A new PGPR co-inoculated with Bradyrhizobium japonicum enhances soybean nodulation

Autores
Masciarelli, Oscar Alberto; Llanes, Analia Susana; Luna, Maria Virginia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new PGPR (plant growth promoting rhizobacteria) strain was isolated from soybean seeds and the bacterial mechanisms related to plant growth promotion were evaluated and characterized. Isolates were genotypically compared and identified by amplification of partial sequences of 16S DNAr as Bacillus amyloliquefaciens strain LL2012. Isolates were grown until exponential growth phase to evaluate the atmospheric nitrogen fixation, enzymatic activities, phosphate solubilization, siderophores and phytohormones production. LL2012 strain was able to grow and to produce high levels of auxin, gibberellins and salicylic acid in chemically defined medium. Co-inoculation of soybean plants with LL2012 strain and the natural symbiont (Bradyrhizobium japonicum) altered plant growth parameters and significantly improved nodulation. Our results show that the association of LL2012 with B. japonicum, enhanced the capacity of the latter to colonize plant roots and increase the number of nodules, which make the co-inoculation technique attractive for use in commercial inoculant formulations following proper field evaluation.
Fil: Masciarelli, Oscar Alberto. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular. Laboratorio de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Llanes, Analia Susana. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular. Laboratorio de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Luna, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular. Laboratorio de Fisiología Vegetal; Argentina
Materia
BACILLUS AMYLOLIQUEFACIENS
CO-INOCULATION
PGPR
PHYTOHORMONES
SOYBEAN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33607
Acceder
id CONICETDig_f2ef4cf858a87720272f63a97d500729
oai_identifier_str oai:ri.conicet.gov.ar:11336/33607
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network_name_str CONICET Digital (CONICET)
spelling A new PGPR co-inoculated with Bradyrhizobium japonicum enhances soybean nodulationMasciarelli, Oscar AlbertoLlanes, Analia SusanaLuna, Maria VirginiaBACILLUS AMYLOLIQUEFACIENSCO-INOCULATIONPGPRPHYTOHORMONESSOYBEANhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A new PGPR (plant growth promoting rhizobacteria) strain was isolated from soybean seeds and the bacterial mechanisms related to plant growth promotion were evaluated and characterized. Isolates were genotypically compared and identified by amplification of partial sequences of 16S DNAr as Bacillus amyloliquefaciens strain LL2012. Isolates were grown until exponential growth phase to evaluate the atmospheric nitrogen fixation, enzymatic activities, phosphate solubilization, siderophores and phytohormones production. LL2012 strain was able to grow and to produce high levels of auxin, gibberellins and salicylic acid in chemically defined medium. Co-inoculation of soybean plants with LL2012 strain and the natural symbiont (Bradyrhizobium japonicum) altered plant growth parameters and significantly improved nodulation. Our results show that the association of LL2012 with B. japonicum, enhanced the capacity of the latter to colonize plant roots and increase the number of nodules, which make the co-inoculation technique attractive for use in commercial inoculant formulations following proper field evaluation.Fil: Masciarelli, Oscar Alberto. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular. Laboratorio de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Llanes, Analia Susana. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular. Laboratorio de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Luna, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular. Laboratorio de Fisiología Vegetal; ArgentinaElsevier Gmbh2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33607Masciarelli, Oscar Alberto; Llanes, Analia Susana; Luna, Maria Virginia; A new PGPR co-inoculated with Bradyrhizobium japonicum enhances soybean nodulation; Elsevier Gmbh; Microbiological Research; 169; 7-8; 1-2014; 609-6150944-5013CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.micres.2013.10.001info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0944501313001705info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:57:50Zoai:ri.conicet.gov.ar:11336/33607instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:57:50.942CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A new PGPR co-inoculated with Bradyrhizobium japonicum enhances soybean nodulation
title A new PGPR co-inoculated with Bradyrhizobium japonicum enhances soybean nodulation
spellingShingle A new PGPR co-inoculated with Bradyrhizobium japonicum enhances soybean nodulation
Masciarelli, Oscar Alberto
BACILLUS AMYLOLIQUEFACIENS
CO-INOCULATION
PGPR
PHYTOHORMONES
SOYBEAN
title_short A new PGPR co-inoculated with Bradyrhizobium japonicum enhances soybean nodulation
title_full A new PGPR co-inoculated with Bradyrhizobium japonicum enhances soybean nodulation
title_fullStr A new PGPR co-inoculated with Bradyrhizobium japonicum enhances soybean nodulation
title_full_unstemmed A new PGPR co-inoculated with Bradyrhizobium japonicum enhances soybean nodulation
title_sort A new PGPR co-inoculated with Bradyrhizobium japonicum enhances soybean nodulation
dc.creator.none.fl_str_mv Masciarelli, Oscar Alberto
Llanes, Analia Susana
Luna, Maria Virginia
author Masciarelli, Oscar Alberto
author_facet Masciarelli, Oscar Alberto
Llanes, Analia Susana
Luna, Maria Virginia
author_role author
author2 Llanes, Analia Susana
Luna, Maria Virginia
author2_role author
author
dc.subject.none.fl_str_mv BACILLUS AMYLOLIQUEFACIENS
CO-INOCULATION
PGPR
PHYTOHORMONES
SOYBEAN
topic BACILLUS AMYLOLIQUEFACIENS
CO-INOCULATION
PGPR
PHYTOHORMONES
SOYBEAN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A new PGPR (plant growth promoting rhizobacteria) strain was isolated from soybean seeds and the bacterial mechanisms related to plant growth promotion were evaluated and characterized. Isolates were genotypically compared and identified by amplification of partial sequences of 16S DNAr as Bacillus amyloliquefaciens strain LL2012. Isolates were grown until exponential growth phase to evaluate the atmospheric nitrogen fixation, enzymatic activities, phosphate solubilization, siderophores and phytohormones production. LL2012 strain was able to grow and to produce high levels of auxin, gibberellins and salicylic acid in chemically defined medium. Co-inoculation of soybean plants with LL2012 strain and the natural symbiont (Bradyrhizobium japonicum) altered plant growth parameters and significantly improved nodulation. Our results show that the association of LL2012 with B. japonicum, enhanced the capacity of the latter to colonize plant roots and increase the number of nodules, which make the co-inoculation technique attractive for use in commercial inoculant formulations following proper field evaluation.
Fil: Masciarelli, Oscar Alberto. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular. Laboratorio de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Llanes, Analia Susana. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular. Laboratorio de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Luna, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular. Laboratorio de Fisiología Vegetal; Argentina
description A new PGPR (plant growth promoting rhizobacteria) strain was isolated from soybean seeds and the bacterial mechanisms related to plant growth promotion were evaluated and characterized. Isolates were genotypically compared and identified by amplification of partial sequences of 16S DNAr as Bacillus amyloliquefaciens strain LL2012. Isolates were grown until exponential growth phase to evaluate the atmospheric nitrogen fixation, enzymatic activities, phosphate solubilization, siderophores and phytohormones production. LL2012 strain was able to grow and to produce high levels of auxin, gibberellins and salicylic acid in chemically defined medium. Co-inoculation of soybean plants with LL2012 strain and the natural symbiont (Bradyrhizobium japonicum) altered plant growth parameters and significantly improved nodulation. Our results show that the association of LL2012 with B. japonicum, enhanced the capacity of the latter to colonize plant roots and increase the number of nodules, which make the co-inoculation technique attractive for use in commercial inoculant formulations following proper field evaluation.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33607
Masciarelli, Oscar Alberto; Llanes, Analia Susana; Luna, Maria Virginia; A new PGPR co-inoculated with Bradyrhizobium japonicum enhances soybean nodulation; Elsevier Gmbh; Microbiological Research; 169; 7-8; 1-2014; 609-615
0944-5013
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33607
identifier_str_mv Masciarelli, Oscar Alberto; Llanes, Analia Susana; Luna, Maria Virginia; A new PGPR co-inoculated with Bradyrhizobium japonicum enhances soybean nodulation; Elsevier Gmbh; Microbiological Research; 169; 7-8; 1-2014; 609-615
0944-5013
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micres.2013.10.001
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0944501313001705
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083117840859136
score 13.22299

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