-2019
1664-462X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2019.00141/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2019.00141
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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